Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,115,4440TG27.5% 112.8 / 11.2 69L242R (CTT→CGT) lptGLipopolysaccharide export system permeaseprotein LptG
Reads supporting (aligned to +/- strand):  ref base T (21/29);  new base G (19/0);  total (40/29)
Fisher's exact test for biased strand distribution p-value = 3.74e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CACTGGCTGCTCGCCGATGTGCGTACCACCTACTTCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGTGCTGCAGCCGATGGTAACGGCGGCCCTGGTGCTGATGGCGATTTCG  >  ACB122/1115308‑1115580
                                                                                                                                        |                                                                                                                                         
cACTGGCTGCTCGCCGATGTGCGTACCACCTACTTCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCt                                                                                                                                           <  6:410747/137‑1 (MQ=255)
 aCTGGCTGCTCGCCGATGTGCGTACCACCTACTTCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCtt                                                                                                                                          >  5:343787/1‑137 (MQ=255)
 aCTGGCTGCTCGCCGATGTGCGTACCACCTACTTCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCtt                                                                                                                                          >  5:93067/1‑137 (MQ=255)
 aCTGGCTGCTCGCCGATGTGCGTACCACCTACTTCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCtt                                                                                                                                          >  7:58063/1‑137 (MQ=255)
            gCCGATCTGCGTACCACCTACTTCCGCGGCGACCACACCTAGGTGGTCAACGCGACGGAGGCCCGCTGGGATGTTTCGCTCACGAGAGATCTGCTCAATACCGTGATCCTCGCGCGGGAGTCGCGTTAGA‑tgagccg                                                                                                                              >  6:364928/1‑131 (MQ=255)
             gcGATTTGGGTACCTCCTACTTCCGGGGCGACCACTGCGAGGTTGTCTACGCTGCGGAGGAGCGCTGGGTTGTTTCGCTAACGCCATAACTTCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCggg                                                                                                                             <  5:123417/136‑1 (MQ=255)
             ccGATGTGCGTACCACCTACTTCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGGCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCggg                                                                                                                             <  3:200455/137‑1 (MQ=255)
             ccGATGTGCGTACCACCTACTTCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCggg                                                                                                                             <  2:41158/137‑1 (MQ=255)
                 ggtgCGTACCACCTACTTCCGCGGCGACCACCCCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGgtgt                                                                                                                         <  5:449685/136‑1 (MQ=255)
                   tgCGTACCACCTACTTCCGCGGCGACCACACCGAGGTGGCCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTgg                                                                                                                       <  8:337024/137‑1 (MQ=255)
                    gCGTACCACCTACTTCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTggg                                                                                                                      <  2:39541/137‑1 (MQ=255)
                    gCGTACCACCTACTTCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTggg                                                                                                                      <  5:439416/137‑1 (MQ=255)
                         ccaccTACTTCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGGATTAc                                                                                                                 <  5:184391/137‑1 (MQ=255)
                           accTACTTCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑tt                                                                                                                                   <  2:177820/117‑1 (MQ=255)
                           accTACTTCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTGTGGA‑tt                                                                                                                                   >  1:177820/1‑117 (MQ=255)
                             cTACTTCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCGTTCGA‑TTTCCGGGTTGTGGGGTTACATcc                                                                                                             >  8:244972/1‑137 (MQ=255)
                              tACTTCCGCGGCGACCACACCGCGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCa                                                                                                            <  1:250475/137‑1 (MQ=255)
