Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 399,156 T→A 19.7% intergenic (+99/‑3) psiF → / → yaiC PsiF family protein/diguanylate cyclase, cellulose regualtor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273399,1560TA19.7% 638.9 / 132.0 242intergenic (+99/‑3)psiF/yaiCPsiF family protein/diguanylate cyclase, cellulose regualtor
Reads supporting (aligned to +/- strand):  ref base T (107/87);  new base A (33/15);  total (140/102)
Fisher's exact test for biased strand distribution p-value = 1.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 246 total aligned reads displayed.

GGTGATGATCGTAATAAGTTTATGAGTGCCTGCCTCAAGAAAGCCGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCTTCGCCCGTCGCGTCAGACTACCCCGTGCGGTTGGCCTG  >  CP009273/399007‑399305
                                                                                                                                                     |                                                                                                                                                     
 gTGATGATCGTAATAAGTTTATGAGTGCCTGCCTCAAGAAAGCCGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTg                                                                                                                                                      >  2:1389613/1‑150 (MQ=255)
  tgatgaTCGTAATAAGTTTATGAGTGCCTGCCTCAAGAAAGCCGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGa                                                                                                                                                     >  1:2046210/1‑150 (MQ=255)
  tgatgaTCGTAATAAGTTTATGAGTGCCTGCCTCAAGAAAGCCGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCAGa                                                                                                                                                     >  1:3044602/1‑150 (MQ=255)
    atgatCGTAATAAGTTTATGAGTGCCTGCCTCAAGAAAGCCGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCAGAAt                                                                                                                                                   <  2:133968/150‑1 (MQ=255)
      gatCGTAATAAGTTTATGAGTGCCTGCCTCAAGAAAGCCGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGt                                                                                                                                                 <  2:3027869/150‑1 (MQ=255)
           taataaTTTTATGAGTGCCTGCCTCAAGAAAGCCGCATGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCa                                                                                                                                            <  1:4104446/150‑1 (MQ=255)
           taataaGTTTATGAGTGCCTGCCTCAAGAAAGCCGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCa                                                                                                                                            <  2:4398971/150‑1 (MQ=255)
              taaGTTTATGAGTGCCTGCCTCAAGAAAGCCGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCaaaa                                                                                                                                         >  1:2200192/1‑150 (MQ=255)
                  tttATGAGTGCCTGCCTCAAGAAAGCCGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAaataa                                                                                                                                     <  1:3194966/150‑1 (MQ=255)
                   ttATGAGTGCCTGCCTCAAGAAAGCCGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAaataat                                                                                                                                    <  1:123897/150‑1 (MQ=255)
                    tATGAGTGCCTGCCTCAAGAAAGCCGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATaatg                                                                                                                                   <  2:2507902/150‑1 (MQ=255)
                     aTGAGTGCCTGCCTCAAGAAAGCCGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATaatga                                                                                                                                  <  2:4843631/150‑1 (MQ=255)
                      tGAGTGCCTGCCTCAAGAAAGCCGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCAGAATGTTCCCAAAAATaatgaa                                                                                                                                 >  1:3695682/1‑150 (MQ=255)
                           gcctgcctCAAGAAAGCCGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAatgatg                                                                                                                            <  1:3247614/150‑1 (MQ=255)
                                 ctCAAGAAAGCCGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACt                                                                                                                      >  1:877163/1‑150 (MQ=255)
                                 ctCAAGAAAGCCGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACt                                                                                                                      <  1:2361009/150‑1 (MQ=255)
                                  tCAAGAAAGCCGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATCTCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCAGAATGTTCCCAAAAATAATGAATGATGAAAACtt                                                                                                                     <  2:2614312/150‑1 (MQ=255)
                                   cAAGAAAGCCGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACttt                                                                                                                    <  1:188118/150‑1 (MQ=255)
                                   cAAGAAAGCCGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACttt                                                                                                                    <  1:646155/150‑1 (MQ=255)
                                       aaaGCCGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCa                                                                                                                >  1:847692/1‑150 (MQ=255)
                                        aaGCCGCCTGATACCTGATAGTGCTAACGGGTGTGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCaa                                                                                                               <  1:1091318/150‑1 (MQ=255)
                                        aaGCCGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCaa                                                                                                               <  2:1456219/150‑1 (MQ=255)
                                         aGCCGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCAGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCaaa                                                                                                              <  1:3340367/150‑1 (MQ=255)
