BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,860,159	C→A	64.8%	P33P (CCG→CCT)	ibpB ←	heat shock chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,860,159	0	C	A	64.8%	198.7 / 287.5	266	P33P (CCG→CCT)	ibpB	heat shock chaperone
Reads supporting (aligned to +/- strand): ref base C (44/48); new base A (65/105); total (113/153)
Fisher's exact test for biased strand distribution p-value = 1.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.17e-01

Only 100 of 274 total aligned reads displayed.

TTTTTTCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTC  >  CP009273/3860012‑3860308

ttttttCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTAcggagg                                                                                                                                                      <  1:992982/150‑1 (MQ=255)

   tttCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGaa                                                                                                                                                   <  1:1093303/150‑1 (MQ=255)

    ttCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCGCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCGTTTCTCCATGTTGTACGGCGGGAAg                                                                                                                                                  >  2:3567387/1‑150 (MQ=255)

       tcttctTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAATGTTGTAAGGAGGGAAGctc                                                                                                                                               >  2:1044715/1‑150 (MQ=255)

        cttcttTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGctct                                                                                                                                              <  2:1958697/150‑1 (MQ=255)

        cttcttTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGctct                                                                                                                                              <  2:652856/150‑1 (MQ=255)

        cttcttTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGctct                                                                                                                                              <  2:979872/150‑1 (MQ=255)

        cttcttTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGctct                                                                                                                                              <  2:1865232/150‑1 (MQ=255)

        cttcttTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGctct                                                                                                                                              <  2:57423/150‑1 (MQ=255)

         ttcttTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTGTCAATGTTGTTCGGAGGGGAGcgggg                                                                                                                                             >  1:4080810/1‑146 (MQ=255)

         ttcttTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTg                                                                                                                                             <  2:3074962/150‑1 (MQ=255)

          tcttTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTgg                                                                                                                                            <  2:4720593/150‑1 (MQ=255)

           cttTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTTTCAATGGTGTTGGGCGGGTAGCGATGGc                                                                                                                                           >  1:3753907/1‑150 (MQ=255)

           cttTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTGTCAAAGTTGTTAGGCGGGAAGCTCTGGc                                                                                                                                           >  1:2267415/1‑150 (MQ=255)

           cttTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATGAATGTTTTTCGGCGGGACGGGCTGGc                                                                                                                                           >  1:2145389/1‑150 (MQ=255)

           cttTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATAAATGTTGTACGGAGGGAAGCTCTGGc                                                                                                                                           >  1:2766039/1‑150 (MQ=255)

             tttGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCtt                                                                                                                                         <  1:4100262/150‑1 (MQ=255)

               tGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTc                                                                                                                                       >  2:4441597/1‑150 (MQ=255)

               tGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATAAAATTTTTAAGGTGGGAAGCGATGGCttta                                                                                                                                       >  1:4514826/1‑149 (MQ=255)

               tGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCAGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTc                                                                                                                                       <  2:1561943/150‑1 (MQ=255)

                 gctgctCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCAc                                                                                                                                     <  2:4419291/150‑1 (MQ=255)

                   tgctCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCAGGCTTTCaacc                                                                                                                                   >  2:441098/1‑147 (MQ=255)

                       ccGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGt                                                                                                                               <  1:2458679/150‑1 (MQ=255)

                        cGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATAAATGTTGTAAGGCGGGAAGCTCTGGCTTTAACCGGCGtt                                                                                                                              >  2:3536226/1‑150 (MQ=255)

                         ggCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGttt                                                                                                                             <  2:4155124/150‑1 (MQ=255)

                           cGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTg                                                                                                                           <  1:3327777/150‑1 (MQ=255)

                           cGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTg                                                                                                                           <  1:1451593/150‑1 (MQ=255)

                           cGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTg                                                                                                                           <  2:1948830/150‑1 (MQ=255)

                           cGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTg                                                                                                                           <  2:112471/150‑1 (MQ=255)

                            gTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGc                                                                                                                          >  1:3608499/1‑150 (MQ=255)

                            gTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCGTTTATCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGc                                                                                                                          >  2:767335/1‑150 (MQ=255)

                             tGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCa                                                                                                                         <  1:3439248/150‑1 (MQ=255)

                               ccTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGGTTTTCAGt                                                                                                                       >  1:1794488/1‑150 (MQ=255)

                                cTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAATGTTGTACGGAGGGAAGCACTGGCTTTGACCGGCGgttttgagtt                                                                                                                      >  1:2230364/1‑144 (MQ=255)

