Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,148,380 C→T 25.8% intergenic (‑58/‑38) oxyS ← / → oxyR sRNA antisense regulator activates genes that detoxify oxidative damage, Hfq‑dependent/oxidative and nitrosative stress transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,148,3800CT25.8% 509.9 / 197.1 264intergenic (‑58/‑38)oxyS/oxyRsRNA antisense regulator activates genes that detoxify oxidative damage, Hfq‑dependent/oxidative and nitrosative stress transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (124/71);  new base T (39/29);  total (164/100)
Fisher's exact test for biased strand distribution p-value = 3.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 267 total aligned reads displayed.

CAAAAGTTCACGTTGGCTTTAGTTATTCGAGTTGAGAAACTCTCGAAACGGGCAGTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTA  >  CP009273/4148232‑4148529
                                                                                                                                                    |                                                                                                                                                     
cAAAAGTTCACGTTGGCTTTAGTTATTCGAGTTGAGAAACTCTCGAAACGGGCAGTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCt                                                                                                                                                      >  2:2074682/1‑150 (MQ=255)
  aaaGTTCACGTTGGCTTTAGTTATTCGAGTTGAGAAACTCTCGAAACGGGCAGTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTAt                                                                                                                                                    >  2:4048841/1‑150 (MQ=255)
    aGTTCACGTTGGCTTTAGTTATTCGAGTTGAGAAACTCTCGAAACGGGCAGTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCg                                                                                                                                                  >  1:156141/1‑150 (MQ=255)
    aGTTCACGTTGGCTTTAGTTATTCGAGTTGAGAAACTCTCGAAACGGGCAGTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCg                                                                                                                                                  <  2:10628/150‑1 (MQ=255)
      ttCACGTTGGCTTTAGTTATTCGAGTTGAGAAACTCTCGAAACGGGCAGTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACTTATCGcc                                                                                                                                                <  1:2226438/150‑1 (MQ=255)
      ttCACGTTGGCTTTAGTTATTCGAGTTGAGAAACTCTCGAAACGGGCAGTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGcc                                                                                                                                                >  2:1414058/1‑150 (MQ=255)
        cACGTTGGCTTTAGTTATTCGAGTTGAGAAACTCTCGAAACGGGCAGTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACTTATCGCCAt                                                                                                                                              <  2:2977162/150‑1 (MQ=255)
        cACGTTGGCTTTAGTTATTCGAGTTGAGAAACTCTCGAAACGGGCAGTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCAt                                                                                                                                              >  1:3960492/1‑150 (MQ=255)
         aCGTTGGCTTTAGTTATTCGAGTTGAGAAACTCTCGAAACGGGCAGTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATg                                                                                                                                             <  1:1748580/150‑1 (MQ=255)
          cGTTGGCTTTAGTTATTCGAGTTGAGAAACTCTCGAAACGGGCAGTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGa                                                                                                                                            >  2:1745783/1‑150 (MQ=255)
             tGGCTTTAGTTATTCGAGTTGAGAAACTCTCGAAACGGGCAGTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACt                                                                                                                                         >  2:396081/1‑150 (MQ=255)
             tGGCTTTAGTTATTCGAGTTGAGAAACTCTCGAAACGGGCAGTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACt                                                                                                                                         >  2:1499508/1‑150 (MQ=255)
             tGGCTTTAGTTATTCGAGTTGAGAAACTCTCGAAACGGGCAGTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACt                                                                                                                                         >  1:3733330/1‑150 (MQ=255)
               gCTTTAGTTATTCGAGTTGAGAAACTCTCGAAACGGGCAGTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTAt                                                                                                                                       >  2:967301/1‑150 (MQ=255)
                cTTTAGTTATTCGAGTTGAGAAACTCTCGAAACGGGCAGTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACTTATCGCCATGAACTATc                                                                                                                                      <  1:1362374/150‑1 (MQ=255)
                 tttAGTTATTCGAGTTGAGAAACTCTCGAAACGGGCAGTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACTTATCGCCATGAACTATCg                                                                                                                                     >  2:1180053/1‑150 (MQ=255)
                       tATTCGAGTTGAGAAACTCTCGAAACGGGCAGTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGa                                                                                                                               >  1:2717675/1‑150 (MQ=255)
                        aTTCGAGTTGAGAAACTCTCGAAACGGGCAGTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACTTATCGCCATGAACTATCGTGGCAAt                                                                                                                              <  1:4808976/150‑1 (MQ=255)
                         ttCGAGTTGAGAAACTCTCGAAACGGGCAGTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATg                                                                                                                             >  1:3501932/1‑150 (MQ=255)
                           cGAGTTGAGAAACTCTCGAAACGGGCAGTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACTTATCGCCATGAACTATCGTGGCAATgga                                                                                                                           <  2:587449/150‑1 (MQ=255)
                           cGAGTTGAGAAACTCTCGAAACGGGCAGTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACTTATCGCCATGAACTATCGTGGCAATgga                                                                                                                           <  2:587442/150‑1 (MQ=255)
