| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 3,626,088 | 0 | T | G | 57.9% | 6.5 / 15.9 | 19 | V168G (GTC→GGC) | hcf136_3 | Ycf48‑like protein |
| Reads supporting (aligned to +/- strand): ref base T (6/2); new base G (1/10); total (7/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.27e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.46e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGGAGGTGCAGGCAGCCGAGGCTGCCGCAGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGT‑CGGTGCCTACGGGGTGGCCAT‑GCGCACCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGC > ACB122/3625962‑3626206 | gggAGGGGCAGGCCGCCGAGGCGGCCGCAGACGAGCGGCGCCGGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGT‑CGGTGc < 2:203221/135‑1 (MQ=255) gcagCCGAGGCTGCCGCAGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGT‑CGGTGCCTACGGGGTg > 6:231114/1‑135 (MQ=255) cgCCGCGGCGGCGGAAGAGGCGCGGCGCGGGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGT‑CGGTGCCTACGGGGTGGcc < 8:132820/135‑1 (MQ=255) aGGCTGCCGCAGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCACCGGCCTGGTCGT‑GCGTGCCGACGGGGTGGCCAT‑gcg > 2:131949/1‑136 (MQ=255) ccTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCCCCGCAGCCCCCAACGGCCTGGGCGT‑CCGTGCCGACGGGGGGGGCCA‑GCGCACCG‑CCGCCggcgggcccca > 5:216549/1‑131 (MQ=255) cgcAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGT‑CGGTGCCTACGGGGTGGCCAT‑GCGCACCG‑ACGACGGCGGGCGCAGCTGGCAGTCg > 4:109041/1‑134 (MQ=255) ggtgGGCGACGGCGCGGACAAGCGGTTGTTGCCCGTCAATTCCCCCGAGCCCCCCACCGGCCTGGGGGT‑CGGGGCCACCGGGGGGGCCAG‑GCCCACCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGa < 4:83681/134‑1 (MQ=255) ggCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGgt > 7:212865/1‑63 (MQ=255) ggCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGgt < 8:212865/63‑1 (MQ=255) cgccGGCGCGCACACGCGGTTGTGGCCCCTGAATTCCACCCCGGCCGCCACGGGCGGGGGGGG‑CGGGGCATCGGGGGGGCCCAG‑GCCCCCCC‑CCGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGcc < 5:73574/132‑1 (MQ=255) gcgcgcACACGCGTTTGTGGGCCCTTAATTTCACCGTGCCCCGCACCGGCGGGGGCGG‑CGGGGCCTCGGGGGGGGCCAG‑GCGCCCCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACgg < 1:153958/136‑1 (MQ=255) gcgcgcACACGCCGTTGTGGCCCCTCAATTCCCCCGAGCCCCCCACCGGCCTGGGCGG‑CGGGGCCTCGGGGGGGCCCAT‑GCGCACCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACgg < 3:109041/136‑1 (MQ=255) gcgcGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATTCCCGACACCGCCTGGTCCTACGGT‑CCTAACGGGTGGCCAA‑TAGCAACG‑ACCACCGCCCGCGCAGCAGGCAATCGGATAACGGCCGACAGACGCACgg > 2:137861/1‑136 (MQ=255) cgGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGT‑CGGTGCCTACGGGGTGGCCAT‑GCGCCCCG‑CCGCCGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCg > 4:178916/1‑136 (MQ=255) aCAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGT‑CGGTGCCTACGGGGTGGCCAT‑GCGCCCCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGCCTGCCGAACGGGCGTgg > 4:209598/1‑136 (MQ=255) cAAGCGGTTGTTGGCCCTCAATTCCACCCATGCCCCCACGGGCCGGGGCGG‑CGGGGCCTCGGGGGGGGCCAG‑GCGCCCCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGa < 3:177285/136‑1 (MQ=255) ttgttgGCCCTAAATTTCCCCGAGGCCGCCACCGGCCTGGGCGG‑CGGTGCCTCCGGGGGGGCCAG‑GCCCACCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCAcc < 4:219618/136‑1 (MQ=255) tgttgGCCCTTAATTACCCGGAGGCCCGCACGGGCGGGGGGGG‑GGGGGCCT‑CGGGGGGGCCATGGCGCACCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCAcc < 7:111550/135‑1 (MQ=255) tgtggGCCGTTATTTTCCCCGAGGCCCCCACGGGCCGGGGGGT‑CGGTGCCATGGGGGGGGCACG‑GCGCACGG‑AGGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCAcc < 7:246590/135‑1 (MQ=255) tgtggGCCCTGAATTACACCCAGGCCCCCACGGGCTTGGGGGT‑GGGGGCCT‑CGGGGTGGGCCT‑GGCCCCCGCACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCAcc < 8:154119/135‑1 (MQ=255) ggCCCGCAACGGCCTGGGCGG‑CGGGGCCTCCGGGGGGCCCAT‑GCCCACCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGc < 8:138423/135‑1 (MQ=255) | TGGAGGTGCAGGCAGCCGAGGCTGCCGCAGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGT‑CGGTGCCTACGGGGTGGCCAT‑GCGCACCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGC > ACB122/3625962‑3626206 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |