BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,522,558	G→T	26.1%	A177A (GCG→GCT)	yrfG →	GMP/IMP nucleotidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,522,558	0	G	T	26.1%	642.4 / 210.9	280	A177A (GCG→GCT)	yrfG	GMP/IMP nucleotidase
Reads supporting (aligned to +/- strand): ref base G (115/92); new base T (36/37); total (151/129)
Fisher's exact test for biased strand distribution p-value = 4.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 295 total aligned reads displayed.

AGCATACCGGTCTGGACGCACACCTTGATTTATTACTTTCCACCCACACATTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGCCATCCGTCACTGAATGACTACCGCCGCCTGATCCCCTCGCTAATGTGAAGGAGACGCCA  >  CP009273/3522410‑3522707

 gCATACCGGTCTGGACGCACACCTTGATTTATTACTTTCCACCCACACATTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGAt                                                                                                                                                     <  1:1024287/150‑1 (MQ=255)

    tACCGGTCTGGACGCACACCTTGATTTATTACTTTCCACCCACACAGTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCTATtct                                                                                                                                                  >  2:3378152/1‑150 (MQ=255)

      ccGGTCTGGACGCACACCTTGATTTATTACTTTCCACCCACACATTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCg                                                                                                                                                <  2:2812153/150‑1 (MQ=255)

        ggTCTGGACGCACACCTTGATTTATTACTTTCCACCCACACATTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGAt                                                                                                                                              >  2:2558902/1‑150 (MQ=255)

         gTCTGGACGCACACCTTGATTTATTACTTTCCACCCACACATTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGAtg                                                                                                                                             <  2:360423/150‑1 (MQ=255)

            tGGACGCACACCTTGATTTATTACTTTCCACCCACACATTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGAtgctg                                                                                                                                          <  2:4589929/150‑1 (MQ=255)

              gACGCACACCTTGATTTATTACTTTCCACCCACACATTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGcc                                                                                                                                        <  1:70164/150‑1 (MQ=255)

               aCGCACACCTTGATTTATTACTTTCCACCCACACATTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCcg                                                                                                                                       <  2:2336707/150‑1 (MQ=255)

                 gCACACCTTGATTTATTACTTTCCACCCACACATTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCTATTCTCGATGCTGCcgcg                                                                                                                                     >  1:3558987/1‑150 (MQ=255)

                  cacaCCTTGATTTATTACTTTCCACCCACACATTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCTATTCTCGATGCTGCCgcgc                                                                                                                                    >  2:4828328/1‑150 (MQ=255)

                    cacCTTGATTTATTACTTTCCACCCACACATTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCTATTCTCGATGCTGCCGCGCaa                                                                                                                                  >  1:2705821/1‑150 (MQ=255)

                     acCTTGATTTATTACTTTCCACCCACACATTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAAt                                                                                                                                 >  1:2604355/1‑150 (MQ=255)

                         tGATTTATTACTTTCCACCCACACATTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTgg                                                                                                                             >  1:3595307/1‑150 (MQ=255)

                          gATTTATTACTTTCCACCCACACATTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGt                                                                                                                            <  1:1099515/150‑1 (MQ=255)

                            tttATTACTTTCCACCCACACATTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTAt                                                                                                                          <  2:1548183/150‑1 (MQ=255)

                            tttATTACTTTCCACCCACACATGTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTAt                                                                                                                          <  2:549918/150‑1 (MQ=255)

                             ttattaCTTTCCACCCACACATTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCTATTCTCGATGCTGCCGCGCAATTTGGTAtt                                                                                                                         <  2:2492665/150‑1 (MQ=255)

                               attaCTTTCCACCCACACATTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCg                                                                                                                       >  1:1227443/1‑150 (MQ=255)

                                 taCTTTCCACCCACACATTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGtt                                                                                                                     >  2:3344043/1‑150 (MQ=255)

                                 taCTTTCCACCCACACATTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGtt                                                                                                                     <  1:3791653/150‑1 (MQ=255)

                                  aCTTTCCACCCACACATTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCTATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTa                                                                                                                    >  1:2845171/1‑150 (MQ=255)

                                  aCTTTCCACCCACACATTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTa                                                                                                                    >  1:3350208/1‑150 (MQ=255)

                                   cTTTCCACCCACACATTGGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCGGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTAc                                                                                                                   <  2:1465535/150‑1 (MQ=255)

                                    tttCCACCCACACATTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACt                                                                                                                  <  2:3794195/150‑1 (MQ=255)

                                     ttCCACCCACACATTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTg                                                                                                                 >  2:3263049/1‑150 (MQ=255)

                                      tCCACCCACACATTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGc                                                                                                                >  2:3947908/1‑150 (MQ=255)

                                       ccacccacACATTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCTATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGcc                                                                                                               >  2:496201/1‑150 (MQ=255)

                                        cacccacACATTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCTATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCt                                                                                                              >  2:2899835/1‑150 (MQ=255)

                                        cacccacACATTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCt                                                                                                              >  1:3208086/1‑150 (MQ=255)

