Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,458,688 T→A 18.9% L416Q (CTG→CAG)  mgtA → magnesium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,458,6880TA18.9% 603.0 / 132.0 228L416Q (CTG→CAG) mgtAmagnesium transporter
Reads supporting (aligned to +/- strand):  ref base T (93/92);  new base A (20/23);  total (113/115)
Fisher's exact test for biased strand distribution p-value = 7.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 228 total aligned reads displayed.

TGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTG  >  CP009273/4458541‑4458836
                                                                                                                                                   |                                                                                                                                                    
tGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGc                                                                                                                                                    <  2:2901174/150‑1 (MQ=255)
  gATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTc                                                                                                                                                  <  1:2557475/150‑1 (MQ=255)
    tatTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGa                                                                                                                                                <  2:350171/150‑1 (MQ=255)
    tatTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGa                                                                                                                                                >  1:2521770/1‑150 (MQ=255)
           tgCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCg                                                                                                                                         <  1:4185140/150‑1 (MQ=255)
             cACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGt                                                                                                                                       >  1:3970892/1‑150 (MQ=255)
               cTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGc                                                                                                                                     >  2:2195961/1‑150 (MQ=255)
                tGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCt                                                                                                                                    <  2:3975248/150‑1 (MQ=255)
                tGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCt                                                                                                                                    >  1:819630/1‑150 (MQ=255)
                tGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCt                                                                                                                                    <  2:4358688/150‑1 (MQ=255)
                  aTAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCg                                                                                                                                  >  1:2748507/1‑150 (MQ=255)
                    aaaaCCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGaa                                                                                                                                >  2:3225474/1‑150 (MQ=255)
                     aaaCCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAg                                                                                                                               >  1:1997/1‑150 (MQ=255)
                     aaaCCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAg                                                                                                                               >  1:308744/1‑150 (MQ=255)
                     aaaCCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCAGCTCGATACAGCGGTGCTCGAAg                                                                                                                               >  2:2055688/1‑150 (MQ=255)
                     aaaCCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCAGCTCGATACAGCGGTGCTCGAAg                                                                                                                               >  2:4112017/1‑150 (MQ=255)
                        ccGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTa                                                                                                                            <  1:3720154/150‑1 (MQ=255)
                         cGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCAGCTCGATACAGCGGTGCTCGAAGGTAc                                                                                                                           <  2:2267814/150‑1 (MQ=255)
                           gCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACgg                                                                                                                         <  1:255283/150‑1 (MQ=255)
                           gCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACgg                                                                                                                         <  1:2687094/150‑1 (MQ=255)
                           gCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACgg                                                                                                                         >  1:3361333/1‑150 (MQ=255)
                              cccTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATg                                                                                                                      <  1:1169072/150‑1 (MQ=255)
                               ccTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGa                                                                                                                     >  1:3466803/1‑150 (MQ=255)
                                     gCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTc                                                                                                               <  2:4174017/150‑1 (MQ=255)
                                     gCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCAGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTc                                                                                                               <  2:4368857/150‑1 (MQ=255)
                                      cAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCa                                                                                                              <  1:413352/150‑1 (MQ=255)
                                       aGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCAGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAg                                                                                                             >  1:2136728/1‑150 (MQ=255)
                                        ggATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAgc                                                                                                            <  1:3443303/150‑1 (MQ=255)
                                        ggATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAgc                                                                                                            <  1:995483/150‑1 (MQ=255)
                                           tAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAgcgcg                                                                                                         >  2:2090814/1‑150 (MQ=255)
                                           tAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAgcgcg                                                                                                         >  2:881326/1‑150 (MQ=255)
                                           tAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAgcgcg                                                                                                         >  2:2090613/1‑150 (MQ=255)
                                           tAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAgcgcg                                                                                                         >  2:3317180/1‑150 (MQ=255)
                                             aaaTTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGcgct                                                                                                       >  2:3027344/1‑150 (MQ=255)
                                               aTTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCAGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGcgctcg                                                                                                     <  2:2914014/150‑1 (MQ=255)
                                                     cTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGcc                                                                                               >  1:3318605/1‑150 (MQ=255)
                                                     cTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGcc                                                                                               >  1:3318564/1‑150 (MQ=255)
                                                      tGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCa                                                                                              >  2:4092495/1‑150 (MQ=255)
                                                         agaATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTc                                                                                           <  1:4709946/150‑1 (MQ=255)
                                                          gaATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCAGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCg                                                                                          >  2:4216441/1‑150 (MQ=255)
                                                           aaTCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGt                                                                                         >  2:1269878/1‑150 (MQ=255)
                                                            aTCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGtt                                                                                        <  1:1469775/150‑1 (MQ=255)
                                                              cATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCAGCTCGAGACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTgg                                                                                      <  2:3157112/150‑1 (MQ=255)
                                                                tACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCa                                                                                    <  1:113761/150‑1 (MQ=255)
                                                                    gATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCAGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCaaaaa                                                                                >  2:3653201/1‑150 (MQ=255)
                                                                         ctcCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATtga                                                                           <  1:3414207/150‑1 (MQ=255)
                                                                          tcCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATtgat                                                                          <  2:712854/150‑1 (MQ=255)
                                                                             ggTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATgag                                                                       >  1:922398/1‑150 (MQ=255)