                                           ccgccTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCaaaaa                                                                                                            >  1:2535732/1‑150 (MQ=255)
                                           ccgccTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCaaaaa                                                                                                            >  1:1542383/1‑150 (MQ=255)
                                             gccTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAg                                                                                                          <  2:642495/150‑1 (MQ=255)
                                             gccTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCAGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAg                                                                                                          <  2:246423/150‑1 (MQ=255)
                                              cctgatacctgataGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAgc                                                                                                         >  2:210714/1‑150 (MQ=255)
                                                 gatacctgataGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCAGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAgcggc                                                                                                      <  1:106681/150‑1 (MQ=255)
                                                      ctgataGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGgcgc                                                                                                 >  1:2030375/1‑150 (MQ=255)
                                                      ctgataGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTCCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCAGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGgcgc                                                                                                 <  2:1040125/150‑1 (MQ=255)
                                                       tgataGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTGAAAAAAGCGGCGGCGCa                                                                                                >  2:4537368/1‑150 (MQ=255)
                                                         ataGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCAGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACg                                                                                              >  2:3481448/1‑150 (MQ=255)
                                                            gTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCAGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACgggg                                                                                           >  2:2669477/1‑150 (MQ=255)
                                                             tGCTAACGGGTTAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGgga                                                                                          >  1:1966864/1‑150 (MQ=255)
                                                             tGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCATTTAGCTCCGTCTCTATATTTTGGGAAAATTGTTTCAGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGgga                                                                                          <  2:3311476/150‑1 (MQ=255)
                                                              gCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAATAAAGCGGCGGCGCACGGggag                                                                                         >  2:3978818/1‑150 (MQ=255)
                                                                tAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGggagga                                                                                       >  2:1848209/1‑150 (MQ=255)
                                                                 aaCGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGaa                                                                                      >  2:4239328/1‑150 (MQ=255)
                                                                           cTACGAAAATGGCACACCCTAAATATCATACTTCTGCATTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTaa                                                                            <  2:4159995/150‑1 (MQ=255)
                                                                            tACTAAAATGGCACACCCGAAATATCATACTTCTGCCTTTAGCCCCGTATATATAATTTGGGAAAATTTTTTAAGAATGTTCCCAAAAATAATTAATGATTAAAAATTTTTAAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAAc                                                                           <  1:556676/150‑1 (MQ=255)
                                                                              cGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATCAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAActc                                                                         <  1:3940167/150‑1 (MQ=255)
                                                                               gAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAActcc                                                                        >  2:938791/1‑150 (MQ=255)
                                                                                aaatTGGCCAACCCTAAATATAAAACTTCTGCCTTTCGCTCCTTATCTATATTTTGTGAAATTTTTTTCTGAATGTTCCAAAAAATAATGAATTATTAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAActcct                                                                       <  1:506811/150‑1 (MQ=255)
                                                                                 aatgggCCCACCCTAAATATCATACTTCTGACTTTAGCTCCGTCTCTATATTTGGGTAAATTTTTTTCAGATTGTTCCAAAAAATAATTAATGATTAAAACTTTTACAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAActcctc                                                                      <  1:2898486/146‑1 (MQ=255)
                                                                                 aaaTGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCTTCTCTATAATTTGGGAAAATTGTCTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAActcctc                                                                      <  1:3533086/150‑1 (MQ=255)
                                                                                  aaTGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCa                                                                     >  2:4191899/1‑150 (MQ=255)
                                                                                   aTGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCaa                                                                    >  1:3005592/1‑150 (MQ=255)
