Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB122138,2100AT26.9% 84.6 / 12.1 53V105E (GTA→GAA) CJ019_00146hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (11/27);  new base T (14/0);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 2.52e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CTGGACCAAGCGAGCGTCATAGCGGATCACCACCTCCACTGCCCCATCCGTGCGGTATTCACTCTGAAAGGCCTGCAGCTCGCCGGCCAGTTCGTAGTCTGCCTGCAGGTTACTGTCGTCAGCGCTCAGGCGCTGTACCTGGCCCTCACGCTGG‑AACCCGTCGAGCA‑AGCGGTTACGCAGCAGCACCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTC‑ACCAC‑ATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGC  >  ACB122/138074‑138340
                                                                                                                                        |                                                                                                                                      
cTGGACCAAGCGAGCGTCATATCGGATCAGCACCTCCACTGCCCCATCAGTGCGGTATTCACTCTGAAAGGCCTGCAGCTCGCCGGCGAGTTCGTAGTCTGCCTGCAGGTTACTGTCGTCAGCGCTGAGGCGCTGTa                                                                                                                                        <  7:52636/137‑1 (MQ=255)
         gcgagcgTCATAGCGGATCACCACCTCCACTGCCCCATCCGTGCGGTATTCACTCTGAAAGGCCTGCAGCTCGCCGGCCAGTTCGTAGTCTGCCTGCAGGTTACTGTCGTCAGCGCTCAGGCCCGGTAGCTGGgccc                                                                                                                               >  2:77497/1‑136 (MQ=255)
          ggagcgTGATAGAGGATCACCACCTCCACTGCCCCATCCGTGCGGTATTCACTCTGAAAGGCCTGCAGCTCGCCGGCCAGTTCGTAGTCTGCCTGCAGGTTACTGTCGTCAGCGCTCAGGCGCTGTACCTGGCCCTc                                                                                                                              <  5:257407/136‑1 (MQ=255)
          cgagcgTCATAGCGGATCACCACCTCCACTGCCCCATCCGTGCGGTATTCACTCTGAAAGGCCTGCAGCTCGCCGGCCAGTTCGTAGTCTGCCTGCAGGTTACTGTCGTCAGCGCTCAGGCGCTGTACCTGGCCCTc                                                                                                                              <  4:248465/137‑1 (MQ=255)
           gagcgTCATAGCGGATCACCACCTCCACTGCCCCATCCGTGCGGTATTCACTCTGAAAGGCCTGCAGCTCGCCGGCCAGTTCGTAGTCTGCCTGCAGGTTACTGTCGTCAGCGCTCAGGCGCTGTTACTGGcccgcc                                                                                                                             >  6:109132/1‑134 (MQ=255)
           gagcgTCATAGCGGATCACCACCTCCACTGCCCCATCCGTGCGGTATTCACTCTGAAAGGCCTGCAGCTCGCCGGCCAGTTCGTAGTCTGCCTGCAGGTTACTGTCGTCAGCGCTCAGGCGCTGTACCAGGCCCGCa                                                                                                                             >  7:301348/1‑137 (MQ=255)
           gagcgTCATAGCGGATCACCACCTCCACTGCCCCATCCGTGCGGTATTCACTCTGAAAGGCCTGCAGCTCGCCGGCCAGTTCGTAGTCTGCCTGCAGGTTACTGTCGTCAGCGCTAACGCCCGGTACCTGGCCCTCa                                                                                                                             >  4:145231/1‑137 (MQ=255)
           gagcgTCATAGCGGATCACCACCTCCACTGCCCCATCCGTGCGGTATTCACTCTGAAAGGCCTGCAGCTCGCCGGCCAGTTCGTAGTCTGCCTGCAGGTTACTGTCGTCAGCGCGCAGGCGGTGTACCTGGCCCGCa                                                                                                                             >  4:368524/1‑137 (MQ=255)
           gagcgTCATAGCGGATCACCACCTCCACTGCCCCATCCGTGCGGTATTCACTCTGAAAGGCCTGCAGCTCGCCGGCCAGTTCATAGTCTGCCTGCAGGTTACTGTCGTGCGCCGGGCGGCGCTGTAACTGGcccgcc                                                                                                                             >  4:93897/1‑134 (MQ=255)
