Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,740,4070TG46.5% 4.6 / 15.6 43E53A (GAG→GCG) CJ019_01643hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/23);  new base G (1/19);  total (1/42)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TGTCCACCTTCTCTTGGAAGGCCGGGTGCATTGTTGTCTGCATGGATGATCCTCGGGTGGGTCAGGCGTGGTATTCGAAGGCCTCGGCCTTACGAACGATTCGAACTTGGGC‑GGTGCGCCGCTCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAGGCAGAGCGGAGTGATGATCTTCTGGTATGCCGCCTCTGCTACGAGAGCAGT  >  ACB122/1740285‑1740537
                                                                                                                           |                                                                                                                                  
tgtCCACCTTCTCTTGGAAGGCCGGGTGCATTGTTGTCTGCATGGATGATCCTCGGGTGGGTCAGGCGTGGTATTCGAAGGCCTCGGCCTTACGAACGATTCGAACTTGGGC‑GGTGCGCCGCGCCGGCGCGcggcgg                                                                                                                      <  3:321648/137‑1 (MQ=255)
tgtCCACCTTCTCTTGGAAGGCCGGGTGCATTGTTGTCTGCATGGATGATCCTCGGGTGGGTCAGGCGGGGTATTCGACGGCCTCGGCCTTACGAACGATTCCAACTTGGGC‑GGGGCGCCGCTCCGGCGCGcggcgg                                                                                                                      <  2:251014/137‑1 (MQ=255)
ggtCCACCTTCTCTTGGATGGCCGGGTGCTTTGTTGTCTGAATGTATGATCCTCGGGTGGGTCAGGCGTGGTATTCGAAGGCCTCGGCCTTACGAACGATTCGAACTTGGGC‑GGTGCGCCGCTCGGGCGCGcggcgg                                                                                                                      <  8:129684/136‑1 (MQ=21)
  cccACCTTCTCTTGCAAGGCCGGGTGCTTGGGTGGCGGCATGGCTGATCCTCGGGTGGGGCAGGCGTGGTATTCGAAGGCCGCGGCCTTACGAACGATTCGAACTTGGGC‑GGGGCGCCGCTCCGGCGCGCGGCGGTc                                                                                                                    <  2:108765/136‑1 (MQ=18)
                         gTGCATTGTTGTCTGCATGGATGATCCTCGGGTGGGTCAGGCGTGGTATTCGAAGGCCTCGGCCTTACGAACGATTCGAACTTGGGC‑GGTGGGCCGCGCCGGCCCCCCGCGGGCGCGCCGCCAGGGGgcgcaggggg                                                                                             >  8:198876/1‑131 (MQ=17)
                           gCATTGTTGGCTGCATGGATGATCCTCGGGGGGGGCAGGCGTGGTATTCGAAGGCCTCGGCCTTACTAACGATTCGAACTTGGGC‑GGTGCGCCGCTCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTg                                                                                           <  6:120966/137‑1 (MQ=255)
                                      tccATGGATGATCCTCGGGTGGGTCAGGCGTGGTATTCGAAGGCCTCGGCCTTACGAACGATTCGAACTTGGGC‑GGTGCGCCGCGCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGgcatg                                                                                <  4:411378/135‑1 (MQ=255)
                                      tGCTTGGATGATCGTCGGGGGGGGCAGGCGTGGTATTCGAAGGCCTCGGCCTTACGAACGATTCGAACTTGGGC‑GGTGCGCCGCTCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGgcatg                                                                                <  6:431294/137‑1 (MQ=255)
                                      tGCATGGATGATCCTGGGGTGGGTCAGCGGGTGTATGCGAAGGCCGCGGCCTTACGATCGATTCTAACTTGGGC‑GGTGCGCCGCGCCGGCGCGCGGAGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGgcatg                                                                                <  8:34019/137‑1 (MQ=18)
                                      tGCATGGATGATCCTCGGGTGGGGCAGGCGGGGTATTCGAAGGCCTCGGCCTTACGAACGATTCGAACTTGGGC‑GGTGCGCCGCTCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGgcatg                                                                                <  8:99321/137‑1 (MQ=255)
                                      ggcttGGATGACCCTGGGGTGGGTCTGGCGGTGTATTCGACGGCCTGGGCGTTACGAACGATTCGAAGTTGGGC‑GGTGCGCCGCGCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGgcatg                                                                                <  2:405521/133‑1 (MQ=17)
                                           ggattctCCTCGGGTGGGTCAGGCGTGGTATTCGAAGGCCTCGGCCTTACGAACGATTCGAACTTGGGC‑GGTGCGCCGCTCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTg                                                                           <  1:250924/131‑1 (MQ=255)
                                           ggaggaTCCTGGGGGGGGTCAGGCGTGGTATTCGAAGGCCTCGGCCTTACGAACGATTCTAACTTGGGC‑GGTGCGCCGCTCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTg                                                                           <  1:32192/137‑1 (MQ=255)
                                           ggATGATCCTCGGTGGGGTCAGGCGTGGTATTCGAAGGCCTCGGCCTTACGAACGATTCGAACTTGGGC‑GGTGCGCCGCTCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTg                                                                           <  8:18768/137‑1 (MQ=255)
                                           ggATGATCCTCGGGTGGGTCAGGCGTGGTATTCGAAGGCCTCGGCCTTACGAACGATTCGAACTTGGGC‑GGTGCGCCGCTCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTg                                                                           <  6:87340/137‑1 (MQ=255)
                                           ggATGATCCGCGGGTGGGTCAGGCGTGGTATTCGAAGGCCTCGGCCTTACGAACGATTCGAACTTGGGC‑GGTGCGCCGCGCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTg                                                                           <  7:8363/137‑1 (MQ=255)
                                                ctCCTGGGGTGGGTCAGGCGTGGTATTCGACGGCCTCGGCCTTACGAACGATTCGAACTTGGGC‑GGTGCGCCGCGCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCcaca                                                                      <  4:221002/136‑1 (MQ=255)
                                                cgcgtCGGGGGGGTCCGCCGTGGTATACGAAGGCCACGGCCTTACGAACGATTCGACCTTGGGC‑GGGGCGCCGCGCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCcaca                                                                      <  6:370425/133‑1 (MQ=17)
                                                     ggggtgggtCAGGCGTGTTATTCGAAGGCCTCGGCCTTACGAACTATTCGAACTTGGGC‑GGGGCGCCGCTCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAagaca                                                                 <  1:277326/136‑1 (MQ=255)
                                                     ggggtgggtCAGGCGTGGTATTCGAAGGCCTCGGCCTTACGAACGATTCGAACTTGGGC‑GGTGCGCCGCTCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAagaca                                                                 <  7:99285/136‑1 (MQ=255)
                                                     ggggggggtCTGGCGTGGTATTCGAAGGCCTCGGCCTTACGAACGATTCGAACTTGGGC‑GGGGCGCCGCGCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAagaca                                                                 <  7:53580/136‑1 (MQ=255)
                                                          gggtCAGGCGTGGTATGCGACGGCCTCGGCCTTACTAACTATTCCAACTTGGGC‑GGGGCGCCGCGCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCa                                                            <  2:183356/137‑1 (MQ=18)
                                                            ttCAGGGGTGGATTTCGAAGGCCTCGGCCTAACGATCTATTCCAAGTTGGGC‑GGTGCGCCGCTCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAgg                                                          <  7:378999/136‑1 (MQ=21)
                                                            ggctggCGTTGTATTCGAAGGCCTCGGCCTTACGAACGATGCGACCTTGGGC‑GGGGCGCCGCGCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAgg                                                          <  2:350478/133‑1 (MQ=21)
                                                               cggCGTGGTATTCGAAGGCCTCGGCCTTACGAACGATTCGAACTTGGGC‑GGTGCGCCGCTCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCg                                                       <  5:394889/136‑1 (MQ=255)
                                                               cggCGTGGTATTCGAAGGCCTCGCCATAACCAACGATGCAAACTGGGGCGGGTGC‑CGGCGCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCg                                                       <  2:404201/136‑1 (MQ=21)
                                                                    gggTATTCGAAGGCCTCGGCCTTACGAACGATTCGACCTTGGGC‑GGGGCGCCGCGCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAGGCa                                                  <  3:158338/136‑1 (MQ=255)
                                                                      ttATTGGAAGGCCTGGGCCTTACGAACGATTCGAAGTTGGGG‑GGGGCGCCGCGCGGGCGCGGGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAGGCaga                                                <  3:57573/136‑1 (MQ=25)
                                                                            gAAGGCCTCGGCCTTACGAACTATTCGATCTGGGGC‑GGTGCGCCGCTCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAGGCAGAGCGGAg                                          <  6:320933/137‑1 (MQ=255)
                                                                            gAAGGCCTCGGCCTTACGAACGATTCGAACTTGGGC‑GGTGCGCCGCTCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAGGCAGAGCGGAg                                          <  6:113947/137‑1 (MQ=255)
