Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,382,270 C→A 5.7% intergenic (+18/‑19) glyV → / → glyX tRNA‑Gly/tRNA‑Gly

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,382,2700CA 5.7% 461.3 / 6.3 139intergenic (+18/‑19)glyV/glyXtRNA‑Gly/tRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base C (46/83);  new base A (6/3);  total (53/86)
Fisher's exact test for biased strand distribution p-value = 8.09e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 144 total aligned reads displayed.

GCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTG  >  CP009273/4382122‑4382417
                                                                                                                                                     |                                                                                                                                                   
  cTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCaaa                                                                                                                                                  >  1:108863/1‑150 (MQ=255)
  cTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTAGCGAGGT‑CGACTCTCGTTTACCCCACCAAAAATAGAAAAGTGCTGCaaa                                                                                                                                                  >  1:109600/1‑150 (MQ=25)
   tCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAg                                                                                                                                                 <  2:4376532/150‑1 (MQ=255)
   tCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAg                                                                                                                                                 <  2:2473666/150‑1 (MQ=255)
   tCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAg                                                                                                                                                 <  2:2473719/150‑1 (MQ=255)
         aaCGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGa                                                                                                                                           <  2:4192716/150‑1 (MQ=255)
         aaCGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGa                                                                                                                                           <  2:4190722/150‑1 (MQ=255)
         aaCGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGa                                                                                                                                           <  2:4190707/150‑1 (MQ=255)
           cgacgCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGacc                                                                                                                                         <  1:4816674/150‑1 (MQ=255)
                        cGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCCAGCGGGac                                                                                                                            >  2:4607398/1‑149 (MQ=255)
                        cGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGaa                                                                                                                            <  1:3406364/150‑1 (MQ=255)
                          aaaaTTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATa                                                                                                                          <  2:2386811/150‑1 (MQ=255)
                             aTTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCt                                                                                                                       <  1:4315969/150‑1 (MQ=255)
                             aTTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCt                                                                                                                       <  1:4315695/150‑1 (MQ=255)
                              ttACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTc                                                                                                                      >  2:3217318/1‑150 (MQ=255)
                                aCGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAg                                                                                                                    <  2:774034/150‑1 (MQ=255)
                                 cGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGt                                                                                                                   <  1:2365252/150‑1 (MQ=255)
                                 cGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATATGAACAGTGATGCAACGCACCGACCACCCAACCGGGAATAgggaaga                                                                                                                   >  2:2047057/1‑144 (MQ=17)
                                   aaaGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAACCCGGAAAAGCACAGTTg                                                                                                                 >  1:4007364/1‑150 (MQ=255)
                                    aaGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTgg                                                                                                                <  2:566021/150‑1 (MQ=255)
                                     aGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGt                                                                                                               <  2:2688766/150‑1 (MQ=255)
                                     aGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGt                                                                                                               <  2:2929705/150‑1 (MQ=255)
                                     aGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGt                                                                                                               <  2:3527863/150‑1 (MQ=255)
                                       cAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTTAAAAGACAATAAAAGCACATACCACCCAAGCGGGAATAGCTCAGTTGGTag                                                                                                             >  1:3432862/1‑150 (MQ=14)
                                       cAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTTCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTag                                                                                                             >  1:1305300/1‑150 (MQ=255)
                                              aaGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCATGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATATGAAAAGTGATGAAAAGCACAGACCACCCCAGCGGGAATAGCTCAGTTGGTAGAGCACGa                                                                                                      >  2:3852035/1‑150 (MQ=14)
                                              aaGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATATGAAATCTCATTAAAAGCAACACCCACCCAAGCCGTAATAGCGCATTTGGTaggtctaga                                                                                                      >  2:2405424/1‑143 (MQ=14)
                                               agtagtACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGAc                                                                                                     >  1:4708848/1‑150 (MQ=255)
                                                 tagtaCGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCt                                                                                                   <  1:3854842/150‑1 (MQ=255)
                                                 tagtaCGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCt                                                                                                   <  1:1316834/150‑1 (MQ=255)
                                                  agtaCGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCC‑AAATTTGACAAGTGCTGCAAAGCACAGACCACCCAAGAGGGAATAGCGAAGTTGGTAGAGCACGACCTTg                                                                                                 >  2:2163311/1‑150 (MQ=14)
                                                   gtaCGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTg                                                                                                 <  2:3873002/150‑1 (MQ=255)
