| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 360,157 | T→G | 8.1% | intergenic (‑91/+504) | lacZ ← / ← lacI | pseudogene, truncated/DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 360,157 | 0 | T | G | 8.1% | 655.0 / 9.8 | 279 | intergenic (‑91/+504) | lacZ/lacI | pseudogene, truncated/DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base T (72/172); new base G (19/13); total (92/187) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.21e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 282 total aligned reads displayed.
GGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGA > CP009273/360008‑360302 | ggCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTGGCt > 1:810094/1‑150 (MQ=255) ctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttgtatttgatgcctggggaattAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTc < 2:1399808‑M1/56‑1 (MQ=255) aCTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTGGCTTCg > 2:278836/1‑150 (MQ=255) cTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGGTGGTTCgg > 1:4485752/1‑149 (MQ=255) ttCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCTGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATT‑CCTGGCAGTTTATGGCGGGCGTCCGGCCCGCCACCCTCCGGGCCGTTGCTTCGCa < 2:1299711/150‑1 (MQ=255) tcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATT‑CCTGGAAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCaa < 2:1127441‑M1/60‑1 (MQ=255) caggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAAc < 2:679144‑M1/61‑1 (MQ=255) caggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAAc < 2:4805177‑M1/61‑1 (MQ=255) cACCGCTTGCCAGCGGCTAACCAACCCGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTAAAACATTATAATTCCGAGCCCAACTT‑CCTGGCTTTTTTGGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAAc < 1:4756966/150‑1 (MQ=25) aCCGCTTGCCCGGGGCTCCCCAGCCCGCCCCCCCAGCCTGTGCCGGAGCGCCTATGATACGCTGACAAACTTCAGACTCCCGAGCCCCATGT‑CCTGGCTGTTTTGGGGGGGGGTCCGGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACg < 1:4733827/150‑1 (MQ=12) gagagcgttcaccgacaaacaacagataacacgaacggcccagtctttcgactgcgcctttcgttttattggcggccgggggaattAATG‑CCGGGCTGTTTAGGGCGGGCGGCCGGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGtt > 1:1888632‑M1/87‑150 (MQ=255) agcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCaa > 2:3140565‑M1/84‑150 (MQ=255) gCCAGCGGCTTACCATCCAGCGCCACCATCCCGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATG‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAAt < 2:3881344/150‑1 (MQ=255) gCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAAt > 1:3424895/1‑150 (MQ=255) cAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATT‑CCTGGCAGTTTTTGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATcc < 2:516205/150‑1 (MQ=255) accgacaaccaacagagaaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCt > 2:1505435‑M1/77‑150 (MQ=255) accgacaaacaccagataaaacgaacggccctgtctttcgaatgagcatttctttttatttgatgcatgggtaattAATT‑CCTGGCAGTTTAGGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCt > 1:3636928‑M1/77‑150 (MQ=255) cgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTcc < 2:3132888‑M1/76‑1 (MQ=255) attACCATCCAGCGCAACAACCCAGGGCTGGAGCTCGGTTGATAAGCTGTCAAACATGTGAATGCCGAACCCAATTT‑CCTGGCATTTTTTGGCGGGCGTCGTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTccc < 1:3132018/149‑1 (MQ=18) aaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttattggatgcctggggaattAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCcggc < 2:587548‑M1/80‑1 (MQ=255) aCCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCcggc > 2:2690011/1‑150 (MQ=255) ccATCCAGCGCCACCTGCCAGTGCAGGAGCTCGGTTGATAAGCTGTCAAACATTTGAATTCCGATCCCAATTT‑CCTGGAAGTTTATGGCGGGCGTCCTGCCGGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCcggcg < 2:4084527/150‑1 (MQ=21) ccATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGGGGCTTCGCAACGTTCAAATCCCCCCCcggcg > 2:4552647/1‑150 (MQ=255) aacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggacttAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCACCGGTCCAGTCCGCGCCcggcg < 2:4337781‑M1/81‑1 (MQ=255) cATCCCGCGCCACCATCCTGTGCAGGAGCTCCTATGATATGCTGTCAAACATGATAATTCCGATCCCAAATT‑CCTGGCTTTTTTTGGGGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCcggcgg < 2:1609256/150‑1 (MQ=21) cATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTGGGGTGGCCACCGTCAAATACCCGCCcggcgg > 1:2528018/1‑150 (MQ=37) cAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGAttt < 2:3195400/150‑1 (MQ=255) gcgcCACCATCCAGTCCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGt < 2:3365072/150‑1 (MQ=255) caccaTCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCGTAGCAAACTTAAAAAACGCACCCCGCGGATTTGttcaa > 1:1425771/1‑146 (MQ=25) accaTCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAAAGTTAAAATACGCTACCGGCGGATTTGTCCTaa > 2:3025782/1‑149 (MQ=255) aacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTAc < 2:4709746‑M1/93‑1 (MQ=255) aacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTAc > 1:2294506‑M1/58‑150 (MQ=255) aTCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCTATCCCCAATT‑CCGGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCa < 1:4413347/150‑1 (MQ=255) aTCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCa < 1:1962779/150‑1 (MQ=255) tCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATG‑CGTGGCTGTTTTTGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAg < 2:16174/150‑1 (MQ=255) aGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATT‑CCTGGCAGTTTATGGCGGGCGTCATGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGgag > 2:2656127/1‑150 (MQ=255) tGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTTCGTAGCAACGGTCAAATACCCTACCCGCGGATTTTTGCTACACAGGagag > 1:1062941/1‑150 (MQ=21) ccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGc < 2:1150188‑M1/103‑1 (MQ=255) aGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGt < 2:394922/150‑1 (MQ=255) gtctttcgcctgagcttttttttttatttgttgcctgggtaattATTT‑CCGGGCTGTTTTTGGGGGGCGTCCGGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGtt > 1:550460‑M1/45‑150 (MQ=255) gtctttcgactgagcctttcgttttatttgatgcctggggaattAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAAAAGTCAAATACGCTACCCGCCGCTTTGTCCAAAACACGAGAGCGtt < 1:2743732‑M1/106‑1 (MQ=255) tttcgactgagcctttcgttttatttgatgcctggggaattAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCGTCGCAACGTTCAAATACGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCAc < 2:1884422‑M1/109‑1 (MQ=255) gCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAAGT‑CCTGGCAGTTTTTGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCAc < 1:1559056/150‑1 (MQ=255) ctcgattgcgcttgtcgatttatttgaggcctgggtaattAATT‑CCGGGCTGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCAcc > 1:2977297‑M1/41‑150 (MQ=255) cTCCGATGATATGGTTTCAAACATGAGAATTCCGACCCCCAAGT‑CCTGGCTTTTTTGGGCGGGCGTCCTCCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGCTCAcc < 1:2116077/150‑1 (MQ=17) cTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCAcc < 2:3047389/150‑1 (MQ=255) gatgatAAGCTGTCAAACATGAGAATTCCGATCCCCAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGaca > 2:2694202/1‑150 (MQ=255) atgatAAGCTGTCAAACATGAGAATTCCGATCCCCAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGacaa < 1:3246520/150‑1 (MQ=255) agcctttcgttttatttgatgcctggggaattAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGacaaaca < 2:289581‑M1/118‑1 (MQ=255) gcctttcgttttatttgatgcctggggaattAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGaccaacaa < 2:2593597‑M1/119‑1 (MQ=255) tttcgttttatttgatgcctggggaattAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCGTCGCAACCTGCAAATACGCTACCGGCGGCTTTTTCCAACTCAGGAGAGCGGTGACCGACAAACAACAg < 1:2404236‑M1/122‑1 (MQ=255) gCTGTAAAACATGAGAATTCCGATCCCCAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAg < 2:2074127/150‑1 (MQ=255) cTGTCAAACATGAGAATTCCGATCCCCAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGa < 2:4277290/150‑1 (MQ=255) tGTCAAACATGAGATTTCCGATCCCCAATT‑CCTGGCTGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGAt < 1:1466467/150‑1 (MQ=255) tGTCAAACATGAGAATTCCGATCCCCAATT‑CCTGGCAGTTTTTGGCGGGCGTCCGGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGAt < 1:1466350/150‑1 (MQ=255) tGTCAAACATGAGAATTCCGATCCCCAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCGTAGCCACCTTAAAATCCGCTCCCGGCGGATTTGTCCGACGCAGGAGAGCGTTCACCGCCAAACAACAGaa > 1:754184/1‑149 (MQ=17) gttttatttgatgcctggggaattAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCACTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATaa > 2:2728159‑M1/25‑150 (MQ=255) gttttatttgatgcctggggaattAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAAAGTTCAAATCCGCACCCGGCGGATTTGTCCAACTCAGGAGAGCGTTCACCGACAAACAAcagaaaa < 2:2325804‑M1/126‑4 (MQ=255) aaaattGATAATGCCGATCCCCAATG‑CGTGGCTTTTTAGGGGGGGGGTCCGGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATaaaa < 1:902623/145‑1 (MQ=11) aaaCATGAGAATTCCGATCCCCAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATaaaa < 2:4241469/150‑1 (MQ=255) ttatttgatgcctggggaattAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGGTAAAATACGCTCCCCGCGGAGTTGTGCTACAAAGGAGTGCGTTCACCCACAAACAACAcaaaaaaa < 1:650219‑M1/129‑2 (MQ=255) ttatttgatgcctggggaattAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTAGCAAAGTTAAAATACCCACCCCGCGGATTTGTGCTACACAGGAGAGCGGTCACCGACAAACAAACGAtaaaaa < 1:3984247‑M1/129‑2 (MQ=255) tatttgatgcctggggaattAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGCGCGTTCACCGACAAACAACAGATAAAACg < 1:1117120‑M1/130‑1 (MQ=255) acttGAGAATTCCGATCCCCAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACg < 2:3300546/147‑1 (MQ=255) aCATGAGAATTCCGATCCCCAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACg < 2:3563818/150‑1 (MQ=255) cATGAGAATTCCGATCCCCAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGa > 2:3473016/1‑150 (MQ=255) cATGAGAATTCCGATCCCCAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTGGCGTGCCAAAGGTCAAATACGCACCCCGCGGATTTTTGCTAATGAGGAGATCGTTGACCGAAAAAAAACAGAAAAAAAGa > 1:1024547/1‑150 (MQ=17) tgcgtgggtaattAATT‑CGTGGCAGTTTATGGGGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGcc > 2:3251507‑M1/14‑150 (MQ=255) ttCCGATCCCCAATT‑CCGGGCAGTTTTTGGGGGGCGTCAGGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCa < 1:2779369/150‑1 (MQ=14) cctggggaattAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGGGTCGCAAACTTAAAAAACGCTCCCCGCGGATTTGTCCTAAACAGGAGAGCGTTCACCGACCAAAAACAGAAAAAAAGAAAGGCCCa < 1:1141603‑M1/139‑1 (MQ=255) ccGATCCCCAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGt < 2:902001/150‑1 (MQ=40) ccGATCCCCAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGt < 2:1233568/150‑1 (MQ=40) gcgccccAAGT‑CCGGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCt < 2:3657406/147‑1 (MQ=16) tCCCCAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAAAGTTAAAATACCCTACCCGCGGATTTTTCCTACTCAGGAGTGCGTTAACAGACAAACAAAAGATAAAAAGAAACGACCAGTCttt > 1:1540992/1‑150 (MQ=11) ccccAATG‑CCTGGATGTTTCTGGCGGGCGTCCGGCCCGCCACCCTCCGGGCCGGTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTc < 1:924144/150‑1 (MQ=11) ctcAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCg < 1:3951504/148‑1 (MQ=18) attaaTT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTAGCCACGTTCAAAACCGCAACCGGCGGAATTGGGCGACTCCGGAGAGCGGGGACCCACAAACAACAGATAAAACGAAAGGCCCCCTCTTTCg > 1:2687621/4‑150 (MQ=11) ccAATTCCCGGGCAGTTTTTGGCGGGCGCCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCg < 1:1800981/150‑1 (MQ=11) tactt‑CCTGGCTGTTTATGGCGGGCGTCCTGCCCGCGACCCTCCGGGCCGTTGCGTCGCTAGGTGCAAATCCGCTGCCGGCGGTTTTGTCCTACTCTGGAGAGCGTTCACGGACAACCAACAGATAAAACGAAAGGCCCAGTCTTTCGAc < 2:253501/147‑1 (MQ=11) cAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGAc < 2:1866234/150‑1 (MQ=33) aaTT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGGTCAAATCCGCTCCCGGCGGATTTGTCCTACGCAGGAGAGCGTTGACCGACCAACAACAGATAAAACGCAAGGCCCAGTCTTTAGACt > 2:3736663/1‑150 (MQ=11) aTT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAAc > 2:402078/1‑60 (MQ=35) tt‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTGGGGTCGCAAAGGTCAAAAACGCTCCCGGGGGGTTTGTGCGGAACAGGCGCGCGGTCACCCCCCAACAACCGAAAAAAAGAAAGGCCCCCTCTTTGGACTgg > 2:2018372/1‑149 (MQ=2) t‑CCGGGCAGTTTCTGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAg < 2:4496041/150‑1 (MQ=11) gtttcgggcgggcgTCCGGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGt < 1:2917341/144‑1 (MQ=0) cccgCCCCCCACCCTCCGGGCCGTCGCCTCGCAACGGACAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTg < 2:1263996/147‑1 (MQ=1) tGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCGTTCGGTTTATTTGATGCCTggg > 2:3895945/1‑150 (MQ=11) ccGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGgggaa < 2:1540992/150‑1 (MQ=32) ccccACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGgggaat < 1:2866430/148‑1 (MQ=18) cGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCGGgggaat > 2:360981/1‑150 (MQ=18) gCCACCCTCCGGGCCGTTGCGTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGgggaatt < 2:3523518/150‑1 (MQ=21) catcccGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCTGCGGATTTGTCCTACTCAGGAGTGCGTTCACCGACAAAAAACAGATAAAACGAAAGGCCCAGTCTTTCTACTGAGCCTTTCGTTTTATTTGATGCCTGgggaattggga < 1:4175548/146‑1 (MQ=11) gggCCGTTGCTTCGCAACGTTCAAATCCCCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTACACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGaattaat < 1:4047950/150‑1 (MQ=255) ggCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGaattaatt < 1:2713642/150‑1 (MQ=255) ccGCTGCTTCGCAACGCTCAAATCCGCTCCCCGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGCTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCt < 2:3984247/150‑1 (MQ=255) cGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGACTTGGGCCTTAATTCtt > 2:1797515/1‑150 (MQ=255) ttGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGACTTAATTCTTGa > 2:4765679/1‑150 (MQ=255) ttGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGa > 2:4777559/1‑150 (MQ=255) cTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGgggaat > 1:4846406/1‑130 (MQ=34) cTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGgggaat < 2:4849460/130‑1 (MQ=34) | GGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATT‑CCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGA > CP009273/360008‑360302 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |