Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,005,640 A→C 22.7% I179M (ATT→ATG)  metR ← methionine biosynthesis regulon transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,005,6400AC22.7% 655.3 / 260.0 334I179M (ATT→ATG) metRmethionine biosynthesis regulon transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (132/125);  new base C (28/48);  total (160/174)
Fisher's exact test for biased strand distribution p-value = 2.71e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01

Only 100 of 338 total aligned reads displayed.

GCAACCATCTGAATCAACAATAAGGTGTTATCGACGCTTTTCAGTGACGGGCTGACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACTGCGCGGCAGAATATCGGACGTCATTACCAGATCCAGCTCTCCCTGTTGCAAGGCGGGCTGCGG  >  CP009273/4005491‑4005789
                                                                                                                                                     |                                                                                                                                                     
gCAACCATCTGAATCAACAATAAGGTGTTATCGACGCTTTTCAGTGACGGGCTGACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTa                                                                                                                                                       >  1:1026055/1‑150 (MQ=255)
   aCCATCTGAATCAACAATAAGGTGTTATCGACGCTTTTCAGTGACGGGCTGACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAAtt                                                                                                                                                    >  2:3140872/1‑150 (MQ=255)
   aCCATCTGAATCAACAATAAGGTGTTATCGACGCTTTTCAGTGACGGGCTGACGCCTGCCGGCGGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTCAtt                                                                                                                                                    <  2:2574896/150‑1 (MQ=255)
    ccATCTGAATCAACAATAAGGTGTTATCGACGCTTTTCAGTGACGGGCTGACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTc                                                                                                                                                   >  2:1630782/1‑150 (MQ=255)
      aTCTGAATCAACAATAAGGTGTTATCGACGCTTTTCAGTGACGGGCTGACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTcgc                                                                                                                                                 >  1:1402692/1‑150 (MQ=255)
         tGAATCAACAATAAGGTGTTATCGACGCTTTTCAGTGACGGGCTGACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTCATTCGCGtt                                                                                                                                              >  2:1044902/1‑150 (MQ=255)
         tGAATCAACAATAAGGTGTTATCGACGCTTTTCAGTGACGGGCTGACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTCATTCGCGtt                                                                                                                                              >  2:3335168/1‑150 (MQ=255)
          gAATCAACACTAAGGTGTTATCGACGCTTTTCAGTGACGGGCTGACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATCGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGttt                                                                                                                                             <  1:840995/150‑1 (MQ=255)
           aaTCAACAATAAGGTGTTATCGACGCTTTTCAGTGACGGGCTGACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTCATTCGCGtttt                                                                                                                                            >  2:4449609/1‑150 (MQ=255)
            aTCAACAATAAGGTGTTATCGACGCTTTTCAGTGACGGGCTGACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTc                                                                                                                                           <  1:1960599/150‑1 (MQ=255)
               aacaaTAAGGTGTTATCGACGCTTTTCAGTGACGGGCTGACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTcgcc                                                                                                                                        >  2:2918975/1‑150 (MQ=255)
                   aTAAGGTGTTATCGACGCTTTTCAGTGACGGGCTGACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTCATTCGCGTTTTCGCCGCCa                                                                                                                                    <  1:1805775/150‑1 (MQ=255)
                   aTAAGGTGTTATCGACGCTTTTCAGTGACGGGCTGACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTCATTCGCGTTTTCGCCGCCa                                                                                                                                    <  1:1176162/150‑1 (MQ=255)
                     caGGTGTTATCGACGCTTTTCAGTGACGGGCTGACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGt                                                                                                                                  <  2:1861822/149‑1 (MQ=255)
                     aaGGTGTTATCGACGCTTTTCAGTGACGGGCTGACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGt                                                                                                                                  >  2:1354969/1‑150 (MQ=255)
                       ggTGTTATCGACGCTTTTCAGTGACGGGCTGACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTgg                                                                                                                                <  2:4391735/150‑1 (MQ=255)
                        gtgtTATCGACGCTTTTCAGTGACGGGCTGACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGa                                                                                                                               >  1:4783955/1‑150 (MQ=255)
                         tgtTATCGACGCTTTTCAGTGACGGGCTGACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGAt                                                                                                                              >  1:4458733/1‑150 (MQ=255)
                          gtTATCGACGCTTTTCAGTGACGGGCTGACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATg                                                                                                                             <  2:1537386/150‑1 (MQ=255)
                            tATCGACGCTTTTCAGTGACGGGCTGACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTCATTCGCGTTTTCGCCGCCAGTGGATGGt                                                                                                                           >  1:3414/1‑150 (MQ=255)
                            tATCGACGCTTTTCAGTGACGGGCTGACGCCAGCCGGCTGAAGAAAATGCCGCCAGACATCCCGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTCATTCGCGTTTTCGCCGCCAGTGGATGGt                                                                                                                           >  1:1499753/1‑150 (MQ=255)
                             aTCGACGCTTTTCAGTGACGGGCTGACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTc                                                                                                                          >  1:3002306/1‑150 (MQ=255)
                              tCGACGCTTTTCAGTGACGGGCTGACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCa                                                                                                                         <  2:4598881/150‑1 (MQ=255)
                                 aCGCTTTTCAGTGACGGGCTGACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGt                                                                                                                      >  2:3026936/1‑150 (MQ=255)
                                   gCTTTTCAGTGACGGGCTGACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGc                                                                                                                    >  2:2682953/1‑150 (MQ=255)
                                       ttCAGTGACGGGCTGACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAac                                                                                                                >  2:2222595/1‑150 (MQ=255)
                                        tCAGTGACGGGCTGACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAaca                                                                                                               >  1:3260885/1‑150 (MQ=255)
                                             gACGGGCTGACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGa                                                                                                          <  2:2611914/150‑1 (MQ=255)
                                              aCGGGCTGACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGAc                                                                                                         <  2:3625033/150‑1 (MQ=255)
                                              aCGGGCTGACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGAc                                                                                                         <  1:1298087/150‑1 (MQ=255)
                                              aCGGGCTGACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGAc                                                                                                         <  2:1963530/150‑1 (MQ=255)
                                                gggCTGACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGc                                                                                                       >  1:190533/1‑150 (MQ=255)
                                                    tGACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACtt                                                                                                   <  2:563858/150‑1 (MQ=255)
                                                     gACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTc                                                                                                  >  1:232770/1‑150 (MQ=255)
                                                     gACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAAGAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTc                                                                                                  <  1:153898/150‑1 (MQ=255)
                                                       cGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCAt                                                                                                >  1:4063427/1‑150 (MQ=255)
                                                        gCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATa                                                                                               <  1:1630782/150‑1 (MQ=255)
                                                        gCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATa                                                                                               <  1:945815/150‑1 (MQ=255)
                                                        gCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATa                                                                                               >  1:4841577/1‑150 (MQ=255)
                                                         ccTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAg                                                                                              <  2:3778089/150‑1 (MQ=255)
                                                          cTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTCATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGt                                                                                             >  2:1412478/1‑150 (MQ=255)
                                                          cTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGt                                                                                             >  2:4082991/1‑150 (MQ=255)
                                                             ccGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGa                                                                                          <  1:3191264/150‑1 (MQ=255)
                                                                gCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTCATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACa                                                                                       <  1:1044902/150‑1 (MQ=255)
                                                                gCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACa                                                                                       >  2:676410/1‑150 (MQ=255)
                                                                 cTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACAt                                                                                      <  2:4030209/150‑1 (MQ=255)
                                                                 cTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACAt                                                                                      <  1:1646406/150‑1 (MQ=255)
                                                                 cTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACAt                                                                                      <  1:4127512/150‑1 (MQ=255)
                                                                  tGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATc                                                                                     <  1:3560576/150‑1 (MQ=255)
                                                                   gaagaaAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTCATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCg                                                                                    <  1:2709479/150‑1 (MQ=255)