                                                     aCGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCTCGACCTTGcc                                                                                               <  2:628379/150‑1 (MQ=255)
                                                     aCGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGGTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCTCGACCTTGcc                                                                                               <  2:628361/150‑1 (MQ=255)
                                                        cgGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAg                                                                                            <  1:3035600/150‑1 (MQ=39)
                                                        cgGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAg                                                                                            <  1:2274917/150‑1 (MQ=39)
                                                           gAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTc                                                                                         <  1:3871977/150‑1 (MQ=39)
                                                               acaaactttggttggGCACTCCCTTGCCAAGGAGGGGGAGGCTATTT‑CGAGTCACGTTTCCC‑CACCAAAATTTTTAAAGTGCTGAAACGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGt                                                                                    <  2:4160066/143‑1 (MQ=2)
                                                                 cTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCTCGACCTTGCCAAGGTCGGGGTc                                                                                   <  1:2385695/150‑1 (MQ=255)
                                                                  tCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTcg                                                                                  <  2:3946726/150‑1 (MQ=39)
                                                                    aGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTcgcg                                                                                >  1:987380/1‑150 (MQ=39)
                                                                        ggTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCTGTTGGTCGAGCACGACCTTGCCCCGGTCGGGGTCGCGCGtt                                                                            >  2:3899503/1‑150 (MQ=14)
                                                                         gTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAATTTTGAAAACTGATTCAAAGCACAGACCACCCAAGCGGGACTAGCTCTGTTGGTAGAGCTCGTCCTAGCCCAGGTCGGGGGCGCGAGTTc                                                                           >  2:3591443/1‑150 (MQ=14)
                                                                             agCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAga                                                                       >  2:3289791/1‑149 (MQ=39)
                                                                               cACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGtct                                                                     <  2:374733/150‑1 (MQ=39)
                                                                                  gACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGt                                                                  <  2:395274/150‑1 (MQ=39)
                                                                                    ccTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCGCAGTTGGTAGAGCACGACCTTGCCCAGGTCGGGGTCGCGAGTTCGAGTCTCGttt                                                                >  2:408410/1‑150 (MQ=255)
                                                                                     cTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTATAGCTCTACCTTGCCAAGGTCGGGGGCGCGAGTTCGAGTCTCGTTTc                                                               >  2:2866228/1‑150 (MQ=255)
                                                                                     cTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCTCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTc                                                               >  1:767596/1‑150 (MQ=255)
                                                                                     cTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTc                                                               <  1:2149084/150‑1 (MQ=39)
                                                                                       tGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTccc                                                             >  1:4459460/1‑150 (MQ=39)
                                                                                          cAAGGTCGGGGTCGCGTTTT‑CGATTCTCGTTTCCCGCTCCAAAATTTTAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCt                                                          <  2:2275401/150‑1 (MQ=255)
                                                                                           taGGTAGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAATTTCTGCAAAGCACAGAACACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTc                                                         <  2:215356/149‑1 (MQ=255)
                                                                                           aaGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTc                                                         >  2:2874994/1‑150 (MQ=39)
                                                                                           aaGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTc                                                         <  2:123352/150‑1 (MQ=39)
                                                                                            aGGTCGGGGGCGCGTCTTGCGGGTCCGGTTTCCCCCACCAAAA‑TTGAAAAGTGCTTCAAGGCACAGACCACCCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTcc                                                        <  2:2671849/150‑1 (MQ=11)
                                                                                             ggTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCTCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCa                                                       <  1:2005418/150‑1 (MQ=255)
                                                                                              gTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCaa                                                      <  2:1858686/150‑1 (MQ=39)
                                                                                                 ggggTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTTCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAAt                                                   <  2:3953052/150‑1 (MQ=255)
                                                                                                 ggggTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCaaatt                                                   >  2:1394821/1‑148 (MQ=38)
                                                                                                 ggggTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAAt                                                   >  2:1396351/1‑150 (MQ=40)
                                                                                                    gTCGCGAGTT‑CGAGTCTCGGTTCCCGCTCCAAAATTTGAAAAGTGCTGCACAGCACAGACCTCCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTg                                                <  2:2398581/150‑1 (MQ=255)
                                                                                                       gcgAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGaaa                                             <  1:3116279/150‑1 (MQ=255)
                                                                                                       gcgAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGaaa                                             <  1:291664/150‑1 (MQ=255)
                                                                                                        cgtgTT‑CGTGTCTCGTTTCCGTCTCCTAAATTTGAAAATTGCTTCAGAGCACAGACCTCGGAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAg                                            <  2:4026992/147‑1 (MQ=14)
                                                                                                        cgAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAg                                            <  2:4028673/150‑1 (MQ=255)
