Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,376,534 C→A 19.3% R326S (CGC→AGC)  degS → serine endoprotease, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,376,5340CA19.3% 920.8 / 169.0 326R326S (CGC→AGC) degSserine endoprotease, periplasmic
Reads supporting (aligned to +/- strand):  ref base C (125/138);  new base A (25/38);  total (150/176)
Fisher's exact test for biased strand distribution p-value = 3.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 330 total aligned reads displayed.

AGATCAACTGCAAGGGATCGTGGTTAATGAAGTGTCACCTGACGGCCCGGCGGCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTA  >  CP009273/3376386‑3376683
                                                                                                                                                    |                                                                                                                                                     
  aTCAACTGCAAGGGATCGTGGTTAATGAAGTGTCACCTGACGGCCCGGCGGCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTAGcc                                                                                                                                                    <  1:3838156/150‑1 (MQ=255)
   tCAACTGCAAGGGATCGTGGTTAATGAAGTGTCACCTGACGGCCCGGCGGCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGccc                                                                                                                                                   >  1:1686975/1‑150 (MQ=255)
    cAACTGCAAGGGATCGTGGTTAATGAAGTGTCACCTGACGGCCCGGCGGCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCt                                                                                                                                                  >  2:4536823/1‑150 (MQ=255)
     aaCTGCAAGGGATCGTGGTTAATGAAGTGTCACCTGACGGCCCGGCGGCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTg                                                                                                                                                 <  2:1378412/150‑1 (MQ=255)
      aCTGCAAGGGATCGTGGTTAATGAAGTGTCACCTGACGGCCCGGCGGCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTAGCCCTgg                                                                                                                                                >  1:2183288/1‑150 (MQ=255)
         gCAAGGGATCGTGGTTAATGAAGTGTCACCTGACGGCCCGGCGGCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTc                                                                                                                                             <  2:116294/150‑1 (MQ=255)
         gCAAGGGATCGTGGTTAATGAAGTGTCACCTGACGGCCCGGCGGCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTc                                                                                                                                             <  1:4214270/150‑1 (MQ=255)
         gCAAGGGATCGTGGTTAATGAAGTGTCACCTGACGGCCCGGCGGCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTc                                                                                                                                             <  2:3298956/150‑1 (MQ=255)
          cAAGGGATCGTGGTTAATGAAGTGTCACCTGACGGCCCGGCGGCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCg                                                                                                                                            >  2:1999887/1‑150 (MQ=255)
          cAAGGGATCGTGGTTAATGAAGTGTCACCTGACGGCCCGGCGGCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTAGCCCTGGTTCg                                                                                                                                            >  1:4109360/1‑150 (MQ=255)
             gggATCGTGGTTAATGAAGTGTCACCTGACGGCCCGGCGGCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTg                                                                                                                                         <  1:433939/150‑1 (MQ=255)
              ggATCGTGGTTAATGAAGTGTCACCTGACGGCCCGGCGTCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGa                                                                                                                                        <  1:4656298/150‑1 (MQ=255)
                  cGTGGTTAATGAAGTGTCACCTGACGGCCCGGCGGCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATccc                                                                                                                                    <  1:1842060/150‑1 (MQ=255)
                  cGTGGTTAATGAAGTGTCACCTGACGGCCCGGCGGCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATccc                                                                                                                                    <  1:2921127/150‑1 (MQ=255)
                   gTGGTTAATGAAGTGTCACCTGACGGCCCGGCGGCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCt                                                                                                                                   >  2:917923/1‑150 (MQ=255)
                   gTGGTTAATGAAGTGTCACCTGACGGCCCGGCGGCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCt                                                                                                                                   <  2:399639/150‑1 (MQ=255)
                    tGGTTAATGAAGTGTCACCTGACGGCCCGGCGGCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTg                                                                                                                                  <  2:2945715/150‑1 (MQ=255)
                       ttAATGAAGTGTCACCTGACGGCCCGGCGGCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTgtag                                                                                                                               <  1:3409066/150‑1 (MQ=255)
                         aaTGAAGTGTCACCTGACGGCCCGGCGGCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTAGCCCTGGTTCGGTGATCCCTgtagta                                                                                                                             >  2:2667419/1‑150 (MQ=255)
                          aTGAAGTGTCACCTGACGGCCCGGCGGCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGtagtag                                                                                                                            <  2:3054029/150‑1 (MQ=255)
                          aTGAAGTGTCACCTGACGGCCCGGCGGCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGtagtag                                                                                                                            <  2:625093/150‑1 (MQ=255)
                          aTGAAGTGTCACCTGACGGCCCGGCGGCGAAGGCGGGTATTCAGGTCAACGCTCTGAGTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGgaggag                                                                                                                            >  2:4809778/1‑150 (MQ=255)
                            gAAGTGTCACCTGACGGCCCGGCGGCGAATGCGGGTATTCAAGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTg                                                                                                                          >  2:3705739/1‑150 (MQ=255)
                             aaGTGTCACCTGACGGCCCGGCGGCGAATGCGGGTATTCAGGTCAACGATCTGCTTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTAGCCCTGGTTCGGTGATCCCTGTAGTAGTGa                                                                                                                         <  1:431106/150‑1 (MQ=255)
                             aaGTGTCACCTGACGGCCCGGCGGCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTAGCCCTGGTTCGGTGATCCCTGTAGTAGTGa                                                                                                                         <  1:2862835/150‑1 (MQ=255)
                              aGTGTCACCTGACGGCCCGGCGGCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGAt                                                                                                                        >  2:2271683/1‑150 (MQ=255)
                              aGTGTCACCTGACGGCCCGGCGGCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTAGCCCTGGTTCGGTGATCCCTGTAGTAGTGAt                                                                                                                        <  1:249555/150‑1 (MQ=255)
                              aGTGTCACCTGACGGCCCGGCGGCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTAGCCCTGGTTCGGTGATCCCTGTAGTAGTGAt                                                                                                                        <  1:2910085/150‑1 (MQ=255)
                              aGTGTCACCTGACGGCCCAGCGGCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACAGGCCATCTCTGCTCGGGAGACGATGGATCAGGTGGCGGAAATTAGCCCTGGTTCGGTGATCCCTGTAGTAGTGAt                                                                                                                        >  1:4553669/1‑150 (MQ=255)
                                  tCACCTGACGGCCCGGCGGCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGt                                                                                                                    >  1:4786362/1‑150 (MQ=255)
                                  tCACCTGACGGCCCGGCGGCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTAGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGt                                                                                                                    >  2:2503460/1‑150 (MQ=255)
                                   cACCTGACGGCCCGGCGGCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGtg                                                                                                                   >  2:1785394/1‑150 (MQ=255)
                                   cACCTGACGGCCCGGCGGCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGtg                                                                                                                   >  2:1785167/1‑150 (MQ=255)
                                       tGACGGCCCGGCGGCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGtgatga                                                                                                               >  1:741235/1‑150 (MQ=255)
                                        gACGGCCCGGCGGCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTgatgat                                                                                                              >  2:745980/1‑150 (MQ=255)
                                         aCGGCCCGGCGGCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATa                                                                                                             >  1:3058009/1‑150 (MQ=255)
                                          cGGCCCGGCGGCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATaa                                                                                                            <  2:3683801/150‑1 (MQ=255)
                                          cGGCCCGGCGGCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATaa                                                                                                            >  2:1502404/1‑150 (MQ=255)
                                            gCCCGGCGGCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGc                                                                                                          <  2:2594809/150‑1 (MQ=255)
                                             cccGGCGGCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCa                                                                                                         >  2:597654/1‑150 (MQ=255)
                                              ccGGCGGCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAg                                                                                                        >  2:2635998/1‑150 (MQ=255)
                                              ccGGCGGCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAg                                                                                                        >  2:1526831/1‑150 (MQ=255)
                                                ggcggcGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGtt                                                                                                      <  1:1275437/150‑1 (MQ=255)
                                                ggcggcGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGtt                                                                                                      <  2:3163977/150‑1 (MQ=255)
                                                ggcggcGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGtt                                                                                                      <  2:4861669/150‑1 (MQ=255)
                                                 gcggcgAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTa                                                                                                     <  1:227497/150‑1 (MQ=255)
                                                  cggcgAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTaa                                                                                                    <  1:2558192/150‑1 (MQ=255)
                                                  cggcgAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTAGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTaa                                                                                                    <  1:1972259/150‑1 (MQ=255)
                                                  cggcgAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTAGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTaa                                                                                                    >  1:3269686/1‑150 (MQ=255)
                                                    gcgAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACg                                                                                                  <  1:4387521/150‑1 (MQ=255)
                                                           cGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTAGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAgg                                                                                           >  1:1496766/1‑150 (MQ=255)
                                                                  tCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCAt                                                                                    <  2:1643552/150‑1 (MQ=255)
                                                                   cAGGTCAACGATCTGATTATTTCGGTGGATAACAACCCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTAGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCAtt                                                                                   <  2:1814515/150‑1 (MQ=255)
                                                                   cAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTAGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCAtt                                                                                   <  2:4270042/150‑1 (MQ=255)
                                                                    aGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTc                                                                                  >  1:858047/1‑150 (MQ=255)
                                                                    aGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTAGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTc                                                                                  <  1:3856304/150‑1 (MQ=255)
                                                                     ggTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTAGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCa                                                                                 <  2:906421/150‑1 (MQ=255)
                                                                      gTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAg                                                                                <  1:1135532/150‑1 (MQ=255)
                                                                      gTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAg                                                                                <  2:1666668/150‑1 (MQ=255)
                                                                       tCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTAGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATCAGCAGTTAACGCTGCAGGTCACCATTCAgg                                                                               >  1:2442243/1‑150 (MQ=255)
                                                                         aaCGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTAGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGaa                                                                             >  1:4467959/1‑150 (MQ=255)
                                                                          aCGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAat                                                                            >  1:1334266/1‑150 (MQ=255)
                                                                             aTCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTAGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATc                                                                         >  2:73481/1‑150 (MQ=255)
                                                                               cTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCg                                                                       <  1:4438933/150‑1 (MQ=255)
