Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,717,788 C→A 70.3% P297P (CCG→CCT)  glyQ ← glycine tRNA synthetase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,717,7880CA70.3% 530.7 / inf 383P297P (CCG→CCT) glyQglycine tRNA synthetase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (59/55);  new base A (144/125);  total (203/180)
Fisher's exact test for biased strand distribution p-value = 8.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 386 total aligned reads displayed.

AGGCCAGCGTTATCCAGCTCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTGGGCGGCTTTCAG  >  CP009273/3717639‑3717934
                                                                                                                                                     |                                                                                                                                                  
     aGCGTTATCCAGCTCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGaa                                                                                                                                               >  1:3194642/1‑150 (MQ=255)
     aGCGTTATCCAGCTCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGaa                                                                                                                                               >  1:4212797/1‑150 (MQ=255)
     aGCGTTATCCAGCTCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGaa                                                                                                                                               <  2:3942031/150‑1 (MQ=255)
     aGCGTTATCCAGCTCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGaa                                                                                                                                               <  2:1701083/150‑1 (MQ=255)
     aGCGTTATCCAGCTCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGaa                                                                                                                                               >  1:1365242/1‑150 (MQ=255)
              cAGCTCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGc                                                                                                                                      >  2:639167/1‑150 (MQ=255)
               aGCTCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCt                                                                                                                                     >  1:4435706/1‑150 (MQ=255)
                  tCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGGAGCCGAGGGCTTCa                                                                                                                                  >  1:4130117/1‑150 (MQ=255)
                    cGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACg                                                                                                                                <  1:3106634/150‑1 (MQ=255)
                       aGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGa                                                                                                                             <  1:32541/150‑1 (MQ=255)
                       aGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGa                                                                                                                             >  2:914882/1‑150 (MQ=255)
                          aaaGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGc                                                                                                                          >  2:2725830/1‑150 (MQ=255)
                              ttCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCgtag                                                                                                                      <  1:4482328/150‑1 (MQ=255)
                              ttCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCgtag                                                                                                                      <  1:3678537/150‑1 (MQ=255)
                               tCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCgtagt                                                                                                                     <  1:2474848/150‑1 (MQ=255)
                                cGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCgtagta                                                                                                                    <  1:277230/150‑1 (MQ=255)
                                   agcaAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGc                                                                                                                 >  1:1657629/1‑150 (MQ=255)
                                     caAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCtt                                                                                                               <  1:1436510/150‑1 (MQ=255)
                                      aaaGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTc                                                                                                              >  2:3857789/1‑150 (MQ=255)
                                       aaGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCt                                                                                                             <  1:1144964/150‑1 (MQ=255)
                                        aGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTg                                                                                                            >  1:1249470/1‑150 (MQ=255)
                                          gACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGcc                                                                                                          >  1:3230514/1‑150 (MQ=255)
                                           aCTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCa                                                                                                         <  2:4370963/150‑1 (MQ=255)
                                            cTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCAc                                                                                                        >  1:1940403/1‑150 (MQ=255)
                                              cAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTg                                                                                                      >  2:2316892/1‑150 (MQ=255)
                                                   aGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTg                                                                                                 >  1:2359437/1‑150 (MQ=255)
                                                    ggCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTgg                                                                                                <  1:2909604/150‑1 (MQ=255)
                                                     gCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGt                                                                                               <  1:3459977/150‑1 (MQ=255)
                                                          gcAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAggg                                                                                          <  2:447338/150‑1 (MQ=255)
                                                           cAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGt                                                                                         <  2:2635700/150‑1 (MQ=255)
                                                               gCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGa                                                                                     <  2:3474304/150‑1 (MQ=255)
                                                               gCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGa                                                                                     <  1:387241/150‑1 (MQ=255)
                                                               gCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGa                                                                                     >  1:3611341/1‑150 (MQ=255)
                                                                  tttGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATg                                                                                  <  1:3995866/150‑1 (MQ=255)
                                                                      gtggCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCa                                                                              <  1:2065609/150‑1 (MQ=255)
                                                                       tggCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAg                                                                             >  2:66563/1‑150 (MQ=255)
                                                                          cAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAAt                                                                          <  2:1643599/150‑1 (MQ=255)
                                                                          cAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAAt                                                                          <  1:1708207/150‑1 (MQ=255)
                                                                           aGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATg                                                                         >  1:2976688/1‑150 (MQ=255)
                                                                           aGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATg                                                                         >  1:4752700/1‑150 (MQ=255)
                                                                           aGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATg                                                                         >  1:4555339/1‑150 (MQ=255)
                                                                            gCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGt                                                                        <  2:343053/150‑1 (MQ=255)
                                                                            gCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGt                                                                        <  2:461796/150‑1 (MQ=255)
                                                                              tctTCAGTGCCGATTTCCACCAGACAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGACTGCGCAGAATGTAg                                                                      >  2:3662026/1‑150 (MQ=255)
                                                                              tctTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAg                                                                      <  2:1995754/150‑1 (MQ=255)
                                                                              tctTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAg                                                                      <  2:2970176/150‑1 (MQ=255)
                                                                              tctTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAg                                                                      <  1:3919660/150‑1 (MQ=255)
                                                                                 tCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAgcgc                                                                   >  2:2602012/1‑150 (MQ=255)
                                                                                   aGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTg                                                                 >  1:2180095/1‑150 (MQ=255)
