Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 278,630 G→T 20.1% A214A (GCG→GCT)  yagE → 2‑keto‑3‑deoxy gluconate (KDG) aldolase; CP4‑6 prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273278,6300GT20.1% 920.3 / 178.9 347A214A (GCG→GCT) yagE2‑keto‑3‑deoxy gluconate (KDG) aldolase; CP4‑6 prophage
Reads supporting (aligned to +/- strand):  ref base G (153/123);  new base T (36/35);  total (189/158)
Fisher's exact test for biased strand distribution p-value = 5.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 355 total aligned reads displayed.

GGCATCAAAGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGATTAAAGA  >  CP009273/278481‑278779
                                                                                                                                                     |                                                                                                                                                     
  cATCAAAGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAg                                                                                                                                                     >  1:2331447/1‑150 (MQ=255)
  cATCAAAGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGCTGCCCATCCTCACTTCACCGAGCTCTGCGGCTACGACGAACAGCTGTTCAAAACCCAGCTGCTCGCCGGCGACGCGGCGATATCGGCGAg                                                                                                                                                     >  1:1780810/1‑150 (MQ=255)
   aTCAAAGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGc                                                                                                                                                    >  1:3023460/1‑150 (MQ=255)
   aTCAAAGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGc                                                                                                                                                    >  2:47670/1‑150 (MQ=255)
   aTCAAAGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGc                                                                                                                                                    >  2:4110401/1‑150 (MQ=255)
      aaaGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGc                                                                                                                                                 <  2:3869842/150‑1 (MQ=255)
         gACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCTAGCGGCAAc                                                                                                                                              >  1:4259346/1‑150 (MQ=255)
          acacCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACt                                                                                                                                             <  2:3013451/150‑1 (MQ=255)
           cacCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACtt                                                                                                                                            >  1:2716327/1‑150 (MQ=255)
            acCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACttt                                                                                                                                           >  2:554201/1‑150 (MQ=255)
              cATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGc                                                                                                                                         <  2:1600980/150‑1 (MQ=255)
               aTCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGcc                                                                                                                                        >  1:3825416/1‑150 (MQ=255)
                 cGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGcccc                                                                                                                                      <  2:1857140/150‑1 (MQ=255)
                  gACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCTAGCGGCAACTTTGCCCCg                                                                                                                                     >  1:2088851/1‑150 (MQ=255)
                  gACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCTAGCGGCAACTTTGCCCCg                                                                                                                                     >  2:1033175/1‑150 (MQ=255)
                  gACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCg                                                                                                                                     >  1:1180177/1‑150 (MQ=255)
                   aCTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGc                                                                                                                                    >  1:1630984/1‑150 (MQ=255)
                      ccGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAgg                                                                                                                                 >  1:2937580/1‑150 (MQ=255)
                        gTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCTAGCGGCAACTTTGCCCCGCAGgtg                                                                                                                               <  2:1460777/150‑1 (MQ=255)
                        gTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGgtg                                                                                                                               >  2:4418989/1‑150 (MQ=255)
                         tCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGgtgt                                                                                                                              >  1:894619/1‑150 (MQ=255)
                         tCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGACAACTTTGCCCCGCAGgtgt                                                                                                                              <  2:4600236/150‑1 (MQ=255)
                          cGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTc                                                                                                                             <  1:2798669/150‑1 (MQ=255)
                          cGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTc                                                                                                                             <  1:749927/150‑1 (MQ=255)
                          cGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTc                                                                                                                             <  2:3166724/150‑1 (MQ=255)
                               aCCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCTAGCGGCAACTTTGCCCCGCAGGTGTCGGTGa                                                                                                                        <  2:2947822/150‑1 (MQ=255)
                                ccTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGaa                                                                                                                       <  2:3613140/150‑1 (MQ=255)
                                   gcgcAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAAtct                                                                                                                    <  2:2828964/150‑1 (MQ=255)
                                   gcgcAGCATGATCCAGACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAAtct                                                                                                                    <  2:3394355/150‑1 (MQ=255)
                                       agcaTGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGAGATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTg                                                                                                                >  2:3573330/1‑150 (MQ=255)
                                        gcaTGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGa                                                                                                               >  2:3264167/1‑150 (MQ=255)
                                        gcaTGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGa                                                                                                               >  1:1581422/1‑150 (MQ=255)
                                          aTGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCTAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGaaa                                                                                                             <  1:2543282/150‑1 (MQ=255)
