BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,926,496	A→C	28.0%	E607D (GAA→GAC)	kup →	potassium transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,926,496	0	A	C	28.0%	422.1 / 271.1	279	E607D (GAA→GAC)	kup	potassium transporter
Reads supporting (aligned to +/- strand): ref base A (86/115); new base C (27/51); total (113/166)
Fisher's exact test for biased strand distribution p-value = 2.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

Only 100 of 285 total aligned reads displayed.

AAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGAT  >  CP009273/3926348‑3926645

aaGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTTATCCTCGGCAAAAGCCCGTGGTATTTTCGTCTGCGCGGCAAGCAGTACTTGCTGCTGCAAAGTAATGCGCTGCGTGCACCAGATCAATTTGaaa                                                                                                                                                      >  2:423817/1‑150 (MQ=255)

aaGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGaaa                                                                                                                                                      >  2:1261684/1‑150 (MQ=255)

 aGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAAt                                                                                                                                                     >  1:1538263/1‑150 (MQ=255)

  ggATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATc                                                                                                                                                    >  2:1123348/1‑150 (MQ=255)

  ggATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATc                                                                                                                                                    >  2:2657187/1‑150 (MQ=255)

    aTTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATccc                                                                                                                                                  <  2:1911921/150‑1 (MQ=255)

      tAAGTTGCCGGATGATGGAAACCTACTTCTTTATGTCGCATGAGTCGTTGATCCACGGCAAACTCACGTGGTATTTGAGTCTGCGCGGCAAGCTGTACTAGATGCTGCAACGTAATGCGCTGAGTGAAACAGATCAATTTGAAAtaacgc                                                                                                                                                >  2:3889931/1‑145 (MQ=255)

         gTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGACATCCCGCCaa                                                                                                                                             <  1:4553630/150‑1 (MQ=255)

         gTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGACATCCCGCCaa                                                                                                                                             <  1:2451813/150‑1 (MQ=255)

         gTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCaa                                                                                                                                             <  1:541700/150‑1 (MQ=255)

              cGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGACATCCCGCCAAACAgg                                                                                                                                        >  1:1491579/1‑150 (MQ=255)

              cGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGGAATGCGCTGCGTGCACCAGATCAATTTGACATCCCGCCAAACAgg                                                                                                                                        >  1:4328330/1‑150 (MQ=255)

                gatgatGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGACATCCCGCCAAACAGGGt                                                                                                                                      >  2:4128936/1‑150 (MQ=255)

                 atgatgGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGtt                                                                                                                                     <  1:250609/150‑1 (MQ=255)

                 atgatgGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCAGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGtt                                                                                                                                     >  1:1035769/1‑150 (MQ=255)

                  tgatgGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTa                                                                                                                                    <  1:2185030/150‑1 (MQ=255)

                     tgGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCg                                                                                                                                 <  2:3347710/150‑1 (MQ=255)

                     tgGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCg                                                                                                                                 >  1:611989/1‑150 (MQ=255)

                     tgGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCg                                                                                                                                 >  1:3609007/1‑150 (MQ=255)

                     tgGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCg                                                                                                                                 <  1:3553481/150‑1 (MQ=255)

                        aaaCCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAAc                                                                                                                              <  2:4323918/150‑1 (MQ=255)

                        aaaCCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAAc                                                                                                                              <  2:535784/150‑1 (MQ=255)

                         aaCCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACt                                                                                                                             <  1:3981875/150‑1 (MQ=255)

                          aCCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTg                                                                                                                            <  2:3677544/150‑1 (MQ=255)

                            ctcctTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATTCGCTGCGTGCACCCGAACAATTTGACATACCGCCAAAAAGGGTTATCGAACTggg                                                                                                                          >  1:2509721/1‑150 (MQ=255)

                            ctcctTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTggg                                                                                                                          <  2:106896/150‑1 (MQ=255)

                             tcctTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCAGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGt                                                                                                                         >  2:4126503/1‑150 (MQ=255)

                              cctTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTa                                                                                                                        <  2:3555166/150‑1 (MQ=255)

                                ttcttTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGACATCCCGCCAAACAGGGTTATCGAACTGGGTACt                                                                                                                      <  2:597636/150‑1 (MQ=255)

                                 tcttTATGTCGCATGAGTCGTTGATCCTCGGAAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTc                                                                                                                     <  1:4082366/150‑1 (MQ=255)

                                    ttATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAgg                                                                                                                  <  2:2977524/150‑1 (MQ=255)

                                     tATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGACATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGt                                                                                                                 <  2:1832214/150‑1 (MQ=255)

                                     tATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGt                                                                                                                 >  2:3534035/1‑150 (MQ=255)

                                       tGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGACATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCg                                                                                                               >  2:3093676/1‑150 (MQ=255)

                                       tGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGGGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCg                                                                                                               >  2:736195/1‑150 (MQ=255)

                                         tCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGaa                                                                                                             <  2:1791584/150‑1 (MQ=255)

                                         tCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGaa                                                                                                             <  1:501393/150‑1 (MQ=255)

                                              tGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTAATTGCTTCTGCAACGTAAAGCGCTGCGTGCACCACATAAAATTTAAATACCGCCAAACAGGGTTATAGAACAGGGTACTCAGGTCGAAATCTa                                                                                                        >  2:10330/1‑150 (MQ=255)

                                               gAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTaa                                                                                                       <  1:3143153/150‑1 (MQ=255)

                                               gAGTCGTTGATCCACGGCAAAAGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATTCGCTGCGTGCCCCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTaa                                                                                                       >  2:357111/1‑150 (MQ=255)

                                                aGTCGTTGATCCTCGGCACACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAAc                                                                                                      <  2:405535/150‑1 (MQ=255)

                                                aGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGACATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAAc                                                                                                      <  1:4377723/150‑1 (MQ=255)

                                                aGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAAc                                                                                                      >  2:4647050/1‑150 (MQ=255)

                                                aGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAAc                                                                                                      >  2:2197188/1‑150 (MQ=255)

                                                aGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAAc                                                                                                      <  1:2345415/150‑1 (MQ=255)

                                                      tGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTCCTCAGGTCGAAATCTAACGCCAGa                                                                                                >  1:2074256/1‑150 (MQ=255)

                                                       gATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGACATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGAc                                                                                               >  1:2765684/1‑150 (MQ=255)

                                                        aTCCTGGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACg                                                                                              <  1:3855970/150‑1 (MQ=255)

                                                          ccTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGcc                                                                                            >  2:2199050/1‑150 (MQ=255)

                                                            tCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGcctc                                                                                          >  1:3289800/1‑150 (MQ=255)

                                                              ggCACACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGcctcct                                                                                        <  2:894901/150‑1 (MQ=255)

                                                               gCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGACATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCtt                                                                                       <  1:175169/150‑1 (MQ=255)

                                                                  aaCGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCAACAGATCAATTTGACATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTttct                                                                                    <  1:739326/150‑1 (MQ=255)

                                                                    cGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTttcttc                                                                                  <  1:2960617/150‑1 (MQ=255)

                                                                     gCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGACATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCa                                                                                 <  2:127406/150‑1 (MQ=255)

                                                                     gCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGACATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCa                                                                                 <  2:2042663/150‑1 (MQ=255)

                                                                      cccGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGACATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCAt                                                                                <  1:3734724/150‑1 (MQ=255)

                                                                      cccGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGACATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCAt                                                                                <  1:2867201/150‑1 (MQ=255)

                                                                         gTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAg                                                                             <  2:2356020/150‑1 (MQ=255)

                                                                         gTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAg                                                                             <  2:4617961/150‑1 (MQ=255)

                                                                           ggTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAggg                                                                           <  2:3112245/150‑1 (MQ=255)

                                                                           ggTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAggg                                                                           >  1:4232471/1‑150 (MQ=255)

                                                                              aTTTGCTTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGACATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGc                                                                        <  2:4725021/150‑1 (MQ=255)

                                                                                ttGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGt                                                                      >  2:4414343/1‑150 (MQ=255)

                                                                                ttGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGt                                                                      <  1:3906921/150‑1 (MQ=255)

                                                                                 tGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGtg                                                                     >  1:2320211/1‑149 (MQ=255)