                                                                                tGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCgg                                                                      <  2:3422778/150‑1 (MQ=255)
                                                                                tGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCgg                                                                      <  2:99352/150‑1 (MQ=255)
                                                                                 gATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTAGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGc                                                                     >  2:2292563/1‑150 (MQ=255)
                                                                                  attattTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCa                                                                    <  1:623201/150‑1 (MQ=255)
                                                                                  attattTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCa                                                                    >  1:2877132/1‑150 (MQ=255)
                                                                                    tattTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTAGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAAc                                                                  <  2:2183288/150‑1 (MQ=255)
                                                                                     attTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAAcc                                                                 >  1:2358704/1‑150 (MQ=255)
                                                                                        tCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAAt                                                              <  1:2616882/150‑1 (MQ=255)
                                                                                         cGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAAtt                                                             <  2:825089/150‑1 (MQ=255)
                                                                                          ggTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTTGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTa                                                            <  1:3123889/150‑1 (MQ=255)
                                                                                               aTAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCg                                                       <  1:951826/150‑1 (MQ=255)
                                                                                               aTAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCg                                                       <  1:4728454/150‑1 (MQ=255)
                                                                                                tAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTAGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGt                                                      >  2:488032/1‑150 (MQ=255)
                                                                                                 aaCAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTg                                                     >  1:2426987/1‑150 (MQ=255)
                                                                                                       ccGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTca                                               <  2:2439311/150‑1 (MQ=255)
                                                                                                         ggCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTcaaa                                             >  2:4657879/1‑150 (MQ=255)
                                                                                                         ggCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTcaaa                                             >  1:3755012/1‑150 (MQ=255)
                                                                                                         ggCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTcaaa                                             >  1:1626649/1‑150 (MQ=255)
                                                                                                         ggCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTcaaa                                             >  1:2669310/1‑150 (MQ=255)
                                                                                                            cATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTcaaaaca                                          <  2:4292273/150‑1 (MQ=255)
                                                                                                             aTCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTcaaaacaa                                         >  1:4800288/1‑150 (MQ=255)
                                                                                                             aTCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTAGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTcaaaacaa                                         >  1:2978420/1‑150 (MQ=255)
                                                                                                              tctcTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTcaaaacaaa                                        >  2:124873/1‑150 (MQ=255)
                                                                                                                tctgctctgGAGACGATGGATCAGGTGGCGGAAATTAGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACaaaaa                                      >  1:3502003/1‑150 (MQ=255)
                                                                                                                    ctctgGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCg                                  <  1:1017138/150‑1 (MQ=255)
                                                                                                                       tgGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAg                               >  1:2795639/1‑150 (MQ=255)
                                                                                                                             cGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCtgtg                         <  1:826049/150‑1 (MQ=255)
                                                                                                                               aTGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCt                       >  1:1090471/1‑150 (MQ=255)
                                                                                                                                tGGATCAGGTGGCGGAAATTAGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTc                      <  2:4109360/150‑1 (MQ=255)
                                                                                                                                 ggATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTcc                     <  2:144047/150‑1 (MQ=255)
                                                                                                                                  gATCAGGTGGCGGAAATTAGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCg                    <  2:3726647/150‑1 (MQ=255)
                                                                                                                                     cAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGtt                 >  1:1933266/1‑150 (MQ=255)
                                                                                                                                              gAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTtattat        <  1:1742237/150‑1 (MQ=255)
                                                                                                                                               aaaTTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATc       <  1:1194966/150‑1 (MQ=255)
                                                                                                                                               aaaTTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTGTATTATc       >  1:2035365/1‑150 (MQ=255)
                                                                                                                                                aaTTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATcc      <  2:2877132/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AGATCAACTGCAAGGGATCGTGGTTAATGAAGTGTCACCTGACGGCCCGGCGGCGAATGCGGGTATTCAGGTCAACGATCTGATTATTTCGGTGGATAACAAACCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTA  >  CP009273/3376386‑3376683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