                                                                                                          aGTT‑CGAGTCTCGTTTCCCTCTCCAAAATTTGAAAAGTGCTGCAACTCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTg                                          <  2:3357634/150‑1 (MQ=255)
                                                                                                          aGTT‑CGAGTCTCGGTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTg                                          <  2:3357809/150‑1 (MQ=255)
                                                                                                            tt‑CGTGTCTCGTTTCCCGCTCCAAAATGTGAAAAGTGCTGCATATCACAGACCACCCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCt                                        <  1:1071154/150‑1 (MQ=17)
                                                                                                            tt‑CGAGTCTCGTTTCCCGCTCCTAAATTTGAAAAGTGCTGCAAAGCACAGAACAAGCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCt                                        <  1:4004151/150‑1 (MQ=255)
                                                                                                             t‑CGAGTCTCGTTTCCCGCTCCTAAATTTGAAAAGTTCTTCAAAGCACTGAACACCCAAGCGGGAATAGCTCAGTTGGTAGAGCTCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTg                                       <  1:406049/150‑1 (MQ=17)
                                                                                                             t‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCACGCGGGAATAGCTCAGTTGGTAGAGCTCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTg                                       <  1:3577/150‑1 (MQ=255)
                                                                                                             t‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTg                                       >  1:2936818/1‑150 (MQ=255)
                                                                                                               cGAGTCTCGTTTCCCTCTCCAAAATGTGAAAAGTGCTGCAAAGCACAGACCAACAAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGt                                      <  2:3617502/150‑1 (MQ=255)
                                                                                                               cGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCTCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGt                                      <  2:1109580/150‑1 (MQ=255)
                                                                                                               cGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTATGaaatgtatgt                                      >  2:3965752/1‑143 (MQ=255)
                                                                                                                  gTCTCGTTTCCCTCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAg                                   <  2:2982349/150‑1 (MQ=255)
                                                                                                                  gTCTCGTTTCCCGCTCAAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAg                                   <  2:1625656/150‑1 (MQ=255)
                                                                                                                     tcGTTTCCGTCTCATAAATTTGAAAAGTTCTTAAAAGAACATACCAAGCAAGCGGGAATAGCTCAGTTGGTAGAGCTCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGca                                <  1:1654967/150‑1 (MQ=17)
                                                                                                                     tcGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCTCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGca                                <  1:3881306/150‑1 (MQ=255)
                                                                                                                     tcGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCTCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGca                                <  1:1655008/150‑1 (MQ=255)
                                                                                                                     tcGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATGTGAAAGAACTGTaatgga                                >  1:4221390/1‑146 (MQ=255)
                                                                                                                       gTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGcaca                              >  2:1729515/1‑150 (MQ=255)
                                                                                                                         ttCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATATGAAAGTGCTGAAAGGAACAGa                            >  2:2084261/1‑150 (MQ=255)
                                                                                                                           acggCTCAAAAATTTGAAAATTACTCAAAATCAAAGAACTCGGACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGacc                          <  2:3297866/147‑1 (MQ=14)
                                                                                                                            ccGCTCCAAAATGTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGacca                         <  2:559231/150‑1 (MQ=255)
                                                                                                                               ctcataaaTTTGAAAATTTCTTCACAGCACATAACTCGCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCAccc                      <  1:1854853/145‑1 (MQ=17)
                                                                                                                               cTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCAccc                      <  1:4307878/150‑1 (MQ=255)
                                                                                                                               cTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCAccc                      <  2:4459460/150‑1 (MQ=255)
                                                                                                                                tCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGGGCTGTAAGGAACAGACCGCCCa                     >  1:1729985/1‑150 (MQ=255)
                                                                                                                                   aaaaTTTGAAAAGTGCTTCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGc                  <  2:156425/150‑1 (MQ=255)
                                                                                                                                        ttGAAAAGTGCTGCATAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGaa             <  1:743662/150‑1 (MQ=255)
                                                                                                                                         tGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAAt            <  2:4708848/150‑1 (MQ=255)
                                                                                                                                          gAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATa           <  1:365593/150‑1 (MQ=255)
                                                                                                                                           aaaaGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAg          >  1:4528340/1‑150 (MQ=255)
                                                                                                                                           aaaaGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAg          >  1:2573009/1‑150 (MQ=255)
                                                                                                                                           aaaaGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAg          >  1:2571917/1‑150 (MQ=255)
                                                                                                                                           aaaaGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGGTCGAGTCTCGTTTCCCGCTCCAAAATCTGAACGCGCTCTAAGCACCAGACCGCCCCGTCGGGACTAg          >  2:3476655/1‑150 (MQ=14)
                                                                                                                                                  ctgcAAATCACAGACCACCCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGtt   <  2:965556/150‑1 (MQ=255)
                                                                                                                                                   tgcAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTg  <  1:2875116/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTG  >  CP009273/4382122‑4382417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