                                  tCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTGTGGC‑ATAACGGGTTTTTGGATTAAAAGCactaa                                                                                                        >  2:264741/1‑136 (MQ=255)
                                  tCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCGTTGGA‑TTTACGGGTTGTGGGGTTTAATCCactac                                                                                                        >  7:26137/1‑137 (MQ=255)
                                  tCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCGTTCGA‑TTACCGGGTTGTGGGATTAAATGCcctac                                                                                                        >  4:318257/1‑137 (MQ=255)
                                    cgcgGCGACCACACCGAGGTGGTGAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCACTACtt                                                                                                      <  5:230672/137‑1 (MQ=255)
                                     gcggcgACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCGTTGGA‑TTACCGGGTTGTGGGATTACATCCACGACATg                                                                                                     >  6:201929/1‑137 (MQ=255)
                                         cgACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGGTTTAAATCCACTAATTGTCTg                                                                                                 >  7:239544/1‑137 (MQ=255)
                                              acacCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTGGA‑TTAACGGGTTGTGGGATTACATCCACTCCTTGTCTGATCAg                                                                                            >  8:79038/1‑137 (MQ=255)
                                              acacCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTGGA‑TTAAACGGTTGTGGGGTTTGATCACCTACTtgtgtggtaat                                                                                            >  1:127977/1‑132 (MQ=255)
                                              acacCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAg                                                                                            <  2:422562/137‑1 (MQ=255)
                                                    aGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCGTTGGA‑TTTACGGGTTGTTGGTTTTAATCCACTACTTTTCTGAGAAGGGCCTg                                                                                      >  2:91364/1‑137 (MQ=255)
                                                       tggtCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCGTTGGA‑TTTCCGGGTTGTGGGTTTTAATCCACAACGTGTGGGATAATGGCCGGaca                                                                                   >  6:54588/1‑135 (MQ=255)
                                                        ggtCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCCGGTTTTGGGATTAAATACACTACGTGTGTGATCACGGCCTGaaca                                                                                  >  5:448915/1‑137 (MQ=255)
                                                          tCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGaacaac                                                                                >  6:84047/1‑137 (MQ=255)
                                                          tCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCGTTCGA‑TTAACGGGTTGTGGGTTTTAAAACACTAAATTTCTGATAACGGGCTGaaacac                                                                                >  4:305439/1‑133 (MQ=255)
                                                            aaCGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTAACGCGTTGTGGGATTAAATACAATAATAGTCTGATCATGGCCTGAAAAAATc                                                                              >  8:134119/1‑137 (MQ=255)
                                                                 gcCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCGTTGGG‑TTACCGGGTTGTGGGGTTTAATACACTACTTGTGTGATCAGGGCCTGGAAAACTCCCGcg                                                                         >  3:276868/1‑136 (MQ=255)
                                                                              tGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCCCTACTTGTATGATAAGGGGCTGAACAACTCCCGCTACTGGCTGGGGtt                                                            >  4:123603/1‑137 (MQ=255)
                                                                              tGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAATCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTActgg                                                                    <  4:72418/129‑1 (MQ=255)
                                                                              tGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAATCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTActgg                                                                    >  3:72418/1‑129 (MQ=255)
                                                                                ggATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCGTTCGA‑TTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCt                                                          >  2:31443/1‑137 (MQ=255)
