| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 360,114 | A→T | 6.4% | intergenic (‑48/+547) | lacZ ← / ← lacI | pseudogene, truncated/DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 360,114 | 0 | A | T | 6.4% | 1137.6 / 10.2 | 385 | intergenic (‑48/+547) | lacZ/lacI | pseudogene, truncated/DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base A (159/191); new base T (5/29); total (164/221) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.33e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 389 total aligned reads displayed.
AGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAG > CP009273/359965‑360256 | cgttgcttcgcaacgttcaaatccgctcccggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgacaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGtt > 2:3012042‑M1/137‑150 (MQ=255) cAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGtt > 2:1552241/1‑150 (MQ=255) gttgcttcgcaacgttcaaatccgctcccggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGttt > 1:3351608‑M1/136‑150 (MQ=255) aGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGttt < 2:1164312/150‑1 (MQ=255) tcaatcaaCTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATg < 2:130442/150‑1 (MQ=255) gcttcgcaacgttcaaatccgctcccggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagccgttcgttttatttgatggctggggtattAATTACTGGCAGTTTATg < 1:432781‑M1/18‑1 (MQ=255) caatcaaCTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATgg > 1:3721728/1‑150 (MQ=255) tcgcaacggtcaaatccgctcccggcggatttgtcctactcaggagagcgttcaccgacaaacaacagatacaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATggcg > 2:4482159‑M1/130‑150 (MQ=255) atcaaCTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATggcg < 2:1477592/150‑1 (MQ=255) aCTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATggcgggcg > 1:1233568/1‑150 (MQ=255) gTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATACGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTcc > 2:2890643/1‑150 (MQ=255) tttACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCt < 2:4550117/150‑1 (MQ=255) actccgctcccggcggatttgtcctactccggagagcggtcaccgacacccaccagctcacacgaccggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGccc > 1:2927017‑M1/118‑150 (MQ=255) cTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCg > 2:542312/1‑150 (MQ=255) atccgctcccggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTc > 1:2806757‑M1/117‑143 (MQ=255) cgctcccggcggatttgtcctcctcaggagaccgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcatggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCa > 1:4875986‑M1/114‑150 (MQ=255) cgctcccggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGccc > 2:4065202‑M1/114‑146 (MQ=255) gctcccggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCt < 2:4621608‑M1/29‑1 (MQ=255) ggAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCAcc > 2:2164209/1‑150 (MQ=255) gAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCAccc > 1:2688285/1‑150 (MQ=255) cccggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCt > 1:1128408‑M1/110‑150 (MQ=255) cgggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtatttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTc > 1:3466447‑M1/109‑150 (MQ=255) ccggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTc > 2:2930995‑M1/109‑150 (MQ=255) gACATCCAGAGGCACTTCACCGCTTGCAAGCGGCTTACAATCCAGCGCCACAATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCg > 1:4778547/1‑150 (MQ=255) cggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATg < 1:3551175‑M1/18‑1 (MQ=255) cATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCCGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCggg > 2:2436062/1‑150 (MQ=255) ggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCGCCGGGc < 1:4221109‑M1/47‑1 (MQ=255) tCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGcc > 2:3852315/1‑150 (MQ=255) ccAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTTAAACATGAGAATTACGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCg > 2:1792843/1‑150 (MQ=255) ccAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCg > 1:3047389/1‑150 (MQ=255) ttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGtt < 2:2294506‑M1/51‑1 (MQ=255) agagGCACTTCACCGCTTGCCAGCGGCTTACCATCCCGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGtt < 2:3242198/150‑1 (MQ=255) tgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTg < 1:3141248‑M1/52‑1 (MQ=255) ggCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCt < 2:3607038/150‑1 (MQ=255) ggCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCt > 1:810683/1‑150 (MQ=255) aCTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTGGCTTCg > 2:278836/1‑150 (MQ=255) tcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGAAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCaa < 2:1127441‑M1/60‑1 (MQ=255) cACCGCTTGCCAGCGGCTTACCAGCCCCCCCCACCATCCCGTGCTGGAGCTCCGATTATACGCTGTCAAACATGATAATTCCGATCCCCAATTCCGGGCTTTTTAGGGGGGGCGTCCGGCCCGCCACCCTCCGGGCCGTTGCTTCGCAAc < 1:3938162/150‑1 (MQ=25) aCCGCTTGCCAGCGGATTACCACCCCGCGCCACAAGCCAGTGCAGGAGCTCCTATTATATGCTGAAAAACATGATATTTCCGATCCCAAATTCCTGGCTTTTTATGGCGGGGGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACg < 1:2722271/150‑1 (MQ=21) cGCTTGCCAGCGGATTACCATCCCGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAACCATGAGAATACCGATCCCCAATTCGTGGCAGTTTATGGGGGGCGTCCGGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGtt < 1:1105340/150‑1 (MQ=37) agagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGGATCGCAACgtta < 2:3249770‑M1/65‑2 (MQ=255) gagcgttcaccgacaaccaacataaaaaactaaaggcccagtctttcgcctgagcctttcgttttattttatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCa > 2:1425771‑M1/85‑150 (MQ=255) gttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATc < 2:2768109‑M1/70‑1 (MQ=255) accgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCt > 2:3915599‑M1/77‑150 (MQ=255) ccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgccgggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGcc > 2:2807305‑M1/76‑123 (MQ=255) cgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCCCCCGCCGGGCCGGTGGGTCGCCACCATCAAATACGCTcc < 1:2589827‑M1/76‑1 (MQ=255) caaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCcgg < 1:3243439‑M1/79‑1 (MQ=255) cAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGAttt < 2:3195400/150‑1 (MQ=255) cAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGACAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGAttt < 1:2425648/150‑1 (MQ=255) cAGCGCCACCATCCAGAGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGAttt < 2:4726415/150‑1 (MQ=255) gcgcCACCATCCAGTCCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGt < 2:3365072/150‑1 (MQ=255) caccaTCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCGTGGCCAAGTTAAAATCCGCGCCCGGCGGGTTTGTccaa > 1:1424823/1‑148 (MQ=25) accaTCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACCTTAAAATAAGCTACCCGCGGATTTGtactac > 2:591431/1‑150 (MQ=25) ccaTCCTGTGCTGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCTGTTTTTGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACt < 1:4751483/150‑1 (MQ=255) ccaTCCAGCGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCCATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACt < 2:4263878/150‑1 (MQ=255) aTCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCTATCCCCAATTCCGGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCa < 1:4413347/150‑1 (MQ=255) tCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGt < 1:1975431/79‑1 (MQ=255) tCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATGCGTGGCTGTTTTTGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAg < 2:16174/150‑1 (MQ=255) aaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGccc < 1:1937616‑M1/33‑1 (MQ=255) ggcgccgtctttcgactgagcctttggttttattgtctgcgcggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGgag > 2:3721728‑M1/51‑150 (MQ=255) tGCTGGTGCGCCGTTGATAGGCTTTCAAAAATTATATTCCCGAACCCCAATTCGTGGCTGTTTATGGCGGGGGGCCGGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGagag < 1:4144299/150‑1 (MQ=14) cccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGagag > 2:1945431‑M1/49‑150 (MQ=255) ccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGGTTCGCAACGGTCAACACCCCACCCCGCGGCTTTGTGCTACACAGGAGAGc < 1:1672364‑M1/103‑1 (MQ=255) gAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAAGTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTc < 2:4311231/150‑1 (MQ=255) tttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCAc < 2:805187‑M1/109‑1 (MQ=255) tttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCGTCGCAACGTTCAAATACGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCAc < 2:1884422‑M1/109‑1 (MQ=255) gCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAAGTCCTGGCAGTTTTTGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCAc < 1:1559056/150‑1 (MQ=255) ctcgattgcgcttgtcgatttatttgaggcctgggtaattAATTCCGGGCTGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCAcc > 1:2977297‑M1/41‑150 (MQ=255) gatgatAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCGCGGCGGATTTGTCCTACTCAGGAGAGCGTGCACCGaca > 2:4875986/1‑150 (MQ=255) gatgatAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGaca > 2:2694115/1‑150 (MQ=255) agcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGacaaaca < 2:4353218‑M1/118‑1 (MQ=255) gcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGacaaacaa < 2:2594431‑M1/119‑1 (MQ=255) agcttaaaaaCATTCGATTTCCTATCCCCATTTCCTGGCTGTTTAGGGGGGGCGTCCTGCCCCCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACGAacaaca < 1:915556/143‑1 (MQ=11) tGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTTTGGCGGGCGTCCGGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGAt < 1:1466350/150‑1 (MQ=255) tGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTTCTTCGCAACGGGAAAATCCGCACCCGGCGGATTTGTCCGACAAAGGCGAGCGTTGACCGACAAAAAAAAGAt > 1:753565/1‑150 (MQ=17) cgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCAGCCACCCTCCGGGCCGTTGCTTCGCAACGTTAAAATCCGCTACCGGCGGATTTGTCAAACTAAGGAGAGCGTTCACCGACAAACAACAGATa < 2:3502475‑M1/125‑1 (MQ=255) gttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATaa > 2:2728098‑M1/25‑150 (MQ=255) gttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATaa < 2:4777758‑M1/126‑1 (MQ=255) aaaCATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATaaaa < 2:4241469/150‑1 (MQ=255) aaCATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAAc < 2:3061135/150‑1 (MQ=255) ccATGTGAATCCCGACCCCCATTTCGTGGCTTTTTTGGGGGGGGGTCCGGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACg < 1:56437/149‑1 (MQ=11) acttGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACg < 2:3300546/147‑1 (MQ=255) cATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTGGCGTGCCAAAGGTCAAATACGCACCCCGCGGATTTTTGCTAATGAGGAGATCGTTGACCGAAAAAAAACAGAAAAAAAGa > 1:1024547/1‑150 (MQ=17) aTGAGAATTCCGATCCCCAATTCCTGGCTGTTTTGGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGaa < 1:2348150/150‑1 (MQ=255) aaTTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGcc > 2:3951504/1‑150 (MQ=255) ttCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCa < 1:2778931/150‑1 (MQ=255) cGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTc < 2:3139851/150‑1 (MQ=39) aTCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGGGTCGCAAAGTTAAAAAACGCTCCCGGCGGCTTTGTGCTAAACAGGAGAGCGGTACCCCACAAACAACAGATAAAAAGAAAGGCCCAGTCtt > 2:1231585/1‑150 (MQ=11) aTCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCtt < 1:2164209/150‑1 (MQ=38) tCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTTCATCGCAAACAGAAAAACCGCTACCCGCGGATTTTTCCTAAACAGGGGAGCGTTCCCCGACAAACAAAACATAAAACGAAAGGCCCACTCatt > 1:1540843/1‑150 (MQ=11) gaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGCTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCttt < 2:3530962‑M1/145‑1 (MQ=255) atttAATTCCTGGCTGTTTCGGGCGGGCGTCCGGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTc > 2:3808396‑M1/5‑150 (MQ=255) cccATTTCCTGGGTGTTTTGGGGGGGGGGCCGGCCCGCCACCCCCCGGGGCGTTGCTTCGGAAAGGTCAAATCCGCTCCCGGCGGTTTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCg < 1:4552647/150‑1 (MQ=11) tacttCCTGGCTGTTTATGGCGGGCGTCCTGCCCGCGACCCTCCGGGCCGTTGCGTCGCTAGGTGCAAATCCGCTGCCGGCGGTTTTGTCCTACTCTGGAGAGCGTTCACGGACAACCAACAGATAAAACGAAAGGCCCAGTCTTTCGAc < 2:253501/147‑1 (MQ=11) cAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGAc < 1:1874143/150‑1 (MQ=33) cAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGAc < 2:1866234/150‑1 (MQ=33) aaTTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACt < 2:943467/150‑1 (MQ=32) aaTTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCGTGGCAACGTTCAAAAACGCTCCCGGCGGATTTGTCCGACTCAGGAGCGCGGTCACCCACCAACAACAGATAAAAAGAAAGGCCCAGTCTTTCGACt > 2:1273096/1‑150 (MQ=11) aTTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAAc < 1:402078/60‑1 (MQ=35) ttCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTGGGGTCGCAAAGGTCAAAAACGCTCCCGGGGGGTTTGTGCGGAACAGGCGCGCGGTCACCCCCCAACAACCGAAAAAAAGAAAGGCCCCCTCTTTGGACTgg > 2:2018372/1‑149 (MQ=2) | AGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAG > CP009273/359965‑360256 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |