Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,871,570 A→C 25.1% L637R (CTG→CGG)  gyrB ← DNA gyrase, subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,871,5700AC25.1% 600.6 / 296.2 338L637R (CTG→CGG) gyrBDNA gyrase, subunit B
Reads supporting (aligned to +/- strand):  ref base A (112/141);  new base C (43/42);  total (155/183)
Fisher's exact test for biased strand distribution p-value = 3.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01

Only 100 of 343 total aligned reads displayed.

ATTCGCCACCGGTGATAAACTCGTGATCCAGCGGATAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTG  >  CP009273/3871421‑3871719
                                                                                                                                                     |                                                                                                                                                     
 ttCGCCACCGGTGATAAACTCGTGATCCAGCGGATAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAg                                                                                                                                                      >  2:3170621/1‑150 (MQ=255)
 ttCGCCACCGGTGATAAACTCGTGATCCAGCGGATAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAg                                                                                                                                                      >  2:1592377/1‑150 (MQ=255)
  tCGCCACCGGTGATAAACTCGTGATCCAGCGGATAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCCGt                                                                                                                                                     >  2:490348/1‑150 (MQ=255)
     ccACCGGTGATAAACTCGTGATCCAGCGGATAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCg                                                                                                                                                  <  1:4017040/150‑1 (MQ=255)
     ccACCGGTGATAAACTCGTGATCCAGCGGATAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTGCTTTGTCGTTCAGTTCg                                                                                                                                                  >  2:2784094/1‑150 (MQ=255)
        ccGGTGATAAACTCGTGATCCAGCGGATAGTCAGTATCCACACCGTGGGTACGTACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCCGTTCGCTg                                                                                                                                               >  2:952368/1‑150 (MQ=255)
        ccGGTGATAAACTCGTGATCCAGCGGATAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCCGTTCGCTg                                                                                                                                               >  1:936129/1‑150 (MQ=255)
        ccGGTGATAAACTCGTGATCCAGCGGATAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCCGTTCGCTg                                                                                                                                               >  1:4572862/1‑150 (MQ=255)
          ggTGATAAACTCGTGATCCAGCGGATAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTACGTTCGCTGAc                                                                                                                                             >  2:3141296/1‑150 (MQ=255)
            tGATAAACTCGTGATCCAGCGGATAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCa                                                                                                                                           <  1:1873066/150‑1 (MQ=255)
             gATAAACTCGTGATCCAGCGGATAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAg                                                                                                                                          >  1:1528687/1‑150 (MQ=255)
             gATAAACTCGTGATCCAGCGGATAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAg                                                                                                                                          <  1:3307100/150‑1 (MQ=255)
             gATAAACTCGTGATCCAGCGGATAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAg                                                                                                                                          <  1:2227770/150‑1 (MQ=255)
              aTAAACTCGTGATCCAGCGGATAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAgc                                                                                                                                         <  2:2063918/150‑1 (MQ=255)
                     cGTGATCCAGCGGATAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCa                                                                                                                                  <  1:1403022/150‑1 (MQ=255)
                     cGTGATCCAGCGGATAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCa                                                                                                                                  >  1:4590084/1‑150 (MQ=255)
                         aTCCAGCGGATAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCa                                                                                                                              >  1:3433118/1‑150 (MQ=255)
                         aTCCAGCGGATAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCa                                                                                                                              <  1:3597244/150‑1 (MQ=255)
                            cAGCGGATAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCCGTTCGCTGACCAGCGCGTTCACCCAGCg                                                                                                                           <  2:4028682/150‑1 (MQ=255)
                            cAGCGGATAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCg                                                                                                                           <  2:1653558/150‑1 (MQ=255)
                            cAGCGGATAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCg                                                                                                                           >  2:1472133/1‑150 (MQ=255)
                              gCGGATAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCCGTTCGCTGACCAGCGCGTTCACCCAGCggg                                                                                                                         >  2:3726288/1‑150 (MQ=255)
                               cGGATAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGt                                                                                                                        <  1:3831404/150‑1 (MQ=255)
                               cGGATAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGt                                                                                                                        <  1:3683662/150‑1 (MQ=255)
                               cGGATAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGt                                                                                                                        >  1:4767189/1‑150 (MQ=255)
                                ggATAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTa                                                                                                                       <  2:3042501/150‑1 (MQ=255)
                                 gATAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTaa                                                                                                                      <  2:3299052/150‑1 (MQ=255)
                                  aTAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAAc                                                                                                                     >  1:4416840/1‑150 (MQ=255)
                                  aTAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAAc                                                                                                                     <  2:3413199/150‑1 (MQ=255)
                                    aGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCCGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACgg                                                                                                                   >  1:3807191/1‑150 (MQ=255)
                                    aGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCCGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACgg                                                                                                                   <  2:4479782/150‑1 (MQ=255)
