Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,908,261 T→A 20.2% L100F (TTA→TTT)  glmU ← fused N‑acetyl glucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,908,2610TA20.2% 756.2 / 198.5 307L100F (TTA→TTT) glmUfused N‑acetyl glucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyl transferase
Reads supporting (aligned to +/- strand):  ref base T (113/132);  new base A (30/32);  total (143/164)
Fisher's exact test for biased strand distribution p-value = 7.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.86e-01

Only 100 of 310 total aligned reads displayed.

GTAACTTTGCCGTTTTCACGGGTGATACGTCCATAACCGGTCGGATCATCCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTTCAGCGCCTGTTTTAGCAGATCGCCGCCGTGACCGTACACCAGGTGA  >  CP009273/3908112‑3908408
                                                                                                                                                     |                                                                                                                                                   
gTAACTTTGCCGTTTTCACGGGTGATACGTCCATAACCGGTCGGATCATCCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCAtt                                                                                                                                                     <  1:3614868/150‑1 (MQ=255)
 tAACTTTGCCGTTTTCACGGGTGATACGTCCATAACCGGTCGGATCATCCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTa                                                                                                                                                    >  2:604044/1‑150 (MQ=255)
   aCTTTGCCGTTTTCACGGGTGATACGTCCATAACCGGTCGGATCATCCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATaaaa                                                                                                                                                  <  2:1613299/150‑1 (MQ=255)
       tGCCGTTTTCACGGGTGATACGTCCATAACCGGTCGGATCATCCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGt                                                                                                                                              >  2:4039470/1‑150 (MQ=255)
        gCCGTTTTCACGGGTGATACGTCCATAACCGGTCGGATCATCCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTc                                                                                                                                             <  2:1224733/150‑1 (MQ=255)
        gCCGTTTTCACGGGTGATACGTCCATAACCGGTCGGATCATCCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTc                                                                                                                                             >  1:1211753/1‑150 (MQ=255)
        gCCGTTTTCACGGGTGATACGTCCATAACCGGTCGGATCATCCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTc                                                                                                                                             <  2:1109511/150‑1 (MQ=255)
          cGTTTTCACGGGTGATACGTCCATAACCGGTCGGATCATCCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATAAAAATGTCtt                                                                                                                                           <  1:2879237/150‑1 (MQ=255)
           gTTTTCACGGGTGATACGTCCATAACCGGTCGGATCATCCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTtc                                                                                                                                          <  1:5576/150‑1 (MQ=255)
           gTTTTCACGGGTGATACGTCCATAACCGGTCGGATCATCCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATAAAAATGTCTtc                                                                                                                                          <  1:3222340/150‑1 (MQ=255)
              ttCACGGGTGATACGTCCATAACCGGTCGGATCATCCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTtcatc                                                                                                                                       <  2:1954164/150‑1 (MQ=255)
                 aCGGGTGATACGTCCATAACCGGTCGGATCATCCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCatcatc                                                                                                                                    >  1:3388452/1‑150 (MQ=255)
                 aCGGGTGATACGTCCATAACCGGTCGGATCATCCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCatcatc                                                                                                                                    <  2:2214716/150‑1 (MQ=255)
                   gggTGATACGTCCATAACCGGTCGGATCATCCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCgg                                                                                                                                  <  1:1708713/150‑1 (MQ=255)
                   gggTGATACGTCCATAACCGGTCGGATCATCCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCgg                                                                                                                                  >  1:3265695/1‑150 (MQ=255)
                   gggTGATACGTCCATAACCGGTCGGATCATCCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCgg                                                                                                                                  >  2:1983672/1‑150 (MQ=255)
                   gggTGATACGTCCATAACCGGTCGGATCATCCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATAAAAATGTCTTCATCATCgg                                                                                                                                  <  2:586316/150‑1 (MQ=255)
                        aTACGTCCATAACCGGTCGGATCATCCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCaaag                                                                                                                             >  1:4421002/1‑150 (MQ=255)
                          aCGTCCATAACCGGTCGGATCATCCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCaaagaa                                                                                                                           <  2:3500925/150‑1 (MQ=255)
                           cGTCCATAACCGGTCGGATCATCCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCaaagaaa                                                                                                                          >  2:164900/1‑150 (MQ=255)
                              ccATAACCGGTCGGATCATCCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGt                                                                                                                       >  2:429858/1‑150 (MQ=255)
                               cATAACCGGTCGGATCATCCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTg                                                                                                                      >  2:701033/1‑150 (MQ=255)
                               cATAACCGGTCGGATCATCCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTg                                                                                                                      <  2:2474486/150‑1 (MQ=255)
                               cATAACCGGTCGGATCATCCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTg                                                                                                                      >  2:3130550/1‑150 (MQ=255)
                               cATAACCGGTCGGATCATCCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATAAAAATGTCTTCATCATCGGCAAAGAAAGGTg                                                                                                                      <  1:537311/150‑1 (MQ=255)
                                aTAACCGGTCGGATCATCCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATAAAAATGTCTTCATCATCGGCAAAGAAAGGTGc                                                                                                                     >  1:2703973/1‑150 (MQ=255)
                                aTAACCGGTCGGATCATCCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATAAAAATGTCTTCATCATCGGCAAAGAAAGGTGc                                                                                                                     >  1:1737487/1‑150 (MQ=255)
                                 tAACCGGTCGGATCATCCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATAAAAATGTCTTCATCATCGGCAAAGAAAGGTGCg                                                                                                                    >  2:3997204/1‑150 (MQ=255)
                                    ccGGTCGGATCATCCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGcc                                                                                                                 >  2:1249946/1‑150 (MQ=255)
                                     cGGTCGGATCATCCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCct                                                                                                                <  1:113325/150‑1 (MQ=255)
