Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,237,091 T→A 18.4% Q337L (CAG→CTG)  uxaC ← uronate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,237,0910TA18.4% 754.0 / 157.1 283Q337L (CAG→CTG) uxaCuronate isomerase
Reads supporting (aligned to +/- strand):  ref base T (104/127);  new base A (32/20);  total (136/147)
Fisher's exact test for biased strand distribution p-value = 3.28e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 300 total aligned reads displayed.

TCTGATCGTTAAACCACCAGCCGGAACCGAACTGCATTTTGCCCGGCATACCTTCGCCCTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGTACCCAGCCGCGACGTGC  >  CP009273/3236942‑3237239
                                                                                                                                                     |                                                                                                                                                    
tCTGATCGTTAAACCACCAGCCGGAACCGAACTGCATTTTGCCCGGCATACCTTCGCCCTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCt                                                                                                                                                      <  1:442474/150‑1 (MQ=255)
 cTGATCGTTAAACCACCAGCCGGAACCGAACTGCATTTTGCCCGGCATACCTTCGCCCTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCtg                                                                                                                                                     >  1:2306534/1‑150 (MQ=255)
   gATCGTTAAACCACCAGCCGGAACCGAACTGCATTTTGCCCGGCATACCTTCGCCCTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCtgct                                                                                                                                                   >  1:4752899/1‑150 (MQ=255)
   gATCGTTAAACCACCAGCCGGAACCGAACTGCATTTTGCCCGGCATACCTTCGCCCTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCtgct                                                                                                                                                   >  2:4143086/1‑150 (MQ=255)
    aTCGTTAAACCACCAGCCGGAACCGAACTGCATTTTGCCCGGCATACCTTCGCCCTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCtgctt                                                                                                                                                  <  2:107497/150‑1 (MQ=255)
       gTTAAACCACCAGCCGGAACCGAACTGCATTTTGCCCGGCATACCTTCGCCCTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCtgcttgct                                                                                                                                               >  1:46416/1‑150 (MQ=255)
       gTTAAACCACCAGCCGGAACCGAACTGCATTTTGCCCGGCATACCTTCGCCCTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCtgcttgct                                                                                                                                               >  1:3888937/1‑150 (MQ=255)
         tAAACCACCAGCCGGAACCGAACTGCATTTTGCCCGGCATACCTTCGCCCTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCa                                                                                                                                             <  2:110499/150‑1 (MQ=255)
          aaaCCACCAGCCGGAACCGAACTGCATTTTGCCCGGCATACCTTCGCCCTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAg                                                                                                                                            <  1:4083097/150‑1 (MQ=255)
          aaaCCACCAGCCGGAACCGAACTGCATTTTGCCCGGCATACCTTCGCCCTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAg                                                                                                                                            <  1:3209569/150‑1 (MQ=255)
           aaCCACCAGCCGGAACCGAACTGCATTTTGCCCGGCATACCTTCGCCCTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGc                                                                                                                                           >  1:1026264/1‑150 (MQ=255)
                  aGCCGGAACCGAACTGCATTTTGCCCGGCATACCTTCGCCCTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAg                                                                                                                                    >  2:2373012/1‑150 (MQ=255)
                   gCCGGAACCGAACTGCATTTTGCCCGGCATACCTTCGCCCTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGa                                                                                                                                   <  2:816250/150‑1 (MQ=255)
                     cGGAACCGAACTGCATTTTGCCCGGCATACCTTCGCCCTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACa                                                                                                                                 >  2:4824037/1‑150 (MQ=255)
                     cGGAACCGAACTGCATTTTGCCCGGCATACCGTCGCCCTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCAGCTTGCTCAGCAACTTAGACa                                                                                                                                 <  2:3459523/150‑1 (MQ=255)
                       gAACCGAACTGCATTTTGCCCGGCATACCTTCGCCCTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGc                                                                                                                               >  1:2057691/1‑150 (MQ=255)
                         aCCGAACTGCATTTTGCCCGGCATACCTTCGCCCTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGctc                                                                                                                             <  1:4438120/150‑1 (MQ=255)
                            gAACTGCATTTTGCCCGGCATACCTTCGCCCTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTc                                                                                                                          <  1:2730112/150‑1 (MQ=255)
                            gAACTGCATTTTGCCCGGCATACCTTCGCCCTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTc                                                                                                                          >  1:539146/1‑150 (MQ=255)
                             aaCTGCATTTTGCCCGGCATACCTTCGCCCTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTcc                                                                                                                         <  2:1942276/150‑1 (MQ=255)
                                tGCATTTTGCCCGGCATACCTTCGCCCTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCAGCTTGCTCAGCAACTTAGACAGCTCTTCCGcc                                                                                                                      >  1:1727438/1‑150 (MQ=255)
                                tGCATTTTGCCCGGCATACCTTCGCCCTGGAAGTAACCGATCATGGTGCCCAGCACTTCGATATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGcc                                                                                                                      >  1:1466483/1‑150 (MQ=255)
                                   aTTTTGCCCGGCATACCTTCGCCCTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATc                                                                                                                   <  1:4857871/150‑1 (MQ=255)
                                    ttttGCCCGGCATACCTTCGCCCTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCAGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCg                                                                                                                  >  1:1394810/1‑150 (MQ=255)
                                        gCCCGGCATACCTTCGCCCTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACg                                                                                                              >  1:1647864/1‑150 (MQ=255)
                                         cccGGCATACCTTCGCCCTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACgg                                                                                                             >  2:1001054/1‑150 (MQ=255)
                                         cccGGCATACCTTCGCCCTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACgg                                                                                                             >  2:4190841/1‑150 (MQ=255)
                                         cccGGCATACCTTCGCCCTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACgg                                                                                                             >  2:2872645/1‑150 (MQ=255)
                                          ccGGCATACCTTCGCCCTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGt                                                                                                            <  2:3865892/150‑1 (MQ=255)