                           cGAGTTGAGAAACTCTCGAAACGGGCAGTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATgga                                                                                                                           >  1:2516102/1‑150 (MQ=255)
                             aGTTGAGAAACTCTCGAAACGGGCAGTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATggagg                                                                                                                         >  1:3218795/1‑150 (MQ=255)
                               ttGAGAAACTCTCGAAACGGGCAGTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAggat                                                                                                                       <  1:3582102/150‑1 (MQ=255)
                               ttGAGAAACTCTCGAAACGGGCAGTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAggat                                                                                                                       <  2:2855957/150‑1 (MQ=255)
                                 gagaAACTCTCGAAACGGGCAGTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCTTGGCGATGTAggatgg                                                                                                                     >  2:4158313/1‑150 (MQ=255)
                                  agaAACTCTCGAAACGGGCAGTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAggatgga                                                                                                                    <  1:4674901/150‑1 (MQ=255)
                                    aaaCTCTCGAAACGGGCAGTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACTTATCGCCATGAACTATCGTGGCAATGGAGGATGGATa                                                                                                                  >  2:3810788/1‑150 (MQ=255)
                                      aCTCTCGAAACGGGCAGTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACTTATCGCCATGAACTATCGTGGCAATGGAGGATGGATAAt                                                                                                                >  1:3906107/1‑150 (MQ=255)
                                      aCTCTCGAAACGGGCAGTGAATTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAAt                                                                                                                >  2:430157/1‑150 (MQ=255)
                                       ctctCGAAACGGGCAGTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATggagga                                                                                                                        >  1:3717283/1‑141 (MQ=255)
                                          tcGAAACGGGCAGTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACTTATCGCCATGAACTATCGTGGCAATGGAGGATGGATAATGAat                                                                                                            >  2:4507415/1‑150 (MQ=255)
                                           cGAAACGGGCAGTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCAGGAACTATCGTGGCAATGGAGGATGGATAATGAata                                                                                                           >  1:512144/1‑150 (MQ=255)
                                            gAAACGGGCAGTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACTTATCGCCATGAACTATCGTGGCAATGGAGGATGGATAATGAatat                                                                                                          >  1:4269912/1‑150 (MQ=255)
                                                cGGGCAGTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACTTATCGCCATGAACTATCGTGGCAATGGAGGATGGATAATGAATATTCGt                                                                                                      >  1:265674/1‑150 (MQ=255)
                                                  ggCAGTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACTTATCGCCATGAACTATCGTGGCAATGGAGGATGGATAATGAATATTCGTGa                                                                                                    >  2:2447786/1‑150 (MQ=255)
                                                  ggCAGTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGa                                                                                                    >  2:2774252/1‑150 (MQ=255)
                                                     aGTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCt                                                                                                 >  2:1750733/1‑150 (MQ=255)
                                                     aGTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCt                                                                                                 <  1:2940898/150‑1 (MQ=255)
                                                      gTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCtt                                                                                                <  1:640519/150‑1 (MQ=255)
                                                      gTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCtt                                                                                                >  1:1606012/1‑150 (MQ=255)
                                                          cTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACTTATCGCCATGAACTATCGTGGCAATGGAGGATGGATAATGAATATTCGTGATCTTGAGt                                                                                            <  1:9241/150‑1 (MQ=255)
                                                          cTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGt                                                                                            <  2:152832/150‑1 (MQ=255)
                                                          cTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGt                                                                                            >  1:1983626/1‑150 (MQ=255)
                                                               aGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCtg                                                                                       <  1:2449175/150‑1 (MQ=255)
                                                               aGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCtg                                                                                       <  1:2153163/150‑1 (MQ=255)
                                                                 ggTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACTTATCGCCATGAACTATCGTGGCAATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCtggt                                                                                     <  1:1931944/150‑1 (MQ=255)