                                   ttACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTGAATGTTTTACGGAGGGAAGCTCTGGCGTTCACCCGCGTTTTGCAGTGC‑Gt                                                                                                                  >  1:2774505/1‑150 (MQ=255)

                                      cGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAACTCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTgg                                                                                                               <  2:4830086/150‑1 (MQ=255)

                                      cGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTAAGGAGGGAAGCTCTGGCTTTAACCGGCGTTTTGCAGTGC‑GTTgg                                                                                                               >  2:3160009/1‑150 (MQ=255)

                                      cGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATAAATGTTGTACGGCGGGTAGCTCTGGGGTTCACCGGCGGTTTTGAGTGC‑GGTgg                                                                                                               >  2:2485993/1‑150 (MQ=255)

                                        cTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCCGCGTTTTGAACTGC‑GTTGGcc                                                                                                             >  1:335313/1‑150 (MQ=255)

                                         tCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCa                                                                                                            <  2:3453394/150‑1 (MQ=255)

                                                cgTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTc                                                                                                     <  2:2396237/150‑1 (MQ=255)

                                                cgTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTc                                                                                                     <  2:1338946/150‑1 (MQ=255)

                                                      ttCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTGTAAATGTTGTACGGAGGGAAGCTATGGCTGTCACCGGCGTGTTTGAGTTCGGTTGCCCCGTTT‑TCAAAAca                                                                                               >  1:4705237/1‑149 (MQ=255)

                                                      ttCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAAcc                                                                                               <  2:2154354/150‑1 (MQ=255)

                                                           gTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATcc                                                                                          <  1:2894174/150‑1 (MQ=255)

                                                            ttGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCa                                                                                         <  2:1360967/150‑1 (MQ=255)

                                                              gAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGGGGTTATCGTCGCTTGTGTACATGTTTTTCGGCGGGGACCTCTGGCTGTAAACGGAGTTGTTAAGTGC‑GGATGGCACTTATTCAAAAACCATCCAtt                                                                                       >  2:643131/1‑150 (MQ=255)

                                                                aTCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGa                                                                                     <  1:2469221/150‑1 (MQ=255)

                                                                   tctAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGc                                                                                  <  1:2365824/150‑1 (MQ=255)

                                                                      taaTCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCGGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCCGTTTGTAAAAACCGAACCATTGACGCATc                                                                               >  2:4038537/2‑150 (MQ=255)

                                                                       aaTCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTGTCAATGTTGTACGGCGGGAAGCTCTGGCGTTCAACGGCGTTTTGCAGTGC‑GGTGGCCAGTTTCTCAAAAACCATCCAGTGACGCATCa                                                                              >  2:2247722/1‑150 (MQ=255)

                                                                        aTCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAg                                                                             <  1:1778294/150‑1 (MQ=255)

                                                                         tCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGt                                                                            <  2:431081/150‑1 (MQ=255)

                                                                          cTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTg                                                                           <  2:1900198/150‑1 (MQ=255)

                                                                            tCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTggg                                                                         <  1:331715/150‑1 (MQ=255)

                                                                              cTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGa                                                                       <  1:3113121/150‑1 (MQ=255)

                                                                              cTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAAATTTTTAAGGAGGGAAGCACTGGGTTTAACCGGCGTTTTGGCTTGG‑GTTTGCCAATTTGTAAAAAACAAAAAATTGAAGCAACACTGGGGa                                                                       >  2:1020143/1‑150 (MQ=255)

                                                                               tGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGAt                                                                      <  1:3470230/150‑1 (MQ=255)

                                                                               tGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCGTTTCTCACAGTTGTACGGCGGGACGCTCTGGCGTTCACCCGCGGTTTGCAGTGC‑GGTGGCCCCGTTGTCCAAACCCATCCAATGACCCAGCAggggggtg                                                                      >  2:2878011/1‑148 (MQ=255)

                                                                                   ggAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAAt                                                                  <  2:3549065/150‑1 (MQ=255)

                                                                                   ggAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAAt                                                                  <  1:1891703/150‑1 (MQ=255)

                                                                                     aaaCCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCg                                                                <  2:941229/150‑1 (MQ=255)

                                                                                           gCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTa                                                          <  1:2633624/150‑1 (MQ=255)

                                                                                            ccAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCGTTTATAACTGTTGTTCGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTTCAGTGC‑GTTGGCCAGTTTGTCAAAAAACATCCAATTACGCATAAGTGGGGCTAAATCGAATTTAc                                                         >  2:1666145/1‑150 (MQ=255)

