BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,400,254	C→A	19.7%	S102* (TCG→TAG)	yjfI →	DUF2170 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,400,254	0	C	A	19.7%	584.0 / 114.3	213	S102* (TCG→TAG)	yjfI	DUF2170 family protein
Reads supporting (aligned to +/- strand): ref base C (76/95); new base A (18/24); total (94/119)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

Only 100 of 215 total aligned reads displayed.

CGAAACCTTTATTTGTCCGGTGAGTAGTATCAGCAATCCAGATGAATTTAATACCTTCTTATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGA  >  CP009273/4400105‑4400403

cGAAACCTTTATTTGTCCGGTGAGTAGTATCAGCAATCCAGATGAATTTAATACCTTCTTATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATc                                                                                                                                                       >  1:590316/1‑150 (MQ=255)

      cTTTATTTGTCCGGTGAGTAGTATCAGCAATCCAGATGAATTTAATACCTTCTTATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTaa                                                                                                                                                 >  2:2715447/1‑150 (MQ=255)

      cTTTATTTGTCCGGTGAGTAGTATCAGCAATCCAGATGAATTTAATACCTTCTTATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATAGCTTaa                                                                                                                                                 <  1:336369/150‑1 (MQ=255)

        ttATTTGTCCGGTGAGTAGTATCAGCAATCCAGATGAATTTAATACCTTCTTATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAAt                                                                                                                                               <  1:4148647/150‑1 (MQ=255)

          aTTTGTCCGGTGAGTAGTATCAGCAATCCAGATGAATTTAATACCTTCTTATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAAtct                                                                                                                                             <  2:221411/150‑1 (MQ=255)

          aTTTGTCCGGTGAGTAGTATCAGCAATCCAGATGAATTTAATACCTTCTTATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAAtct                                                                                                                                             <  2:234698/150‑1 (MQ=255)

          aTTTGTCCGGTGAGTAGTATCAGCAATCCAGATGAATTTAATACCTTCTTATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAAtct                                                                                                                                             >  2:2182973/1‑150 (MQ=255)

          aTTTGTCCGGTGAGTAGTATCAGCAATCCAGATGAATTTAATACCTTCTTATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAAtct                                                                                                                                             >  2:2460472/1‑150 (MQ=255)

          aTTTGTCCGGTGAGTAGTATCAGCAATCCAGATGAACTTAATACCTTCTTATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAAtct                                                                                                                                             >  2:2186074/1‑150 (MQ=255)

           tttGTCCGGTGAGTAGTATCAGCAATCCAGATGAATTTAATACCTTCTTATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAAtctt                                                                                                                                            >  1:4226579/1‑150 (MQ=255)

            ttGTCCGGTGAGTAGTATCAGCAATCCAGATGAATTTAATACCTTCTTATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAAtcttc                                                                                                                                           >  2:16089/1‑150 (MQ=255)

               tCCGGTGAGTAGTATCAGCAATCCAGATGAATTTAATACCTTCTTATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATAGCTTAAATCTTctct                                                                                                                                        <  1:3124231/150‑1 (MQ=255)

                ccGGTGAGTAGTATCAGCAATCCAGATGAATTTAATACCTTCTTATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATAGCTTAAATCTTCTCtt                                                                                                                                       >  1:3805342/1‑150 (MQ=255)

                  ggTGAGTAGTATCAGCAATCCAGATGAATTTAATACCTTCTTATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGa                                                                                                                                     >  1:4836847/1‑150 (MQ=255)

                   gTGAGTAGTATCAGCAATCCAGATGAATTTAATACCTTCTTATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGaa                                                                                                                                    <  2:1394533/150‑1 (MQ=255)

                   gTGAGTAGTATCAGCAATCCAGATGAATTTAATACCTTCTTATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGaa                                                                                                                                    <  1:1668441/150‑1 (MQ=255)

                      agtagtATCAGCAATCCAGATGAATTTAATACCTTCTTATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATAGCTTAAATCTTCTCTTGAAGat                                                                                                                                 >  1:684261/1‑150 (MQ=255)

                       gtagtaTCAGCAATCCAGATGAATTTAATACCTTCTTATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGata                                                                                                                                >  1:1346861/1‑150 (MQ=255)

                               aGCAATCCAGATGAATTTAATACCTTCTTATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCtt                                                                                                                        <  2:1487206/150‑1 (MQ=255)

                               aGCAATCCAGATGAATTTAATACCTTCTTATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCtt                                                                                                                        <  2:3975846/150‑1 (MQ=255)

                                 cAATCCAGATGAATTTAATACCTTCTTATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTga                                                                                                                      >  2:1441631/1‑150 (MQ=255)