                                       gTCGGATCATCCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCctgc                                                                                                              <  1:789764/150‑1 (MQ=255)
                                        tCGGATCATCCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCctgct                                                                                                             >  1:1072546/1‑150 (MQ=255)
                                         cGGATCATCCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCctgctg                                                                                                            >  1:1923865/1‑150 (MQ=255)
                                         cGGATCATCCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCctgctg                                                                                                            <  1:948675/150‑1 (MQ=255)
                                         cGGATCATCCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCctgctg                                                                                                            >  1:4485858/1‑150 (MQ=255)
                                           gATCATCCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCtgca                                                                                                          <  1:1361628/150‑1 (MQ=255)
                                             tcatcCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCtgcatt                                                                                                        <  2:4244021/150‑1 (MQ=255)
                                             tcatcCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATAAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCtgcatt                                                                                                        <  2:3075855/150‑1 (MQ=255)
                                             tcatcCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATAAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCtgcatt                                                                                                        <  2:643485/150‑1 (MQ=255)
                                               atcCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCCAAGAAAGGTGCGGCCTGCtgcattgc                                                                                                      >  1:3280115/1‑150 (MQ=255)
                                                tcCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCtgcattgca                                                                                                     <  1:3452209/150‑1 (MQ=255)
                                                 ccAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATAAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCtgcattgcat                                                                                                    <  1:2811850/150‑1 (MQ=255)
                                                  cAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATg                                                                                                   >  1:3102225/1‑150 (MQ=255)
                                                   aGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGa                                                                                                  <  1:2524859/150‑1 (MQ=255)
                                                    gTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGAc                                                                                                 <  2:3661075/150‑1 (MQ=255)
                                                       tCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATAAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCg                                                                                              <  2:2110794/150‑1 (MQ=255)
                                                          ccGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATAAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTAc                                                                                           >  1:3674488/1‑150 (MQ=255)
                                                             tCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCa                                                                                        <  2:1805306/150‑1 (MQ=255)
                                                              cagcagACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAg                                                                                       >  1:3185418/1‑150 (MQ=255)
                                                              cagcagACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATAAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAg                                                                                       <  2:4135416/150‑1 (MQ=255)
                                                                gcagACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAgct                                                                                     <  1:1038679/150‑1 (MQ=255)
                                                                    aCCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGctc                                                                                 <  2:1377744/150‑1 (MQ=255)
                                                                      cAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATAAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTg                                                                               <  2:3778605/150‑1 (MQ=255)
                                                                        aTGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGcc                                                                             >  2:1680077/1‑150 (MQ=255)
                                                                         tGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATAAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCt                                                                            <  2:1897638/150‑1 (MQ=255)
                                                                          gCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTg                                                                           <  2:1016868/150‑1 (MQ=255)
                                                                          gCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTg                                                                           <  2:3388452/150‑1 (MQ=255)
                                                                           ccACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGa                                                                          <  2:2083938/150‑1 (MQ=255)
                                                                            cACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGaa                                                                         <  1:2014865/150‑1 (MQ=255)
                                                                            cACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGaa                                                                         >  2:4061000/1‑150 (MQ=255)
                                                                              cccTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGc                                                                       >  2:826025/1‑150 (MQ=255)
                                                                              cccTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGc                                                                       >  2:3702742/1‑150 (MQ=255)
                                                                                 tGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCAcc                                                                    <  1:4138355/150‑1 (MQ=255)
                                                                                 tGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATAAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCAcc                                                                    >  1:3785774/1‑150 (MQ=255)
                                                                                   cGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCa                                                                  <  1:2618321/150‑1 (MQ=255)
                                                                                   cGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCa                                                                  <  1:2137266/150‑1 (MQ=255)