                                                                                    tGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCaaa                                                                   >  1:3475690/1‑150 (MQ=255)
                                                                                    tGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCGCaaa                                                                   >  1:3475778/1‑150 (MQ=255)
                                                                                      gCTCACCCGAAATATCATACTTCTGCCTCTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAAc                                                                 <  2:1958574/150‑1 (MQ=255)
                                                                                        tCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCAGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGa                                                               >  1:275995/1‑150 (MQ=255)
                                                                                          aCCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCAGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAAc                                                             >  1:405767/1‑150 (MQ=255)
                                                                                           cccGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACa                                                            >  1:1052606/1‑150 (MQ=255)
                                                                                            ccGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACAt                                                           >  2:1625205/1‑150 (MQ=255)
                                                                                                     cATACTTCTGCGTTTAGCTCCGTCTATTTAATTTGGGAAATTTGTTTCTGAATTTTCCAAAAAATAATTAATTATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTcc                                                  <  2:3660605/150‑1 (MQ=255)
                                                                                                     cATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCAGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTcc                                                  >  2:3086362/1‑150 (MQ=255)
                                                                                                        aCTTCTGCCTTTAGCTCGGTATCTATATTTTGGTAAATTTTTTTCTGAATGTTCCCAAAAATAATGATTTATGAAAATTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCggg                                               <  2:420082/150‑1 (MQ=255)
                                                                                                        aCTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCggg                                               >  2:753650/1‑150 (MQ=255)
                                                                                                         cTTCTGCCTTTAGCTCCGTCTCTACAATTTGGGAAAATTGTTTCAGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGc                                              >  2:3021622/1‑150 (MQ=255)
                                                                                                          ttCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCAGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCt                                             >  2:387922/1‑150 (MQ=255)
                                                                                                           tCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTg                                            <  2:1882279/150‑1 (MQ=255)
                                                                                                                 tttAGCTCCGTCTCTATAATTTGTGAAAATTGTTTCTGAATGTTCCAAAAAATAATTAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCTTc                                      <  1:3536664/150‑1 (MQ=255)
                                                                                                                 tttAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCTTc                                      <  2:2825684/150‑1 (MQ=255)
                                                                                                                     gCTCCGTCTCTATAATTTGGTAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCTTCGccc                                  <  2:4431058/150‑1 (MQ=255)
                                                                                                                        cagtatctATAATTTGGTAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTAAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCTTCGCCCGTc                               <  2:1457117/145‑1 (MQ=255)
                                                                                                                         cGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCTTCGCCCGTcg                              >  2:2771242/1‑150 (MQ=255)
                                                                                                                          gTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCTTCGCCCGTcgc                             >  2:4694259/1‑150 (MQ=255)
                                                                                                                          gTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCTTCGCCCGTcgc                             >  2:168117/1‑150 (MQ=255)
                                                                                                                           tctcTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGACCCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCGTCGCCCGTcgcg                            >  1:214915/1‑150 (MQ=255)
                                                                                                                            ctctATAATTTGGGAAAATTGTTTCAGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCTTCGCCCGTCGCGt                           >  1:2258480/1‑150 (MQ=255)
                                                                                                                             tctATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCTTCGCCCGTcgcggc                          >  1:128417/1‑148 (MQ=255)
                                                                                                                              ctATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCTTCGCCCGTCGCGTCa                         >  2:1672672/1‑150 (MQ=255)
                                                                                                                               tataATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCTTCGCCCGTCGCGTCAg                        >  2:500773/1‑150 (MQ=255)
                                                                                                                               tataATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCTTCGCCCGTCGCGTCAg                        <  2:1880395/150‑1 (MQ=255)
