Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,440,151 G→T 68.9% I31I (ATC→ATA)  rpsN ← 30S ribosomal subunit protein S14

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,440,1510GT68.9% 316.5 / 286.9 257I31I (ATC→ATA) rpsN30S ribosomal subunit protein S14
Reads supporting (aligned to +/- strand):  ref base G (25/55);  new base T (75/102);  total (100/157)
Fisher's exact test for biased strand distribution p-value = 9.88e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01

Only 100 of 264 total aligned reads displayed.

GGCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCG  >  CP009273/3440003‑3440297
                                                                                                                                                    |                                                                                                                                                  
ggCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAgaga                                                                                                                                                   >  2:2890602/1‑150 (MQ=255)
   tCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAtatga                                                                                                                                                >  1:1859235/1‑150 (MQ=255)
     aaCCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATc                                                                                                                                              <  1:936809/150‑1 (MQ=255)
     aaCCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATc                                                                                                                                              >  2:2532227/1‑150 (MQ=255)
       cccGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGc                                                                                                                                            >  1:2149777/1‑150 (MQ=255)
        ccGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCt                                                                                                                                           <  1:984384/150‑1 (MQ=255)
          gAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCttt                                                                                                                                         >  1:4598173/1‑150 (MQ=255)
          gAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCttt                                                                                                                                         <  1:2452384/150‑1 (MQ=255)
           aaCTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTttc                                                                                                                                        <  1:1970120/150‑1 (MQ=255)
              ttCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTttcagt                                                                                                                                     >  1:897191/1‑150 (MQ=255)
               tCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTttcagtt                                                                                                                                    <  2:781168/150‑1 (MQ=255)
                ccGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTttcagttc                                                                                                                                   <  1:1250252/150‑1 (MQ=255)
                   cAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAgc                                                                                                                                >  1:4077489/1‑150 (MQ=255)
                    aGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAgcg                                                                                                                               <  2:2664031/150‑1 (MQ=255)
                    aGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAgcg                                                                                                                               <  2:234376/150‑1 (MQ=255)
                      gAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGcgcg                                                                                                                             >  2:1268809/1‑150 (MQ=255)
                       aaaCCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGt                                                                                                                            >  1:486280/1‑150 (MQ=255)
                       aaaCCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGt                                                                                                                            >  1:3001680/1‑150 (MQ=255)
                         aCCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGttt                                                                                                                          <  1:2289602/150‑1 (MQ=255)
                         aCCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGttt                                                                                                                          >  1:3250668/1‑150 (MQ=255)
                          ccATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTc                                                                                                                         <  2:2729967/150‑1 (MQ=255)
                          ccATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTc                                                                                                                         <  2:3072344/150‑1 (MQ=255)
                          ccATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTc                                                                                                                         <  2:3989383/150‑1 (MQ=255)
                           cATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTcg                                                                                                                        <  1:108802/150‑1 (MQ=255)
                           cATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTcg                                                                                                                        <  2:1892790/150‑1 (MQ=255)
                           cATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTcg                                                                                                                        <  2:705264/150‑1 (MQ=255)
                             tGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTcgcg                                                                                                                      <  2:4165178/150‑1 (MQ=255)
                                ggACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAg                                                                                                                   <  2:4117199/150‑1 (MQ=255)
                                   cgacCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTAt                                                                                                                <  1:4509663/150‑1 (MQ=255)
                                   cgacCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTAt                                                                                                                <  1:2056977/150‑1 (MQ=255)
                                        tGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATc                                                                                                           >  1:319978/1‑150 (MQ=255)
                                        tGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATc                                                                                                           <  1:3718378/150‑1 (MQ=255)
                                          tttGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAg                                                                                                         <  1:2597872/150‑1 (MQ=255)
                                           ttGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGc                                                                                                        <  1:927113/150‑1 (MQ=255)
                                            tGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCt                                                                                                       <  1:2051449/150‑1 (MQ=255)
                                              aCGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTaa                                                                                                     >  2:2085468/1‑150 (MQ=255)
                                                  cAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCt                                                                                                 <  2:3164809/150‑1 (MQ=255)
                                                   aGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTa                                                                                                <  2:1775776/150‑1 (MQ=255)
                                                       gTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTAcgcg                                                                                            <  2:4273934/150‑1 (MQ=255)
                                                         tACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGtt                                                                                          <  1:2075028/150‑1 (MQ=255)
                                                         tACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGtt                                                                                          <  1:4652026/150‑1 (MQ=255)
                                                          aCGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGttt                                                                                         >  1:2434228/1‑150 (MQ=255)
                                                           cGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGtttt                                                                                        <  2:3203272/150‑1 (MQ=255)
                                                            gCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTa                                                                                       <  2:3876265/150‑1 (MQ=255)
                                                              tGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACt                                                                                     <  1:1533911/150‑1 (MQ=255)
                                                              tGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACt                                                                                     <  1:1694366/150‑1 (MQ=255)
                                                              tGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACt                                                                                     >  2:3740974/1‑150 (MQ=255)
                                                              tGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACt                                                                                     <  2:903038/150‑1 (MQ=255)
                                                               gACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACtt                                                                                    <  1:4449859/150‑1 (MQ=255)
