BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,988,075	T→A	62.7%	V65E (GTG→GAG)	yifL →	putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,988,075	0	T	A	62.7%	317.7 / inf	287	V65E (GTG→GAG)	yifL	putative lipoprotein
Reads supporting (aligned to +/- strand): ref base T (49/58); new base A (89/91); total (138/149)
Fisher's exact test for biased strand distribution p-value = 6.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 291 total aligned reads displayed.

AGCCTGACGGGCTGCGGTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCGCCTGGCTG  >  CP009273/3987927‑3988224

aGCCTGACGGGCTGCGGTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTg                                                                                                                                                      >  1:3514528/1‑150 (MQ=255)

 gCCTGACGGGCTGCGGTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGgaga                                                                                                                                                     >  1:2958777/1‑150 (MQ=255)

 gCCTGACGGGCTGCGGTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGgaga                                                                                                                                                     >  2:3318322/1‑150 (MQ=255)

  ccTGACGGGCTGCGGTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGaa                                                                                                                                                    >  1:95998/1‑150 (MQ=255)

    tGACGGGCTGCGGTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAAtt                                                                                                                                                  >  2:2270116/1‑150 (MQ=255)

    tGACGGGCTGCGGTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAAtt                                                                                                                                                  >  1:4039220/1‑150 (MQ=255)

     gACGGGCTGCGGTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTa                                                                                                                                                 >  1:1743213/1‑150 (MQ=255)

      aCGGGCTGCGGTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTAc                                                                                                                                                <  1:3059026/150‑1 (MQ=255)

       cGGGCTGCGGTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACt                                                                                                                                               >  2:4255630/1‑150 (MQ=255)

        gggCTGCGGTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTa                                                                                                                                              <  1:4765968/150‑1 (MQ=255)

        gggCTGCGGTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTa                                                                                                                                              <  1:2316078/150‑1 (MQ=255)

          gCTGCGGTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTaaa                                                                                                                                            >  2:2481630/1‑150 (MQ=255)

                 tCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGt                                                                                                                                     >  2:1452603/1‑150 (MQ=255)

                   tGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGttt                                                                                                                                   >  1:128942/1‑150 (MQ=255)

                    gAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTc                                                                                                                                  >  2:2096720/1‑150 (MQ=255)

                     aaaGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCt                                                                                                                                 <  1:1050415/150‑1 (MQ=255)

                      aaGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTg                                                                                                                                <  2:895060/150‑1 (MQ=255)

                      aaGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTg                                                                                                                                >  2:1508580/1‑150 (MQ=255)

                      aaGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTg                                                                                                                                <  1:2894915/150‑1 (MQ=255)

                      aaGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTg                                                                                                                                >  1:4368656/1‑150 (MQ=255)

                      aaGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTg                                                                                                                                >  1:3691799/1‑150 (MQ=255)

                         gTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTAc                                                                                                                             >  2:3302925/1‑150 (MQ=255)

                              ctctATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCcgcg                                                                                                                        >  1:2807334/1‑150 (MQ=255)

                                ctATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTGTACCCGCGTg                                                                                                                      >  2:4107511/1‑150 (MQ=255)

                                ctATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTGTACCCGCGTg                                                                                                                      >  2:1650902/1‑150 (MQ=255)

                                 tATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGa                                                                                                                     >  2:325493/1‑150 (MQ=255)

                                    ttCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTg                                                                                                                  >  2:3033932/1‑150 (MQ=255)

                                     tCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTgg                                                                                                                 >  2:4560485/1‑150 (MQ=255)

                                       ccGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAg                                                                                                               <  1:1863736/150‑1 (MQ=255)

                                       ccGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAg                                                                                                               >  2:581493/1‑150 (MQ=255)

                                         gCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTa                                                                                                             <  1:3222328/150‑1 (MQ=255)

                                         gCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTa                                                                                                             <  1:792136/150‑1 (MQ=255)

                                          ccTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTaa                                                                                                            <  1:3409230/150‑1 (MQ=255)

                                            tGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAat                                                                                                          <  1:2120130/150‑1 (MQ=255)

                                               aGATAAAAACGCACCGCCGCCGACCACACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAACACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAatgat                                                                                                       <  2:3696663/150‑1 (MQ=255)

                                               aGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTGTACCCGCGGGATTGGAGTAAatgat                                                                                                       >  2:902809/1‑150 (MQ=255)

                                                  tAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCa                                                                                                    >  2:1110302/1‑150 (MQ=255)

                                                  tAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCa                                                                                                    >  2:2430878/1‑150 (MQ=255)

                                                     aaaCGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGtt                                                                                                 >  2:2224895/1‑150 (MQ=255)

                                                       aCGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTtct                                                                                               >  1:1931738/1‑150 (MQ=255)

                                                         gCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCg                                                                                             <  2:1808351/150‑1 (MQ=255)

                                                         gCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCg                                                                                             >  1:4147719/1‑150 (MQ=255)

                                                           aCCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGaa                                                                                           >  2:3469588/1‑150 (MQ=255)

                                                           aCCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGTAGTAAATGATGCAGTTCTCGaa                                                                                           >  2:1588877/1‑150 (MQ=255)

                                                              gccgccgACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAAt                                                                                        <  1:1502964/150‑1 (MQ=255)

                                                                 gccgACCACACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCa                                                                                     <  1:487142/150‑1 (MQ=255)

                                                                  ccgACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCAt                                                                                    <  2:4688728/150‑1 (MQ=255)