                                                                     agaaAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCTCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGc                                                                                  >  2:1267315/1‑150 (MQ=255)
                                                                     agaaAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGc                                                                                  <  2:1684550/150‑1 (MQ=255)
                                                                       aaaaTGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTCATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGa                                                                                >  1:469831/1‑150 (MQ=255)
                                                                            gccgccAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGaataat                                                                           <  2:4333765/150‑1 (MQ=255)
                                                                             ccgccAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATg                                                                          <  1:4191051/150‑1 (MQ=255)
                                                                             ccgccAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATg                                                                          >  1:3906218/1‑150 (MQ=255)
                                                                               gccAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTCATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCa                                                                        <  1:989296/150‑1 (MQ=255)
                                                                               gccAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCTCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCa                                                                        >  1:2839092/1‑150 (MQ=255)
                                                                               gccAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCa                                                                        <  2:521650/150‑1 (MQ=255)
                                                                                 cAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTCATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAgg                                                                      >  1:2030003/1‑150 (MQ=255)
                                                                                   gACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGcc                                                                    >  1:4208187/1‑150 (MQ=255)
                                                                                      aTCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTCAGTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACt                                                                 <  2:1300409/150‑1 (MQ=255)
                                                                                      aTCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACt                                                                 <  1:613716/150‑1 (MQ=255)
                                                                                       tCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTCATTCACGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACTg                                                                <  2:2874225/150‑1 (MQ=255)
                                                                                        ccAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTCATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACTgc                                                               <  2:3573686/150‑1 (MQ=255)
                                                                                           gTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACTGcgcg                                                            >  1:3962751/1‑150 (MQ=255)
                                                                                             cGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACTGCGCGGc                                                          <  1:3137900/150‑1 (MQ=255)
                                                                                              gACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACTGCGCGGCa                                                         >  2:2114181/1‑150 (MQ=255)
                                                                                               aCTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACTGCGCGGCAg                                                        <  1:4005231/150‑1 (MQ=255)
                                                                                                cTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACTGCGCGGCAGa                                                       <  2:4244929/150‑1 (MQ=255)
                                                                                                       gCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACTGCGCGGCAGAATATCgg                                                >  1:2866362/1‑150 (MQ=255)
                                                                                                         aCCGGATAAATTCATAGCGTCTCGCTGGCGAGATCTTCCGGTGTCATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACTGCGCGGCAGAATATCGGAc                                              <  1:1062922/150‑1 (MQ=255)
                                                                                                              aTAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTCATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACTGCGCGGCAGAATATCGGACGTCAt                                         >  1:3090481/1‑150 (MQ=255)
                                                                                                                aaaTTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACTGCGCGGCAGAATATCGGACGTCATTa                                       >  1:1459197/1‑150 (MQ=255)
                                                                                                                aaaTTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACTGCGCGGCAGAATATCGGACGTCATTa                                       >  2:4749025/1‑150 (MQ=255)
                                                                                                                aaaTTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACTGCGCGGCAGAATATCGGACGTCATTa                                       <  2:3846616/150‑1 (MQ=255)
                                                                                                                 aaTTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACTGCGCGGCAGAATATCGGACGTCATTAc                                      >  2:4520185/1‑150 (MQ=255)
                                                                                                                   ttAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACTGCGCGGCAGAATATCGGACGTCATTACCa                                    <  1:2666587/150‑1 (MQ=255)
                                                                                                                   ttAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACTGCGCGGCAGAATATCGGACGTCATTACCa                                    >  1:2378271/1‑150 (MQ=255)
                                                                                                                            tctcGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACTGCGCGGCAGAATATCGGACGTCATTACCAGATCCAGct                           <  2:810277/150‑1 (MQ=255)
                                                                                                                              tcGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACTGCGCGGCAGAATATCGGACGTCATTACCAGATCCAGctct                         >  1:2270235/1‑150 (MQ=255)
                                                                                                                               cGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACTGCGCGGCAGAATATCGGACGTCATTACCAGATCCAGCtctc                        >  1:1779809/1‑150 (MQ=255)
                                                                                                                                 cTGGCGAGATCTTCCGGTGTCATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACTGCGCGGCAGAATATCGGACGTCATTACCAGATCCAGCTCTccc                      <  2:469831/150‑1 (MQ=255)
                                                                                                                                 cTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACTGCGCGGCAGAATATCGGACGTCATTACCAGATCCAGCTCTccc                      >  1:4870690/1‑150 (MQ=255)
                                                                                                                                     cGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACTGCGCGGCAGAATATCGGACGTCATTACCAGATCCAGCTCTCCCTGtt                  >  2:1722204/1‑150 (MQ=255)
                                                                                                                                        gaTCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACTGCGCGGCAGAATATCGGACGTCATTACCAGATCCAGCTCTCCCTGTTGCa               <  2:2286187/150‑1 (MQ=255)
                                                                                                                                         aTCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACTGCGCGGCAGAATATCGGACGTCATTACCAGATCCAGCTCTCCCTGTTGCac              >  2:4125541/1‑149 (MQ=255)
                                                                                                                                         aTCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACTGCGCGGCAGAATATCGGACGTCATTACCAGATCCAGCTCTCCCTGTTGCaa              <  2:150250/150‑1 (MQ=255)
                                                                                                                                          tCTTCCGGTGTCATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACTGCGCGGCAGAATATCGGACGTCATTACCAGATCCAGCTCTCCCTGTTGCAAg             <  1:4104890/150‑1 (MQ=255)
                                                                                                                                          tCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACTGCGCGGCAGAATATCGGACGTCATTACCAGATCCAGCTCTCCCTGTTGCAAg             <  2:856126/150‑1 (MQ=255)
                                                                                                                                            ttCCGGTGTCATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACTGCGCGGCAGAATATCGGACGTCATTACCAGATCCAGCTCTCCCTGTTGCAAGGc           <  2:209978/150‑1 (MQ=255)
                                                                                                                                             tCCGGTGTCATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACTGCGCGGCAGAATATCGGACGTCATTACCAGATCCAGCTCTCCCTGTTGCAAGGCg          <  1:281674/150‑1 (MQ=255)
                                                                                                                                             tCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACTGCGCGGCAGAATATCGGACGTCATTACCAGATCCAGCTCTCCCTGTTGCAAGGCg          >  1:600683/1‑150 (MQ=255)
                                                                                                                                             tCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACTGCGCGGCAGAATATCGGACGTCATTACCAGATCCAGCTCTCCCTGTTGCAAGGCg          <  1:4598574/150‑1 (MQ=255)
                                                                                                                                              ccGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACTGCGCGGCAGAATATCGGACGTCATTACCAGATCCAGCTCTCCCTGTTGCAAGGCgg         <  1:3387706/150‑1 (MQ=255)
                                                                                                                                                ggTGTCATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACTGCGCGGCAGAATATCGGACGTCATTACCAGATCCAGCTCTCCCTGTTGCAAGGCGGGc       >  2:115629/1‑150 (MQ=255)
                                                                                                                                                 gtgtAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACTGCGCGGCAGAATATCGGACGTCATTACCAGATCCAGCTCTCCCTGTTGCAAGGCGGGCt      <  2:2850240/150‑1 (MQ=255)
                                                                                                                                                 gtgtAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACTGCGCGGCAGAATATCGGACGTCATTACCAGATCCAGCTCTCCCTGTTGCAAGGCGGGCt      <  1:3778338/150‑1 (MQ=255)
                                                                                                                                                  tgtAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACTGCGCGGCAGAATATCGGACGTCATTACCAGATCCAGCTCTCCCTGTTGCAAGGCGGGCTg     >  1:302929/1‑150 (MQ=255)
                                                                                                                                                   gtAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACTGCGCGGCAGAATATCGGACGTCATTACCAGATCCAGCTCTCCCTGTTGCAAGGCGGGCTGc    >  2:4308920/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCAACCATCTGAATCAACAATAAGGTGTTATCGACGCTTTTCAGTGACGGGCTGACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGACTACGCTGCACCGGATAAATTAATAGCGTCTCGCTGGCGAGATCTTCCGGTGTAATTCGCGTTTTCGCCGCCAGTGGATGGTCAGGTGCTAACACCAGACGCACTTCATAGTCGAACATCGGCGAATAATGCAGGCCACTGCGCGGCAGAATATCGGACGTCATTACCAGATCCAGCTCTCCCTGTTGCAAGGCGGGCTGCGG  >  CP009273/4005491‑4005789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