Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,406,591 C→T 30.8% T78T (ACG→ACA)  acrS ← acrAB operon transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,406,5910CT30.8% 304.7 / 185.3 201T78T (ACG→ACA) acrSacrAB operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base C (101/38);  new base T (42/20);  total (143/58)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

Only 100 of 203 total aligned reads displayed.

CTCCCTCGGCCAGCATCTCATCATTAAATTCACATTTGTGATATAAGATTTTCAGCAACGCCTGCTGGCGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTAGCGGCGTCGGCAATGTCGTTGAGCGTCGTCTTGCTTA  >  CP009273/3406443‑3406739
                                                                                                                                                    |                                                                                                                                                    
   ccTCGGCCAGCATCTCATCATTAAATTCACATTTGTGATATAAGATTTTCAGCAACGCCTGCTGGCGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCa                                                                                                                                                  >  2:1583552/1‑150 (MQ=255)
      cGGCCAGCATCTCATCATTAAATTCACATTTGTGATATAAGATTTTCAGCAACGCCTGCTGGCGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAgt                                                                                                                                               <  2:2168255/150‑1 (MQ=255)
       ggCCAGCATCTCATCATTAAATTCACATTTGTGATATAAGATTTTCAGCAACGCCTGCTGGCGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAgtg                                                                                                                                              >  2:102338/1‑150 (MQ=255)
         ccAGCATCTCATCATTAAATTCACATTTGTGATATAAGATTTTCAGCAACGCCTGCTGGCGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCTGTCAAGTGtt                                                                                                                                            <  2:335699/150‑1 (MQ=255)
          cAGCATCTCATCATTAAATTCACATTTGTGATATAAGATTTTCAGCAACGCCTGCTGGCGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTc                                                                                                                                           >  2:2361011/1‑150 (MQ=255)
           aGCATCTCATCATTAAATTCACATTTGTGATATAAGATTTTCAGCAACGCCTGCTGGCGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCTGTCAAGTGTTcc                                                                                                                                          >  2:463433/1‑150 (MQ=255)
           aGCATCTCATCATTAAATTCACATTTGTGATATAAGATTTTCAGCAACGCCTGCTGGCGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTcc                                                                                                                                          >  2:3676326/1‑150 (MQ=255)
             cATCTCATCATTAAATTCACATTTGTGATATAAGATTTTCAGCAACGCCTGCTGGCGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCTGTCAAGTGTTCCTg                                                                                                                                        <  2:4295005/150‑1 (MQ=255)
             cATCTCATCATTAAATTCACATTTGTGATATAAGATTTTCAGCAACGCCTGCTGGCGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTg                                                                                                                                        >  2:1389788/1‑150 (MQ=255)
                ctcATCATTAAATTCACATTTGTGATATAAGATTTTCAGCAACGCCTGCTGGCGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGAt                                                                                                                                     >  2:489063/1‑150 (MQ=255)
                  catcatTAAATTCACATTTGTGATATAAGATTTTCAGCAACGCCTGCTGGCGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTa                                                                                                                                   >  2:3151866/1‑150 (MQ=255)
                     catTAAATTCACATTTGTGATATAAGATTTTCAGCAACGCCTGCTGGCGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCTGTCAAGTGTTCCTGGATTAACt                                                                                                                                >  1:1373303/1‑150 (MQ=255)
                         aaaTTCACATTTGTGATATAAGATTTTCAGCAACGCCTGCTGGCGGGGACTTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCTGTCAAGTGTTCCTGGATTAACTCCCg                                                                                                                            >  2:3612190/1‑150 (MQ=255)
                         aaaTTCACATTTGTGATATAAGATTTTCAGCAACGCCTGCTGGCGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCTGTCAAGTGTTCCTGGATTAACTCCCg                                                                                                                            >  2:2008198/1‑150 (MQ=255)
                            ttCACATTTGTGATATAAGATTTTCAGCAACGCCTGCTGGCGGGGACTTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCTGTCAAGTGTTCCTGGATTAACTCCCGCaa                                                                                                                         >  2:3626649/1‑150 (MQ=255)
                             tCACATTTGTGATATAAGATTTTCAGCAACGCCTGCTGGCGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCTGTCAAGTGTTCCTGGATTAACTCCCGCAAt                                                                                                                        <  1:53621/150‑1 (MQ=255)
                                  tttGTGATATAAGATTTTCAGCAACGCCTGCTGGCGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAgg                                                                                                                   <  1:3082207/150‑1 (MQ=255)
                                   ttGTGATATAAGATTTTCAGCAACGCCTGCTGGCGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGc                                                                                                                  >  1:4411220/1‑150 (MQ=255)
                                    tgtgATATAAGATTTTCAGCAACGCCTGCTGGCGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCTGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCt                                                                                                                 >  2:2913500/1‑150 (MQ=255)
                                       gATATAAGATTTTCAGCAACGCCTGCTGGCGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGtt                                                                                                              <  2:193037/150‑1 (MQ=255)
                                         tataAGATTTTCAGCAACGCCTGCTGGCGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGc                                                                                                            <  1:1432601/150‑1 (MQ=255)