           gagcgTCATAGCGGATCACCACCTACACTGCCCCATCCGTGCGGTATTCACTCTGAAAGGCCAGCAGCTCGCCGGCCAGTTCGTAGTCTGCCTGCAGGTTACTGTCGTCCGCGCAAAGGCGCAGTTCCTGGCCcaca                                                                                                                             >  5:222533/1‑137 (MQ=255)
              cgTCATAGCGGATCACCACCTCCACTGCCCCATCCGTGCGGTATTCACTCTGAAAGGCCTGCAGCTCGCCGGCCAGTTCGTAGTCTGCCTGCAGGTTACTGTCGTCAGCGCTCAGGCGCTGTACCTGGCCCTCACGc                                                                                                                          <  4:36842/137‑1 (MQ=255)
                tCATAGCGGATCACCACCTCCACTGCCCCATCCGTGCGGTATTCACTCTGAAAGGCCTGCAGCTCGCCGGCCTGTTCGTAGTCTGCCTGCAGGTTACTGTCGTCAGCGCTCAGGCGCTGTACCTGGCCCTCACGCTg                                                                                                                        <  3:397313/137‑1 (MQ=255)
                tCATAGCGGATCACCACCTCCACTGCCCCATCCGTGCGGTATTCACTCTGAAAGGCCTGCAGCTCGCCGGCCAGTTCGTAGTCTGCCTGCAGGTTACTGTCGTCAGCGCTCAGGCGCTGTACCTGGCCCTCACGCTg                                                                                                                        <  5:408041/137‑1 (MQ=255)
                 cATAGCGGATCACCACCTCCACTGCCCCATCCGTGCGGTATTCACTCTGAAAGGCCTGCAGCTCGCCGGCCAGTTCGTAGTCTGCCTGCAGGTTACTGTCGTGCGCGCGCAGGGGCGGGGCCTTGCCCGAACGcgtg                                                                                                                       >  1:398743/1‑134 (MQ=255)
                              caccTCCACTGCCCCATCCGTGCGGTATTCACTCTGAAAGGCCTGCAGCTCGCCGGCCAGTTCGTAGTCTGCCTGCAGGTTACTGTCGTGCGCGCGCAGGGGCGGTTCCGGGGCCGCACCCGGG‑GCCCCGGCGAGcc                                                                                                         >  1:133100/1‑136 (MQ=255)
                                      cTGCCCCATCCGTGCGGTATTCACTCTGAAAGGCCTGCAGCTCGCCGGCCAGTTCGTAGTCTGCCTGCAGGTTACTGTCGTGAGCGCGCCGGCGCGGTTCCGGGGCCGACCGCGGG‑GACCCCGCGCGCA‑AGccggtt                                                                                                >  3:119483/1‑132 (MQ=255)
                                      cTGCCCCATCCGTGCGGTATTCACTCTGAAAGGCCTGCAGCTCGCCGGCCAGTTCGTAGTCTGCCTGCAGGTTACTGTCGTCAGCGCTCAGGCGCGGTACCTGGCCCACACCCAGG‑AACCCCTACACCA‑AGCGGTTa                                                                                                >  2:79723/1‑137 (MQ=255)
                                        gCCCCATCCGTGCGGTATTCACTCTGAAAGGCCTGCAGCTCGCCGGCCAGTTCGTAGTCTGCCTGCAGGTTACTGTCGTCAGCGCGCAGGCGCTGTTACGGGCCCGCACGCTGG‑AACCCCTCGAGCA‑AGCGGTTACg                                                                                              >  8:232272/1‑137 (MQ=255)
                                         ccccATCCGTGCGGTATTCACTCTGAAAGGCCTGCAGCTCGCCGGCCAGTTCGTAGTCTGCCTGCAGGTTACTGTCGTCAGCGCTCAGGCGCTGTACCTGGCCCTCACGCTGG‑AACCCGTCGAGCA‑AGCGGTTACgc                                                                                             <  3:274001/137‑1 (MQ=255)
                                           ccATCCGTGCGGTATTCACTCTGAAAGGCCTGCAGCTCGCCGGCCAGTTCGTAGTCTGCCTGCAGGTTACTGTCGTCAGCGCGCAGGCGCTGTAACTGGCCCGCACGCGGG‑AACCCGTCGAGCC‑AGCGGGTACgcaa                                                                                           >  3:74486/1‑136 (MQ=255)
                                                      gTATTCACTCTGAAAGGCCTGCAGCTCGCCGGCCAGTTCGTAGTCTGCCTGCAGGTTACTGTCGTCAGCGCTGCGGGGCGGTTCCTGGCCCTCCCCCTGG‑GACCCCTCGAGCA‑ACCGGTTACCCAGCAGCACCgggg                                                                                >  7:233706/1‑137 (MQ=255)