                                                                               tAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGAt                                                                     <  1:2207768/150‑1 (MQ=255)
                                                                                aaaaCCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGAtt                                                                    >  1:1707715/1‑150 (MQ=255)
                                                                                 aaaCCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTc                                                                   <  2:4357181/150‑1 (MQ=255)
                                                                                  aaCCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTcc                                                                  >  1:4110836/1‑150 (MQ=255)
                                                                                   aCCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCAGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCg                                                                 >  1:1341308/1‑150 (MQ=255)
                                                                                     cAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCACAAAATTGATGAGATTCCGtt                                                               >  1:2928030/1‑150 (MQ=255)
                                                                                     cAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGtt                                                               >  2:835777/1‑150 (MQ=255)
                                                                                     cAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCAGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGtt                                                               >  2:1523331/1‑150 (MQ=255)
                                                                                      aGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGttt                                                              <  2:4069083/150‑1 (MQ=255)
                                                                                      aGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGttt                                                              <  2:128125/150‑1 (MQ=255)
                                                                                          aaCGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGAtt                                                          >  1:4594427/1‑150 (MQ=255)
                                                                                               gTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAg                                                     >  2:1793711/1‑150 (MQ=255)
                                                                                               gTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAg                                                     <  2:1989858/150‑1 (MQ=255)
                                                                                                tgctgcATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGc                                                    >  2:480328/1‑150 (MQ=255)
                                                                                                tgctgcATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGc                                                    >  1:4064375/1‑150 (MQ=255)
                                                                                                  ctgcATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCAGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGt                                                  >  2:1304187/1‑150 (MQ=255)
                                                                                                   tgcATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTc                                                 >  1:2735682/1‑150 (MQ=255)
                                                                                                       tAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCg                                             >  2:533797/1‑150 (MQ=255)
                                                                                                        aGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCgg                                            >  1:454381/1‑150 (MQ=255)
                                                                                                            cgTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGt                                        <  2:1301905/150‑1 (MQ=255)
                                                                                                              tGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCg                                      <  2:3154984/150‑1 (MQ=255)
                                                                                                               ggTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCgg                                     >  2:2404921/1‑150 (MQ=255)
                                                                                                               ggTTGAACAGTCATTATCAGACCGGACTTAAAAACCAGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCgg                                     >  1:1643357/1‑150 (MQ=255)
                                                                                                                gTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCggt                                    >  2:1888952/1‑150 (MQ=255)
                                                                                                                gTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCggt                                    <  1:1001877/150‑1 (MQ=255)
                                                                                                                    aaCAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTa                                <  1:4728337/150‑1 (MQ=255)
                                                                                                                       aGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTg                             <  1:3031357/150‑1 (MQ=255)
                                                                                                                        gTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTgg                            <  1:596581/150‑1 (MQ=255)
                                                                                                                        gTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTgg                            <  2:3035375/150‑1 (MQ=255)
                                                                                                                         tCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGc                           >  1:4192235/1‑150 (MQ=255)
                                                                                                                          cATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCa                          <  1:899322/150‑1 (MQ=255)
                                                                                                                            ttATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGa                        <  2:1625853/150‑1 (MQ=255)
                                                                                                                              aTCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGAGTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGaaa                      <  1:2623607/150‑1 (MQ=255)
                                                                                                                                 aGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATa                   >  2:4540950/1‑150 (MQ=255)
                                                                                                                                 aGACCGGACTTAAAAACCAGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATa                   <  1:2864859/150‑1 (MQ=255)
                                                                                                                                 aGACCGGACTTAAAAACCAGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATa                   <  1:429842/150‑1 (MQ=255)
                                                                                                                                    ccGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCg                <  2:3332781/150‑1 (MQ=255)
                                                                                                                                     cGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGa               >  2:951224/1‑150 (MQ=255)
                                                                                                                                      ggACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAg              >  1:3544982/1‑150 (MQ=255)
                                                                                                                                        aCTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCa            <  1:1534281/150‑1 (MQ=255)
                                                                                                                                        aCTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCa            >  1:706653/1‑150 (MQ=255)
                                                                                                                                         cTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCAc           >  2:666886/1‑150 (MQ=255)
                                                                                                                                           tAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCa         >  2:2426850/1‑150 (MQ=255)
                                                                                                                                           tAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCa         >  1:4386706/1‑150 (MQ=255)
                                                                                                                                           tAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCa         >  2:4874847/1‑150 (MQ=255)
                                                                                                                                           tAAAAACCAGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCa         <  2:697783/150‑1 (MQ=255)
                                                                                                                                           tAAAAACCAGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCa         <  2:697878/150‑1 (MQ=255)
                                                                                                                                             aaaaCCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATc       <  1:3833298/150‑1 (MQ=255)
                                                                                                                                               aaCCAGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAg     <  2:3534902/150‑1 (MQ=255)
                                                                                                                                                aCCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGc    >  2:2746933/1‑150 (MQ=255)
                                                                                                                                                aCCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGc    <  2:1082713/150‑1 (MQ=255)
                                                                                                                                                  cTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGCGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGAGTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTg  <  2:1467716/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTG  >  CP009273/4458541‑4458836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