                                  aaTCCAGATGAATTTAATACCTTCTTATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTgag                                                                                                                     <  1:2947529/150‑1 (MQ=255)

                                   aTCCAGATGAATTTAATACCTTCTTATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTgaga                                                                                                                    >  2:2382786/1‑150 (MQ=255)

                                     ccAGATGAATTTAATACCTTCTTATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATa                                                                                                                  <  1:358330/150‑1 (MQ=255)

                                     ccAGATGAATTTAATACCTTCTTATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATa                                                                                                                  <  2:852846/150‑1 (MQ=255)

                                     ccAGATGAATTTAATACCTTCTTATTAAGAAATCAGAAGATGATGCCGGTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATa                                                                                                                  <  2:852486/150‑1 (MQ=255)

                                       aGATGAATTTAATACCTTCTTATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAAc                                                                                                                >  2:2653561/1‑150 (MQ=255)

                                       aGATGAATTTAATACCTTCTTATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATAGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAAc                                                                                                                >  2:4616696/1‑150 (MQ=255)

                                        gATGAATTTAATACCTTCTTATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATAGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAAcc                                                                                                               <  1:179709/150‑1 (MQ=255)

                                         aTGAATTTAATACCTTCTTATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATAGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCt                                                                                                              >  2:1648621/1‑150 (MQ=255)

                                             aTTTAATACCTTCTTATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGGGATAACCTCGCt                                                                                                          <  1:1720332/150‑1 (MQ=255)

                                              tttAATACCTTCTTATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTg                                                                                                         <  1:3509439/150‑1 (MQ=255)

                                                 aaTACCTTCTTATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACACCAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTa                                                                                                      >  2:1343874/1‑150 (MQ=255)

                                                 aaTACCTTCTTATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTa                                                                                                      <  2:3613039/150‑1 (MQ=255)

                                                 aaTACCTTCTTATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTa                                                                                                      >  2:1371154/1‑150 (MQ=255)

                                                  aTACCTTCTTATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGATTGAGATAACCTCGCTGGTAg                                                                                                     >  2:4388874/1‑150 (MQ=255)

                                                    aCCTTCTTATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGAt                                                                                                   <  1:4609519/150‑1 (MQ=255)

                                                       ttcttATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAAc                                                                                                <  2:4836847/150‑1 (MQ=255)

                                                        tcttATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACg                                                                                               <  2:1272867/150‑1 (MQ=255)

                                                        tcttATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACg                                                                                               <  1:2763760/150‑1 (MQ=255)

                                                         cttATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGc                                                                                              <  1:540181/150‑1 (MQ=255)

                                                           tattaAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCAt                                                                                            <  1:2186055/150‑1 (MQ=255)

                                                           tattaAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCAt                                                                                            >  1:4319360/1‑150 (MQ=255)

                                                           tattaAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCAt                                                                                            <  1:2182973/150‑1 (MQ=255)

                                                           tattaAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCAt                                                                                            <  1:3754681/150‑1 (MQ=255)

                                                            attaAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCAtt                                                                                           >  2:2700244/1‑150 (MQ=255)

                                                            attaAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCAtt                                                                                           >  1:1840744/1‑150 (MQ=255)

                                                             ttaAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTg                                                                                          >  2:406007/1‑150 (MQ=255)

                                                             ttaAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTg                                                                                          >  2:283374/1‑150 (MQ=255)

                                                               aaGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGa                                                                                        <  1:1767516/150‑1 (MQ=255)

                                                                 gAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATc                                                                                      >  2:3059012/1‑150 (MQ=255)

                                                                  aaaTCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCt                                                                                     <  2:4230634/150‑1 (MQ=255)

                                                                  aaaTCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCt                                                                                     <  1:2700244/150‑1 (MQ=255)

                                                                   aaTCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTg                                                                                    >  1:3017037/1‑150 (MQ=255)

                                                                   aaTCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATAGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTg                                                                                    >  1:3914394/1‑150 (MQ=255)

                                                                      cAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCt                                                                                 >  2:1764762/1‑150 (MQ=255)

                                                                      cAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCt                                                                                 >  2:3105598/1‑150 (MQ=255)

                                                                      cAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCt                                                                                 >  2:671521/1‑150 (MQ=255)

                                                                      cAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATAGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCt                                                                                 >  2:4261624/1‑150 (MQ=255)

                                                                          agaTGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGaaa                                                                             >  2:4222330/1‑150 (MQ=255)