                                                        aTTCACTCTGAAAGGCCTGCAGCTCGCCGGCCAGTTCGTAGTCTGCCTGCAGGTTACTGTCGTCAGCGCGCAGGCGCTGTACCTGGCCCTCACGCTGG‑AACCCGTCGAGCA‑AGCGGTTACGCAGCAGCACCGGGGcc                                                                              <  5:423948/137‑1 (MQ=255)
                                                               ctGAAAGGCCTGCAGCTCGCCGGCCAGTTCGTAGTCTGCCTGCAGGTTACTGTCGTGCGCGCGCCGGCGGGGTTCCAGGGCCACCCCCGGG‑CACCCGTCGCGCC‑AACCGTTTAGCAGCCCCACCCGGGCCGccaccc                                                                       >  8:184342/1‑132 (MQ=255)
                                                                    aGGCCTGCAGCTCGCCGGCCAGTTCGTAGTCTGCCTGCAGGTTACTGTCGTCAGCGCGCAGGCGCGGTACCTGGCCCTCACGCGGG‑GAACCCTCGAGCA‑ACCCGTTACGCAGCACCACCCGGGCCGCATCCCGCCac                                                                  >  2:45902/1‑136 (MQ=255)
                                                                      gCCTGCAGCTCGCCGGCCAGTTCGTAGTCTGCCTGCAGGTTACTGTCGTCAGCGCTCAGGCGCTGTACCTGGCCCTCACGCTGG‑AACCCGTCGAGCA‑AGCGGTTACGCAGCAGCACCGGGGCCGCATCGCTCCAgcg                                                                <  4:402478/137‑1 (MQ=255)
                                                                      gCCTGCAGCTCGCCGGCCAGTTCGTAGTCTGCCTGCAGGTTACTGTCGTCAGCGCTCAGGCGCTGTACCTGGCCCTCACGCTGG‑AACCCGTCGAGCA‑AGCGGTTACGCAGCAGCACCGGGGCCGCATCGCTCCAgcg                                                                <  1:225324/137‑1 (MQ=255)
                                                                      gCCTGCAGCTCGCCGGCCAGTTCGTAGTCTGCCTGCAGGTTACTGTCGTCAGCGCTCAGGCGCTGTACCTGGCCCTCACGCTGG‑AACCCGTCGAGCA‑AGCGGTTACGCAGCAGCACCGGGGCCGCATCGCTCCAgcg                                                                <  1:79723/137‑1 (MQ=255)
                                                                      gCCTGCAGCTCGCCGGCCAGTTCGTAGTCTGCCTGCAGGTTACTGTCGTCAGCGCTCAGGCGCTGTACCTGGCCCTCACGCTGG‑AACCCGTCGAGCA‑AGCGGTTACGCAGCAGCACCGGGGCCGCATCGCTCCAgcg                                                                <  7:232272/137‑1 (MQ=255)
                                                                      gCCTGCAGCTCGCCGGCCAGTTCGTAGTCTGCCTGCAGGTTACTGTCGTCAGCGCTCAGGCGCTGTACCTGGCCCTCACGCTGG‑AACCCGTCGAGCA‑AGCGGTTACGCAGCAGCACCGGGGCCGCATCGCTCCAgcg                                                                <  6:108691/137‑1 (MQ=255)
                                                                      gCCTGCAGCTCGCCGGCCAGTTCGTAGTCTGCCTGCAGGTTACTGTCGTCAGCGCTCAGGCGCTGTACCTGGCCCTCACGCTGG‑AACCCGTCGAGCA‑AGCGGTTACGCAGCAGCACCGGGGCCGCATCGCTCCAgcg                                                                <  5:193435/137‑1 (MQ=255)
                                                                          gCAGCTCGCCGGCCAGTTCGTAGTCTGCCTGCAGGTTACTGTCGTCCGCGCTAAGGCGCGGGTCCAGGGCCGCCCCCGGG‑CACCCCGCGGGCACACCGGTT‑CGCCCCACCACCCGGGCCGCCACGCGCCcgcgcgcg                                                            >  4:74010/1‑137 (MQ=255)
                                                                          gCAGCTCGCCGGCCAGTTCGTAGTCTGCCTGCAGGTTACTGTCGTCCGCGCGCAGGCGGTGTAGCTGGCCCGCCCCCGGG‑CACCCGTCGCGCA‑AGCGGGTTCGCAGCACCACCGCGGCCGccgcgcgcccgcgcgcc                                                            >  6:118042/1‑136 (MQ=255)
                                                                           cAGCTCGCCGGCCAGTTCGTAGTCTGCCTGCAGGTTACTGTCGTGCGCGCGCAGGCCGGGTTCCAGGCCCGCCCGCGGG‑CACCCCGCGCGCA‑AGCGGTTACGCACCCGCACCCGGGCCCCCACGCTCCAccgcgcgc                                                           >  2:287459/1‑137 (MQ=255)
