Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,960,100 A→T 8.0% E108D (GAA→GAT)  rho → transcription termination factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,960,1000AT 8.0% 847.7 / 51.4 266E108D (GAA→GAT) rhotranscription termination factor
Reads supporting (aligned to +/- strand):  ref base A (125/117);  new base T (16/6);  total (141/125)
Fisher's exact test for biased strand distribution p-value = 7.39e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 271 total aligned reads displayed.

CAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTTAA  >  CP009273/3959951‑3960248
                                                                                                                                                     |                                                                                                                                                    
cAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGaa                                                                                                                                                      <  2:678940/150‑1 (MQ=255)
     tggatTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTa                                                                                                                                                 <  1:4715193/150‑1 (MQ=255)
      ggatTTGGGTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTAt                                                                                                                                                <  2:2915201/150‑1 (MQ=255)
         tttGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGATCGCTAtttt                                                                                                                                             >  2:3892153/1‑150 (MQ=255)
             gTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTgcgc                                                                                                                                         >  1:4137812/1‑150 (MQ=255)
             gTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTgcgc                                                                                                                                         >  1:1305372/1‑150 (MQ=255)
              tttCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCgct                                                                                                                                        >  1:2873080/1‑150 (MQ=255)
               ttCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCgctg                                                                                                                                       <  1:3751524/150‑1 (MQ=255)
                tcctccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCgctgc                                                                                                                                      >  2:795149/1‑150 (MQ=255)
                     cGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGaaa                                                                                                                                 <  1:1206371/150‑1 (MQ=255)
                     cGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGaaa                                                                                                                                 >  1:3783578/1‑150 (MQ=255)
                       ttCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGt                                                                                                                               <  2:4115646/150‑1 (MQ=255)
                       ttCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGt                                                                                                                               >  1:2044375/1‑150 (MQ=255)
                        tCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGtt                                                                                                                              <  2:1074074/150‑1 (MQ=255)
                        tCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGtt                                                                                                                              <  2:2818787/150‑1 (MQ=255)
                        tCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGtt                                                                                                                              <  2:2501976/150‑1 (MQ=255)
                        tCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGtt                                                                                                                              <  2:2279100/150‑1 (MQ=255)
                        tCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGtt                                                                                                                              >  1:1900025/1‑150 (MQ=255)
                        tCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCCCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGtt                                                                                                                              >  2:1244867/1‑150 (MQ=255)
                         ccGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTa                                                                                                                             <  1:4774668/150‑1 (MQ=255)
                          cGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTaa                                                                                                                            <  2:3495435/150‑1 (MQ=255)
                           gCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAAc                                                                                                                           >  1:1156031/1‑150 (MQ=255)
                            cAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGATCGCTATTTTGCGCTGCTGAAAGTTAACg                                                                                                                          <  1:1661165/150‑1 (MQ=255)
                            cAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACg                                                                                                                          <  1:3157168/150‑1 (MQ=255)
                               aCAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAg                                                                                                                       <  1:2799451/150‑1 (MQ=255)
                                cAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGt                                                                                                                      >  1:1370868/1‑150 (MQ=255)
                                   cTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTaa                                                                                                                   >  1:1025116/1‑150 (MQ=255)
                                    tCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAAc                                                                                                                  <  2:4828092/150‑1 (MQ=255)
                                    tCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAAc                                                                                                                  >  2:3392133/1‑150 (MQ=255)
                                     ccTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACt                                                                                                                 <  2:4654032/150‑1 (MQ=255)
                                     ccTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACt                                                                                                                 <  2:2038217/150‑1 (MQ=255)
                                       tACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTc                                                                                                               <  2:4166421/150‑1 (MQ=255)
                                       tACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTc                                                                                                               <  2:4531084/150‑1 (MQ=255)
                                       tACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTc                                                                                                               <  2:1252219/150‑1 (MQ=255)