                                       cAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCCGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCt                                                                                                                >  2:4560698/1‑150 (MQ=255)
                                       cAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCt                                                                                                                <  1:2075471/150‑1 (MQ=255)
                                        aGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTTCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAAAGGTCTg                                                                                                               >  1:4848230/1‑150 (MQ=255)
                                          tATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCt                                                                                                             >  1:1993327/1‑150 (MQ=255)
                                              cacaCCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCtcatc                                                                                                         >  2:1683336/1‑150 (MQ=255)
                                                  ccGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGAGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGaa                                                                                                     >  2:2406873/1‑150 (MQ=255)
                                                    gTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCCGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAg                                                                                                   <  1:3907869/150‑1 (MQ=255)
                                                        gTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCa                                                                                               <  1:1825272/150‑1 (MQ=255)
                                                        gTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCa                                                                                               <  1:1898140/150‑1 (MQ=255)
                                                          acgcacgcGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGc                                                                                             <  2:1052298/150‑1 (MQ=255)
                                                            gcacgcGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCtt                                                                                           <  1:863350/150‑1 (MQ=255)
                                                            gcacgcGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCtt                                                                                           >  1:2452018/1‑150 (MQ=255)
                                                             cacgcGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCCGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTc                                                                                          <  2:2030551/150‑1 (MQ=255)
                                                             cacgcGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTc                                                                                          >  1:2670030/1‑150 (MQ=255)
                                                                 cgAACAATCGGCTCGAACAGGTTTTGCCCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTc                                                                                      <  1:1512073/150‑1 (MQ=255)
                                                                  gAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCCGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCa                                                                                     >  1:4059283/1‑150 (MQ=255)
                                                                     cAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACg                                                                                  >  1:300367/1‑150 (MQ=255)
                                                                       aTCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTc                                                                                <  1:1310079/150‑1 (MQ=255)
                                                                       aTCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTc                                                                                <  1:2649750/150‑1 (MQ=255)
                                                                          ggCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGc                                                                             >  2:2505299/1‑150 (MQ=255)
                                                                           gCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCt                                                                            <  2:3368264/150‑1 (MQ=255)
                                                                             tCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTga                                                                          <  1:4084980/150‑1 (MQ=255)
                                                                              cGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCCGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTgat                                                                         >  1:3577171/1‑150 (MQ=255)
                                                                              cGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTgat                                                                         <  2:4103549/150‑1 (MQ=255)
                                                                               gAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCCGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTgata                                                                        <  2:3826353/150‑1 (MQ=255)
                                                                                  cAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCCGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTgatagat                                                                     <  2:1258502/150‑1 (MQ=255)
                                                                                  cAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCCGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTgatagat                                                                     <  2:3934793/150‑1 (MQ=255)
                                                                                   aGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCCGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTgatagata                                                                    >  2:4744636/1‑150 (MQ=255)
                                                                                   aGGTTTTGCTCAGCATTGGTGTGAACATCAAACGTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTgatagata                                                                    <  2:2705499/150‑1 (MQ=255)
                                                                                       tttGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCCGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGct                                                                >  2:4448677/1‑150 (MQ=255)
                                                                                       tttGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGct                                                                <  1:2839479/150‑1 (MQ=255)
                                                                                              aGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAg                                                         <  1:3067502/150‑1 (MQ=255)
                                                                                                 aTTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCAt                                                      >  2:733851/1‑150 (MQ=255)
                                                                                                    ggTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCCGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGc                                                   <  1:1195572/150‑1 (MQ=255)