                                                                   cgACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATg                                                                                   <  1:4704015/150‑1 (MQ=255)

                                                                    gACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATgg                                                                                  <  2:2954872/150‑1 (MQ=255)

                                                                     aCCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGc                                                                                 >  1:4214470/1‑150 (MQ=255)

                                                                        aacccGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAACAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCtt                                                                              <  1:68081/147‑1 (MQ=255)

                                                                        aaaCCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGTTGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCtt                                                                              <  2:4522965/150‑1 (MQ=255)

                                                                        aaaCCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCtt                                                                              <  2:4196382/150‑1 (MQ=255)

                                                                        aaaCCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCtt                                                                              <  2:4544624/150‑1 (MQ=255)

                                                                           ccGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGc                                                                           <  1:2524584/150‑1 (MQ=255)

                                                                           ccGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGc                                                                           <  1:1787928/150‑1 (MQ=255)

                                                                            cGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCa                                                                          <  1:1146653/150‑1 (MQ=255)

                                                                              gTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAAc                                                                        >  1:1031155/1‑150 (MQ=255)

                                                                              gTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAAc                                                                        >  1:2138254/1‑150 (MQ=255)

                                                                               tAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACg                                                                       >  1:2390014/1‑150 (MQ=255)

                                                                               tAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACg                                                                       >  2:3898547/1‑150 (MQ=255)

                                                                                agagACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGa                                                                      >  2:4446590/1‑150 (MQ=255)

                                                                                    acgcaaacgcaaTCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGAtttt                                                                  <  2:3078865/150‑1 (MQ=255)

                                                                                    acgcaaacgcaaTCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGAtttt                                                                  <  2:2571084/150‑1 (MQ=255)

                                                                                      gcaaacgcaaTCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTAt                                                                >  2:636032/1‑150 (MQ=255)

                                                                                      gcaaacgcaaTCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTAt                                                                >  1:2599242/1‑150 (MQ=255)

                                                                                      gcaaacgcaaTCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTAt                                                                >  2:1334739/1‑150 (MQ=255)

                                                                                       caaacgcaaTCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATg                                                               >  1:2280602/1‑150 (MQ=255)

                                                                                       caaacgcaaTCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATg                                                               >  2:3529186/1‑150 (MQ=255)

                                                                                        aaacgcaaTCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCCGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATgg                                                              >  2:2935273/1‑150 (MQ=255)

                                                                                          acgcaaTCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGgtc                                                            <  1:1563288/150‑1 (MQ=255)

                                                                                           cgcaaTCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGgtcg                                                           <  2:2086120/150‑1 (MQ=255)

                                                                                                    cGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCgg                                                  >  2:2710405/1‑150 (MQ=255)

                                                                                                    cGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCgg                                                  <  2:1751324/150‑1 (MQ=255)

                                                                                                    cGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCgg                                                  <  2:4203628/150‑1 (MQ=255)

                                                                                                     ggTGCCGGATAACAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGt                                                 <  1:4216044/150‑1 (MQ=255)

                                                                                                      gTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTa                                                <  1:1366768/150‑1 (MQ=255)

                                                                                                         ccGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACg                                             >  1:3099942/1‑150 (MQ=255)

                                                                                                             aTAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGa                                         <  2:670418/150‑1 (MQ=255)

                                                                                                                  aaCGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTc                                    <  1:4255630/150‑1 (MQ=255)

                                                                                                                   aCGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCt                                   >  2:4439252/1‑150 (MQ=255)

                                                                                                                     gATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCttt                                 <  2:4039220/150‑1 (MQ=255)

                                                                                                                      aTCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCtttt                                >  1:2258592/1‑150 (MQ=255)

                                                                                                                          cgcCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTc                            <  2:3514528/150‑1 (MQ=255)

                                                                                                                              aCTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCg                        >  2:4388278/1‑150 (MQ=255)

                                                                                                                                 ggCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAg                     <  2:1230894/150‑1 (MQ=255)

                                                                                                                                 ggCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAg                     <  1:4619491/150‑1 (MQ=255)

                                                                                                                                   cGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCt                   <  1:3856239/150‑1 (MQ=255)

                                                                                                                                     aTGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTTTACCCGCGTGATTGGAGTAAATGATGGAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGa                 <  1:835904/150‑1 (MQ=255)

                                                                                                                                     aTGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGa                 <  1:3267380/150‑1 (MQ=255)

                                                                                                                                      tGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGAt                <  2:3784667/150‑1 (MQ=255)

                                                                                                                                        gTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATtc              <  2:3100371/150‑1 (MQ=255)

                                                                                                                                         tCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATtcg             <  2:2516957/150‑1 (MQ=255)

                                                                                                                                          ccATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTC‑TTTTTTCACCGGAGCTGATtcgtc           <  1:2989237/150‑1 (MQ=255)

                                                                                                                                            aTCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATtcgtcg          <  2:265427/150‑1 (MQ=255)

                                                                                                                                             tCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCGc         >  2:481614/1‑150 (MQ=255)

                                                                                                                                               ccAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCGCCt       <  2:3970081/150‑1 (MQ=255)

                                                                                                                                               ccAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCGCCt       <  2:2390014/150‑1 (MQ=255)

                                                                                                                                                  ggTGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCGCCTGGc    >  2:4592064/1‑150 (MQ=255)

                                                                                                                                                   gTGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCGCCTGGCt   <  1:636032/150‑1 (MQ=255)

AGCCTGACGGGCTGCGGTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCGCCTGGCTG  >  CP009273/3987927‑3988224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`