                                                                                            ccAGCGCAAGGGTAATGCGGTAGTGGTTAACGTCGCGTTTCACACTTTTGTAAGGAGGGAAGCTCTGGCTTTAACCCGCGTTTTTCATAGC‑CTTGGGCACTTTTTCAAAAACGATAAATTGACGCATAACTGGGGAAAAATCCAACTTAc                                                         >  2:2658028/1‑150 (MQ=255)

                                                                                             cAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACg                                                        <  1:507347/150‑1 (MQ=255)

                                                                                             cAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAAAGTTGTACGGAGGGAAGCTCTGGCGTTAAACCGCGGTTTTCAGTGC‑GTTTGGCCGTTTTTCAAAAACCCTACAATGACCCAGAAGTGGGGTTAAAAAGAAGtttac                                                        >  2:3347535/1‑147 (MQ=255)

                                                                                               gcgcAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCa                                                      <  2:3214251/150‑1 (MQ=255)

                                                                                               gcgcAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCa                                                      <  2:3917655/150‑1 (MQ=255)

                                                                                                cgcAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCAt                                                     <  2:290094/150‑1 (MQ=255)

                                                                                                cgcAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCAt                                                     <  2:1192217/150‑1 (MQ=255)

                                                                                                 gcAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATa                                                    <  1:2279756/150‑1 (MQ=255)

                                                                                                   aaGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGt                                                  <  1:4504953/150‑1 (MQ=255)

                                                                                                     gggTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCa                                                <  2:4273051/150‑1 (MQ=255)

                                                                                                     gggTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCa                                                <  2:912622/150‑1 (MQ=255)

                                                                                                       gTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCAtt                                              <  1:1301087/150‑1 (MQ=255)

                                                                                                        tAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCAttt                                             <  1:4441597/150‑1 (MQ=255)

                                                                                                          aTGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGGACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTtct                                           <  2:87817/150‑1 (MQ=255)

                                                                                                            gCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTcc                                         <  2:4735988/150‑1 (MQ=255)

                                                                                                               gTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTc                                      <  2:3608499/150‑1 (MQ=255)

                                                                                                                tAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCt                                     <  1:4795593/150‑1 (MQ=255)

                                                                                                                     gTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTaagaa                                <  2:1996386/150‑1 (MQ=255)

                                                                                                                      ttATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTaagaag                               <  1:3241279/150‑1 (MQ=255)

                                                                                                                      ttATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTaagaag                               <  2:2267724/150‑1 (MQ=255)

                                                                                                                           gtcgCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGagt                          <  2:3259005/150‑1 (MQ=255)

                                                                                                                             cgCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTTC‑GTTTGCCAGTTTGTCAAAAACGATCCATTGAAGCATCAGTTGGGATAAATAGAAGTTAAGCATAGTAATTTCTACTTCTAAGAATCGagtaa                        >  1:498284/1‑150 (MQ=255)

                                                                                                                              gCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGagtaag                       >  1:1925193/1‑150 (MQ=255)

                                                                                                                              gCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGagtaag                       <  2:4688935/150‑1 (MQ=255)

                                                                                                                                 tttCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTAc                    >  2:37881/1‑150 (MQ=255)

                                                                                                                                   tctcAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCt                  <  2:1759673/150‑1 (MQ=255)

                                                                                                                                   tctcAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCt                  >  2:2755670/1‑150 (MQ=255)

                                                                                                                                    ctcAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTg                 >  2:4519885/1‑150 (MQ=255)

                                                                                                                                    ctcAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTg                 <  1:4333149/150‑1 (MQ=255)

                                                                                                                                     tcAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGc                >  1:1533436/1‑150 (MQ=255)

                                                                                                                                     tcAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTTCAGTGC‑GTTTGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGc                >  1:2062408/1‑150 (MQ=255)

                                                                                                                                      cAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCa               >  2:3025539/1‑150 (MQ=255)

                                                                                                                                      cAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCa               <  1:746644/150‑1 (MQ=255)

                                                                                                                                      cAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCa               >  1:1148450/1‑150 (MQ=255)

                                                                                                                                              tACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGaa       >  2:1414035/1‑150 (MQ=255)

                                                                                                                                                cggaggGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GGTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGTAGTTACGCATAGTCATTACTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCcgacga     >  2:1845101/1‑150 (MQ=255)

TTTTTTCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC‑GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTC  >  CP009273/3860012‑3860308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`