Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,994,247 A→T 70.1% V57E (GTG→GAG)  yigE ← DUF2233 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,994,2470AT70.1% 431.5 / 254.4 254V57E (GTG→GAG) yigEDUF2233 family protein
Reads supporting (aligned to +/- strand):  ref base A (32/44);  new base T (84/94);  total (116/138)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 256 total aligned reads displayed.

TTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCA  >  CP009273/3994098‑3994396
                                                                                                                                                     |                                                                                                                                                     
 ttCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCAc                                                                                                                                                      <  1:2533879/150‑1 (MQ=255)
 ttCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCAc                                                                                                                                                      >  2:1878224/1‑150 (MQ=255)
  tCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTcc                                                                                                                                                     <  1:2332613/150‑1 (MQ=255)
   cGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTccc                                                                                                                                                    <  2:1826620/150‑1 (MQ=255)
     aTGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGc                                                                                                                                                  >  1:1435212/1‑150 (MQ=255)
       gTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGctc                                                                                                                                                <  2:3425719/150‑1 (MQ=255)
        tACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGctct                                                                                                                                               <  2:4127161/150‑1 (MQ=255)
          cAAACCGAGCGGCGCAGAGCTTTCATCAGAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTAGTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGt                                                                                                                                             <  2:2642581/150‑1 (MQ=255)
           aaaCCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGtt                                                                                                                                            >  2:3435308/1‑150 (MQ=255)
            aaCCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGttt                                                                                                                                           >  2:1532292/1‑150 (MQ=255)
            aaCCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGttt                                                                                                                                           <  1:923123/150‑1 (MQ=255)
            aaCCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGttt                                                                                                                                           <  2:333451/150‑1 (MQ=255)
            aaCCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGttt                                                                                                                                           <  2:968814/150‑1 (MQ=255)
               cGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAg                                                                                                                                        >  2:4726271/1‑150 (MQ=255)
                gAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAgg                                                                                                                                       <  2:4544275/150‑1 (MQ=255)
                gAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAgg                                                                                                                                       <  2:1065832/150‑1 (MQ=255)
                  gcggcgCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGAt                                                                                                                                     <  1:12283/150‑1 (MQ=255)
                    ggcgCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTa                                                                                                                                   <  1:1140681/150‑1 (MQ=255)
                       gcATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACg                                                                                                                                >  2:1694804/1‑150 (MQ=255)
                       gcATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACg                                                                                                                                >  1:3603566/1‑150 (MQ=255)
                       gcATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACg                                                                                                                                >  2:407786/1‑150 (MQ=255)
                         aTAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGt                                                                                                                              >  1:3403337/1‑150 (MQ=255)
                          tAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGta                                                                                                                             <  1:2781445/150‑1 (MQ=255)
                          tAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGta                                                                                                                             <  2:1165631/150‑1 (MQ=255)
                           aGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGtat                                                                                                                            <  2:1750268/150‑1 (MQ=255)
                           aGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAAGATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGtat                                                                                                                            <  1:1009940/150‑1 (MQ=255)
                             cTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATAc                                                                                                                          <  1:905781/150‑1 (MQ=255)
                             cTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATAc                                                                                                                          <  2:2190449/150‑1 (MQ=255)
                                 catcatAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATAGCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCt                                                                                                                      <  1:3420795/150‑1 (MQ=255)
                                   tcatAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGt                                                                                                                    <  1:645327/150‑1 (MQ=255)
                                   tcatAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGt                                                                                                                    <  1:1220429/150‑1 (MQ=255)
                                     atAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTAc                                                                                                                  >  2:2129262/1‑150 (MQ=255)
                                      tAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACg                                                                                                                 <  1:4304757/150‑1 (MQ=255)
                                      tAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACg                                                                                                                 <  2:3239105/150‑1 (MQ=255)
                                       aGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACgg                                                                                                                >  1:1684045/1‑150 (MQ=255)
                                       aGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACgg                                                                                                                >  1:726832/1‑150 (MQ=255)
                                        gATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGt                                                                                                               >  2:4567707/1‑150 (MQ=255)
                                          tGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTACTATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCa                                                                                                             <  2:3542864/150‑1 (MQ=255)
                                          tGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCa                                                                                                             >  2:3353101/1‑150 (MQ=255)
                                          tGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCa                                                                                                             <  2:1261645/150‑1 (MQ=255)
                                           gccgccGTACATCGCCATCTGCACCTGACCCTGACTATGAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAg                                                                                                            <  2:4063684/150‑1 (MQ=255)
                                                 gTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCgg                                                                                                      >  1:710672/1‑150 (MQ=255)
                                                  ttCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGa                                                                                                     >  2:3443329/1‑150 (MQ=255)
                                                   tCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGAt                                                                                                    >  2:32221/1‑150 (MQ=255)
                                                      tCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTg                                                                                                 >  1:3305836/1‑150 (MQ=255)
                                                      tCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTg                                                                                                 <  2:1648460/150‑1 (MQ=255)
                                                          cATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCTTTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGagag                                                                                             >  2:4310333/1‑150 (MQ=255)
                                                          cATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGagag                                                                                             <  2:381469/150‑1 (MQ=255)