                                          cccacACATTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCg                                                                                                            >  1:2207565/1‑150 (MQ=255)

                                            cacacaTTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGc                                                                                                          <  2:1874182/150‑1 (MQ=255)

                                             acacaTTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTCCGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCg                                                                                                         >  2:678208/1‑150 (MQ=255)

                                               acaTTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCTATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTg                                                                                                       >  1:3317051/1‑150 (MQ=255)

                                               acaTTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTg                                                                                                       >  2:3211772/1‑150 (MQ=255)

                                               acaTTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTg                                                                                                       >  1:1359065/1‑150 (MQ=255)

                                               acaTTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTg                                                                                                       >  2:2030912/1‑150 (MQ=255)

                                                caTTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGa                                                                                                      <  1:4363836/150‑1 (MQ=255)

                                                 aTTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCTATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGAc                                                                                                     >  2:1357787/1‑150 (MQ=255)

                                                  tttGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCTATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACt                                                                                                    <  2:4007359/150‑1 (MQ=255)

                                                     ggTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCTATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAAt                                                                                                 <  2:3297358/150‑1 (MQ=255)

                                                     ggTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAAt                                                                                                 <  2:128928/150‑1 (MQ=255)

                                                     ggTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAAt                                                                                                 >  1:1167794/1‑150 (MQ=255)

                                                      gTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATc                                                                                                <  2:3089364/150‑1 (MQ=255)

                                                       ttATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCTATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATcc                                                                                               >  2:4109923/1‑150 (MQ=255)

                                                        tATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCt                                                                                              <  1:4047336/150‑1 (MQ=255)

                                                        tATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCt                                                                                              <  2:4081867/150‑1 (MQ=255)

                                                            cGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGAtt                                                                                          >  1:608329/1‑150 (MQ=255)

                                                               aaGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCTATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCg                                                                                       >  1:2350321/1‑150 (MQ=255)

                                                               aaGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCg                                                                                       >  1:4187763/1‑150 (MQ=255)

                                                               aaGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCg                                                                                       >  1:3309621/1‑150 (MQ=255)

                                                               aaGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCg                                                                                       >  1:837645/1‑150 (MQ=255)

                                                                  agGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGa                                                                                    >  1:3767127/1‑150 (MQ=255)

                                                                   ggATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGAt                                                                                   <  2:3010350/150‑1 (MQ=255)

                                                                     aTCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTg                                                                                 >  2:4539755/1‑150 (MQ=255)

                                                                        aGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCg                                                                              >  2:2114283/1‑150 (MQ=255)

                                                                              tATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAAc                                                                        <  1:385145/150‑1 (MQ=255)

                                                                                tGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAg                                                                      <  2:3848519/150‑1 (MQ=255)

                                                                                  gCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCTATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTa                                                                    <  2:4272184/150‑1 (MQ=255)

                                                                                    aTGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATc                                                                  >  2:1030251/1‑150 (MQ=255)

                                                                                     tGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGGTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCa                                                                 <  2:3450383/150‑1 (MQ=255)

                                                                                       cGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAAc                                                               >  1:3032826/1‑150 (MQ=255)

                                                                                          tGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGcc                                                            <  1:3119496/150‑1 (MQ=255)

                                                                                           ggCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGCCa                                                           <  1:4400768/150‑1 (MQ=255)

                                                                                           ggCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGCCa                                                           >  2:1811479/1‑150 (MQ=255)

                                                                                                aaGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCTATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGCCATCCGt                                                      >  1:1582958/1‑150 (MQ=255)

                                                                                                aaGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCTATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGCCATCCGt                                                      <  1:2899874/150‑1 (MQ=255)

                                                                                                 aGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGCCATCCGTc                                                     >  2:2467146/1‑150 (MQ=255)

                                                                                                     aCGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGCCATCCGTCACTg                                                 >  2:3262278/1‑150 (MQ=255)

                                                                                                      cGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCTATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGCCATCCGTCACTGa                                                >  2:4704464/1‑150 (MQ=255)

                                                                                                      cGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGCCATCCGTCACTGa                                                >  1:4703471/1‑150 (MQ=255)

                                                                                                       gggTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGCCATCCGTCACTGaa                                               >  2:3600527/1‑150 (MQ=255)

                                                                                                          tCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGCCATCCGTCACTGAATGa                                            <  2:174954/150‑1 (MQ=255)

                                                                                                           ctgaaagctgaaagAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGCCATCCGTCACTGAATGAc                                           >  2:2269358/1‑150 (MQ=255)

                                                                                                            tgaaagctgaaagAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGCCATCCGTCACTGAATGACt                                          <  1:4721007/150‑1 (MQ=255)

                                                                                                            tgaaagctgaaagAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGCCATCCGTCACTGAATGACt                                          <  1:39620/150‑1 (MQ=255)

                                                                                                               aagctgaaagAACGCTGTTTATTGATGACAGCGAAGCTATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGCCATCCGTCACTGAATGACTAcc                                       <  2:2845171/150‑1 (MQ=255)