                                                                           gatgatGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAAt                                                                            <  1:3155750/150‑1 (MQ=255)

                                                                                   cGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACagaag                                                                    >  2:3518368/1‑150 (MQ=255)

                                                                                   cGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACagaag                                                                    >  2:2869217/1‑150 (MQ=255)

                                                                                    gTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACagaaga                                                                   >  1:3527214/1‑150 (MQ=255)

                                                                                      tGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAat                                                                 <  1:265939/150‑1 (MQ=255)

                                                                                       gTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAata                                                                <  2:1117499/150‑1 (MQ=255)

                                                                                        tCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATAGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAatat                                                               >  2:4191131/1‑150 (MQ=255)

                                                                                         cATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATAtt                                                              <  1:4699898/150‑1 (MQ=255)

                                                                                         cATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATAtt                                                              <  1:3048636/150‑1 (MQ=255)

                                                                                         cATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATAtt                                                              <  1:3048582/150‑1 (MQ=255)

                                                                                          aTCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTc                                                             >  1:4265838/1‑150 (MQ=255)

                                                                                          aTCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATAGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTc                                                             >  1:2202156/1‑150 (MQ=255)

                                                                                             ggTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTcaca                                                          <  1:669507/150‑1 (MQ=255)

                                                                                               tAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACt                                                        >  2:3228446/1‑150 (MQ=255)

                                                                                               tAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATAGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACt                                                        >  2:1799956/1‑150 (MQ=255)

                                                                                                   gATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAAtt                                                    <  2:1285849/150‑1 (MQ=255)

                                                                                                    aTCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTa                                                   <  1:697592/150‑1 (MQ=255)

                                                                                                          aGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGa                                             >  2:3404473/1‑150 (MQ=255)

                                                                                                            cGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACg                                           <  2:3017704/150‑1 (MQ=255)

                                                                                                              tACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATAGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACggg                                         <  1:873080/150‑1 (MQ=255)

                                                                                                               acaacaGGAAGAGTATTACATTGTTTTCGGGGCGTTATAGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGt                                        <  1:498593/150‑1 (MQ=255)

                                                                                                                caacaGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTa                                       <  1:2460472/150‑1 (MQ=255)

                                                                                                                 aacaGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGTCTGAAATTACAGAAGAATATTCACAATAATTACAAGGACGGGtct                                      >  2:2476326/1‑148 (MQ=255)

                                                                                                                 aacaGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTAt                                      >  2:2059268/1‑150 (MQ=255)

                                                                                                                   caGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTAttt                                    <  2:4483881/150‑1 (MQ=255)

                                                                                                                     ggAAGAGTATTACATTGTTTTCGGGGCGTTATAGCTTAAATATTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTAt                                  <  2:3718961/150‑1 (MQ=255)

                                                                                                                        agagTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATggg                               <  1:3495869/150‑1 (MQ=255)

                                                                                                                         gagTATTACATTGTTTTCGGGGCGTTATAGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGa                              <  2:3851423/150‑1 (MQ=255)

                                                                                                                         gagTATTACATTGTTTTCGGGGAGTTATAGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGa                              <  2:455347/150‑1 (MQ=255)

                                                                                                                         gagTATTACAGTGTTTTCGGGGCGTTATAGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGa                              <  1:3757972/150‑1 (MQ=255)

                                                                                                                             aTTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAAttt                          <  1:4478531/150‑1 (MQ=255)

                                                                                                                              ttACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAAtttt                         <  1:3021717/150‑1 (MQ=255)

                                                                                                                                aCATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTaa                       >  2:2999351/1‑150 (MQ=255)

                                                                                                                                 cATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTaaa                      <  2:1235442/150‑1 (MQ=255)

                                                                                                                                      ttttCGGGGCGTTATAGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGTGTATTTATGGGAATTTTAAAAAGtt                 <  1:3821174/150‑1 (MQ=255)

                                                                                                                                      ttttCGGGGCGTTATAGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGtt                 <  1:1516529/150‑1 (MQ=255)

                                                                                                                                         tCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGACTTTTAAAAAGtttat              >  2:3756785/1‑150 (MQ=255)

                                                                                                                                         tCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGtttat              <  1:4409677/150‑1 (MQ=255)

                                                                                                                                             ggCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTAc          <  1:2612024/150‑1 (MQ=255)

                                                                                                                                             ggCGTTATAGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTAc          <  2:3935897/150‑1 (MQ=255)

CGAAACCTTTATTTGTCCGGTGAGTAGTATCAGCAATCCAGATGAATTTAATACCTTCTTATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGA  >  CP009273/4400105‑4400403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`