Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,273,868 C→A 20.7% M321I (ATG→ATT)  actP ← acetate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,273,8680CA20.7% 774.6 / 153.3 281M321I (ATG→ATT) actPacetate transporter
Reads supporting (aligned to +/- strand):  ref base C (124/98);  new base A (29/29);  total (153/128)
Fisher's exact test for biased strand distribution p-value = 4.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 284 total aligned reads displayed.

ACCGCGAGGATAGTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTACCAAACAT  >  CP009273/4273719‑4274017
                                                                                                                                                     |                                                                                                                                                     
accGCGAGGATAGTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGc                                                                                                                                                       >  1:2045646/1‑150 (MQ=255)
     gAGGATAGTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGAt                                                                                                                                                  >  2:3808174/1‑150 (MQ=255)
     gAGGATAGTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGAt                                                                                                                                                  >  1:4833426/1‑150 (MQ=255)
      aGGATAGTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATc                                                                                                                                                 >  1:2870754/1‑150 (MQ=255)
      aGGATAGTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATc                                                                                                                                                 >  2:700819/1‑150 (MQ=255)
      aGGATAGTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGAATGATc                                                                                                                                                 <  1:4440155/150‑1 (MQ=255)
        gATAGTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATcgc                                                                                                                                               <  1:2858175/150‑1 (MQ=255)
             tGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGa                                                                                                                                          >  2:1437693/1‑150 (MQ=255)
               gCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGAATGATCGCGCCGAAg                                                                                                                                        >  1:1212442/1‑150 (MQ=255)
                 gAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGcc                                                                                                                                      >  1:4706770/1‑150 (MQ=255)
                 gAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGcc                                                                                                                                      <  1:73145/150‑1 (MQ=255)
                 gAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGcc                                                                                                                                      >  1:3730547/1‑150 (MQ=255)
                 gAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGcc                                                                                                                                      <  1:2931088/150‑1 (MQ=255)
                 gAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGcc                                                                                                                                      <  1:1364356/150‑1 (MQ=255)
                 gAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGcc                                                                                                                                      <  1:2504092/150‑1 (MQ=255)
                 gAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGAATGATCGCGCCGAAGcc                                                                                                                                      >  2:4270213/1‑150 (MQ=255)
                  aaaGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCg                                                                                                                                     >  1:4320635/1‑150 (MQ=255)
                  aaaGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCg                                                                                                                                     >  1:1995504/1‑150 (MQ=255)
                  aaaGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCg                                                                                                                                     <  2:570750/150‑1 (MQ=255)
                  aaaGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGAGTCGCACCAACCAGCATGATCGCGCCGAAGCCg                                                                                                                                     <  1:4599824/150‑1 (MQ=255)
                    aGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGAGTCGCACCAACCAGCATGATCGCGCCGAAGCCGat                                                                                                                                   <  1:4097605/150‑1 (MQ=255)
                         ccGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGAtaataa                                                                                                                              <  2:4602431/150‑1 (MQ=255)
                          cGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATaaa                                                                                                                             <  2:708454/150‑1 (MQ=255)
                           gCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAg                                                                                                                            >  1:2524566/1‑150 (MQ=255)
                              gAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTc                                                                                                                         >  1:2197570/1‑150 (MQ=255)
                              gAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTc                                                                                                                         >  1:1521586/1‑150 (MQ=255)
                               aaaTAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCa                                                                                                                        >  2:1170693/1‑150 (MQ=255)
                                aaTAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAg                                                                                                                       <  2:169106/150‑1 (MQ=255)
                                    aaaCCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAata                                                                                                                   <  2:564272/150‑1 (MQ=255)
                                     aaCCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAatat                                                                                                                  >  1:3404607/1‑150 (MQ=255)
                                     aaCCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAatat                                                                                                                  <  2:3211775/150‑1 (MQ=255)
                                       ccGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGAATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAg                                                                                                                <  1:3130083/150‑1 (MQ=255)
                                         gAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGAATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGaa                                                                                                              <  1:329840/150‑1 (MQ=255)
                                         gAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGAATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGaa                                                                                                              >  1:3703182/1‑150 (MQ=255)
                                          aGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAg                                                                                                             <  2:3728770/150‑1 (MQ=255)
                                           ggAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGt                                                                                                            <  2:3987320/150‑1 (MQ=255)
                                             aaCAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGACGCCGATAATAAAGGTCAGAATATAGAAGTAg                                                                                                          >  1:1711302/1‑150 (MQ=255)
                                              aCAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGc                                                                                                         <  2:1636769/150‑1 (MQ=255)
                                              aCAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGAATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGc                                                                                                         <  2:2427435/150‑1 (MQ=255)
                                                 ggTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCa                                                                                                      >  2:4353597/1‑150 (MQ=255)
                                                  gTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCAt                                                                                                     >  2:3306245/1‑150 (MQ=255)
                                                   ttGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGAATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATa                                                                                                    >  2:2537178/1‑150 (MQ=255)
                                                    tGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATaa                                                                                                   >  2:86055/1‑150 (MQ=255)
                                                    tGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATaa                                                                                                   >  1:2482685/1‑150 (MQ=255)
                                                        gccCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAAccc                                                                                               >  1:2763371/1‑150 (MQ=255)
                                                          ccACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCgg                                                                                             >  1:1150233/1‑150 (MQ=255)
                                                              tGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGc                                                                                         >  1:4054992/1‑150 (MQ=255)
                                                                 aTTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTa                                                                                      >  2:1354155/1‑150 (MQ=255)
                                                                 aTTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGAATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTa                                                                                      >  2:2719259/1‑150 (MQ=255)
                                                                     gCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAac                                                                                  >  2:447323/1‑150 (MQ=255)
                                                                      ccAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAaca                                                                                 >  1:348653/1‑150 (MQ=255)
                                                                       cAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAacac                                                                                >  2:1098700/1‑150 (MQ=255)
                                                                       cAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAacac                                                                                >  1:4223850/1‑150 (MQ=255)
                                                                        aGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGAATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACg                                                                               >  1:1593410/1‑150 (MQ=255)
                                                                            gAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGctct                                                                           >  2:2967000/1‑150 (MQ=255)
                                                                              aCGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTa                                                                         <  2:2074659/150‑1 (MQ=255)
                                                                                       aTGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTcg                                                                >  2:426432/1‑150 (MQ=255)
                                                                                         gttgttACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTcgcg                                                              <  2:3656944/150‑1 (MQ=255)
                                                                                              tACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGAATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCAt                                                         <  1:811265/150‑1 (MQ=255)
                                                                                                  accaATCAGATGGCCCGCCGCGTCTTTATATTCCGGAGTCGCACCAACCCGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCt                                                     <  1:529876/150‑1 (MQ=255)
                                                                                                    caATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCCTCGCTGa                                                   >  2:2666473/1‑150 (MQ=255)
                                                                                                    caATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGa                                                   >  2:4760427/1‑150 (MQ=255)
                                                                                                    caATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGa                                                   >  2:1951174/1‑150 (MQ=255)
                                                                                                     aaTCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGAc                                                  <  2:348537/150‑1 (MQ=255)
                                                                                                        cAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGAATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGt                                               <  2:4704294/150‑1 (MQ=255)
                                                                                                         aGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTa                                              >  2:3097484/1‑150 (MQ=255)
                                                                                                         aGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTa                                              <  2:1871538/150‑1 (MQ=255)
                                                                                                         aGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTa                                              >  1:1860813/1‑150 (MQ=255)
                                                                                                         aGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTa                                              >  1:4070338/1‑150 (MQ=255)
                                                                                                              gCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAaagaa                                         <  2:2584836/150‑1 (MQ=255)
                                                                                                               cccGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAaagaag                                        >  2:2689736/1‑150 (MQ=255)
                                                                                                                    cgcgTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCAt                                   >  2:2772330/1‑150 (MQ=255)
                                                                                                                      cgTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCa                                 >  2:3934710/1‑150 (MQ=255)
                                                                                                                       gTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAg                                >  1:2246495/1‑150 (MQ=255)
                                                                                                                       gTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAg                                >  1:2390110/1‑150 (MQ=255)
                                                                                                                        tCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGa                               >  1:1471403/1‑150 (MQ=255)
                                                                                                                         cTTTATATTCCGGATTCGCACCAACCAGAATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGaa                              >  1:4149737/1‑150 (MQ=255)
                                                                                                                          tttATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTCAAGAAGCGCATCAGAAt                             >  2:1756220/1‑150 (MQ=255)
                                                                                                                           ttATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAAtg                            >  1:4549033/1‑150 (MQ=255)
                                                                                                                            tataTTCCGGATTCGCACCAACCAGAATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAAtgt                           <  1:104321/150‑1 (MQ=255)
                                                                                                                            tataTTCCGGATTCGCACCAACCAGAATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAAtgt                           >  1:902302/1‑150 (MQ=255)
                                                                                                                             atatTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAAtgtg                          <  2:3746308/150‑1 (MQ=255)
                                                                                                                              tatTCCGGATTCGCACCAACCAGAATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGc                         >  2:2319722/1‑150 (MQ=255)
                                                                                                                               atTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCg                        >  1:952006/1‑150 (MQ=255)
                                                                                                                                ttCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCgg                       <  1:3018096/150‑1 (MQ=255)
                                                                                                                                    ggATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTGGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAg                   >  2:4055648/1‑150 (MQ=255)
                                                                                                                                    ggATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAg                   <  2:1974119/150‑1 (MQ=255)
                                                                                                                                     gATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGc                  >  1:465229/1‑150 (MQ=255)
                                                                                                                                      aTTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGcc                 >  1:3912776/1‑150 (MQ=255)
                                                                                                                                       ttCGCACCAACCAGAATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGccc                <  1:1969081/150‑1 (MQ=255)
                                                                                                                                        tCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCccg               <  1:339487/150‑1 (MQ=255)
                                                                                                                                          gCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCccgcc             <  2:415691/150‑1 (MQ=255)
                                                                                                                                          gCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCccgcc             >  1:4534727/1‑150 (MQ=255)
                                                                                                                                            accaaccaGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGt           >  1:606245/1‑150 (MQ=255)
                                                                                                                                             ccaaccaGAATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTa          <  1:2261734/150‑1 (MQ=255)
                                                                                                                                                accaGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTACCa       <  2:4277843/150‑1 (MQ=255)
                                                                                                                                                 ccaGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTACCaa      >  1:3850869/1‑150 (MQ=255)
                                                                                                                                                  caGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTACCaaa     <  1:3808174/150‑1 (MQ=255)
                                                                                                                                                   aGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTACCAAAc    >  2:3508773/1‑150 (MQ=255)
                                                                                                                                                   aGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTACCAAAc    <  1:1389979/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACCGCGAGGATAGTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTACCAAACAT  >  CP009273/4273719‑4274017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