                                            gATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGc                                                                                                           <  2:1985517/150‑1 (MQ=255)
                                             aTCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGcc                                                                                                          >  2:697080/1‑150 (MQ=255)
                                             aTCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCAATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGcc                                                                                                          <  2:1915210/150‑1 (MQ=255)
                                               ccATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCTAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTg                                                                                                        >  1:1490456/1‑150 (MQ=255)
                                               ccATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTg                                                                                                        <  2:3580327/150‑1 (MQ=255)
                                                 aTACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGgc                                                                                                      <  1:3602448/150‑1 (MQ=255)
                                                     cGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGa                                                                                                  >  2:2224286/1‑150 (MQ=255)
                                                      gTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGAc                                                                                                 >  2:2972564/1‑150 (MQ=255)
                                                      gTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGAc                                                                                                 >  2:1703831/1‑150 (MQ=255)
                                                      gTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGAc                                                                                                 <  2:4482186/150‑1 (MQ=255)
                                                         aaaGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACggg                                                                                              <  1:2480603/150‑1 (MQ=255)
                                                              tGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCTAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGGGACGt                                                                                         <  1:4369337/150‑1 (MQ=255)
                                                                 ccATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGc                                                                                      >  1:3627275/1‑150 (MQ=255)
                                                                 ccATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGc                                                                                      <  1:4146999/150‑1 (MQ=255)
                                                                  cATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCg                                                                                     >  1:4465229/1‑150 (MQ=255)
                                                                   aTCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCTAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGa                                                                                    >  1:644757/1‑150 (MQ=255)
                                                                        cACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCTAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCg                                                                               <  1:2683810/150‑1 (MQ=255)
                                                                         aCTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCgg                                                                              >  1:706511/1‑150 (MQ=255)
                                                                                  tGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTatc                                                                     >  1:1306181/1‑150 (MQ=255)
                                                                                    ctctGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTatcat                                                                   >  2:1069508/1‑150 (MQ=255)
                                                                                    ctctGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTatcat                                                                   >  2:4875740/1‑150 (MQ=255)
                                                                                     tctGCGGCTACGACGATCATCTGTTCAATCCCCTGCGGCGCGGGGGCGACGGGGCGATATCGGCTAGCGGCAACTTGGCCCCGCAGGTGGCGGTTAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTatcatc                                                                  <  2:4731236/150‑1 (MQ=255)
                                                                                         gggCTACGACTATCATGTGTTCAATCCCCTGCGGCGCGGGGGCGAGGGGGCGATATCGGCTAGCGGCAACTTGGCCCCGCCGGTGTGGGTTAATCTTCTTAACGCCGGGCGGGCGGGGGACGTGGCGAACGCGGCCGGGTATCATCAGAc                                                              <  2:4058518/149‑1 (MQ=255)
                                                                                         cGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCTAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGAc                                                              >  1:4785907/1‑150 (MQ=255)
                                                                                         cGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGAc                                                              >  1:2253501/1‑150 (MQ=255)
                                                                                           gCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCt                                                            >  2:3645599/1‑150 (MQ=255)
                                                                                            cTACGAGTATCATCTGTTCATTACCCGGGTGCTCGGCGGCGAGGGGGCGTTATCGGGGTGCGGAAACTTTGCCCCGCTGGTGTCGGTTAATGTTCTGAACGCCTGGCGCGCCGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCtt                                                           <  2:3741343/150‑1 (MQ=255)
                                                                                               cgacgaTCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTTAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTtgct                                                        <  2:757203/150‑1 (MQ=255)
                                                                                               cgacgaTCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTtgct                                                        >  1:3105665/1‑150 (MQ=255)
                                                                                                  cgaTCATCGGTTCAATACCCGGCTGCGCGGCGGCGACGGGGCGATAGCGGCGAGCGGCAACTTTGCCCCGCTGGTGTCGGTGAATCTTCTCAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCa                                                     <  2:3462016/150‑1 (MQ=255)
                                                                                                   gaTCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCTAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCaa                                                    >  2:2920779/1‑150 (MQ=255)
                                                                                                    atcatcTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCaaa                                                   >  2:4185500/1‑150 (MQ=255)
                                                                                                     tcatcTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAAt                                                  >  2:3304440/1‑150 (MQ=255)
                                                                                                     tcatcTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAAt                                                  >  2:787221/1‑150 (MQ=255)