                                                            tCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTg                                                                                           >  2:3171965/1‑150 (MQ=255)
                                                              tGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTgcg                                                                                         >  2:90327/1‑150 (MQ=255)
                                                              tGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTgcg                                                                                         >  2:861158/1‑150 (MQ=255)
                                                                cACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCa                                                                                       <  2:656819/150‑1 (MQ=255)
                                                                cACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCa                                                                                       >  2:2276883/1‑150 (MQ=255)
                                                                    tgaccctgacTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAatca                                                                                   >  1:1787931/1‑150 (MQ=255)
                                                                      accctgacTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAatcatc                                                                                 <  2:3513683/150‑1 (MQ=255)
                                                                       ccctgacTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAAtcatca                                                                                >  1:2960601/1‑150 (MQ=255)
                                                                        cctgacTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAg                                                                               >  2:4755456/1‑150 (MQ=255)
                                                                        cctgacTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAg                                                                               >  1:1115663/1‑150 (MQ=255)
                                                                             cTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCa                                                                          >  1:1956868/1‑150 (MQ=255)
                                                                             cTATTAATATCCGCCAGCAGAGCATGTAACGGTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCa                                                                          <  2:4778946/150‑1 (MQ=255)
                                                                              tATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCaa                                                                         >  1:423403/1‑150 (MQ=255)
                                                                                 tAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAg                                                                      <  1:4465780/150‑1 (MQ=255)
                                                                                         gCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACA‑TTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAgg                                                             <  1:1148385/150‑1 (MQ=255)
                                                                                            agcagAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGa                                                           >  2:2804751/1‑150 (MQ=255)
                                                                                                  gCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAg                                                     <  2:3138058/150‑1 (MQ=255)
                                                                                                  gCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAg                                                     <  2:3137681/150‑1 (MQ=255)
                                                                                                    aTGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGt                                                   <  1:3892724/150‑1 (MQ=255)
                                                                                                     tGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTg                                                  <  1:159276/150‑1 (MQ=255)
                                                                                                      gTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGa                                                 <  1:3561184/150‑1 (MQ=255)
                                                                                                       tAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAg                                                <  2:4377046/150‑1 (MQ=255)
                                                                                                         aCGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAggg                                              <  1:3876594/150‑1 (MQ=255)
                                                                                                          cGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGc                                             >  2:242144/1‑150 (MQ=255)
                                                                                                            ttCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCga                                           <  1:1532295/150‑1 (MQ=255)
                                                                                                             tCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCgag                                          >  1:3830820/1‑150 (MQ=255)
                                                                                                              ccccACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCgaga                                         <  1:4783242/150‑1 (MQ=255)
                                                                                                              ccccACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCgaga                                         <  2:3485430/150‑1 (MQ=255)
                                                                                                               cccACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGaa                                        >  1:2858189/1‑150 (MQ=255)
                                                                                                               cccACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGaa                                        <  1:4110760/150‑1 (MQ=255)
                                                                                                                ccACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGaaa                                       >  1:2899608/1‑150 (MQ=255)
                                                                                                                ccACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGaaa                                       <  2:1435212/150‑1 (MQ=255)
                                                                                                                 cACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGaaaa                                      <  1:4619446/150‑1 (MQ=255)
                                                                                                                   cGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAAt                                    >  1:4445730/1‑150 (MQ=255)
                                                                                                                   cGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAAt                                    <  2:1992420/150‑1 (MQ=255)
                                                                                                                   cGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAAt                                    <  2:4443998/150‑1 (MQ=255)
                                                                                                                       tCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGt                                >  2:2862268/1‑150 (MQ=255)
                                                                                                                       tCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGt                                >  2:3608623/1‑150 (MQ=255)
                                                                                                                         gCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGttt                              <  1:4400948/150‑1 (MQ=255)
                                                                                                                         gCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGttt                              <  1:4733449/150‑1 (MQ=255)
                                                                                                                           cagtGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTga                            <  1:3984478/147‑1 (MQ=255)
                                                                                                                           cATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTga                            >  1:2625326/1‑150 (MQ=255)
                                                                                                                            aTTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTgag                           >  1:3103371/1‑150 (MQ=255)
                                                                                                                            aTTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTgag                           >  2:2540703/1‑150 (MQ=255)
                                                                                                                              tGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGAt                         <  1:4562142/150‑1 (MQ=255)
                                                                                                                              tGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGAt                         <  1:2112316/150‑1 (MQ=255)
                                                                                                                                gCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTc                       <  2:2715436/150‑1 (MQ=255)
                                                                                                                                 cTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCa                      >  1:35108/1‑150 (MQ=255)
                                                                                                                                      tGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTg                 >  2:3100237/1‑150 (MQ=255)
                                                                                                                                       gCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGa                <  2:4695154/150‑1 (MQ=255)
                                                                                                                                             aCATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTc          <  1:4166322/150‑1 (MQ=255)
                                                                                                                                              cATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTcc         >  2:3997492/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCA  >  CP009273/3994098‑3994396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