                                              cATACCTTCGCCCTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGtt                                                                                                        <  1:2825413/150‑1 (MQ=255)
                                              cATACCTTCGCCCTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGtt                                                                                                        <  1:3750126/150‑1 (MQ=255)
                                              cATACCTTCGCCCTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCAGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGtt                                                                                                        <  2:3889345/150‑1 (MQ=255)
                                               aTACCTTCGCCCTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTg                                                                                                       <  1:4062064/150‑1 (MQ=255)
                                                  ccTTCGCCCTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATg                                                                                                    >  1:1612176/1‑150 (MQ=255)
                                                   cTTCGCCCTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATgg                                                                                                   >  1:3310713/1‑150 (MQ=255)
                                                    ttCGCCCTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGa                                                                                                  <  1:1989817/150‑1 (MQ=255)
                                                     tCGCCCTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAg                                                                                                 <  2:3313667/150‑1 (MQ=255)
                                                       gCCCTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTc                                                                                               <  2:1721278/150‑1 (MQ=255)
                                                       gCCCTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTc                                                                                               <  2:1034376/150‑1 (MQ=255)
                                                        cccTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCa                                                                                              >  1:4203702/1‑150 (MQ=255)
                                                            ggAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGc                                                                                          >  1:1975573/1‑150 (MQ=255)
                                                            ggAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGc                                                                                          >  1:2417715/1‑150 (MQ=255)
                                                             gAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGcc                                                                                         <  1:425559/150‑1 (MQ=255)
                                                             gAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCAGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGcc                                                                                         >  1:2351992/1‑150 (MQ=255)
                                                             gAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCAGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGcc                                                                                         >  1:3924599/1‑150 (MQ=255)
                                                                   aCCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATc                                                                                   >  1:4820713/1‑150 (MQ=255)
                                                                   aCCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATc                                                                                   >  2:3252236/1‑150 (MQ=255)
                                                                   aCCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATc                                                                                   >  2:544169/1‑150 (MQ=255)
                                                                   aCCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATc                                                                                   >  2:4697160/1‑150 (MQ=255)
                                                                    ccGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATcc                                                                                  <  1:3638338/150‑1 (MQ=255)
                                                                    ccGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCAGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATcc                                                                                  <  1:1276027/150‑1 (MQ=255)
                                                                     cGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCg                                                                                 <  2:2860546/150‑1 (MQ=255)
                                                                     cGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCg                                                                                 <  2:428684/150‑1 (MQ=255)
                                                                      gATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCgg                                                                                >  2:677043/1‑150 (MQ=255)
                                                                      gATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCgg                                                                                <  2:4498298/150‑1 (MQ=255)
                                                                      gATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCAGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCgg                                                                                >  1:66926/1‑150 (MQ=255)
                                                                          aTGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGcccc                                                                            <  1:3048822/150‑1 (MQ=255)
                                                                           tGGTGCCCAGCACTTCGTTATCGCGCTGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCTGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCTGCCCca                                                                           <  2:4447204/150‑1 (MQ=255)
                                                                            ggTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCcag                                                                          <  2:4273687/150‑1 (MQ=255)
                                                                                cccAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGt                                                                      <  2:1394100/150‑1 (MQ=255)
                                                                                     cACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGaa                                                                 >  1:970486/1‑150 (MQ=255)
                                                                                      aCTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAAc                                                                <  1:3144335/150‑1 (MQ=255)
                                                                                       cTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCAGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACt                                                               <  1:4155575/150‑1 (MQ=255)
                                                                                          cGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGAc                                                            <  2:245481/150‑1 (MQ=255)
                                                                                          cGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCAGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGAc                                                            >  2:2529507/1‑150 (MQ=255)
                                                                                            ttATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCAGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGc                                                          <  2:1231620/150‑1 (MQ=255)
