Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,382,314 A→T 18.2% noncoding (26/76 nt) glyX → tRNA‑Gly

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,382,3140AT18.2% 372.6 / 76.2 156noncoding (26/76 nt)glyXtRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base A (56/71);  new base T (14/14);  total (71/85)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 156 total aligned reads displayed.

TAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTC  >  CP009273/4382167‑4382460
                                                                                                                                                    |                                                                                                                                                  
tAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATATGAAAA‑TGGGGTAAGGCACATACCACCCAACCCGGAAAAGCGCAGTTGGTAGAGCAAGa                                                                                                                                                 >  1:3810156/1‑150 (MQ=11)
  agtagtACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGAc                                                                                                                                                >  1:4708848/1‑150 (MQ=255)
   gtagtaCGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGAcc                                                                                                                                               <  2:1482071/150‑1 (MQ=255)
    tagtaCGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCt                                                                                                                                              <  1:3854842/150‑1 (MQ=255)
     agtaCGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCC‑AAATTTGACAAGTGCTGCAAAGCACAGACCACCCAAGAGGGAATAGCGAAGTTGGTAGAGCACGACCTTg                                                                                                                                            >  2:2163311/1‑150 (MQ=14)
      gtaCGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTg                                                                                                                                            <  2:4420313/150‑1 (MQ=255)
      gtaCGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTg                                                                                                                                            <  2:3873002/150‑1 (MQ=255)
        aCGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCTCGACCTTGcc                                                                                                                                          <  2:628325/150‑1 (MQ=255)
        aCGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGGTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCTCGACCTTGcc                                                                                                                                          <  2:628361/150‑1 (MQ=255)
           cgGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAg                                                                                                                                       <  1:2274917/150‑1 (MQ=39)
             ggAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAACTGCTAAAATTCACACACCACCCAAGCGGGAATAGCTCAGTTGGTAGTGCACGACCTTGCCAAGGt                                                                                                                                     >  2:129141/1‑150 (MQ=11)
                  acaaactttggttggGCACTCCCTTGCCAAGGAGGGGGAGGCTATTT‑CGAGTCACGTTTCCC‑CACCAAAATTTTTAAAGTGCTGAAACGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGt                                                                                                                               <  2:4160066/143‑1 (MQ=2)
                    cTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCTCGACCTTGCCAAGGTCGGGGTc                                                                                                                              <  1:2385695/150‑1 (MQ=255)
                     tCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTcg                                                                                                                             <  2:3946726/150‑1 (MQ=39)
                       aGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTcgcg                                                                                                                           >  1:987380/1‑150 (MQ=39)
                           ggTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGATGCACAGCCCCGCCAACCCAGGGGGGAATAGCTCGGGTGGTGGAGAGCGCCGTGGCCAGGGTGGGGGTCGCGAGtg                                                                                                                       >  2:3899142/1‑149 (MQ=14)
                            gTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAATTTTGAAAACTGATTCAAAGCACAGACCACCCAAGCGGGACTAGCTCTGTTGGTAGAGCTCGTCCTAGCCCAGGTCGGGGGCGCGAGTTc                                                                                                                      >  2:3591443/1‑150 (MQ=14)
                                agCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAga                                                                                                                  >  2:3289791/1‑149 (MQ=39)
                                  cACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGtct                                                                                                                <  2:374733/150‑1 (MQ=39)
                                     gACCTGGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCACGTTTCCCGCTCCTAAATTTGAAAAGTGCTGCAACGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGt                                                                                                             <  2:395209/150‑1 (MQ=255)
                                       ccTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCGCAGTTGGTAGAGCACGACCTTGCCCAGGTCGGGGTCGCGAGTTCGAGTCTCGttt                                                                                                           >  2:408410/1‑150 (MQ=255)
                                        cTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTATAGCTCTACCTTGCCAAGGTCGGGGGCGCGAGTTCGAGTCTCGTTTc                                                                                                          >  2:2866228/1‑150 (MQ=255)
                                        cTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCTCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTc                                                                                                          >  1:767596/1‑150 (MQ=255)
                                             cAAGGTCGGGGTCGCGTTTT‑CGATTCTCGTTTCCCGCTCCAAAATTTTAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCt                                                                                                     <  2:2275401/150‑1 (MQ=255)
                                              taGGTAGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAATTTCTGCAAAGCACAGAACACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTc                                                                                                    <  2:215356/149‑1 (MQ=255)
                                               aGGTCGGGGGCGCGTCTTGCGGGTCCGGTTTCCCCCACCAAAA‑TTGAAAAGTGCTTCAAGGCACAGACCACCCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTcc                                                                                                   <  2:2671849/150‑1 (MQ=11)
                                                ggTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCTCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCa                                                                                                  <  1:4457999/150‑1 (MQ=255)
                                                ggTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCTCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCa                                                                                                  <  1:2005418/150‑1 (MQ=255)
                                                 gTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCaa                                                                                                 <  2:1858686/150‑1 (MQ=39)
                                                    ggggTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTTCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAAt                                                                                              <  2:3953052/150‑1 (MQ=255)
                                                    ggggTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCaaatt                                                                                              >  2:1394821/1‑148 (MQ=38)
                                                    ggggTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAAt                                                                                              >  2:1396351/1‑150 (MQ=40)
                                                       gTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTGGAAAAGTGCTGCAAATCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTg                                                                                           <  2:2398694/150‑1 (MQ=255)
                                                          gcgAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGaaa                                                                                        <  1:3116279/150‑1 (MQ=255)
                                                           cgtgTT‑CGTGTCTCGTTTCCGTCTCCTAAATTTGAAAATTGCTTCAGAGCACAGACCTCGGAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAg                                                                                       <  2:4026992/147‑1 (MQ=14)
                                                             aGTT‑CGAGTCTCGTTTCCCTCTCCAAAATTTGAAAAGTGCTGCAACTCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTg                                                                                     <  2:3357634/150‑1 (MQ=255)
                                                             aGTT‑CGAGTCTCGGTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTg                                                                                     <  2:3357809/150‑1 (MQ=255)
                                                               tt‑CGTGTCTCGTTTCCCGCTCCAAAATGTGAAAAGTGCTGCATATCACAGACCACCCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCt                                                                                   <  1:1071154/150‑1 (MQ=17)
                                                                t‑CGAGTCTCGTTTCCCGCTCCTAAATTTGAAAAGTTCTTCAAAGCACTGAACACCCAAGCGGGAATAGCTCAGTTGGTAGAGCTCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTg                                                                                  <  1:406049/150‑1 (MQ=17)
                                                                t‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCACGCGGGAATAGCTCAGTTGGTAGAGCTCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTg                                                                                  <  1:3577/150‑1 (MQ=255)
                                                                t‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTg                                                                                  >  1:2936818/1‑150 (MQ=255)
                                                                  cGAGTCTCGTTTCCCGCTCCAGAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGt                                                                                 >  2:3965516/1‑150 (MQ=255)
                                                                  cGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCTCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGt                                                                                 <  2:1109580/150‑1 (MQ=255)
                                                                     gTCTCGTTTCCCGCTCAAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAg                                                                              <  2:1625656/150‑1 (MQ=255)
                                                                        tcGTTTCCGTCTCATAAATTTGAAAAGTTCTTAAAAGAACATACCAAGCAAGCGGGAATAGCTCAGTTGGTAGAGCTCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGca                                                                           <  1:1654967/150‑1 (MQ=17)
                                                                        tcGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCTCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGca                                                                           <  1:1655008/150‑1 (MQ=255)
                                                                        tcGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCTCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGca                                                                           <  1:3881306/150‑1 (MQ=255)
                                                                        tcGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATGTGAAAGAACTGTaatgga                                                                           >  1:4221390/1‑146 (MQ=255)
                                                                          gTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGcaca                                                                         >  2:1729515/1‑150 (MQ=255)
                                                                              acggCTCAAAAATTTGAAAATTACTCAAAATCAAAGAACTCGGACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGacc                                                                     <  2:3297866/147‑1 (MQ=14)
                                                                               ccGCTCCAAAATGTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGacca                                                                    <  2:559231/150‑1 (MQ=255)
                                                                                  cTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCAccc                                                                 <  1:4307878/150‑1 (MQ=255)
                                                                                   tCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCa                                                                >  1:2410413/1‑150 (MQ=255)
                                                                                   tCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGGGCTGTAAGGAACAGACCGCCCa                                                                >  1:1729985/1‑150 (MQ=255)
                                                                                       aaaTTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCg                                                            >  1:2841829/1‑150 (MQ=255)
                                                                                         aTTTGAAAAGTGCTTCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCggg                                                          <  2:2767188/150‑1 (MQ=255)
                                                                                           ttGAAAAGTGCTGCATAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGaa                                                        <  1:743662/150‑1 (MQ=255)
                                                                                            tGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAAt                                                       >  2:2554168/1‑150 (MQ=255)
                                                                                            tGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAAt                                                       <  2:4708848/150‑1 (MQ=255)
                                                                                             gAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATa                                                      <  1:365593/150‑1 (MQ=255)
                                                                                              aaaaGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAg                                                     >  1:4528340/1‑150 (MQ=255)
                                                                                              aaaaGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAg                                                     >  1:2571917/1‑150 (MQ=255)
                                                                                              aaaaGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGGTCGAGTCTCGTTTCCCGCTCCAAAATCTGAACGCGCTCTAAGCACCAGACCGCCCCGTCGGGACTAg                                                     >  2:3476655/1‑150 (MQ=14)
                                                                                                  gTGCTGCAAATCACAGACCACCCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCa                                                 <  2:2903288/150‑1 (MQ=255)
                                                                                                     ctgcAAATCACAGACCACCCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGtt                                              <  2:965556/150‑1 (MQ=255)
                                                                                                     ctgcAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGtt                                              <  1:719286/150‑1 (MQ=255)
                                                                                                     ctgcAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATATGAAAGAGCTATAATGAACAGACCACCCCAGAGGGAATAGCGCCGtt                                              >  1:2949997/1‑150 (MQ=17)
                                                                                                      tgcAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTg                                             <  1:2875116/150‑1 (MQ=255)
                                                                                                         aaaGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCGCAGTTGGtc                                          >  1:3253489/1‑149 (MQ=255)
                                                                                                          tacaacaGACCACCTAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTag                                         <  1:543230/146‑1 (MQ=255)
                                                                                                           aGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCTCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGAACAGACCACCCAAGCGGGAATAGCTCAGTTGGTcga                                        >  1:1389175/1‑150 (MQ=255)
                                                                                                           aGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCTCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATGTGAAATGGCTGTAAGGCACAGACCCCCCAACAGGGAAAAGCTGAGTTGGTaga                                        >  2:1035159/1‑150 (MQ=17)
                                                                                                            gCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTagag                                       <  1:4607398/150‑1 (MQ=255)
                                                                                                             cacaGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAATTGCTGTAAGGCACAGACCACCCAATCGGGAATAGCTCAGTTGGTAGAGc                                      >  1:896785/1‑150 (MQ=255)
                                                                                                             cacaGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGAACAGACCACCCAAGCGGGAATAGCTCAGGTGGTAGAGc                                      >  1:4802296/1‑150 (MQ=255)
                                                                                                               aatacctccCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCAc                                    <  2:123146/147‑1 (MQ=255)