                                                                                                                agctgaaagAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGCCATCCGTCACTGAATGACTAccg                                      >  2:1190334/1‑150 (MQ=255)

                                                                                                                agctgaaagAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGCCATCCGTCACTGAATGACTAccg                                      <  2:3040236/150‑1 (MQ=255)

                                                                                                                  ctgaaagAACGCTGTTTATTGATGACAGCGAAGCTATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGCCATCCGTCACTGAATGACTAccgcc                                    <  1:2496903/150‑1 (MQ=255)

                                                                                                                  ctgaaagAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGCCATCCGTCACTGAATGACTAccgcc                                    >  2:932154/1‑150 (MQ=255)

                                                                                                                   tgaaagAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGCCATCCGTCACTGAATGACTAccgccg                                   >  1:1509212/1‑150 (MQ=255)

                                                                                                                     aaagAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGCCATCCGTCACTGAATGACTACCgccgcc                                 >  1:4536605/1‑150 (MQ=255)

                                                                                                                      aagAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGCCATCCGTCACTGAATGACTACCGCCGCCt                                <  1:1812099/150‑1 (MQ=255)

                                                                                                                         aaCGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGCCATCCGTCACTGAATGACTACCGCCGCCTGAt                             >  1:2467592/1‑150 (MQ=255)

                                                                                                                            gCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGCCATCCGTCACTGAATGACTACCGCCGCCTGATccc                          <  2:1649105/150‑1 (MQ=255)

                                                                                                                               gTTTATTGATGACAGCGAAGCGATGCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGCCATCCGTCACTGAATGACTACCGCCGCCTGATCCCCTc                       <  2:2796019/150‑1 (MQ=255)

                                                                                                                                tttATTGATGACAGCGAAGCTATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGCCATCCGTCACTGAATGACTACCGCCGCCTGATCCCCTCg                      <  1:1953761/150‑1 (MQ=255)

                                                                                                                                      gatgaCAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGCCATCCGTCACTGAATGACTACCGCCGCCTGATCCCCTCGCTAAtg                <  1:3490983/150‑1 (MQ=255)

                                                                                                                                      gatgaCAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGCCATCCGTCACTGAATGACTACCGCCGCCTGATCCCCTCGCTAAtg                <  2:4187763/150‑1 (MQ=255)

                                                                                                                                      gatgaCAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGCCATCCGTCACTGAATGACTACCGCCGCCTGATCCCCTCGCTAAtg                <  2:837645/150‑1 (MQ=255)

                                                                                                                                       atgaCAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGCCATCCGTCACTGAATGACTACCGCCGCCTGATCCCCTCGCTAAtgt               >  1:777725/1‑150 (MQ=255)

                                                                                                                                        tgaCAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGCCATCCGTCACTGAATGACTACCGCCGCCTGATCCCCTCGCTAAtgtg              <  1:506534/150‑1 (MQ=255)

                                                                                                                                         gaCAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGCCATCCGTCACTGAATGACTACCGCCGCCTGATCCCCTCGCTAATGTGa             >  2:4159335/1‑150 (MQ=255)

                                                                                                                                           cAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGCCATCCGTCACTGAATGACTACCGCCGCCTGATCCCCTCGCTAATGTGAAg           >  1:48059/1‑150 (MQ=255)

                                                                                                                                            agcgaagctaTTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGCCATCCGTCACTGAATGACTACCGCCGCCTGATCCCCTCGCTAATGTGAAgg          >  1:2062452/1‑150 (MQ=255)

                                                                                                                                            agcgaagcgaTTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGCCATCCGTCACTGAATGACTACCGCCGCCTGATCCCCTCGCTAATGTGAAgg          <  1:3011555/150‑1 (MQ=255)

                                                                                                                                            agcgaagcgaTTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGCCATCCGTCACTGAATGACTACCGCCGCCTGATCCCCTCGCTAATGTGAAgg          >  1:1495154/1‑150 (MQ=255)

                                                                                                                                              cgaagcgaTTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGCCATCCGTCACTGAATGACTACCGCCGCCTGATCCCCTCGCTAATGTGAAGgag        >  1:1962857/1‑150 (MQ=255)

                                                                                                                                               gaagcgaTTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGCCATCCGTCACTGAATGACTACCGCCGCCTGATCCCCTCGCTCATGTGAAGgaga       >  1:1484812/1‑150 (MQ=255)

                                                                                                                                                    gaTTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGCCATCCGTCACTGAATGACTACCGCCGCCTGATCCCCTCGCTAATGTGAAGGAGACGCCa  <  1:905604/150‑1 (MQ=255)

AGCATACCGGTCTGGACGCACACCTTGATTTATTACTTTCCACCCACACATTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTACGGGTCTGAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTATTCGTTACTGCCTCGGCGTGACTAATCCTGATTCCGGGATTGCCGAGAAACAGTATCAACGCCATCCGTCACTGAATGACTACCGCCGCCTGATCCCCTCGCTAATGTGAAGGAGACGCCA  >  CP009273/3522410‑3522707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`