                                          ataAGATTTTCAGCAACGCCTGCTGGCGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCa                                                                                                           >  1:1596217/1‑150 (MQ=255)
                                                ttttCAGCAACGCCTGCTGGCGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCTGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACcaca                                                                                                     >  1:876683/1‑150 (MQ=255)
                                                ttttCAGCAACGCCTGCTGGCGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCTGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACcaca                                                                                                     >  1:1985266/1‑150 (MQ=255)
                                                   tCAGCAACGCCTGCTGGCGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCTGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACAtct                                                                                                  >  2:4813091/1‑150 (MQ=255)
                                                    cAGCAACGCCTGCTGGCGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACAtctc                                                                                                 >  1:1006381/1‑150 (MQ=255)
                                                    cAGCAACGCCTGCTGGCGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACAtctc                                                                                                 >  2:4422330/1‑150 (MQ=255)
                                                    cAGCAACGCCTGCTGGCGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACAtctc                                                                                                 >  1:1028722/1‑150 (MQ=255)
                                                     aGCAACGCCTGCTGGCGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCTGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCa                                                                                                >  1:558276/1‑150 (MQ=255)
                                                       cAACGCCTGCTGGCGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCTGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCAtt                                                                                              >  1:4631725/1‑150 (MQ=255)
                                                       cAACGCCTGCTGGCGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCTGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCAtt                                                                                              <  2:2462833/150‑1 (MQ=255)
                                                        aaCGCCTGCTGGCGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTa                                                                                             <  2:3161345/150‑1 (MQ=255)
                                                         aCGCCTGCTGGCGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCTGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTaa                                                                                            <  1:1931220/150‑1 (MQ=255)
                                                         aCGCCTGCTGGCGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTaa                                                                                            <  1:3603253/150‑1 (MQ=255)
                                                               gctgGCGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGtt                                                                                      >  2:1873286/1‑150 (MQ=255)
                                                               gctgGCGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGtt                                                                                      >  2:819681/1‑150 (MQ=255)
                                                                ctgGCGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTg                                                                                     <  2:2870241/150‑1 (MQ=255)
                                                                 tgGCGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCTGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGa                                                                                    <  1:3488721/150‑1 (MQ=255)
                                                                 tgGCGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGa                                                                                    <  2:1090552/150‑1 (MQ=255)
                                                                  ggCGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCTGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAg                                                                                   <  2:1539395/150‑1 (MQ=255)
                                                                    cGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTc                                                                                 >  2:535135/1‑150 (MQ=255)
                                                                    cGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTc                                                                                 >  2:1278779/1‑150 (MQ=255)
                                                                    cGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTc                                                                                 <  1:2649417/150‑1 (MQ=255)
                                                                     ggggAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCt                                                                                >  2:256871/1‑150 (MQ=255)
                                                                        gAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGt                                                                             >  2:3070020/1‑150 (MQ=255)
                                                                               ggCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGATGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGaa                                                                      >  1:2861310/1‑150 (MQ=255)
                                                                                gCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCTGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAg                                                                     <  2:1325037/150‑1 (MQ=255)
                                                                                 cAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCTGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGt                                                                    >  1:3394050/1‑150 (MQ=255)
                                                                                    tatatTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGcc                                                                 >  2:1811192/1‑150 (MQ=255)
                                                                                      tatTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAg                                                               <  1:3808745/150‑1 (MQ=255)
                                                                                          gCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCTGTCTAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGa                                                           <  1:1592025/150‑1 (MQ=255)
                                                                                           cAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCTGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGAt                                                          <  1:955707/150‑1 (MQ=255)
                                                                                           cAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGAt                                                          <  1:2152070/150‑1 (MQ=255)
                                                                                               ccGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAgcg                                                      >  2:676636/1‑150 (MQ=255)
                                                                                                cGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAgcgc                                                     >  2:4763463/1‑150 (MQ=255)
                                                                                                      tcaatTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCAcgcg                                               <  1:1836671/150‑1 (MQ=255)
                                                                                                       caatTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGt                                              >  1:4073868/1‑150 (MQ=255)
                                                                                                         atTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCTGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTaa                                            >  1:126959/1‑150 (MQ=255)
                                                                                                         atTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCTGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTaa                                            >  1:2556750/1‑150 (MQ=255)
                                                                                                            tttCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGt                                         >  2:4093325/1‑150 (MQ=255)
                                                                                                              tCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTa                                       >  2:3387425/1‑150 (MQ=255)
                                                                                                              tCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTa                                       >  2:2627998/1‑150 (MQ=255)
                                                                                                              tCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTa                                       >  2:2630637/1‑150 (MQ=255)
                                                                                                               cACGCACTTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTAg                                      >  1:701191/1‑150 (MQ=255)
                                                                                                                 cGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTAgcg                                    >  2:3372930/1‑150 (MQ=255)
                                                                                                                  gCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTAgcgg                                   <  2:3473711/150‑1 (MQ=255)
                                                                                                                  gCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTAgcgg                                   >  2:425601/1‑150 (MQ=255)
                                                                                                                   cAATTGTTGAAACGGGTCATGCTCTAATCCAGCTGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTAgcggc                                  >  2:4144050/1‑150 (MQ=255)
                                                                                                                   cAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACAACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTAgcggc                                  >  1:4864489/1‑150 (MQ=255)
                                                                                                                    aaTTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTAgcggcg                                 >  1:3449839/1‑150 (MQ=255)
                                                                                                                     aTTGTTGAAACGGGTCATGCTCTAATCCAGCTGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTAGCGGCGt                                >  1:1063896/1‑150 (MQ=255)
                                                                                                                       tgttgAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTAGCGGCGTCg                              >  1:305019/1‑150 (MQ=255)
                                                                                                                         ttgAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTAGCGGCGTCGGc                            >  1:3535263/1‑150 (MQ=255)
                                                                                                                          tgAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTAGCGGCGTCGGCa                           >  1:4639207/1‑150 (MQ=255)
                                                                                                                          tgAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTAGCGGCGTCGGCa                           >  1:2433321/1‑150 (MQ=255)