                                                                                                                aGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCTCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCCGTTGTTAGAGCACg                                   >  2:3992100/1‑150 (MQ=255)
                                                                                                                aGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAATGCACAGACCACCCACGCGGGAATAGCGAGGTTGGGAGAGCACg                                   >  2:1629297/1‑150 (MQ=14)
                                                                                                                aGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATGTGAAAGTGCTGTAAGGCACAGACCCCCCAAGCGGGAATAGCTCGGTTGGTAGAGCACg                                   >  2:1628486/1‑150 (MQ=255)
                                                                                                                  accaccCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGAGCTTTAAGGCACAGACCACCCCAGCGGGAATATCTCAGTTGGTAGAGAACGAc                                 >  2:2974675/1‑150 (MQ=14)
                                                                                                                   ccaccCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGAcc                                >  1:3381909/1‑150 (MQ=255)
                                                                                                                    caccCCCGCGGGAATTGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCt                               <  2:1967152/150‑1 (MQ=255)
                                                                                                                     accCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCtt                              >  2:1238310/1‑150 (MQ=255)
                                                                                                                       ccAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATATCTCAGTTGGTAGAGCACGACCTTGc                            >  1:1283663/1‑150 (MQ=255)
                                                                                                                        cAAGCGGGAATAGCTCAGTTGGTAGAGCTCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCACCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGcc                           <  1:1867990/150‑1 (MQ=255)
                                                                                                                            cGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGACCTGTAAGGCACACACCACCCCCGCGGGAATATCTCAGTTTGTAGAGCACGACCATTACAAgg                       >  1:3427423/1‑150 (MQ=11)
                                                                                                                            cGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGAGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAgg                       <  1:2617933/150‑1 (MQ=38)
                                                                                                                             gggAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGt                      >  1:3303216/1‑150 (MQ=38)
                                                                                                                              ggAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGATTTCGAGTCTCGTTTCCCGCTCCAAACTTTGAAAGTGCTGTAAGGCACAGAACACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTc                     <  1:4851912/150‑1 (MQ=255)
                                                                                                                                aaTAGCTCAGTTGGTAGAGCTCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATATTAAATAGATGAAATGAACAGACCCCCCACGCGGGAATATCGCAGTTTGTAGAGCAAGACCAAGCCAAGTTCgc                   >  1:196544/1‑149 (MQ=11)
                                                                                                                                aaTAGCTCAGTTGGTAGAGCACGACCTTGCTAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATGTGAAAGAGCTGAAATAAACAGACCACCCCAACGGGAAAAGCGCAGTTTGTATAGAACCACCGTCACAAGGTAgg                   >  1:4789893/1‑150 (MQ=11)
                                                                                                                                  tAGCTCAGTTGGTAGAGCTCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCgggg                 >  1:1339782/1‑150 (MQ=38)
                                                                                                                                  tAGCTCAGTTGGTAGAGCTCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCgggg                 >  1:1023858/1‑150 (MQ=38)
                                                                                                                                   aGCTCAGTTGGTAGAGCTCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTTTTAAAGAGATGTAAGGCACAGACCACCCAAGAGGGAATAGCTCGGTTGGTAGAGAACGACCTAGCCAAGGGAGGGGt                >  2:1798648/1‑150 (MQ=11)
                                                                                                                                   aGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGt                <  1:1939591/150‑1 (MQ=38)
                                                                                                                                    gCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGATTTCGAGTCTCGTTTCCCGCTCCAAAATTTTAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTc               <  1:2152912/150‑1 (MQ=255)
                                                                                                                                       cAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTcgcg            >  1:1165005/1‑150 (MQ=38)
                                                                                                                                               agagCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTGGGTAGAGCACGACCGTGCCAAGGTCGGGGTCGCGAGTTCGAg    >  1:2574209/1‑150 (MQ=255)
                                                                                                                                                gagCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGt   <  1:876633/150‑1 (MQ=38)
                                                                                                                                                 agCTCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGtc  >  2:1661391/1‑150 (MQ=38)
                                                                                                                                                    |                                                                                                                                                  
TAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTT‑CGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTC  >  CP009273/4382167‑4382460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