                                                                                                                                 taATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCGGCGGTCCGGGCTGCGCTTCGCCCGTCGCGTCAGAc                      >  2:2310194/1‑150 (MQ=255)
                                                                                                                                 taATTTGGGAAAATTGTTTCAGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCTTCGCCCGTCGCGTCAGAc                      >  1:4849468/1‑150 (MQ=255)
                                                                                                                                  attttGGGAAAATTGTTTCTTAATGTACCAAAAAATAATGAATTATGAAAACTTTTACAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCTTCGCCCGTCGCGTCAGACt                     <  1:469464/148‑1 (MQ=255)
                                                                                                                                  aaTTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCTTCGCCCGTCGCGGCAGACt                     >  1:4797288/1‑150 (MQ=255)
                                                                                                                                   aTTTGTGAAATTTGTTTCTGAATGTTCCCAAAAATATTGAATGATTAAAAATTTTTAAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCTTCGCCCGTCGCGTCAGACTa                    <  1:1712014/150‑1 (MQ=255)
                                                                                                                                   aTTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCTTCGCCCGTCGCGTCAGACTa                    >  2:1195966/1‑150 (MQ=255)
                                                                                                                                   aTTTGGGAAAATTGTTTCTGAATGTCCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCTTCGCCCGTCGCGTCAGACTa                    <  1:3070779/150‑1 (MQ=255)
                                                                                                                                     ttGGGAAAATTGTTTCAGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCTTCGCCCGTCGCGTCAGACTAcc                  >  2:2208086/1‑150 (MQ=255)
                                                                                                                                       gggAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCTTCGCCCGTCGCGTCAGACTAcccc                <  2:770999/150‑1 (MQ=255)
                                                                                                                                            aaTTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCTTCGCCCGTCGCGTCAGACTACCCCGTGCg           >  1:3089774/1‑150 (MQ=255)
                                                                                                                                             aTTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCTTCGCCCGTCGCGTCAGACTACCCCGTGCgg          >  2:4678938/1‑150 (MQ=255)
                                                                                                                                             aTTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCTTCGCCCGTCGCGTCAGACTACCCCGTGCgg          >  2:1041861/1‑150 (MQ=255)
                                                                                                                                             aTTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCTTCGCCCGTCGCGTCAGACTACCCCGTGCgg          >  1:2423234/1‑150 (MQ=255)
                                                                                                                                             aTTGTTTCAGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCTTCGCCCGTCGCGTCAGACTACCCCGTGCgg          >  2:822380/1‑150 (MQ=255)
                                                                                                                                              ttGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCTTCGCCCGTCGCGTCAGACTACCCCGTGCGGt         <  1:1502905/150‑1 (MQ=255)
                                                                                                                                              ttGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCTTCGCCCGTCGCGTCAGACTACCCCGTGCGGt         >  1:3135236/1‑150 (MQ=255)
                                                                                                                                               tGTTTCTTAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCTTCGCCCGTCGCGTCAGACTACCCCGTGCGGtt        <  1:1474863/150‑1 (MQ=255)
                                                                                                                                               tGTTTCTGAATGTTCCAAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCTTCGCCCGTCGCGTCAGACTACCCCGTGCGGtt        <  1:1475667/150‑1 (MQ=255)
                                                                                                                                                ttttCTGAATTTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCTTCGCCCGTCGCGTCAGACTACCCCGTGCGGTTg       <  1:2629144/149‑1 (MQ=255)
                                                                                                                                                   tCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCTTCGCCCGTCGCGTCAGACTACCCCGTGCGGTTGGcc    >  1:4272629/1‑150 (MQ=255)
                                                                                                                                                   tCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCTTCGCCCGTCGCGTCAGACTACCCCGTGCGGTTGGcc    >  1:3609745/1‑150 (MQ=255)
                                                                                                                                                    cTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCTTCGCCCGTCGCGTCAGACTACCCCGTGCGGTTGGCct   <  2:380504/150‑1 (MQ=255)
                                                                                                                                                    cTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCTTCGCCCGTCGCGTCAGACTACCCCGTGCGGTTGGCct   >  2:4731879/1‑150 (MQ=255)
                                                                                                                                                     tGATTGTCCCAAAAAATATGTAATGATAAAAAGTTTTAAAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCTTCGCCCGTCGCGTCAGACTACCCCGTGCGGTTGGCctg  <  1:4489059/150‑1 (MQ=255)
                                                                                                                                                     tGAATGTTCCCAAAAATAATTAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCTTCGCCCGTCGCGTCAGACTACCCCGTGCGGTTGGCctg  <  1:975179/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGTGATGATCGTAATAAGTTTATGAGTGCCTGCCTCAAGAAAGCCGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCTTCGCCCGTCGCGTCAGACTACCCCGTGCGGTTGGCCTG  >  CP009273/399007‑399305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