                                            cGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACaa                                                                                                          <  2:3238988/150‑1 (MQ=255)
                                            cGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACaa                                                                                                          >  2:1354571/1‑150 (MQ=255)
                                                 gTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTg                                                                                                     <  1:4578115/150‑1 (MQ=255)
                                                 gTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTg                                                                                                     >  2:2427306/1‑150 (MQ=255)
                                                 gTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTg                                                                                                     <  1:2124973/150‑1 (MQ=255)
                                                 gTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTg                                                                                                     >  1:4527861/1‑150 (MQ=255)
                                                   ccTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGaa                                                                                                   <  2:2773121/150‑1 (MQ=255)
                                                     tgatgaCATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGaaaa                                                                                                 <  1:3566304/150‑1 (MQ=255)
                                                      gatgaCATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAAc                                                                                                <  1:3843231/150‑1 (MQ=255)
                                                      gatgaCATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAAc                                                                                                >  1:2385998/1‑150 (MQ=255)
                                                      gatgaCATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAAc                                                                                                <  1:1382773/150‑1 (MQ=255)
                                                            aTCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGATCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGc                                                                                          >  1:983926/1‑150 (MQ=255)
                                                                aCGTTTCCCCTCGCCAATTCCACGGTTTAACCCTCCGCAGTGGTGATACCATCTGTGGTAAGATTCGCCCGCCGAAATATGGTTAACGCTATTTTGCGGTGCTGATATTTAACGATGTTAAATTCTACAAACCTGAAAACGCCCGcaaca                                                                                      <  2:1021805/150‑1 (MQ=255)
                                                                aCGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTTATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTTAAATTTAACTAAGTTAACTTCGACAAACCTGAAAACGCCCGcaaca                                                                                      <  2:4722258/150‑1 (MQ=255)
                                                                    ttCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAAt                                                                                  >  2:1871125/1‑150 (MQ=255)
                                                                      ccccTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATcc                                                                                >  1:3007312/1‑150 (MQ=255)
                                                                      ccccTAGCCAAACCCGCCTTTTCAACCTCCGAACTGTTGATACAATCTCTGGTAATATTCGCCCGCCTAAATAAGGTGAACGATATTTTGCGCTGCTTAATTTTAACTAATTTAACTTCGAAAAACCTGAAAACGCCCGCAACAAAATcc                                                                                <  2:881237/150‑1 (MQ=255)
                                                                      ccccTAGCAAAATCCACCTTTTCAACCTCCGCAGTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGATGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATcc                                                                                <  2:3043656/150‑1 (MQ=255)
                                                                        ccTAGCCAAATCCACCTTTTCAACCTCCGCACTGGTGATACCTTCTCTGTTAAGATTCGCCCGCCTAAAGACGGTGATCGCTATTTTGCGCTGCTGAAATTTAACGATGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCctc                                                                              <  2:490253/150‑1 (MQ=255)
                                                                         cTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCctct                                                                             >  1:3940203/1‑150 (MQ=255)
                                                                          tAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCtt                                                                            >  1:1779043/1‑150 (MQ=255)
                                                                             ccAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTga                                                                         <  2:3616396/150‑1 (MQ=255)
                                                                                 aTCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAAc                                                                     <  2:2675192/150‑1 (MQ=255)
                                                                                 aTCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCATCTTTGAGAAc                                                                     >  1:3477684/1‑150 (MQ=255)
                                                                                 aTCCACCGTTTCAACCGCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACTAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAAc                                                                     <  2:578876/150‑1 (MQ=255)
                                                                                  tCCACCGTTTAAACCTCCGCACTGGTGATACCATCTCTGGTATGTTTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGTAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACt                                                                    <  2:1280522/150‑1 (MQ=255)
                                                                                     acctttacAACCTCCGCATTGTTTATACCATCTCTGTTAAGATGCGCCCGCCTAAAGTTGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGATTTTAACTTCGACAAACCTCAACACGCCCGCAACAAAATCCTCTTTGAGAACTTaa                                                                 <  1:2461033/143‑1 (MQ=255)
                                                                                      ccgTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCGTTGAGAACTTAAc                                                                >  2:2048948/1‑150 (MQ=255)
                                                                                       cgTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCAGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAAca                                                               >  2:2392973/1‑149 (MQ=255)
                                                                                         ttacAACCTCCGCTCTGTTTATACCATCTCTGTTAATTTTCGCCCGCCTAAATATGGTGAACGATTTTTTGCGCTGATTAAATTTAACTAATTTAACTTCGACAAACCTCAACACGCCCGCAACAAAATCCTCTTTGAGAACTTAAcccc                                                             <  1:4162698/147‑1 (MQ=255)
                                                                                            cAACCTCCGCACTGTTGATACCATCTCTGGTAAGATTCGCCCGCCTAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCt                                                          <  2:1696574/150‑1 (MQ=255)
                                                                                             aaCCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTg                                                         >  1:3607948/1‑150 (MQ=255)
                                                                                              aCCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGATCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGc                                                        >  1:4039122/1‑150 (MQ=255)
