BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,495,560	A→T	50.0%	N153Y (AAC→TAC)	yjhC →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,495,560	0	A	T	50.0%	‑5.6 / 260.9	146	N153Y (AAC→TAC)	yjhC	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base A (46/27); new base T (43/30); total (89/57)
Fisher's exact test for biased strand distribution p-value = 7.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 155 total aligned reads displayed.

GTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTATGATTGGTGGAAATTTGGCCCATT  >  CP009273/4495413‑4495707

   aaaGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAatacaaa                                                                                                                                                >  2:4193430/1‑150 (MQ=255)

    aaGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAatacaaac                                                                                                                                               <  2:2270271/150‑1 (MQ=255)

    aaGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAatacaaac                                                                                                                                               <  2:2270160/150‑1 (MQ=255)

    aaGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAatacaaac                                                                                                                                               <  2:1584607/150‑1 (MQ=255)

    aaGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAaaacaaac                                                                                                                                               <  2:194391/150‑1 (MQ=255)

    aaGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAaaacaaac                                                                                                                                               <  2:2621380/150‑1 (MQ=255)

      gCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAATacaaacaa                                                                                                                                             <  1:2275109/150‑1 (MQ=255)

       cGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAATACAAACAag                                                                                                                                            <  1:2528763/150‑1 (MQ=255)

        gtgtAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAATACAAACAaga                                                                                                                                           <  2:4211588/150‑1 (MQ=255)

        gtgtAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAaga                                                                                                                                           >  2:2541023/1‑150 (MQ=255)

            aaaGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAgagaga                                                                                                                                       >  1:876986/1‑150 (MQ=255)

             aagaagCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGAc                                                                                                                                      >  1:4735869/1‑150 (MQ=255)

             aagaagCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGAc                                                                                                                                      >  2:126598/1‑150 (MQ=255)

               gaagCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACtt                                                                                                                                    <  2:576507/150‑1 (MQ=255)

               gaagCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACtt                                                                                                                                    <  2:577093/150‑1 (MQ=255)

                  gCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTcc                                                                                                                                 <  1:1829390/150‑1 (MQ=255)

                    tGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTCCTg                                                                                                                               >  2:790021/1‑150 (MQ=255)

                     ggTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTCCTgg                                                                                                                              >  1:1702685/1‑150 (MQ=255)

                                tATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGaa                                                                                                                   >  2:2020293/1‑150 (MQ=255)

                                 aTGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACGTTCCTGGAAAAAGATGaaa                                                                                                                  <  2:2873446/150‑1 (MQ=255)

                                  tGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAg                                                                                                                 >  2:2847722/1‑150 (MQ=255)

                                  tGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAg                                                                                                                 >  2:773454/1‑150 (MQ=255)

                                   ggCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGa                                                                                                                <  1:3711056/150‑1 (MQ=255)

                                          cATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCt                                                                                                         >  1:4089445/1‑150 (MQ=255)

                                           atatTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCtg                                                                                                        >  2:4593607/1‑150 (MQ=255)

                                             attatGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCtggt                                                                                                      >  1:3690364/1‑150 (MQ=255)

                                                      tttttCAATGGGGTTCAATATGCACGGTAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCta                                                                                             <  1:928267/150‑1 (MQ=255)

                                                      tttttCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCta                                                                                             <  1:928432/150‑1 (MQ=255)

                                                        tttCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCtata                                                                                           >  2:2847807/1‑150 (MQ=255)

                                                         ttCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTatat                                                                                          <  1:3216099/150‑1 (MQ=255)

                                                         ttCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTatat                                                                                          >  1:3810482/1‑150 (MQ=255)

                                                          tCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATatc                                                                                         <  1:2541023/150‑1 (MQ=255)

                                                            aaTGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATatcat                                                                                       >  1:3670245/1‑150 (MQ=255)

                                                            aaTGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATatcat                                                                                       <  2:161250/150‑1 (MQ=255)

                                                             aTGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATatcatc                                                                                      <  2:3648212/150‑1 (MQ=255)

                                                               ggggTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATcatcat                                                                                    <  1:3333488/150‑1 (MQ=255)

                                                               ggggTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATcatcat                                                                                    <  1:425236/150‑1 (MQ=255)

                                                               ggggTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATcatcat                                                                                    >  1:664606/1‑150 (MQ=255)

                                                               ggggTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATcatcat                                                                                    >  1:664626/1‑150 (MQ=255)

                                                                gggTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCata                                                                                   >  1:4754914/1‑150 (MQ=255)

                                                                gggTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCata                                                                                   >  1:159710/1‑150 (MQ=255)

                                                                    tCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACa                                                                               >  2:1442220/1‑150 (MQ=255)

                                                                      aaTATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATg                                                                             <  2:4858081/150‑1 (MQ=255)

                                                                      aaTATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATg                                                                             <  2:4858116/150‑1 (MQ=255)

                                                                       atatGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGa                                                                            >  2:3370505/1‑150 (MQ=255)

                                                                       atatGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGa                                                                            >  2:3373371/1‑150 (MQ=255)

                                                                       atatGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGa                                                                            >  1:3479865/1‑150 (MQ=255)

                                                                       atatGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGa                                                                            <  2:184996/150‑1 (MQ=255)

                                                                        tatGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAg                                                                           <  1:1032042/150‑1 (MQ=255)

                                                                          tGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGtt                                                                         >  2:276360/1‑150 (MQ=255)

                                                                            cACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATAATCATATACATGAGTTAg                                                                       >  1:518077/1‑150 (MQ=255)