                                                                                                     gtgtGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCCGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCt                                                  <  2:2249417/150‑1 (MQ=255)
                                                                                                      tgtgAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCtt                                                 >  2:4158873/1‑150 (MQ=255)
                                                                                                      tgtgAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCtt                                                 >  2:1323318/1‑150 (MQ=255)
                                                                                                          aaCATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCgg                                             <  1:3157005/150‑1 (MQ=255)
                                                                                                          aaCATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCgg                                             <  1:4673731/150‑1 (MQ=255)
                                                                                                            cATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGAt                                           <  2:1528687/150‑1 (MQ=255)
                                                                                                            cATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTACTTTCGGAt                                           >  2:4785776/1‑150 (MQ=255)
                                                                                                              tCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATaa                                         <  1:4152022/150‑1 (MQ=255)
                                                                                                                aaaCTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCCGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAacg                                       >  2:891181/1‑150 (MQ=255)
                                                                                                                aaaCTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAacg                                       >  2:1331157/1‑150 (MQ=255)
                                                                                                                  aCTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAacgac                                     <  2:1394462/150‑1 (MQ=255)
                                                                                                                     tCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCt                                  <  2:4361254/150‑1 (MQ=255)
                                                                                                                      ccACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTc                                 <  1:1341497/150‑1 (MQ=255)
                                                                                                                      ccACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTc                                 <  1:2514933/150‑1 (MQ=255)
                                                                                                                          tGGCTGCCGTGCTGTTCTTTGTCGTTCCGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATa                             <  1:1663090/150‑1 (MQ=255)
                                                                                                                          tGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATa                             <  2:255885/150‑1 (MQ=255)
                                                                                                                          tGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATa                             >  2:2161834/1‑150 (MQ=255)
                                                                                                                              tGCCGTGCTGTTCTTTGTCGTTCCGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGAt                         >  2:3433213/1‑150 (MQ=255)
                                                                                                                              tGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGAt                         >  2:4381703/1‑150 (MQ=255)
                                                                                                                                 cGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCCGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGa                      <  2:2959079/150‑1 (MQ=255)
                                                                                                                                 cGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGa                      >  1:2782666/1‑150 (MQ=255)
                                                                                                                                   tGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATc                    <  1:622710/150‑1 (MQ=255)
                                                                                                                                   tGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATc                    >  2:539529/1‑150 (MQ=255)
                                                                                                                                      tGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAATGGTCAGATTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCAtt                 <  1:10062/150‑1 (MQ=255)
                                                                                                                                      tGTTATTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATAAGATTGATCAtt                 >  2:3552352/1‑150 (MQ=255)
                                                                                                                                       gTTCTTTGTCGTTCCGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCAttt                <  1:3726288/150‑1 (MQ=255)
                                                                                                                                        ttCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCAtttt               >  2:3647449/1‑150 (MQ=255)
                                                                                                                                        ttCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCAtttt               <  2:3592340/150‑1 (MQ=255)
                                                                                                                                         tCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTc              <  2:307165/150‑1 (MQ=255)
                                                                                                                                          cTTTGTCGTTCCGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCt             <  1:2177576/150‑1 (MQ=255)
                                                                                                                                          cTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCt             <  1:4476423/150‑1 (MQ=255)
                                                                                                                                            ttGTCGTTCCGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGc           >  1:2981164/1‑150 (MQ=255)
                                                                                                                                               tCGTTCCGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTc        <  2:649872/150‑1 (MQ=255)
                                                                                                                                                cGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTcg       >  2:4214852/1‑150 (MQ=255)
                                                                                                                                                   tCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGt    <  2:1857466/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATTCGCCACCGGTGATAAACTCGTGATCCAGCGGATAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTG  >  CP009273/3871421‑3871719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