Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 283,636 A→C 13.3% Q379H (CAA→CAC)  yagH → CP4‑6 prophage; putative xylosidase/arabinosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273283,6360AC13.3% 724.7 / 28.3 319Q379H (CAA→CAC) yagHCP4‑6 prophage; putative xylosidase/arabinosidase
Reads supporting (aligned to +/- strand):  ref base A (166/102);  new base C (2/49);  total (168/151)
Fisher's exact test for biased strand distribution p-value = 5.45e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 328 total aligned reads displayed.

AACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGG  >  CP009273/283487‑283785
                                                                                                                                                      |                                                                                                                                                     
aaCTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCaa                                                                                                                                                       >  1:3523118/1‑150 (MQ=255)
  cTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATc                                                                                                                                                     <  2:3969744/150‑1 (MQ=255)
    ggCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGa                                                                                                                                                   >  2:3148053/1‑150 (MQ=255)
          aCGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTgg                                                                                                                                             >  2:230063/1‑150 (MQ=255)
           ctttttCGACGCCTTTTCGTTTGACCGGGTGGTGCTGACCCTGCGATTTCGTTTCGACTACACCCTCGGGTCGCTCACCGCGCGCCCGGGATTATTAGGGCTATTTGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGgc                                                                                                                                            <  2:3792420/147‑1 (MQ=255)
            gATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGgcg                                                                                                                                           >  1:666675/1‑150 (MQ=255)
                 cGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGcgccg                                                                                                                                      >  2:4530361/1‑150 (MQ=255)
                       ctgTTCGCTTGACCGGGAGCGGCAGACCCGGCGCTTTCCGTTCGACGACACCCGCGGCTCGCTCACCGCGCGCCGGGGATTCTTCCGGCTCTTTGGC‑AACGACTCGCTCAATTCGACCTTCCCCCACTCGACCGTGGCGCGCCGCTGgca                                                                                                                                <  2:3350461/148‑1 (MQ=255)
                        aGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCCTTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTACACCCAAACGACCGTGCCGCGCCGCTGgcag                                                                                                                               >  1:1446497/1‑150 (MQ=255)
                        aGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGgcag                                                                                                                               >  1:2024383/1‑150 (MQ=255)
                        aGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGgcag                                                                                                                               >  1:3954016/1‑150 (MQ=255)
                         gTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGgcagc                                                                                                                              >  2:2839150/1‑150 (MQ=255)
                         gTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGgcagc                                                                                                                              >  1:2599635/1‑150 (MQ=255)
                          ttCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCACTTCGACCTTCACCCACTCGACCGTGGCGCGCCGCTGgcagca                                                                                                                             >  2:4547319/1‑150 (MQ=255)
                             gCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACtt                                                                                                                          >  2:796741/1‑150 (MQ=255)
                              cTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTc                                                                                                                         >  2:529998/1‑150 (MQ=255)
                                   cccGGAGCTGCAGACCCTGCGCATTCCTTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCtt                                                                                                                    <  2:1493721/150‑1 (MQ=255)
                                     cGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTcc                                                                                                                  >  1:2192154/1‑150 (MQ=255)
                                        tgcggCAGACCCTGCGCTTTCGGTTCGCCTACACCCGCGGCGCGCTCACCGCGCGCCCGGGCTTATTACGGCTCTTTGGC‑AACGACTCGCTCAATTCGACCTTCACCCACTCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCggg                                                                                                               <  2:1309400/146‑1 (MQ=255)
                                        aGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCggg                                                                                                               >  2:4441920/1‑150 (MQ=255)
                                         gCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGc                                                                                                              >  2:4872026/1‑150 (MQ=255)
                                               gACCCTGCGCATTCCGTTCGACGACACCCACGGCGCGCACCCCGCGCGCCCGGGCTTCTTACGGCTCTAGGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAAc                                                                                                        <  2:2146643/150‑1 (MQ=255)
                                                 cccTGCGCATTCGGTTCGACGACACCCTCGGCTCCCACCCCGCGCCCCGGGGCTTCTTCCGGCTATTGGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAAcgc                                                                                                      <  1:4640641/150‑1 (MQ=255)
                                                 cccTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGATTGTTACGGCTATATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAAcgc                                                                                                      <  2:2943757/150‑1 (MQ=255)
                                                      cgcATGCGGTTCGCCGACACCCCCGGCTCGCTCACCGCGCGCCCGGGCTTCTTAGGGATCTTGGCC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATg                                                                                                 <  1:1955798/150‑1 (MQ=255)
                                                        ctttCGGTTCGACGACCCCCTGGGCGCGCTCACCGGGGCCCGGGGCTTCTTCCGGCTCT‑TGGCAAACGACTCGCTCAATTCGACCTTCACCCACTCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGa                                                                                               <  1:2110381/148‑1 (MQ=255)
                                                        ctttCCGTTCGACGACCCCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGa                                                                                               <  2:2380806/148‑1 (MQ=255)
                                                        cATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGa                                                                                               >  2:942306/1‑150 (MQ=255)
                                                        atttCGGTTCGACGACCCCCGCGGCCGCCCCCCCGCGCGCCCGGTATTTTTCGGGATCTTGGCC‑AACGACTCGCTCAATTCCACCTACACCCACTCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGa                                                                                               <  1:2448286/148‑1 (MQ=255)
                                                           tCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGtt                                                                                            >  1:3262206/1‑150 (MQ=255)