                                                                                              aTCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAg                                                        >  1:4007409/1‑150 (MQ=255)
                                                                                               tCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAgg                                                       >  2:2917279/1‑150 (MQ=255)
                                                                                                  cgcgGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGgttg                                                    <  1:3007730/150‑1 (MQ=255)
                                                                                                    cgGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCAGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGgttgtt                                                  <  2:154883/150‑1 (MQ=255)
                                                                                                       gTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGttatt                                               <  2:2184910/150‑1 (MQ=255)
                                                                                                       gTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGttatt                                               <  1:2523571/150‑1 (MQ=255)
                                                                                                         tCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTAc                                             >  1:2925345/1‑150 (MQ=255)
                                                                                                         tCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTAc                                             <  2:3092016/150‑1 (MQ=255)
                                                                                                         tCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCAGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTAc                                             >  1:2162410/1‑150 (MQ=255)
                                                                                                          cAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACg                                            >  1:2501601/1‑150 (MQ=255)
                                                                                                           aGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCAGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGc                                           >  2:2280828/1‑150 (MQ=255)
                                                                                                               aGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAgcg                                       >  2:3828834/1‑150 (MQ=255)
                                                                                                                 tACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAgcgcg                                     >  2:2678645/1‑150 (MQ=255)
                                                                                                                 tACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCAGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAgcgcg                                     >  2:1817615/1‑150 (MQ=255)
                                                                                                                    aGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCa                                  <  1:1925738/150‑1 (MQ=255)
                                                                                                                     gAATGGTTTTCGGCAGCAGGTTTTCTTCATTCAGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGTACTGACGCAGGTTGTTATTACGCAGCGCGCCaa                                 <  2:1037777/150‑1 (MQ=255)
                                                                                                                      aaTGGTTTTCGGCAGCAGGTTTTCTTCATTCAGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAAt                                >  2:925048/1‑150 (MQ=255)
                                                                                                                      aaTGGTTTTCGGCAGCAGGTTTTCTTCATTCAGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAAt                                >  2:3364554/1‑150 (MQ=255)
                                                                                                                         ggTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAAtgtg                             <  2:876585/150‑1 (MQ=255)
                                                                                                                          gTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATTTgg                            >  1:2466811/1‑150 (MQ=255)
                                                                                                                             ttCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTAc                         <  2:4028816/150‑1 (MQ=255)
                                                                                                                              tCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTAct                        >  1:3330884/1‑150 (MQ=255)
                                                                                                                               cGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTActg                       >  1:1828552/1‑150 (MQ=255)
                                                                                                                                ggCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGCGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAAAGTGGTActgc                      >  1:2699156/1‑150 (MQ=255)
                                                                                                                                   agcagGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGt                   <  2:1647864/150‑1 (MQ=255)
                                                                                                                                   agcagGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGt                   >  1:435800/1‑150 (MQ=255)
                                                                                                                                   agcagGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGt                   <  1:3494333/150‑1 (MQ=255)
                                                                                                                                     cagGTTTTCTTCATTCAGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGTAc                 <  2:461420/150‑1 (MQ=255)
                                                                                                                                        gTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGTACCCa              <  1:3823430/150‑1 (MQ=255)
                                                                                                                                           ttcttcATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGTACCCAGcc           <  2:3965973/150‑1 (MQ=255)
                                                                                                                                              ttcATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGTACCCAGCcgcg        <  1:4569604/150‑1 (MQ=255)
                                                                                                                                                 aTTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGTACCCAGCCGCGACg     >  1:823519/1‑150 (MQ=255)
                                                                                                                                                   tCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGTACCCAGCCGCGACGTg   <  2:4622138/150‑1 (MQ=255)
                                                                                                                                                   tCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGTACCCAGCCGCGACGTg   <  2:3635286/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TCTGATCGTTAAACCACCAGCCGGAACCGAACTGCATTTTGCCCGGCATACCTTCGCCCTGGAAGTTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTACAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGCAACTTAGACAGCTCTTCCGCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGCTGTACCCAGCCGCGACGTGC  >  CP009273/3236942‑3237239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