                                                                                    gTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGa                                                                >  2:4417282/1‑150 (MQ=255)
                                                                                     tGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGAc                                                               >  1:595219/1‑150 (MQ=255)
                                                                                     tGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGAc                                                               <  2:344147/150‑1 (MQ=255)
                                                                                      gCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACg                                                              <  2:2082567/150‑1 (MQ=255)
                                                                                        cGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCt                                                            >  2:3535684/1‑150 (MQ=255)
                                                                                        cGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCt                                                            >  1:843549/1‑150 (MQ=255)
                                                                                          aTTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCg                                                          <  1:2602139/150‑1 (MQ=255)
                                                                                           tttCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCgg                                                         >  2:1891946/1‑150 (MQ=255)
                                                                                            ttCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGt                                                        <  2:1366033/150‑1 (MQ=255)
                                                                                            ttCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGt                                                        >  1:2861158/1‑150 (MQ=255)
                                                                                            ttCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGt                                                        >  1:2569802/1‑150 (MQ=255)
                                                                                             tCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTg                                                       <  1:2196632/150‑1 (MQ=255)
                                                                                              ccaccaGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGa                                                      <  1:1306400/150‑1 (MQ=255)
                                                                                              ccaccaGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGa                                                      >  1:3636046/1‑150 (MQ=255)
                                                                                                accaGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACg                                                    <  2:4750745/150‑1 (MQ=255)
                                                                                                accaGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACg                                                    <  2:3248806/150‑1 (MQ=255)
                                                                                                accaGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACg                                                    >  2:2139068/1‑150 (MQ=255)
                                                                                                accaGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACg                                                    >  2:1555427/1‑150 (MQ=255)
                                                                                                 ccaGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACgg                                                   >  1:960838/1‑150 (MQ=255)
                                                                                                      aaaGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATg                                              >  1:1200638/1‑150 (MQ=255)
                                                                                                      aaaGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATg                                              >  1:2742529/1‑150 (MQ=255)
                                                                                                            ttCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTa                                        <  1:3478773/150‑1 (MQ=255)
                                                                                                              ctcAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTAcg                                      <  2:1736372/150‑1 (MQ=255)
                                                                                                                cAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTAcgcg                                    >  2:531041/1‑150 (MQ=255)
                                                                                                                cAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTAcgcg                                    >  1:1567995/1‑150 (MQ=255)
                                                                                                                  gACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCa                                  >  2:2871315/1‑150 (MQ=255)
                                                                                                                   aCATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGACGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCAt                                 >  2:4343322/1‑150 (MQ=255)
                                                                                                                   aCATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCAt                                 <  2:3361435/150‑1 (MQ=255)
                                                                                                                    cATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATc                                >  2:248565/1‑150 (MQ=255)
                                                                                                                      tAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCca                              >  1:1088585/1‑150 (MQ=255)
                                                                                                                       aGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCcag                             <  2:2247024/150‑1 (MQ=255)
                                                                                                                       aGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCcag                             >  1:3536112/1‑150 (MQ=255)
                                                                                                                         ccgccTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCcagca                           <  2:2541932/150‑1 (MQ=255)
                                                                                                                             ctctTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGtt                       <  2:3194642/150‑1 (MQ=255)
                                                                                                                                ttacttaTCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGaa                    >  1:481876/1‑150 (MQ=255)
                                                                                                                                  acttaTCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGc                  >  2:1326086/1‑150 (MQ=255)
                                                                                                                                  acttaTCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGc                  >  2:4724363/1‑150 (MQ=255)
                                                                                                                                   cttaTCTGTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCt                 <  2:2994711/150‑1 (MQ=255)
                                                                                                                                    ttaTCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCtg                <  2:3136058/150‑1 (MQ=255)
                                                                                                                                      aTCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCtgtg              <  2:3993318/150‑1 (MQ=255)
                                                                                                                                       tCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTgg             >  1:1506903/1‑150 (MQ=255)
                                                                                                                                        cTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTggg            <  1:4663145/150‑1 (MQ=255)
                                                                                                                                        cTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTggg            <  2:118755/150‑1 (MQ=255)
                                                                                                                                         tttGTTGCACCTCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGGATGCTTCGGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGGAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTGggc           <  2:4212797/150‑1 (MQ=255)
                                                                                                                                         tttGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTGggc           >  2:984048/1‑150 (MQ=255)
                                                                                                                                         gttgttgCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAAGGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTGggc           <  2:4099482/149‑1 (MQ=255)
                                                                                                                                           tgttgCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTGggcgg         <  1:333129/150‑1 (MQ=255)
                                                                                                                                                cacaTCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTGGGCGGCTTTc    <  1:4724359/150‑1 (MQ=255)
                                                                                                                                                cacaTCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTGGGCGGCTTTc    >  2:1746300/1‑150 (MQ=255)
                                                                                                                                                 acaTAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTGGGCGGCTTTCa   <  1:1849848/150‑1 (MQ=255)
                                                                                                                                                  caTAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTGGGCGGCTTTCAg  <  1:424826/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
AGGCCAGCGTTATCCAGCTCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTGGGCGGCTTTCAG  >  CP009273/3717639‑3717934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