                                                                                    gTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGACATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGttttt                                                                  <  2:1605385/150‑1 (MQ=255)

                                                                                    gTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGttttt                                                                  <  1:859037/150‑1 (MQ=255)

                                                                                         cgcgGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGtttt                                                             <  1:1520498/150‑1 (MQ=255)

                                                                                          gcgGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCTAGTTGAAATCCCGCCAACCAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATCAGGGGGCGTTTTTGTTTTc                                                            <  2:2878937/150‑1 (MQ=255)

                                                                                            ggCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCAt                                                          <  2:3173998/150‑1 (MQ=255)

                                                                                              cAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATgg                                                        <  1:4248671/150‑1 (MQ=255)

                                                                                               aaGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGACATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGt                                                       <  1:4775556/150‑1 (MQ=255)

                                                                                                  cTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTTTTTTCATTGTTaa                                                    >  1:3926176/1‑150 (MQ=255)

                                                                                                  cTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTaa                                                    >  1:2386503/1‑150 (MQ=255)

                                                                                                  cTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTaa                                                    >  1:3925154/1‑150 (MQ=255)

                                                                                                    gTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTTTTTTCATGGTTAAta                                                  >  1:2680114/1‑149 (MQ=255)

                                                                                                        ttGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCa                                              <  2:78412/150‑1 (MQ=255)

                                                                                                        ttGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCa                                              <  2:2511011/150‑1 (MQ=255)

                                                                                                         tgctgctgcAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCAt                                             <  1:10330/150‑1 (MQ=255)

                                                                                                         tgctgctgcAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCAt                                             >  2:283164/1‑150 (MQ=255)

                                                                                                                gcAACGTAATGCGCTGCGTGCACCAGATCAATTTGACATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAAc                                      <  1:31518/150‑1 (MQ=255)

                                                                                                                  aaCGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTGTTTGTTTTAATTGTTTATAACCATGTAAAAAGt                                    >  1:4623557/1‑150 (MQ=255)

                                                                                                                   aCGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGtt                                   <  1:212946/150‑1 (MQ=255)

                                                                                                                    cGTAATGCGCTGCGTGCACCAGATCAATTTGACATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGttt                                  <  2:2014429/150‑1 (MQ=255)

                                                                                                                     gTAATGCGCTGCGTGCACCAGATCAATTTGACATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTc                                 <  1:116146/150‑1 (MQ=255)

                                                                                                                     gTAATGCGCTGCGTGCACCAGATCAATTTGACATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTc                                 <  1:1301544/150‑1 (MQ=255)

                                                                                                                           tgcTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGtt                           >  2:4539194/2‑150 (MQ=255)

                                                                                                                           cgcTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATATAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATTTAAAACGTTTCGAGGtt                           <  1:3889931/150‑1 (MQ=255)

                                                                                                                             cggCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGa                         <  1:1252371/148‑1 (MQ=255)

                                                                                                                             cTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGa                         <  1:195920/150‑1 (MQ=255)

                                                                                                                                cGTGCACCAGATCAATTTGACATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATca                      <  2:1524651/150‑1 (MQ=255)

                                                                                                                                    cACCAGATCAATTTGACATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACAtt                  <  2:1491710/150‑1 (MQ=255)

                                                                                                                                     aCCAGATCAATTAGACATCACGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACTCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTAATTGTTAATAAACATTTAAAACCTTTCGATGTTGATAACAttt                 >  2:2125577/1‑150 (MQ=255)

                                                                                                                                       cAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTcc               <  1:4439878/150‑1 (MQ=255)

                                                                                                                                           tCAATTTGACATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTaa           >  2:3588239/1‑150 (MQ=255)

                                                                                                                                           tCAATTTGACATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTAATTGTTAATAACCATGTAAAACGTTTCGAGGTTGATAACATTTCCGTaa           >  2:1401167/1‑150 (MQ=255)

                                                                                                                                            cAATTTGACATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAAc          <  2:3144222/150‑1 (MQ=255)

                                                                                                                                               tttGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTc       >  2:1576758/1‑150 (MQ=255)

                                                                                                                                                    aaTCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGAt  >  1:1575825/1‑150 (MQ=255)

AAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGAT  >  CP009273/3926348‑3926645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`