                                                                                   tGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGa                                                       <  4:263280/137‑1 (MQ=255)
                                                                                             aCGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCGTTGGC‑TTTCCGGGTTGTGGGATTAAATCCACTAATTTTCTGATAAGGGGCTGAACAACTCCCCCTAATGGCGGGCGTTCTGGACCAAGGtgct                                             >  6:396531/1‑137 (MQ=255)
                                                                                               gCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGtgctgc                                           <  3:79554/137‑1 (MQ=255)
                                                                                                ccAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGTGCTGCa                                          <  6:343787/137‑1 (MQ=255)
                                                                                                ccAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGTGCTGCa                                          <  1:91364/137‑1 (MQ=255)
                                                                                                ccAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGTGCTGCa                                          <  5:84047/137‑1 (MQ=255)
                                                                                                 cAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGTGCTGCAg                                         <  7:376909/137‑1 (MQ=255)
                                                                                                         cTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGTGCTGCAGCCGATGGt                                 <  6:93067/137‑1 (MQ=255)
                                                                                                         cTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGTGCTGCAGCCGATGGt                                 <  8:58063/137‑1 (MQ=255)
                                                                                                            aaTACCGTGTTCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGTATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGTGCTGCAGCCGATGGTAAc                              <  5:230141/137‑1 (MQ=255)
                                                                                                            aaTACCGTGATCCTCGCGCCGGAGTCGCTTTTGA‑TTACCCGGGTTTGGGATTAAAAACACATAATGTCTGTAAAGGGCCTGCAACACTCCCGCTACTGGGGGGCCGTCTTGACCAAGTTGGGTCAGCCCCTGGTaaa                              >  4:309056/1‑136 (MQ=255)
                                                                                                            aaTACCGTGATCCTCGCGCCGGAGTCGCTTTGGA‑TTACCGGGTTGTTGGATTTCATACCCTACTTGTCTGATAAGGGGCTGAACAAATCCCGCTACTGGCTGGGGTTATGGACCAAGGTGCTGCCCCCGCTGGttaa                              >  8:288264/1‑134 (MQ=255)
                                                                                                            aaTACCGTGATCCTCGCGCCGGAGTCGCTTTGGA‑TTAACGGGTTGTGGGATTACATCCACTACTTGTGTGATAAGGGCCTGAACAACAACCGCTACTGGCTGGCGTTGTGGACCAAGGTGCTTCAGCCGATGGTAAc                              >  4:339616/1‑137 (MQ=255)
                                                                                                            aaTACCGTGATCCTCGCGCCGGAGTCGCTTTGGA‑TTAACGGGTTGTGGGATTACATCCACTACTTGTCTGATCATGGGCTGCACAAATACCGCGACTGGCTGGCGGTCTGGACCAAGGTGGTGCAGCCGAAGGTaaa                              >  4:170988/1‑136 (MQ=255)
                                                                                                            aaTACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTAcc                                                                                                                                >  8:433945/1‑39 (MQ=255)
                                                                                                            aaTACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTAcc                                                                                                                                <  7:433945/39‑1 (MQ=255)
                                                                                                            aaTACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGTATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGTGCTGCAGCCGATGGTAAc                              <  1:13340/137‑1 (MQ=255)
                                                                                                            aaTACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGTGCTGCAGCCGATGGTAAc                              <  1:240569/137‑1 (MQ=255)
                                                                                                            aaTACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TCACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGTGCTGCAGCCGATGGTaaa                              >  6:66455/1‑136 (MQ=255)
                                                                                                            aaTACCGTGATCCTCGCGCCGGAGTCGCTTTAGA‑TTACCGGGGTGTGTGCTTAACTACACTAAGAGTCTGATCAGTGCCAGTAAAACTCCCGCTACATGCAGGAGATGTGTACCAACGTGCTGGAGCGGAttctcac                              >  8:350857/1‑131 (MQ=255)