                                                               gACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACtt                                                                                    <  1:1511587/150‑1 (MQ=255)
                                                                  gagaCGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTcgc                                                                                 <  1:2647352/150‑1 (MQ=255)
                                                                    gaCGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGt                                                                               <  1:73609/150‑1 (MQ=255)
                                                                     aCGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTg                                                                              <  2:4714969/150‑1 (MQ=255)
                                                                      cGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGGGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGc                                                                             >  2:1324481/1‑150 (MQ=255)
                                                                       gggCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCt                                                                            <  2:2134467/150‑1 (MQ=255)
                                                                       gggCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCt                                                                            >  1:1037151/1‑150 (MQ=255)
                                                                       gggCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCt                                                                            >  2:2545971/1‑150 (MQ=255)
                                                                          cTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTc                                                                         >  2:4649372/1‑150 (MQ=255)
                                                                          cTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTc                                                                         <  2:4257534/150‑1 (MQ=255)
                                                                          cTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTc                                                                         <  2:3782077/150‑1 (MQ=255)
                                                                           tGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCa                                                                        >  1:2724943/1‑150 (MQ=255)
                                                                              aaTCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCattg                                                                     <  1:517720/150‑1 (MQ=255)
                                                                                tCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCattgat                                                                   >  1:3440426/1‑150 (MQ=255)
                                                                                tCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCattgat                                                                   <  1:608179/150‑1 (MQ=255)
                                                                                      ggCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTa                                                             >  1:2965618/1‑150 (MQ=255)
                                                                                       gCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAg                                                            <  2:174215/150‑1 (MQ=255)
                                                                                         agagTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGcc                                                          <  2:3712862/150‑1 (MQ=255)
                                                                                         agagTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGcc                                                          <  1:4673808/150‑1 (MQ=255)
                                                                                            gTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCAtt                                                       <  2:53905/150‑1 (MQ=255)
                                                                                             tCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCAttt                                                      <  2:4613149/150‑1 (MQ=255)
                                                                                             tCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCAttt                                                      <  1:1152539/150‑1 (MQ=255)
                                                                                               tGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAg                                                    >  2:4761280/1‑150 (MQ=255)
                                                                                               tGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAg                                                    >  2:1527648/1‑150 (MQ=255)
                                                                                                gCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGt                                                   <  2:231758/150‑1 (MQ=255)
                                                                                                 cAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTa                                                  >  1:660240/1‑150 (MQ=255)
                                                                                                  aGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTaa                                                 >  2:436462/1‑150 (MQ=255)
                                                                                                   gCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAAc                                                <  1:336940/150‑1 (MQ=255)
                                                                                                    cTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAAcc                                               <  1:1174636/150‑1 (MQ=255)
                                                                                                    cTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAAcc                                               <  1:3633908/150‑1 (MQ=255)
                                                                                                    cTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCCTTTAGTAAcc                                               >  2:3358497/1‑150 (MQ=255)
                                                                                                    cTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAAcc                                               <  2:4347465/150‑1 (MQ=255)
                                                                                                           aCAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCtt                                        >  1:277576/1‑150 (MQ=255)
                                                                                                             aGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTAc                                      >  1:3425828/1‑150 (MQ=255)
                                                                                                               cGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACtt                                    >  1:4732068/1‑150 (MQ=255)
                                                                                                               cGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACtt                                    <  1:1780912/150‑1 (MQ=255)
                                                                                                               cGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACtt                                    <  1:1780958/150‑1 (MQ=255)
                                                                                                                gTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTg                                   >  1:4366685/1‑150 (MQ=255)
                                                                                                                gTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTg                                   >  1:3821636/1‑150 (MQ=255)
                                                                                                                      aCGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAAc                             >  1:430911/1‑150 (MQ=255)
                                                                                                                          tCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGa                         <  2:67280/150‑1 (MQ=255)
                                                                                                                             tcgtcgGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGt                      >  2:3660067/1‑150 (MQ=255)
                                                                                                                             tcgtcgGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCAGTTAGTAACCCTACCTTACTTGCGGACCGGGAAGt                      >  2:4434494/1‑150 (MQ=255)
                                                                                                                               gtcgGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCa                    <  1:2674248/150‑1 (MQ=255)
                                                                                                                               gtcgGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCa                    <  2:3530565/150‑1 (MQ=255)
                                                                                                                                tcgGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCaa                   <  2:929268/150‑1 (MQ=255)
                                                                                                                                  ggAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAg                 >  2:1810221/1‑150 (MQ=255)
                                                                                                                                    aaGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGc               <  1:548044/150‑1 (MQ=255)
                                                                                                                                       cGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGcagc            >  2:906942/1‑150 (MQ=255)
                                                                                                                                        gTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGcagcc           >  2:3156975/1‑150 (MQ=255)
                                                                                                                                            acaTCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCcagca       <  1:2890576/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GGCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCG  >  CP009273/3440003‑3440297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