BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	308,879	G→A	13.6%	Y25Y (TAC→TAT)	ykgR ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	308,879	0	G	A	13.6%	357.8 / 40.3	125	Y25Y (TAC→TAT)	ykgR	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base G (58/50); new base A (11/6); total (69/56)
Fisher's exact test for biased strand distribution p-value = 4.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

Only 100 of 125 total aligned reads displayed.

GATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAAT  >  CP009273/308739‑309027

gattgattACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACa                                                                                                                                              <  2:2148836/150‑1 (MQ=255)

  ttgattACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAAt                                                                                                                                            <  2:759373/150‑1 (MQ=255)

  ttgagtACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAAt                                                                                                                                            <  1:2464050/150‑1 (MQ=255)

    gattACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTg                                                                                                                                          >  1:3773623/1‑150 (MQ=255)

     attACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGc                                                                                                                                         <  1:2198893/150‑1 (MQ=255)

      ttACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCg                                                                                                                                        >  2:2720416/1‑150 (MQ=255)

       tACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGa                                                                                                                                       >  1:1688437/1‑150 (MQ=255)

         ccTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACa                                                                                                                                     >  2:4176676/1‑150 (MQ=255)

           tGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACaaa                                                                                                                                   >  2:4870242/1‑150 (MQ=255)

             aCAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACaaaaa                                                                                                                                 >  2:4530650/1‑150 (MQ=255)

                gCGTGTATTCCTCTCCCACGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAaacaa                                                                                                                              <  1:709307/150‑1 (MQ=255)

                    gtATTCCTCTCCCACTGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGat                                                                                                                          <  2:3292122/150‑1 (MQ=255)

                      aTTCCTCTCCCACGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCATATTCTACAATTGCGACAAAAACAACGatat                                                                                                                        <  1:1587527/150‑1 (MQ=255)

                         ccTCTCCCACGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTaa                                                                                                                     >  1:3850131/1‑150 (MQ=255)

                         ccTCTCCCACGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTaa                                                                                                                     >  1:3028943/1‑150 (MQ=255)

                           tctcCCACGGATATAATAATTACTTACAAAAAAGTGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCTTTATTAATATAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATc                                                                                                                   <  1:3663662/150‑1 (MQ=255)

                           tctcCCACGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCATATTCTACAATTGCGACAAAAACAACGATATTAATc                                                                                                                   >  1:3048566/1‑150 (MQ=255)

                                cACGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGttt                                                                                                              <  1:93686/150‑1 (MQ=255)

                                cACGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGttt                                                                                                              <  1:4178515/150‑1 (MQ=255)

                                   ggATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATg                                                                                                           <  1:4391523/150‑1 (MQ=255)

                                     atatAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGAc                                                                                                         <  1:1951737/150‑1 (MQ=255)

                                     atatAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGAc                                                                                                         <  1:4605116/150‑1 (MQ=255)

                                       ataataATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTg                                                                                                       >  2:4256923/1‑150 (MQ=255)

                                       ataataATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATAAGTTTATGACTg                                                                                                       >  2:4256916/1‑150 (MQ=255)

                                        taataaTTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑a                                                                                                     <  1:1125874/150‑1 (MQ=255)

                                         aataatTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑At                                                                                                    <  1:1107183/150‑1 (MQ=255)

                                          ataatTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑Att                                                                                                   >  1:3290525/1‑150 (MQ=255)

                                          ataatTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑Att                                                                                                   >  1:498518/1‑150 (MQ=255)

                                           taatTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑Attt                                                                                                  <  1:1684998/150‑1 (MQ=255)

                                           taatTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑Attt                                                                                                  <  1:1683812/150‑1 (MQ=255)

                                            aatTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTg                                                                                                 >  1:3179932/1‑150 (MQ=255)

                                            aatTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTg                                                                                                 <  2:4676756/150‑1 (MQ=255)