                                                                                                            aaTACCGTGATCCTCGCGCCGGAGTCGCTGTGGA‑TTACCGGGTTTTGGGTTTACATACAATAATTGTCTGATCAGGGCCGGAACAACTACCCCGACTGGCTGGCGTTATGGGCCCACGGGCGGCAGCCGCTGGTAAc                              >  2:376328/1‑137 (MQ=255)
                                                                                                            aaTACCGTGATCCTCGCGCCGGAGTCGCGTTGGT‑TTAACGGGGTGTGGGGTTTAATACCCTACATGTGTGATAAGGGCCTGAACAACACCCCCCACTGGCGGGCGTGGTGGTCCCAGTTGCGTCACCCGCTgggtaa                              >  6:251759/1‑133 (MQ=255)
                                                                                                            aaTACCGTGATCCTCGCGCCGGAGTCGCGTTGGA‑TTACCGGGTTGTGGGATTACATCCAAAACATGTCTGCACAGGGCCTGAACAACTCCCGCTACTGGCTGGCGGTCTGGCCCAAGGTGCTGCAGCCGCTGGTaaa                              >  8:99911/1‑136 (MQ=255)
                                                                                                            aaTACCGTGATCCTCGCGCCGGAGTCGCGTTCGA‑TTTACGGGTTGTTGGTTTTCATACACAACTTGTGTGATCAGGGCCTGAACAAATACCCCTACTGGCTGGCGGTCTGGACCAAGGTGCTGCAGCCGCTGGTaaa                              >  4:79554/1‑136 (MQ=255)
                                                                                                            aaTACCGTGATCCTCGCGCCGGAGTCGCGGTCGA‑TTACCCGGTTGTGGGATTAGATCCCCGAATTTTCGGAAAACGGCCAGAACAACACCCCCAACTGGCAGGCGGGCTGGACCCAGGTGCTGCAGCCGAGGGTAAc                              >  6:161756/1‑137 (MQ=255)
                                                                                                              tACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGTATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGTGCTGCAGCCGATGGTAAcgg                            <  6:103778/137‑1 (MQ=255)
                                                                                                                ccGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGTGCTGCAGCCGATGGTAAcggcg                          <  3:214256/137‑1 (MQ=255)
                                                                                                                ccGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGTGCTGCAGCCGATGGTAAcggcg                          <  1:439907/137‑1 (MQ=255)
                                                                                                                 cGTGATCCTCGCGCCGGAGTCGCTTTGGT‑TTTACGGGTTTTGGGTTTTAATCAAAGAATTTTATGTTAATGGCCGGAAAAACACCCGCGACTGGCTTGGGTTGTTGGCCAAGGTGCTGAAGACGATGGTTAaggcgg                         >  4:398/1‑137 (MQ=255)
                                                                                                                 cGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTTCCGGGTTTTTGGATTACATAAAATAATTGTATGATAAGGGGCTGCACAAAAACCGCAACATGCGGGCGTTGTTGACCAAGGTGCTGCACCCGATGGTAAaggcgg                         >  8:52909/1‑137 (MQ=255)
                                                                                                                 cGTGATCCTCGCGCCGGAGTCGCGTTCGA‑TTAACGGGTTGTGGGATTAAAAACAAAAATTTTCTGAAAAAGGCCTGAAAAAAAACCCCAACAGGCTGGCCTTCAGGACCAAGGTGCTGCAGCCGATGGTAAacgcgg                         >  4:202912/1‑137 (MQ=255)
                                                                                                                      tCCTCGCGCCGGAGTCGCTTTCGA‑TTAACCGGTTGTGGGGTTACATCCACTACATGTCTGATAAGGGCCTGAACAACAACCGCTACAGGATGGCGGTATGGACCAACGTGCTGCAGCCCATGGTAACCGCGGcccag                    >  6:427336/1‑135 (MQ=255)
                                                                                                                      tCCTCGCGCCGGAGTCGCGTTGGATTTGCCGGGTT‑TGGGGTTTCATACCCGAATTGTGTGTTCACGGGCGGGACAAAGCCCCCGACTGGCTGGGGGTGTGGGCCAAGGTGCTGCAGCCGCTGGTAACCGGGGcccgg                    >  4:38810/1‑135 (MQ=255)
                                                                                                                      tCCTCGCGCCGGAGTCGCGTTGGA‑TTACCGGGTTGTGGGCTTACATCCACGACTTGTCTGATCAGGGCCTGAACAACTCCCGCGACTGGCTGGCGGTCTGGCCCAAGGTGCTGCCGCCGCTGGTAACGGCGGcccgg                    >  2:242794/1‑135 (MQ=255)
                                                                                                                      tCCTCGCGCCGGAGTCGCGTTGGA‑TTACCGGGGTGTGGGGTTTCATCCACTACTTGTGGGGTGAGGGGCTGAACAACTCCCGCGACGGGCGGGCGGTGTGGGCCAAGGTGCTGCCGCCGCTGGTAACGGCGGcccgg                    >  4:364634/1‑135 (MQ=255)
                                                                                                                                  aGTCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGTGCTGCAGCCGATGGTAACGGCGGCCCTGGTGCTGATGGCg        <  8:26137/137‑1 (MQ=255)
                                                                                                                                        tttCGA‑TTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGTGCTGCAGCCGATGGTAACGGCGGCCCTGGTGCTGATGGCGATTTCg  <  3:202912/137‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                         
CACTGGCTGCTCGCCGATGTGCGTACCACCTACTTCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGTGCTGCAGCCGATGGTAACGGCGGCCCTGGTGCTGATGGCGATTTCG  >  ACB122/1115308‑1115580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