                                                            cggTTCGACGACACCCTCGGCGCGCACACCGCGCGCCGGGGCTTATTCCGGCTATAGGCC‑AACGACTCGCTCAATTCGACCTCCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTtc                                                                                           <  1:4703118/148‑1 (MQ=255)
                                                            ccGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTtc                                                                                           >  1:2535349/1‑150 (MQ=255)
                                                              gtccgccgacACCCTCGGCTCGCTCACCGCGCGCCCGGGATTCTTCCGGCTCTAGGGC‑AACGACTCGCTCAATTCGACCTACACCCACTCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTtctc                                                                                         <  2:463815/144‑1 (MQ=255)
                                                              gTTCGACGACACCCTCGGCTCGCTCCCCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTACACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTtctc                                                                                         <  1:2384096/150‑1 (MQ=255)
                                                                 cgacgaCACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGcc                                                                                      >  1:159786/1‑150 (MQ=255)
                                                                 cgacgaCACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGcc                                                                                      >  1:1602689/1‑150 (MQ=255)
                                                                  gcccacacCCGGGGCCCGCTCCCCGCGCGCCGGGGATTGTTACGGCTATTTGCC‑AACGACTCGCTCAATCCGACCTTCACCCACTCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCg                                                                                     <  1:2882445/146‑1 (MQ=255)
                                                                    cgacACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGATCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCACTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGt                                                                                   <  2:3635014/150‑1 (MQ=255)
                                                                      acacCCGCGGCTCGCTCACCGCGCGCCCGGGCTTCTTCCGGCTCTAGGGC‑ACCGACTCGCTCAATTCGACCTTCACCCACTCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGc                                                                                 <  2:2318454/150‑1 (MQ=255)
                                                                       cacCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCa                                                                                <  2:2697552/150‑1 (MQ=255)
                                                                        acCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCAc                                                                               >  1:2736849/1‑150 (MQ=255)
                                                                         cccTCGGCTCGCTCACCGCGCGCCCGGGCTTGTTACGGCTCTAGGGC‑AACGACTCGCTCAATTCGACCTTCACCCACTCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACt                                                                              <  2:3481657/150‑1 (MQ=255)
                                                                         cccTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACt                                                                              <  2:1389471/150‑1 (MQ=255)
                                                                            tCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTcc                                                                           >  1:712498/1‑150 (MQ=255)
                                                                            tCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTcc                                                                           >  2:3855272/1‑150 (MQ=255)
                                                                             cGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCca                                                                          >  2:1024299/1‑150 (MQ=255)
                                                                             cGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCca                                                                          >  1:268698/1‑150 (MQ=255)
                                                                              ggCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCcag                                                                         >  2:1610208/1‑150 (MQ=255)
                                                                               gctcgctcCCCGCGGGCCGGGGCTTCTACGGGCTCTTGGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCcagc                                                                        <  1:4248398/150‑1 (MQ=255)
                                                                                 tcgctcACCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCcagcag                                                                      <  2:1561041/150‑1 (MQ=255)
                                                                                  cgcaccccGCGCGCCGGGGCTTCTTCGGGCTCTTGGGC‑AACGACTCGCTCAATTCGACCTTCACCCACTCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCaga                                                                     <  1:104862/144‑1 (MQ=255)
                                                                                    ctcACCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCAGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAgc                                                                   >  1:845727/1‑150 (MQ=255)
                                                                                     tcACCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAgcg                                                                  >  1:636675/1‑150 (MQ=255)
                                                                                     tcACCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAgcg                                                                  >  1:2998738/1‑150 (MQ=255)
                                                                                     accccGCGCGCCCGGGCTTCTTACGGCTCTAGGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAgcg                                                                  <  1:1925881/147‑1 (MQ=255)
                                                                                      cACCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCCGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAgcgc                                                                 >  2:546080/1‑150 (MQ=255)
                                                                                      cACCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAgcgc                                                                 >  1:948755/1‑150 (MQ=255)
                                                                                       cccGCGCGCCGGGGTTTCTTCCGGCTCT‑TGGCAAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGcgcg                                                                <  1:3827469/149‑1 (MQ=255)
                                                                                         cgcgcgCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCggg                                                              <  1:3616034/150‑1 (MQ=255)
                                                                                         cgcgcgCCCGGGCTTATTCCGGCTCTTTGGC‑AACGACTCGCTCAATTCGACCTTCACCCACTCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCggg                                                              <  1:4463782/150‑1 (MQ=255)
                                                                                             cgcCGGGGCTTCTTAGGGCTCTTGGCC‑AACGACTCGCTCAATTCGACCTTCACCCACTCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTg                                                          <  1:1082239/150‑1 (MQ=255)
                                                                                             cgcCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTg                                                          <  2:2766440/150‑1 (MQ=255)
                                                                                             cgcCCGGGCTTCTACCGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTg                                                          <  2:112552/150‑1 (MQ=255)
                                                                                                ccGGGCTTGTTCCGGCCCTATGGA‑AACGACTCGACCACTTCGACCTCCACCCACTCGACCGTGGCGAGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGAcc                                                       <  1:745000/150‑1 (MQ=255)