                                                                                                     tcatcTGTTCAATACCCTGCGGCTCGGGGGCGACGGGGCTATATCGGCTCGCGGAAACTTTGCCCCGCCGGTGTCGGTTAATTTTCTGAAAGCCGGGCGCGAGGGGGAGGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAAt                                                  <  1:804089/150‑1 (MQ=255)
                                                                                                       atgtGTTCAATCCCCTGCTGCTCGGCGGCGCGGGGGCTATAGCGGCGTGCGGAAACTTGGCCCCGCTGGTGTGGTTTATTCTTCTCAAGGCGGGGGGCGAGGGGGACGTGGCGAACGCGGCCGGGTATCATCAGACCTTGCTGCAAATTc                                                <  2:2457733/147‑1 (MQ=255)
                                                                                                       atcTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTc                                                >  1:1913053/1‑150 (MQ=255)
                                                                                                            ttCAATACCGGGCTGCTCGGGGGCGACGGGGCGATATCGGCGAGGGGAAACTTTGCCCCGCTGGTGTCGGTTAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAg                                           <  2:3957946/150‑1 (MQ=255)
                                                                                                               aaTACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATg                                        >  1:4875997/1‑150 (MQ=255)
                                                                                                                   cccTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATc                                    >  1:1069983/1‑150 (MQ=255)
                                                                                                                    ccTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCa                                   >  1:2372881/1‑150 (MQ=255)
                                                                                                                      tgctgcTCGGCGGCGACGGGGCGATATCGGCTAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGc                                 >  1:3876018/1‑150 (MQ=255)
                                                                                                                        ctgcgCGGCGGCGACGGGGCGATATCGGCTAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTg                               <  2:1787260/150‑1 (MQ=255)
                                                                                                                         tgctCGGCGGCGACGGGGCGATATCGGCTAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTgg                              >  1:1367234/1‑150 (MQ=255)
                                                                                                                         tgctCGGCGGCGACGGGGCGATATCGGCTAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTgg                              >  2:143744/1‑150 (MQ=255)
                                                                                                                           ctCGGCGGCGCGGGGGCGATAGCGGCGAGCGGCAACTTGGCCCCGCAGGTGGCGGTGAATCTTCTCAAAGCCGGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGAt                            <  2:4157773/150‑1 (MQ=255)
                                                                                                                           ctCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGAt                            >  1:3688530/1‑150 (MQ=255)
                                                                                                                                 ggcgAGGGGGCTATATGGGCGAGCGGAAACTTTCCCCCGCTGTGGTGGGTTAATCTTCTCAAAGCCGGGCGCGCGGGGGCGGGGGCTAACGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCg                      <  1:3768526/150‑1 (MQ=255)
                                                                                                                                 ggcgACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCg                      <  2:4364197/150‑1 (MQ=255)
                                                                                                                                    gACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGttt                   <  2:2769042/150‑1 (MQ=255)
                                                                                                                                    gACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGttt                   >  2:2367325/1‑150 (MQ=255)
                                                                                                                                     aCGGGGCGATATCGGCTAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTtg                  >  2:112170/1‑150 (MQ=255)
                                                                                                                                       ggggCGATATCGGCGAGCGGCAACTTGGCCCCGCAGGTGTCGGTGAATCTTCTGAACGCCTGGCGCGAGGGGGACGTGGCTAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTtgtg                <  2:22808/150‑1 (MQ=255)
                                                                                                                                         ggCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGaa              <  2:2331447/150‑1 (MQ=255)
                                                                                                                                          gctatatCGGGGAGCGGCAACTTGGCCCCGCTGGTGTCGGTGATTCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAAc             <  1:1172111/147‑1 (MQ=255)
                                                                                                                                          gCGATATCGGCGCGCGGCAACTGTGCCCCGCTGGTGTCGGTGATTCTACTGAAAGCCTGGCGCGACGGGGACGTGGCGAACGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAAc             <  1:339732/150‑1 (MQ=255)
                                                                                                                                           gtttatCGGCGAGCGGAACCTTGGCCCCGCTGGTGTGGTTTAATCTTCTCAATGCGGGGCGCGCGGGGGCGGTGGCCAACGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACg            <  1:2908090/147‑1 (MQ=255)
                                                                                                                                             atatCGGCTAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTg          >  2:2073496/1‑150 (MQ=255)
                                                                                                                                               ttCGGCGAGGGTCAACTTTGCCCCGCTGGTGTGGTTGAATCTTCTGAAAGCGGGGCGCGCGGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGAt        <  1:1013639/149‑1 (MQ=255)
                                                                                                                                               atCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGAt        >  1:3700073/1‑150 (MQ=255)
                                                                                                                                               atCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGAt        <  2:1159567/150‑1 (MQ=255)
                                                                                                                                                tCGGCTCGCGGCAACTTGGCCCCGCTGGTGTCGGTGATTCTTCTGAAAGCCGGGCGCGAGGGGGAGGTGGCGAACGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGAtt       <  2:624269/150‑1 (MQ=255)
                                                                                                                                                   gcgcgcGGCAAATTTGCCCCGCTGGTGGGGGTGAATCTTCTCAAAGCCTGGCGCGCGGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGATTaaa    <  2:2895906/150‑1 (MQ=255)
                                                                                                                                                   gCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTTAAAGCCTGGCGCGACGGGGACGTGGCTAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGATTaaa    <  2:1395487/150‑1 (MQ=255)
                                                                                                                                                    cGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGATTAAag   >  2:3352537/1‑150 (MQ=255)
                                                                                                                                                     gAGGGGCAAGTTTGCCCCGCTGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGATTAAaga  <  2:2604222/150‑1 (MQ=255)
                                                                                                                                                     gAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGATTAAaga  >  1:3971853/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGCATCAAAGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCGGCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGATTAAAGA  >  CP009273/278481‑278779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