                                                                                        gaGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATAAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTaa                                                             <  2:3035511/149‑1 (MQ=255)
                                                                                          gCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAgg                                                           <  1:4549278/150‑1 (MQ=255)
                                                                                          gCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAgg                                                           >  2:2393876/1‑150 (MQ=255)
                                                                                           cATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGt                                                          <  1:3479923/150‑1 (MQ=255)
                                                                                           cATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGt                                                          >  1:4304957/1‑150 (MQ=255)
                                                                                             tCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTg                                                        <  2:4454615/150‑1 (MQ=255)
                                                                                              cACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATAAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTgt                                                       >  2:2935806/1‑150 (MQ=255)
                                                                                                cGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTgtcg                                                     <  2:2785159/150‑1 (MQ=255)
                                                                                                 gCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTgtcgt                                                    <  2:2276308/150‑1 (MQ=255)
                                                                                                 gCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTgtcgt                                                    <  2:2872896/150‑1 (MQ=255)
                                                                                                    gACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCtt                                                 <  2:4839255/150‑1 (MQ=255)
                                                                                                    gACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCtt                                                 <  1:604040/150‑1 (MQ=255)
                                                                                                    gACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCtt                                                 <  2:532794/150‑1 (MQ=255)
                                                                                                      cGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTTc                                               <  1:3700835/150‑1 (MQ=255)
                                                                                                         tGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTTCAgc                                            >  2:3610312/1‑150 (MQ=255)
                                                                                                         tGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATAAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTTCAgc                                            >  1:3194246/1‑150 (MQ=255)
                                                                                                          ggAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATAAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTTCAgcg                                           <  1:4108097/150‑1 (MQ=255)
                                                                                                            aGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTTCAGCGcc                                         <  1:3990496/150‑1 (MQ=255)
                                                                                                             gtgtTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTTCAGCGCCt                                        <  1:1541276/150‑1 (MQ=255)
                                                                                                             gtgtTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTTCAGCGCCt                                        <  1:2749411/150‑1 (MQ=255)
                                                                                                                tttCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTTCAGCGCCTGtt                                     <  2:2629091/150‑1 (MQ=255)
                                                                                                                   cGACAGAGATCAGCGGCACGTCGCCGTAGAGCATAAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTTCAGCGCCTGTTTTa                                  >  2:1336918/1‑150 (MQ=255)
                                                                                                                       agagATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTTCAGCGCCTGTTTTAGCAg                              >  2:2137307/1‑150 (MQ=255)
                                                                                                                         agaTCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTTCAGCGCCTGTTTTAGCAGAt                            <  1:1297231/150‑1 (MQ=255)
                                                                                                                             cAGCGGCACGTCGCCGTAGAGCATAAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTTCAGCGCCTGTTTTAGCAGATcgcc                        >  2:391922/1‑150 (MQ=255)
                                                                                                                                   cACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTTCAGCGCCTGTTTTAGCAGATCGCCGCCGTg                  <  1:3145208/150‑1 (MQ=255)
                                                                                                                                    aCGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGGTGTCGTCTTTCAGCGCCTGTTTTAGCAGATCGCCGCCGTGa                 <  2:4574694/150‑1 (MQ=255)
                                                                                                                                     cGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTTCAGCGCCTGTTTTAGCAGATCGCCGCCGTGAc                <  1:1522320/150‑1 (MQ=255)
                                                                                                                                      gTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTTCAGCGCCTGTTTTAGCAGATCGCCGCCGTGAcc               >  1:4227732/1‑150 (MQ=255)
                                                                                                                                      gTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTTCAGCGCCTGTTTTAGCAGATCGCCGCCGTGAcc               >  1:2081622/1‑150 (MQ=255)
                                                                                                                                           cGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTTCAGCGCCTGTTTTAGCAGATCGCCGCCGTGACCGTaca          >  1:4388886/1‑150 (MQ=255)
                                                                                                                                            gTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTTCAGCGCCTGTTTTAGCAGATCGCCGCCGTGACCGTacac         <  2:909441/150‑1 (MQ=255)
                                                                                                                                              agagCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTTCAGCGCCTGTTTTAGCAGATCGCCGCCGTGACCGTACACCa       >  2:3202444/1‑150 (MQ=255)
                                                                                                                                                agCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTTCAGCGCCTGTTTTAGCAGATCGCCGCCGTGACCGTACACCAgg     <  1:636921/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GTAACTTTGCCGTTTTCACGGGTGATACGTCCATAACCGGTCGGATCATCCAGTTTCACCGTCAGCAGACCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTTCAGCGCCTGTTTTAGCAGATCGCCGCCGTGACCGTACACCAGGTGA  >  CP009273/3908112‑3908408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