                                                                           cAGCTCGCCGGCCAGTTCGTAGTCTGCCTGCAGGTTACTGTCGTCAGCGCTCAGGCGCTGTTCCGGGCCCGCACGCTGG‑AACCCGGCGGGCA‑AGCGGTTTAGCAGCACCACCGGGGCCGCAACGCTCCAgcgcccgc                                                           >  7:361444/1‑137 (MQ=255)
                                                                           cAGCTCGCCGGCCAGTTCGTAGTCTGCCTGCAGGTTACTGTCGTCACCCCGCAGGCGCGGTTCCTGGGCCTCACGCGGGCAACCCG‑CGCGCA‑ACCCGGTTCGCCGCCGCCCCCGGGCCCCAGCGCGCccgcccgccc                                                           >  8:270566/1‑135 (MQ=255)
                                                                                 gCCGGCCAGTTCGTAGTCTGCCTGCAGGTTACTGTCGTCCGCGCGCCGGCGCGGGTCCTGGCCCGCACCCGGG‑CACCCGGCGCGCA‑ACCCGGTTCGCAGCCGCCCCCGGGCCGCCCCGCGCCCGCGCCCGCCCgtgt                                                     >  4:83078/1‑137 (MQ=255)
                                                                                   cGGCCAGTTCGTAGTCTGCCTGCAGGTTACTGTCGTGCGCGCTCCGGGGGGGGTCCTGGGCCGCACGCGGG‑GACCCGTCGAGCA‑ACCGGGTACGCCGCAGCACCCGGGCCGCATCCCACCAGCCCGCGcccgggttg                                                   >  4:336283/1‑131 (MQ=255)
                                                                                     gCCAGTTCGTAGTCTGCCTGCAGGTTACTGTCGTCAGCGCTCAGGCGCTGTACCTGGCCCTCACGCTGG‑AACCCGTCGAGCA‑AGCGGTTACGCAGCAGCACCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAgct                                                 <  1:6647/137‑1 (MQ=255)
                                                                                       cAGTTCGTAGTCTGCCTGCAGGTTACTGTCGTCAGCGCTCAGGCGCTGTACCTGGCCCTCACGCTGG‑AACCCGTCGAGCA‑AGCGGTTACGCAGCAGCACCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAgctgc                                               <  6:336192/137‑1 (MQ=255)
                                                                                            agTAGTCTGCCTGCAGGTTACTGTCGTCAGCGCTCAGGCGCTGTACCTGGCCCTCACGCTGG‑AACCCGTCGAGCA‑AGCGGTTACGCAGCAGCACCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTc‑acc                                         <  8:444754/136‑1 (MQ=255)
                                                                                               aGTCTGCCTGCAGGTTACTGTCGTCAGCGCTCAGGCGCTGTACCTGGCCCTCACGCTGG‑AACCCGTCGAGCA‑AGCGGTTACGCAGCAGCACCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTC‑ACcac‑a                                     <  7:184342/137‑1 (MQ=255)
                                                                                                    gcctgcAGGTTACTGTCGTCAGCGCTCAGGCGCTGTACCTGGCCCTCACGCTGG‑AACCCGTCGAGCA‑AGCGGTTACGCAGCAGCACCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTC‑ACCAC‑ATTGcc                                <  2:398743/137‑1 (MQ=255)
                                                                                                      ctgcAGGTTACTGTCGTCAGCGCGAAGGCGCTGTACCTGGCCCACACCCACG‑AACCCCGCGACCA‑AGCCGTTAACCCGCAGCACCCGGGCCCCCAACCTCCAACCCGCCCCCAGTTAACTGCTCAACCAC‑ATTAcca                               >  2:124145/1‑136 (MQ=255)
                                                                                                       tgcAGGTTACTGTCGTCAGCGCTCAGGCGCGGTACCTGGCCCTCACGCTGG‑AACCCCGCGAGCA‑AGCGGTTAAGCAGCAGCCCCCGGGCCCCCACGCTCCCGCGCGCGCCCGTGTAGCTGCTC‑ACCAC‑AATGcccgg                             >  6:169488/1‑135 (MQ=255)