                                                                                                ccGGGCTTCTTACGGCTATTGGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGAcc                                                       <  1:4732984/150‑1 (MQ=255)
                                                                                                  gggCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTg                                                     >  2:3464405/1‑150 (MQ=255)
                                                                                                    gCTTCTTCCGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGct                                                   <  2:2402848/150‑1 (MQ=255)
                                                                                                    gCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGct                                                   >  2:2577796/1‑150 (MQ=255)
                                                                                                    gCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGct                                                   >  1:1633214/1‑150 (MQ=255)
                                                                                                     cttcttCCGGGTCTAGGGC‑AACGCCGCGCTCAATTCGACCTTCACCCACTCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGcta                                                  <  2:4145622/150‑1 (MQ=255)
                                                                                                     cttcttACGGCTCTAGGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGcta                                                  <  2:3124245/150‑1 (MQ=255)
                                                                                                     cttcttACGGCTCTAGGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGcta                                                  <  2:4024712/150‑1 (MQ=255)
                                                                                                      ttcttcCGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCACTCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGctac                                                 <  1:4423103/150‑1 (MQ=255)
                                                                                                       tcttACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGctact                                                <  1:1878259/150‑1 (MQ=255)
                                                                                                        tttccGGCTCTTGGCC‑AACGACTCGCTCAATTCGACCTTCACCCACTCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGctacta                                               <  1:4076649/146‑1 (MQ=255)
                                                                                                        cttACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCACTCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGctacta                                               <  1:3437079/150‑1 (MQ=255)
                                                                                                              gCTCTATGGA‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAg                                         <  2:3700227/150‑1 (MQ=255)
                                                                                                                 ctATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCaa                                      >  1:301387/1‑150 (MQ=255)
                                                                                                                   aTGGC‑AACGACTCGCTCAATTCGACCTTCACCCACTCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCaaaa                                    <  2:1986966/150‑1 (MQ=255)
                                                                                                                    tGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCaaaaa                                   >  2:1652906/1‑150 (MQ=255)
                                                                                                                       c‑AACGACTCGCTCAATTCGACCTTCACCCACTCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTg                                <  1:1260288/150‑1 (MQ=255)
                                                                                                                       c‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTg                                <  2:636675/150‑1 (MQ=255)
                                                                                                                        aaaCGACTCGCTCAATTCGACCTTCACCCACTCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTg                                <  1:4309572/149‑1 (MQ=255)
                                                                                                                           cGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAg                             >  2:4683874/1‑150 (MQ=255)
                                                                                                                              cTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTa                          <  1:3096729/150‑1 (MQ=255)
                                                                                                                              cTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTa                          <  1:176201/150‑1 (MQ=255)
                                                                                                                              cTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTa                          <  2:988242/150‑1 (MQ=255)
                                                                                                                              cTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTa                          >  1:1218291/1‑150 (MQ=255)
                                                                                                                               tCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTAc                         >  2:2953110/1‑150 (MQ=255)
                                                                                                                                cGCTCATTTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACt                        <  1:3148053/150‑1 (MQ=255)
                                                                                                                                 gCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTg                       >  1:4286961/1‑150 (MQ=255)
                                                                                                                                    cAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCtt                    >  2:1847562/1‑150 (MQ=255)
                                                                                                                                     aaTTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCttt                   <  2:2246360/150‑1 (MQ=255)
                                                                                                                                      tttCGACCTCCACCCACTCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTtg                  <  1:3701842/149‑1 (MQ=255)
                                                                                                                                      aTTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTtg                  <  2:3262206/150‑1 (MQ=255)
                                                                                                                                         cGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTgg               >  2:589466/1‑150 (MQ=255)
                                                                                                                                          gACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGa              <  2:432876/150‑1 (MQ=255)
                                                                                                                                               tCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACg         >  1:3876454/1‑150 (MQ=255)
                                                                                                                                                cACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACga        >  2:2105046/1‑150 (MQ=255)
                                                                                                                                                    cAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACgaggag    >  2:1238642/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
AACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGC‑AACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGG  >  CP009273/283487‑283785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