                                                                                              aCCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGc                                                        >  2:2361337/1‑150 (MQ=255)
                                                                                                 tCCGCTCTGGTGATACCTTCTCTGGTAAGATTCGCCCGCCTAAAGAAGGTGAACGGTATTTTGCGCTGCTTAATGTTAACGATGTTAACTTCTAAAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACg                                                     <  1:1947858/150‑1 (MQ=255)
                                                                                                 tCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACg                                                     <  2:2610931/150‑1 (MQ=255)
                                                                                                  ccGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGATCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGc                                                    >  1:890440/1‑150 (MQ=255)
                                                                                                      tcTGGTTATACCATCTCTGTTAAGTTTCGCCCGCCGAAAGATGGTGAACGCTATTTTGCGCTGCTTAAATTTAACTAAGTTAACTTCGACAAACCTGAACACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAAc                                                <  1:4241173/149‑1 (MQ=255)
                                                                                                      aCTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAAc                                                >  2:495965/1‑150 (MQ=255)
                                                                                                        tGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAActc                                              >  2:2044746/1‑150 (MQ=255)
                                                                                                         gtttaTACAATCTCTGTTATTATTCGCCCGCCGAACTATGTTGAACGCTATTTTGCGCTGCTTAATTTTAACGAAGTTAACTTCGACAACCCGCAACACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAActct                                             <  1:2951706/146‑1 (MQ=255)
                                                                                                          gTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACtctc                                            >  2:2259611/1‑150 (MQ=255)
                                                                                                           tGATACCTTCTCTGGTAAGATTCGCCGGCCTAAAGAAGGTTAACGCTATTTTGCGCTGCTTAATGTTAACGAAGTTAACTTCTACAAACCTTAAAACGCCCTCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCg                                           <  2:4321600/150‑1 (MQ=255)
                                                                                                           tGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGATATTTTGCGCTGCTGAATGTTAACGAATTTAACTTCGAAAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCg                                           <  1:2339453/150‑1 (MQ=255)
                                                                                                            gATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAATTTAACGATGTTAACTTCGAAAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGt                                          <  1:4854166/150‑1 (MQ=255)
                                                                                                            gATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGt                                          >  1:2780148/1‑150 (MQ=255)
                                                                                                                ccATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGc                                      >  1:3225573/1‑150 (MQ=255)
                                                                                                                ccATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGc                                      >  1:792356/1‑150 (MQ=255)
                                                                                                                   tctcTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTa                                   >  1:1017755/1‑150 (MQ=255)
                                                                                                                          tAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACg                            >  2:1622985/1‑150 (MQ=255)
                                                                                                                           aaGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGt                           >  1:770334/1‑150 (MQ=255)
                                                                                                                           aaGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGt                           >  1:1717846/1‑150 (MQ=255)
                                                                                                                           aaGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGt                           >  1:1139301/1‑150 (MQ=255)
                                                                                                                               ttCGCCCGCCTAAAGATGGTGAACGATATTTTGCGCTGCTGAAATTTAACGAATTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTa                       <  2:3723813/150‑1 (MQ=255)
                                                                                                                                 cgcccgccGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAAc                     >  1:669976/1‑150 (MQ=255)
                                                                                                                                  gcccgccGAAAGAAGGTGACCGCTATTTTGCGCTGCTTAAAGTTAACGATGTTAACTTCGACAAACCTGAACACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACg                    <  2:830444/150‑1 (MQ=255)
                                                                                                                                    ccgccgAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGt                  >  1:3989009/1‑150 (MQ=255)
                                                                                                                                       ccgAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCt               <  2:3783578/150‑1 (MQ=255)
                                                                                                                                         gAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCtcc             >  1:234858/1‑148 (MQ=255)
                                                                                                                                            agaagGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGa          >  1:3534029/1‑150 (MQ=255)
                                                                                                                                              atggTGACCGCTTTTTGGCGCGGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAg        <  1:148586/148‑1 (MQ=255)
                                                                                                                                              aagGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGACGTTAACTTCGACACACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAg        <  2:3986256/150‑1 (MQ=255)
                                                                                                                                               agGTGATCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTGTGAGAACTTAACCCCGCTGCACGCCAACTCTCGTCTGCGGATGGAACGGGGTACCGGTTCTACTGAAgg       >  2:1349208/1‑149 (MQ=255)
                                                                                                                                                ggTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGAt      <  2:2209420/150‑1 (MQ=255)
                                                                                                                                                  tGATCGATATTTTGCGCTGCTTAATGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGAttt    <  1:4678370/150‑1 (MQ=255)
                                                                                                                                                    aaCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTTaa  <  2:3319151/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTTAA  >  CP009273/3959951‑3960248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