                                                                                                                          tgAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTAGCGGCGTCGGCa                           <  2:3395889/150‑1 (MQ=255)
                                                                                                                          tgAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTAGCGGCGTCGGCa                           <  1:2833041/150‑1 (MQ=255)
                                                                                                                           gAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTAGCGGCGTCGGCaa                          >  2:3962793/1‑150 (MQ=255)
                                                                                                                            aaaCGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTAGCGGCGTCGGCAAt                         >  1:3139448/1‑150 (MQ=255)
                                                                                                                            aaaCGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTAGCGGCGTCGGCAAt                         >  1:3956668/1‑150 (MQ=255)
                                                                                                                               cGGGTCATGCTCTAATCCAGCTGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTAGCGGCGTCGGCAATGTc                      >  2:274019/1‑150 (MQ=255)
                                                                                                                                gggTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTAGCGGCGTCGGCAATGTCg                     <  1:3864943/150‑1 (MQ=255)
                                                                                                                                   tCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTAGCGGCGTCGGCAATGTCGTTg                  <  1:2627998/150‑1 (MQ=255)
                                                                                                                                    cATGCTCTACTCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTAGCGGCGTCGGCAATGTCGTTGa                 <  2:610906/150‑1 (MQ=255)
                                                                                                                                    cATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTTAGTCTTGTTTTCTAAGTGCCAGTAGATAGAGCCACGCGTAACGATAGATGCGTCGGCAATGTCGATGa                 >  2:3672528/1‑150 (MQ=255)
                                                                                                                                    cATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTAGCGGCGTCGGCAATGTCGTTGa                 >  2:4129533/1‑150 (MQ=255)
                                                                                                                                     aTGCTCTAATCCAGCTGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTAGCGGCGTCGGCAATGTCGTTGAg                >  2:3832953/1‑150 (MQ=255)
                                                                                                                                     aTGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTAGCGGCGTCGGCAATGTCGTTGAg                <  1:102300/150‑1 (MQ=255)
                                                                                                                                      tGCTCTAATCCAGCTGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTAGCGGCGTCGGCAATGTCGTTGAGc               >  2:4103215/1‑150 (MQ=255)
                                                                                                                                      tGCTCTAATCCAGCTGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTAGCGGCGTCGGCAATGTCGTTGAGc               >  2:4702411/1‑150 (MQ=255)
                                                                                                                                      tGCTCTAATCCAGCTGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTAGCGGCGTCGGCAATGTCGTTGAGc               >  2:2251801/1‑150 (MQ=255)
                                                                                                                                             aTCCAGCTGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTAGCGGCGTCGGCAATGTCGTTGAGCGTCGTCt        >  1:4752292/1‑150 (MQ=255)
                                                                                                                                             aTCCAGCTGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTAGCGGCGTCGGCAATGTCGTTGAGCGTCGTCt        >  1:4156078/1‑150 (MQ=255)
                                                                                                                                                cAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTAGCGGCGTCGGCAATGTCGTTGAGCGTCGTCTTGc     >  2:832597/1‑150 (MQ=255)
                                                                                                                                                 aGCTGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTAGCGGCGTCGGCAATGTCGTTGAGCGTCGTCTTGCt    >  2:2556285/1‑150 (MQ=255)
                                                                                                                                                 aGCTGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTAGCGGCGTCGGCAATGTCGTTGAGCGTCGTCTTGCt    >  1:451830/1‑150 (MQ=255)
                                                                                                                                                 aGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTAGCGGCGTCGGCAATGTCGTTGAGCGTCGTCTTGCt    >  2:4866732/1‑150 (MQ=255)
                                                                                                                                                  gCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTAGCGGCGTCGGCAATGTCGTTGAGCGTCGTCTTGCtt   >  1:4691048/1‑150 (MQ=255)
                                                                                                                                                   ccGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTAGCGGCGTCGGCAATGTCGTTGAGCGTCGTCTTGCTTa  >  1:2186113/1‑150 (MQ=255)
                                                                                                                                                   ccGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTAGCGGCGTCGGCAATGTCGTTGAGCGTCGTCTTGCTTa  <  1:210892/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CTCCCTCGGCCAGCATCTCATCATTAAATTCACATTTGTGATATAAGATTTTCAGCAACGCCTGCTGGCGGGGAATTTTGGCAATATATTGCAAGCCGACAATCAATTTTTCACGCAATTGTTGAAACGGGTCATGCTCTAATCCAGCCGTCAAGTGTTCCTGGATTAACTCCCGCAATGAAGGCTGTTGCAACCACATCTCATTAAACAGTTGAGTCTTGTTTTCGAAGTGCCAGTAGATAGCGCCACGCGTAACGTTAGCGGCGTCGGCAATGTCGTTGAGCGTCGTCTTGCTTA  >  CP009273/3406443‑3406739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