                                                 cttacAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTa                                                                                            >  2:1188663/1‑150 (MQ=255)

                                                    acAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACtt                                                                                         >  1:1244370/1‑150 (MQ=255)

                                                     cAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACttt                                                                                        <  1:774478/150‑1 (MQ=255)

                                                      aaaaaaGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTa                                                                                       >  1:813730/1‑150 (MQ=255)

                                                       aaaaaGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTAt                                                                                      >  2:3212968/1‑150 (MQ=255)

                                                       aaaaaGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTAt                                                                                      >  2:779311/1‑150 (MQ=255)

                                                       aaaaaGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTAt                                                                                      >  2:1206855/1‑150 (MQ=255)

                                                        aaaaGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTAtt                                                                                     >  1:4614252/1‑150 (MQ=255)

                                                        aaaaGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTAtt                                                                                     >  1:4614481/1‑150 (MQ=255)

                                                            gggAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCTTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTctct                                                                                 <  2:3267695/150‑1 (MQ=255)

                                                              gagaGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCttt                                                                               <  2:1986920/150‑1 (MQ=255)

                                                                gagGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCATATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCa                                                                             <  1:3117900/150‑1 (MQ=255)

                                                                gagGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCATATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCa                                                                             <  1:3117935/150‑1 (MQ=255)

                                                                 agGATGCATCTTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCAt                                                                            <  2:4768883/150‑1 (MQ=255)

                                                                 agGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCAt                                                                            <  2:4769211/150‑1 (MQ=255)

                                                                  ggATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCAtt                                                                           >  1:3624562/1‑150 (MQ=255)

                                                                   gATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTg                                                                          <  2:1914277/150‑1 (MQ=255)

                                                                     tGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGt                                                                        >  2:4078181/1‑150 (MQ=255)

                                                                      gCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCATATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTa                                                                       >  2:3319175/1‑150 (MQ=255)

                                                                        atatTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACt                                                                     <  1:2347748/150‑1 (MQ=255)

                                                                            tttAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCt                                                                 <  1:638977/150‑1 (MQ=255)

                                                                            tttAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCt                                                                 >  1:2240408/1‑150 (MQ=255)

                                                                             ttAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTc                                                                >  1:554845/1‑150 (MQ=255)

                                                                             ttAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTc                                                                >  1:2520198/1‑150 (MQ=255)

                                                                                aaTATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCt                                                             >  2:2854764/1‑150 (MQ=255)

                                                                                   atCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTa                                                          >  2:4368952/1‑150 (MQ=255)

                                                                                    tCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTaa                                                         >  2:1222026/1‑150 (MQ=255)

                                                                                      aCTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTaaaa                                                       <  1:3877255/150‑1 (MQ=255)

                                                                                      aCTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTaaaa                                                       <  1:3877248/150‑1 (MQ=255)

                                                                                      aCTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCATATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTaaaa                                                       >  1:4636149/1‑150 (MQ=255)

                                                                                          aaGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAaga                                                   >  2:2521767/1‑150 (MQ=255)

                                                                                           aGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAagag                                                  >  1:1186494/1‑150 (MQ=255)

                                                                                           aGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAagag                                                  <  2:682356/150‑1 (MQ=255)

                                                                                             tGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCATATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTg                                                >  1:4487556/1‑150 (MQ=255)

                                                                                             tGAACAGTTCATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTg                                                <  2:486726/150‑1 (MQ=255)

                                                                                               aaCAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCa                                              >  2:1245976/1‑150 (MQ=255)

                                                                                                aCAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCAc                                             <  2:4748379/150‑1 (MQ=255)

                                                                                                    tttATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCg                                         >  1:4148810/1‑150 (MQ=255)

                                                                                                    tttATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCg                                         >  2:340923/1‑150 (MQ=255)

                                                                                                    tttATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCg                                         >  2:367614/1‑150 (MQ=255)

                                                                                                    tttATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCg                                         <  2:2659811/150‑1 (MQ=255)

                                                                                                    tttATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATAGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCg                                         >  2:341184/1‑150 (MQ=255)

                                                                                                     ttATTTACGTTATTAATTGTAATTGTTCAATAAATTTGCGTTTTAATAAATTGCGAAAAAAAAAACGATTTTAATAATTTTTTTAATGTTTTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCg                                         <  2:888333/150‑1 (MQ=255)

                                                                                                       aTTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCGTaa                                      >  2:3181545/1‑150 (MQ=255)

                                                                                                         ttCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCGTAAGt                                    >  2:59037/1‑150 (MQ=255)

                                                                                                         ttCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCGTAAGt                                    >  2:58951/1‑150 (MQ=255)

                                                                                                             gTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCGTAAGTGccc                                >  2:3206740/1‑150 (MQ=255)

                                                                                                             gTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCGTAAGTGccc                                >  2:1345454/1‑150 (MQ=255)

                                                                                                                    aTAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCGTAAGTGCCCTtatata                         <  1:3579721/150‑1 (MQ=255)

                                                                                                                    aTAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCGTAAGTGCCCTtatata                         <  1:3579679/150‑1 (MQ=255)

                                                                                                                     tAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCGTAAGTGCCCTTATataa                        >  2:3855042/1‑150 (MQ=255)

                                                                                                                       gAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCGTAAGTGCCCTTATataaat                      >  1:2116974/1‑150 (MQ=255)

                                                                                                                         aaTGGAGAAATAAATAGGCATATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCGTAAGTGCCCTTATataaataa                    >  1:1588393/1‑150 (MQ=255)

                                                                                                                         aaTGGAGAAATAAATAGGCATATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCGTAAGTGCCCTTATataaataa                    >  1:1587928/1‑150 (MQ=255)

                                                                                                                          aTGGAGAAATAAATAGGCATATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCGTAAGTGCCCTTATATAAATAAc                   >  2:1161665/1‑150 (MQ=255)

                                                                                                                           tGGAGAAATAAATAGTCGTATTCTACAATTGCTACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCGTAAGTGCCCTTATATAAATAACg                  <  1:4330118/150‑1 (MQ=255)

                                                                                                                           tGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCGTAAGTGCCCTTATATAAATAACg                  <  2:1149504/150‑1 (MQ=255)

                                                                                                                           tGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCGTAAGTGCCCTTATATAAATAACg                  <  2:1148914/150‑1 (MQ=255)

                                                                                                                            ggAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCGTAAGTGCCCTTATATAAATAACGa                 >  1:137590/1‑150 (MQ=255)

                                                                                                                               gaAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCGTAAGTGCCCTTATATAAATAACGAGtt              >  1:2475229/1‑150 (MQ=255)

                                                                                                                               gaAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCGTAAGTGCCCTTATATAAATAACGAGtt              >  1:44990/1‑150 (MQ=255)

                                                                                                                                aaataaatAGGCATATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCGTAAGTGCCCTTATATAAATAACGAGttt             >  1:4822899/1‑150 (MQ=255)

                                                                                                                                aaataaatAGGCATATTCTACAATTGCGACAAAAACAACGATATTAAGCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCGTAAGTGCCCTTATATAAATAACGAGttt             >  2:2113707/1‑150 (MQ=255)

                                                                                                                                      ataGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCaa       <  2:3083100/150‑1 (MQ=255)

                                                                                                                                      ataGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCaa       <  2:3083162/150‑1 (MQ=255)

                                                                                                                                       taGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAAc      >  2:3106311/1‑150 (MQ=255)

                                                                                                                                         ggCATATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCGTAAGTGCCCTTATATAAATAAAGAGTTTGGTCATCCa    >  2:4069448/1‑150 (MQ=255)

                                                                                                                                           cGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAAt  <  1:2413944/150‑1 (MQ=255)

GATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTG‑ATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAAT  >  CP009273/308739‑309027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`