Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,524,046 C→A 27.5% intergenic (‑176/+201) yjhX ← / ← yjhZ UPF0386 family protein/pseudogene, rimK paralog, C‑terminal fragment

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,524,0460CA27.5% 423.2 / 162.0 203intergenic (‑176/+201)yjhX/yjhZUPF0386 family protein/pseudogene, rimK paralog, C‑terminal fragment
Reads supporting (aligned to +/- strand):  ref base C (70/77);  new base A (30/26);  total (100/103)
Fisher's exact test for biased strand distribution p-value = 5.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01

Only 100 of 206 total aligned reads displayed.

TGCAAAACGTGCAAACACACCGCGGTGTCCGCATTCGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAAATTGAATGCCAACCCATTTACTGGAAAAGGCAGATGACACTGACGAATGCATGTTGAAGTACGCCTGAAATGGCCTCATCGGC  >  CP009273/4523897‑4524193
                                                                                                                                                     |                                                                                                                                                   
tgCAAAACGTGCAAACACACCGCGGTGTCCGCATTCGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGc                                                                                                                                                     >  2:163374/1‑150 (MQ=255)
tgCAAAACGTGCAAACACACCGCGGTGTCCGCATTCGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGc                                                                                                                                                     <  1:127500/150‑1 (MQ=255)
 gCAAAACGTGCAAACACACCGCGGTGTCCGCATTCGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCg                                                                                                                                                    <  1:4751197/150‑1 (MQ=255)
  cAAAACGTGCAAACACACCGCGGTGTCCGCATTCGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGa                                                                                                                                                   <  2:1698540/150‑1 (MQ=255)
  cAAAACGTGCAAACACACCGCGGTGTCCGCATTCGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGa                                                                                                                                                   >  2:4095205/1‑150 (MQ=255)
   aaaaCGTGCAAACACACCGCGGTGTCCGCATTCGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGaa                                                                                                                                                  >  1:872527/1‑150 (MQ=255)
   aaaaCGTGCAAACACACCGCGGTGTCCGCATTCGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGaa                                                                                                                                                  >  1:4368884/1‑150 (MQ=255)
   aaaaCGTGCAAACACACCGCGGTGTCCGCATTCGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGaa                                                                                                                                                  >  1:4369185/1‑150 (MQ=255)
   aaaaCGTGCAAACACACCGCGGTGTCCGCATTCGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGaa                                                                                                                                                  <  1:1230718/150‑1 (MQ=255)
    aaaCGTGCAAACACACCGCGGTGTCCGCATTCGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAat                                                                                                                                                 <  2:54742/150‑1 (MQ=255)
     aaCGTGCAAACACACCGCGGTGTCCGCATTCGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTCTTTACGGAGCGActa                                                                                                                                                >  2:279697/1‑150 (MQ=255)
     aaCGTGCAAACACACCGCGGTGTCCGCATTCGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAata                                                                                                                                                <  1:1330641/150‑1 (MQ=255)
     aaCGTGCAAACACACCGCGGTGTCCGCATTCGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAata                                                                                                                                                <  1:1142729/150‑1 (MQ=255)
      aCGTGCAAACACACCGCGGTGTCCGCATTCGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAatat                                                                                                                                               <  1:432805/150‑1 (MQ=255)
        gTGCAAACACACCGCGGTGTCCGCATTCGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGAGAATATTa                                                                                                                                             >  2:677297/1‑150 (MQ=255)
         tGCAAACACACCGCGGTGTCCGCATTCGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGAGAATATTaa                                                                                                                                            <  1:618368/150‑1 (MQ=255)
          gCAAACACACCGCGGTGTCCGCATTCGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAac                                                                                                                                           >  1:4671508/1‑150 (MQ=255)
          gCAAACACACCGCGGTGTCCGCATTCGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAac                                                                                                                                           <  2:602362/150‑1 (MQ=255)
          gCAAACACACCGCGGTGTCCGCATTCGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGAGAATATTAac                                                                                                                                           <  2:4780379/150‑1 (MQ=255)
           cAAACACACCGCGGTGTCCGCATTCGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGAGAATATTAaca                                                                                                                                          >  1:112181/1‑150 (MQ=255)
            aaaCACACCGCGGTGTCCGCATTCGATTTCGGCGCATTGATAATCAGTCCGTCCTGAAAATGTCGGGGAACTGATTATCGGTTGATTTCATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGATAGGTATTTACGGAGCGAATATTAaaac                                                                                                                                         <  1:2071368/150‑1 (MQ=255)
            aaaCACACCGCGGTGTCCGCATTCGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAacac                                                                                                                                         >  1:2862063/1‑150 (MQ=255)
            aaaCACACCGCGGTGTCCGCATTCGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAacac                                                                                                                                         <  1:403565/150‑1 (MQ=255)
             aaCACACCGCGGTGTCCGCATTCGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACg                                                                                                                                        <  2:2049357/150‑1 (MQ=255)
             aaCACACCGCGGTGTCCGCATTCGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACg                                                                                                                                        >  2:2273903/1‑150 (MQ=255)
             aaCACACCGCGGTGTCCGCATTCGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGAGAATATTAACACg                                                                                                                                        >  2:3735117/1‑150 (MQ=255)
              acacacCGCGGTGTCCGCATTCGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGAGAATATTAACACGt                                                                                                                                       >  2:1718538/1‑150 (MQ=255)
                  acCGCGGGGTCCGCATTCGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGtt                                                                                                                                   <  2:4530079/150‑1 (MQ=255)
                   ccGCGGTGTCCGCATTCGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGAGAATATTAACACGTAGTta                                                                                                                                  <  2:3489866/150‑1 (MQ=255)
                   ccGCGGTGTCCGCATTCGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGAGAATATTAACACGTAGTta                                                                                                                                  <  2:1035140/150‑1 (MQ=255)
                    cgcgGTGTCCGCATTCGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTtat                                                                                                                                 <  1:4252625/150‑1 (MQ=255)
                     gcgGTGTCCGCATTCGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTtata                                                                                                                                <  1:4290754/150‑1 (MQ=255)
                         tgtCCGCATTCGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATaggag                                                                                                                            >  1:611563/1‑150 (MQ=255)
                                aTTCGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATg                                                                                                                     >  1:4229597/1‑150 (MQ=255)
                                aTTCGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATg                                                                                                                     >  2:3069110/1‑150 (MQ=255)
                                 ttCGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGt                                                                                                                    <  2:822914/150‑1 (MQ=255)
                                 ttCGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGt                                                                                                                    <  2:824342/150‑1 (MQ=255)
                                  tCGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGtt                                                                                                                   >  1:3205273/1‑150 (MQ=255)
                                  tCGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGAGAATATTAACACGTAGTTATAGGAGGGATATGtt                                                                                                                   <  1:4007518/150‑1 (MQ=255)
                                   cGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGttt                                                                                                                  <  2:1545148/150‑1 (MQ=255)
                                     aTTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCa                                                                                                                <  2:3437704/150‑1 (MQ=255)
                                       ttCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGt                                                                                                              <  1:1048607/150‑1 (MQ=255)
                                         cGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAg                                                                                                            <  1:3769772/150‑1 (MQ=255)
                                               aTTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAg                                                                                                      >  2:4053156/1‑150 (MQ=255)
                                                ttGATAATCAGTCCGGCCTTAAAATGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGAGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAgt                                                                                                     <  2:4125610/150‑1 (MQ=255)
                                                 tGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGAGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAgtg                                                                                                    >  2:4297374/1‑150 (MQ=255)
                                                   aTAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGtgtg                                                                                                  <  2:1572819/150‑1 (MQ=255)
                                                         aGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTAc                                                                                            >  2:3810066/1‑150 (MQ=255)
                                                         aGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGAGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTAc                                                                                            >  2:3982629/1‑150 (MQ=255)
                                                         aGTCCGGCCTGAAAAGGTCGGGGAACTGAGTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGGTGAGTTGAAAGGTATGTACGGAGAGAATATTAACACGTAGTTAGAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTAc                                                                                            >  2:2464772/1‑150 (MQ=255)
                                                          gTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACt                                                                                           <  2:459767/150‑1 (MQ=255)
                                                            ccGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACttt                                                                                         >  1:1580230/1‑150 (MQ=255)
                                                             cGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTg                                                                                        >  2:3291510/1‑150 (MQ=255)
                                                             cGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTg                                                                                        <  2:2206064/150‑1 (MQ=255)
                                                              ggCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTgg                                                                                       >  1:1214645/1‑150 (MQ=255)
                                                               gCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGa                                                                                      <  1:352433/150‑1 (MQ=255)
                                                                ccTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGaa                                                                                     >  2:1601270/1‑150 (MQ=255)
                                                                 cTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGAGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGaat                                                                                    <  2:3118350/150‑2 (MQ=255)
                                                                   gAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAAAtt                                                                                  <  1:4859942/150‑1 (MQ=255)
                                                                              ggAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAAATTGAATGCCAAcc                                                                       >  2:3997268/1‑150 (MQ=255)
                                                                               gAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAAATTGAATGCCAAcca                                                                      <  2:1954835/150‑2 (MQ=255)
                                                                                aaCTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAAATTGAATGCCAACCCa                                                                     <  2:1311134/150‑1 (MQ=255)
                                                                                 aCTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGAGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAATTTGAATGCCAACCCAt                                                                    <  2:4226125/150‑1 (MQ=255)
                                                                                   tGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAAATTGAATGCCAACCCAttt                                                                  >  1:20017/1‑150 (MQ=255)
                                                                                   tGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAAATTGAATGCCAACCCAttt                                                                  >  2:3362573/1‑150 (MQ=255)
                                                                                    gATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAAATTGAATGCCAACCCATTTa                                                                 <  2:4671508/150‑1 (MQ=255)
                                                                                     aTTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAAATTGAATGCCAACCCATTTAc                                                                >  2:2559388/1‑150 (MQ=255)
                                                                                      ttATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAAATTGAATGCCAACCCATTTACt                                                               <  1:4192514/150‑1 (MQ=255)
                                                                                      ttATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGAGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAATTTGAATGCCAACCCATTTACt                                                               <  2:4426942/150‑1 (MQ=255)
                                                                                          cGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGAGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAATTTGAATGCCAACCCATTTACTGGaa                                                           <  1:4624580/150‑1 (MQ=255)
                                                                                             atgatTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAAATTGAATGCCAACCCATTTACTGGAAAAg                                                        <  2:4305578/150‑1 (MQ=255)
                                                                                                atTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAAATTGAATGCCAACCCATTTACTGGAAAAGGCa                                                     >  2:3331508/1‑150 (MQ=255)
                                                                                                atTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAAATTGAATGCCAACCCATTTACTGGAAAAGGCa                                                     >  2:2631532/1‑150 (MQ=255)
                                                                                                  tACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAAATTGAATGCCAACCCATTTACTGGAAAAGGCAGa                                                   >  2:683446/1‑150 (MQ=255)
                                                                                                  tACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGAGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAATTTGAATGCCAACCCATTTACTGGAAAAGGCAGa                                                   >  1:1075610/1‑150 (MQ=255)
                                                                                                      ttCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAAATTGAATGCCAACCCATTTACTGGAAAAGGCAGATGac                                               >  2:2515344/1‑150 (MQ=255)
                                                                                                       tctcCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAAATTGAATGCCAACCCATTTACTGGAAAAGGCAGATGaca                                              <  1:1730110/150‑1 (MQ=255)
                                                                                                            aGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAAATTGAATGCCAACCCATTTACTGGAAAAGGCAGATGACACTGAc                                         <  2:3491961/150‑1 (MQ=255)
                                                                                                               aTCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAAATTGAATGCCAACCCATTTACTGGAAAAGGCAGATGACACTGACGaa                                      <  2:1491981/150‑1 (MQ=255)
                                                                                                               aTCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGAGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAATTTGAATGCCAACCCATTTACTGGAAAAGGCAGATGACACTGACGaa                                      >  1:2145004/1‑150 (MQ=255)
                                                                                                               aTCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGAGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAATTTGAATGCCAACCCATTTACTGGAAAAGGCAGATGACACTGACGaa                                      >  1:4232673/1‑150 (MQ=255)
                                                                                                                tCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGAGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAATTTGAATGCCAACCCATTTACTGGAAAAGGCAGATGACACTGACGAAt                                     <  1:2589306/150‑1 (MQ=255)
                                                                                                                 cAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAAATTGAATGCCAACCCATTTACTGGAAAAGGCAGATGACACTGACGAATg                                    >  2:475067/1‑150 (MQ=255)
                                                                                                                   aaGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGAGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAATTTGAATGCCAACCCATTTACTGGAAAAGGCAGATGACACTGACGAATGCa                                  >  2:2023172/1‑150 (MQ=255)
                                                                                                                      ccTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAAATTGAATGCCAACCCATTTACTGGAAAAGGCAGATGACACTGACGAATGCATGt                               <  1:4784888/150‑1 (MQ=255)
                                                                                                                       cTCGGGTTGAGTTGAAAGGTATTTACGGAGAGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAATTTGAATGCCAACCCATTTACTGGAAAAGGCAGATGACACTGACGAATGCATGtt                              >  1:2903889/1‑150 (MQ=255)
                                                                                                                        tCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAAATTGAATGCCAACCCATTTACTGGAAAAGGCAGATGACACTGACGAATGCATGTTg                             >  1:4628100/1‑150 (MQ=255)
                                                                                                                         cGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAAATTGAATGCCAACCCATTTACTGGAAAAGGCAGATGACACTGACGAATGCATGTTGa                            <  2:843725/150‑1 (MQ=255)
                                                                                                                          gggTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAAATTGAATGCCAACCCATTTACTGGAAAAGGCAGATGACACTGACGAATGCATGTTGaa                           <  1:2580254/150‑1 (MQ=255)
                                                                                                                          gggTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAAATTGAATGCCAACCCATTTACTGGAAAAGGCAGATGACACTGACGAATGCATGTTGaa                           >  1:734385/1‑150 (MQ=255)
                                                                                                                          gggTTGAGTTGAAAGGTATTTACGGAGAGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAATTTGAATGCCAACCCATTTACTGGAAAAGGCAGATGACACTGACGAATGCATGTTGaa                           >  1:3164201/1‑150 (MQ=255)
                                                                                                                           ggTTGAGTTGAAAGGTATTTACGGAGAGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAATTTGAATGCCAACCCATTTACTGGAAAAGGCAGATGACACTGACGAATGCATGTTGAAg                          >  2:3625588/1‑150 (MQ=255)
                                                                                                                                agttgaAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAAATTGAATGCCAACCCATTTACTGGAAAAGGCAGATGACACTGACGAATGCATGTTGAAGTACGc                     >  1:2997477/1‑150 (MQ=255)
                                                                                                                                agttgaAAGGTATTTACGGAGAGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAATTTGAATGCCAACCCATTTACTGGAAAAGGCAGATGACACTGACGAATGCATGTTGAAGTACGc                     >  1:229364/1‑150 (MQ=255)
                                                                                                                                         gTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAAATTGAATGCCAACCCATTTACTGGAAAAGGCAGATGACACTGACGAATGCATGTTGAAGTACGCCTGAAATgg            <  1:279697/150‑1 (MQ=255)
                                                                                                                                          tATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAAATTGAATGCCAACCCATTTACTGGAAAAGGCAGATGACACTGACGAATGCATGTTGAAGTACGCCTGAAATGGc           <  2:4147650/150‑1 (MQ=255)
                                                                                                                                          tATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAAATTGAATGCCAACCCATTTACTGGAAAAGGCAGATGACACTGACGAATGCATGTTGAAGTACGCCTGAAATGGc           >  2:1882926/1‑150 (MQ=255)
                                                                                                                                           aTTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAAATTGAATGCCAACCCATTTACTGGAAAAGGCAGATGACACTGACGAATGCATGTTGAAGTACGCCTGAAATGGcc          >  1:3891790/1‑150 (MQ=255)
                                                                                                                                              tACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAAATTGAATGCCAACCCATTTACTGGAAAAGGCAGATGACACTGACGAATGCATGTTGAAGTACGCCTGAAATGGCCTCa       >  1:3347077/1‑150 (MQ=255)
                                                                                                                                              tACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAAATTGAATGCCAACCCATTTACTGGAAAAGGCAGATGACACTGACGAATGCATGTTGAAGTACGCCTGAAATGGCCTCa       <  1:4053156/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TGCAAAACGTGCAAACACACCGCGGTGTCCGCATTCGATTTCGGCGCATTGATAATCAGTCCGGCCTGAAAAGGTCGGGGAACTGATTATCGGATGATTACATTCTCCAGCATCAAAGCCTCGGGTTGAGTTGAAAGGTATTTACGGAGCGAATATTAACACGTAGTTATAGGAGGGATATGTTTCAGTAGAAAAAGTGTGATGTACTTTGGAAATTGAATGCCAACCCATTTACTGGAAAAGGCAGATGACACTGACGAATGCATGTTGAAGTACGCCTGAAATGGCCTCATCGGC  >  CP009273/4523897‑4524193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