                                                                                                        gcAGGTTACTGTCGTCAGCGCTCAGGCGCTGGTCCAGGGCCACACGCTGG‑AACCCCTCGAGCA‑AGCGGTTAAGCAGCAGCACCGGGGCCGCCTCCAGACAGCGCGCGCCCTTGTAGCTGCTG‑ACCACAATTGccggg                             >  2:122246/1‑134 (MQ=255)
                                                                                                          aGGTTACTGTCGTCAGCGCTCAGGCGCTGTACCTGTCCCTCACGCTGG‑AACCCGTCGAGCA‑AGCGGTTACGCAGCAGCACCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTC‑ACCAC‑ATTGCCCTgggg                          <  5:179506/137‑1 (MQ=255)
                                                                                                          aGGTTACTGTCGTCAGCGCTCAGGCGCTGTACCTGGCCCTCACGCTGG‑AACCCGTCGAGCA‑AGCGGTTACGCAGCAGCACCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTC‑ACCAC‑ATTGCCCTgggg                          <  5:242954/137‑1 (MQ=255)
                                                                                                                cTGTCGTCAGCGCTCAGGCGCTGTACCTGGCCCTCACGCTGG‑AACCCGTCGAGCA‑AGCGGTTACGCAGCAGCACCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTC‑ACCAC‑ATTGCCCTGGGGAACCAc                    <  8:30442/137‑1 (MQ=255)
                                                                                                                cTGTCGTCAGCGCTCAGGCGCTGTACCTGGCCCTCACGCTGG‑AACCCGTCGAGCA‑AGCGGTTACGCAGCAGCACCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTC‑ACCAC‑ATTGCCCTGGGGAACCAc                    <  7:308288/137‑1 (MQ=255)
                                                                                                                          cgcTCAGGCGCTGTACCTGGCCCTCACGCTGG‑AACCCGTCGAGCA‑AGCGGTTACGCAGCAGCACCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTC‑ACCAC‑ATTGCCCTGGGGAACCACGGCAATCCtt          <  1:189409/137‑1 (MQ=255)
                                                                                                                          cgcTCAGGCGCTGTACCTGGCCCTCACGCTGG‑AACCCGTCGAGCA‑AGCGGTTACGCAGCAGCACCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTC‑ACCAC‑ATTGCCCTGGGGAACCACGGCAATCCtt          <  7:270566/137‑1 (MQ=255)
                                                                                                                               aGGCGCTGTACCTGGCCCTCACGCTGG‑AACCCGTCGAGCA‑AGCGGTTACGCAGCAGCACCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTC‑ACCAC‑ATTGCCCTGGGGAACCACGGCAATCCTTGGccc     <  5:451425/137‑1 (MQ=255)
                                                                                                                               aGGCGCTGTACCTGGCCCTCACGCTGG‑AACCCGTCGAGCA‑AGCGGTTACGCAGCAGCACCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTC‑ACCAC‑ATTGCCCTGGGGAACCACGGCAATCCTTGGccc     <  3:83078/137‑1 (MQ=255)
                                                                                                                                  cgcTGTACCTGGCCCTCACGCTGG‑AACCCGTCGAGCT‑AGCGGTTACGCAGCAGCACCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTC‑ACCAC‑ATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGc  <  5:150142/137‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                      
CTGGACCAAGCGAGCGTCATAGCGGATCACCACCTCCACTGCCCCATCCGTGCGGTATTCACTCTGAAAGGCCTGCAGCTCGCCGGCCAGTTCGTAGTCTGCCTGCAGGTTACTGTCGTCAGCGCTCAGGCGCTGTACCTGGCCCTCACGCTGG‑AACCCGTCGAGCA‑AGCGGTTACGCAGCAGCACCGGGGCCGCATCGCTCCAGCGCGCGCCCTTGTAGCTGCTC‑ACCAC‑ATTGCCCTGGGGAACCACGGCAATCCTTGGCCCGGC  >  ACB122/138074‑138340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