                                                                    tAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGc                                                                                  >  2:3474349/1‑150 (MQ=255)
                                                                     aaaaGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCa                                                                                 >  1:1870124/1‑150 (MQ=255)
                                                                      aaaGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACTTATCGCCATGAACTATCGTGGCAATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCAt                                                                                >  2:3923051/1‑150 (MQ=255)
                                                                        agagGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACTTATCGCCATGAACTATCGTGGCAATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTg                                                                              <  1:2517566/150‑1 (MQ=255)
                                                                           ggTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCt                                                                           <  2:2478598/150‑1 (MQ=255)
                                                                            gTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTg                                                                          <  2:789916/150‑1 (MQ=255)
                                                                              gCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACTTATCGCCATGAACTATCGTGGCAATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGaa                                                                        <  1:4391089/150‑1 (MQ=255)
                                                                              gCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGaa                                                                        >  1:999960/1‑150 (MQ=255)
                                                                              gCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGaa                                                                        >  1:950009/1‑150 (MQ=255)
                                                                                    ccGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGc                                                                  >  1:2217084/1‑150 (MQ=255)
                                                                                     cGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGcc                                                                 >  2:907348/1‑150 (MQ=255)
                                                                                           tgtgAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACTTATCGCCATGAACTATCGTGGCAATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTc                                                           <  1:1770331/150‑1 (MQ=255)
                                                                                                  aaTTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGc                                                    >  2:4474900/1‑150 (MQ=255)
                                                                                                   aTTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACTTATCGCCATGAACTATCGTGGCAATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCg                                                   >  2:1619239/1‑150 (MQ=255)
                                                                                                     tATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGc                                                 >  2:1315871/1‑150 (MQ=255)
                                                                                                       tcagtcagAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAg                                               >  2:3682081/1‑150 (MQ=255)
                                                                                                           tcagAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTc                                           >  2:2322506/1‑150 (MQ=255)
                                                                                                            cagAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACTTATCGCCATGAACTATCGTGGCAATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCA‑TTTCGGCGTGCGGCAGATTCCt                                         >  2:4342808/1‑150 (MQ=255)
                                                                                                             agAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCt                                         >  2:4428236/1‑150 (MQ=255)
                                                                                                               aaTGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGc                                       <  2:2163140/150‑1 (MQ=255)
                                                                                                                  gCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCAc                                    >  2:2371244/1‑150 (MQ=255)
                                                                                                                   cTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACg                                   <  2:2926194/150‑1 (MQ=255)
                                                                                                                      gATAGGGATAATCGTTCATTGCTATTCTACTTATCGCCATGAACTATCGTGGCAATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTa                                >  2:1195774/1‑150 (MQ=255)
                                                                                                                      gATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTa                                >  1:3366947/1‑150 (MQ=255)
                                                                                                                       aTAGGTATAATCGTAAATTGCTATTCTACATATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATAATCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGAAGATTCCTGACACGTTag                               >  2:2302514/1‑150 (MQ=255)
                                                                                                                       aTAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTag                               >  2:336150/1‑150 (MQ=255)
                                                                                                                          gggATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTagcca                            <  1:4048841/150‑1 (MQ=255)
                                                                                                                           ggATAATCGTTCATTGCTATTCTACTTATCGCCATGAACTATCGTGGCAATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTagccag                           <  2:2097951/150‑1 (MQ=255)
                                                                                                                            gATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTagccagc                          >  2:2972389/1‑150 (MQ=255)
                                                                                                                             aTACTCGTTCAGTGCTATTCTACCTATCGCCAGGAACTATCGTGGCGATGGAGGAGGGATAATGAATATTCGTGATCTTGAGGACCGGGTGGCATTGGCTGAACACCGCCATGTTCGGCGTGCGGCAGATTCCTGCCACGTTagccagcc                         <  1:2774741/150‑1 (MQ=255)
                                                                                                                             aTAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTagccagcc                         >  1:3278774/1‑150 (MQ=255)
                                                                                                                              tcaTCGTTCATTGCTATTCTACCTATCGCCAGGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCg                        >  1:1546531/3‑150 (MQ=255)
                                                                                                                              tAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCg                        <  1:2614173/150‑1 (MQ=255)
                                                                                                                               aaTCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGa                       >  2:3566717/1‑150 (MQ=255)
                                                                                                                                  cGTTCATTGCTATTCTACTTATCGCCATGAACTATCGTGGCAATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGc                    <  1:4507280/150‑1 (MQ=255)
                                                                                                                                     tCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTa                 >  1:4565025/1‑150 (MQ=255)
                                                                                                                                      cATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAg                >  2:375107/1‑150 (MQ=255)
                                                                                                                                       aTTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGc               <  2:3076479/150‑1 (MQ=255)
                                                                                                                                        ttGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTagc                              <  1:2427794/134‑1 (MQ=255)
                                                                                                                                        ttGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCg              >  2:1954829/1‑150 (MQ=255)
                                                                                                                                          gCTATTCTACTTATCGCCATGAACTATCGTGGCAATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCggg            >  2:7331/1‑150 (MQ=255)
                                                                                                                                          gCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCggg            <  2:754278/150‑1 (MQ=255)
                                                                                                                                           cTATTCTACTTATCGCCATGAACTATCGTGGCAATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGc           >  1:757663/1‑150 (MQ=255)
                                                                                                                                           cTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGc           >  2:3026138/1‑150 (MQ=255)
                                                                                                                                             aTTCTACTTATCGCCATGAACTATCGTGGCAATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCaa         >  1:1174202/1‑150 (MQ=255)
                                                                                                                                             aTTCTACTTATCGCCATGAACTATCGTGGCAATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCAATTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCaa         >  1:1173585/1‑150 (MQ=255)
                                                                                                                                             aTTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCaa         >  2:2665663/1‑150 (MQ=255)
                                                                                                                                             aTTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCaa         >  2:4846098/1‑150 (MQ=255)
                                                                                                                                                cTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAAtt      >  2:2158901/1‑150 (MQ=255)
                                                                                                                                                 tACTTATCGCCATGAACTATCGTGGCAATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTc     <  1:4011606/150‑1 (MQ=255)
                                                                                                                                                 tACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTc     >  1:4236840/1‑150 (MQ=255)
                                                                                                                                                 tACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTc     >  1:4526561/1‑150 (MQ=255)
                                                                                                                                                   ccTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGt   >  1:2310543/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CAAAAGTTCACGTTGGCTTTAGTTATTCGAGTTGAGAAACTCTCGAAACGGGCAGTGACTTCAAGGGTTAAAAGAGGTGCCGCTCCGTTTCTGTGAGCAATTATCAGTCAGAATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTA  >  CP009273/4148232‑4148529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