                                                                            cACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAg                                                                       <  1:3099465/150‑1 (MQ=255)

                                                                                gAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATtg                                                                   >  2:4447863/1‑150 (MQ=255)

                                                                                  aGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATtgtg                                                                 >  2:298756/1‑150 (MQ=255)

                                                                                      aattaaAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCa                                                             >  2:179227/1‑150 (MQ=255)

                                                                                        ttaaAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGc                                                           <  1:3508433/150‑1 (MQ=255)

                                                                                        ttaaAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGc                                                           <  1:1534314/150‑1 (MQ=255)

                                                                                        ttaaAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGc                                                           <  1:1533048/150‑1 (MQ=255)

                                                                                        ttaaAGAAGGTGTTATCGGCGAAATAATATCATGTCATACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTTTGTTCAGc                                                           >  1:3260289/1‑150 (MQ=255)

                                                                                            agaagGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCAttt                                                       <  2:3850327/150‑1 (MQ=255)

                                                                                             gaagGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTa                                                      <  2:4189946/150‑1 (MQ=255)

                                                                                              aagGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTAc                                                     <  2:922064/150‑1 (MQ=255)

                                                                                                  tgtTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTgg                                                 >  2:312762/1‑150 (MQ=255)

                                                                                                    ttATCGGCTAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGgag                                               <  2:4589641/150‑1 (MQ=255)

                                                                                                    ttATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGTAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGgag                                               >  2:3477519/1‑150 (MQ=255)

                                                                                                            gAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCa                                       >  1:4131804/1‑150 (MQ=255)

                                                                                                            gAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCa                                       >  2:2724379/1‑150 (MQ=255)

                                                                                                                tattatCATGTCATACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAgaga                                   >  1:1115339/1‑150 (MQ=255)

                                                                                                                  ttatCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACg                                 >  2:3749994/1‑150 (MQ=255)

                                                                                                                       aTGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTAc                            >  1:317657/1‑150 (MQ=255)

                                                                                                                        tGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACt                           >  1:3874314/1‑150 (MQ=255)

                                                                                                                        tGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACt                           >  1:2932805/1‑150 (MQ=255)

                                                                                                                        tGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACt                           >  1:4152641/1‑150 (MQ=255)

                                                                                                                        tGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACt                           <  2:1129687/150‑1 (MQ=255)

                                                                                                                         gTCATACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTa                          <  1:4533986/150‑1 (MQ=255)

                                                                                                                         gTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTa                          >  2:42117/1‑150 (MQ=255)

                                                                                                                          tCATACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTAt                         >  2:1274300/1‑150 (MQ=255)

                                                                                                                           cATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGTTTACTATg                        >  2:100357/1‑150 (MQ=255)

                                                                                                                            aTACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTATGa                       >  1:4129896/1‑150 (MQ=255)

                                                                                                                            aTACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTATGa                       >  1:2102206/1‑150 (MQ=255)

                                                                                                                            aTACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTATGa                       >  1:1201081/1‑150 (MQ=255)

                                                                                                                            aTACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTATGa                       >  1:195217/1‑150 (MQ=255)

                                                                                                                             tACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTATGAt                      >  2:3182078/1‑150 (MQ=255)

                                                                                                                             tACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTCCTATGAt                      >  1:4800020/1‑150 (MQ=255)

                                                                                                                               cTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTATGATtg                    >  2:3112771/1‑150 (MQ=255)

                                                                                                                               cTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTATGATtg                    >  1:3799451/1‑150 (MQ=255)

                                                                                                                                   gagaAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTATGATtggtgg                >  2:1917913/1‑150 (MQ=255)

                                                                                                                                   gagaAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTATGATtggtgg                <  1:2965364/150‑1 (MQ=255)

                                                                                                                                   gagaAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTATGATtggtgg                >  1:4144061/1‑150 (MQ=255)

                                                                                                                                    agaAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTATGATTGGTGGa               >  2:1139154/1‑150 (MQ=255)

                                                                                                                                    agaAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTATGATTGGTGGa               >  2:1687100/1‑150 (MQ=255)

                                                                                                                                    agaAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTATGATTGGTGGa               >  1:1391922/1‑150 (MQ=255)

                                                                                                                                      aaaTGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTATGATTGGTGGaaa             >  1:3243334/1‑150 (MQ=255)

                                                                                                                                        aTGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTAAATCATCATATACATGAGTTAGTTTGTGTTCAGCATATACTTGGAGAAATACCAGAGACGGTTACTATGATTGGTGGAAAtt           >  2:1010495/1‑150 (MQ=255)

                                                                                                                                        aTGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTATGATTGGTGGAAAtt           <  1:2360867/150‑1 (MQ=255)

                                                                                                                                        aTGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTATGATTGGTGGAAAtt           >  1:4572259/1‑150 (MQ=255)

                                                                                                                                         tGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTATGATTGGTGGAAAttt          >  1:2016095/1‑150 (MQ=255)

                                                                                                                                             tGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTATGATTGGTGGAAATTTGGcc      <  1:1290300/150‑1 (MQ=255)

                                                                                                                                              gggAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTATGATTGGTGGAAATTTGGccc     >  2:290226/1‑150 (MQ=255)

                                                                                                                                                 aaaaCAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTATGATTGGTGGAAATTTGGCCCAtt  >  1:3407701/1‑150 (MQ=255)

GTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTATGATTGGTGGAAATTTGGCCCATT  >  CP009273/4495413‑4495707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`