                                                                            gAAGGCCTCGGCCTTACGAACGATTCGAACTTGGGC‑GGTGCGCCGCGCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAGGCAGAGCGGAg                                          <  3:148262/137‑1 (MQ=255)
                                                                              gggacgcGGCCTTACGAACTATTCGAACTTGGGC‑GGGGCGCCGCTCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAGGCAGAGCGGAGtg                                        <  3:138577/131‑1 (MQ=255)
                                                                              cggCCTGGGACTTACGAACGATGCGAATTTGGGC‑GGTGCGCCGCTCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAGGCAGAGCGGAGtg                                        <  7:25104/136‑1 (MQ=255)
                                                                              cggCCGCGGCCTTACTAACTATTCGACCTTGGGC‑GGGCCGCCGCTCGGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAGGCAGAGCGGAGtg                                        <  2:403656/136‑1 (MQ=25)
                                                                              aGGCCTCGGCCTTACGAACGATTCGAACTTGGGC‑GGTGGGCAGCTCGGGCGCGCGGCGGTGGCGGCGCAGGGGGTCGCTGTCGTTGATGGCGGGATGCATTGCCACAAGACAGACCAGGGCGAGGCAGAGCGGAGtg                                        <  2:274095/137‑1 (MQ=25)
                                                                                 ccTCGGCCTTACGAACGATTCGAACTTGGGC‑GGTGCGCCGCTCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAGGCAGAGCGGAGtgatg                                     <  5:102048/137‑1 (MQ=255)
                                                                                 ccTCGGCCTTACGAACGATTCGAACTTGGGC‑GGTGCGCCGCTCCGGCGCGCGGCGGTCGCGGCGCATGGGGGCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAGGCAGAGCGGAGtgatg                                     <  8:339808/137‑1 (MQ=255)
                                                                                    cGGCCTTACGAACGATGCTAACTTGGGC‑GGTGCGCCGCGCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAGGCAGAGCGGAGTGATGAtc                                  <  4:414774/137‑1 (MQ=255)
                                                                                                cgaTTCGAACTTGGGC‑GGGGCGCCGCGCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAGGCAGAGCGGAGTGATGATCTTCTGGTATgcc                      <  2:141083/137‑1 (MQ=255)
                                                                                                 tatccGAAGTGGGGC‑GGGCCGCCGCGCCGGCGCGCGGCGGGCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAGGCAGAGCGGAGTGATGATCTTCTGGTATgccg                     <  1:342149/133‑1 (MQ=38)
                                                                                                           ggggC‑GGTGCGCCGCTCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAGGCAGAGCGGAGTGATGATCTTCTGGTATGCCGCCTCTGCTAc           <  2:308304/136‑1 (MQ=255)
                                                                                                             ggC‑GGGGCGCCGCGCCGGCGCGCGGCGGGCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAGGCAGAGCGGAGTGATGATCTTCTGGTATGCCGCCTCTGCTACga         <  2:384177/137‑1 (MQ=255)
                                                                                                              ggcgtggcgcCGCGCGGGCGCGCGGCGGGCGCGGCGAATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAGGCAGAGCGGAGTGATGATCTTCTGGTATGCCGCCTCTGCTACga         <  6:235683/131‑1 (MQ=255)
                                                                                                                     cgccgcgccgGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAGGCAGAGCGGAGTGATGATCTTCTGGTATGCCGCCTCTGCTACGAGAGCAGt  <  5:415964/137‑1 (MQ=255)
                                                                                                                     cgccgcgccgGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAGGCAGAGCGGAGTGATGATCTTCTGGTATGCCGCCTCTGCTACGAGAGCAGt  <  5:140874/137‑1 (MQ=255)
                                                                                                                           |                                                                                                                                  
TGTCCACCTTCTCTTGGAAGGCCGGGTGCATTGTTGTCTGCATGGATGATCCTCGGGTGGGTCAGGCGTGGTATTCGAAGGCCTCGGCCTTACGAACGATTCGAACTTGGGC‑GGTGCGCCGCTCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAGGCAGAGCGGAGTGATGATCTTCTGGTATGCCGCCTCTGCTACGAGAGCAGT  >  ACB122/1740285‑1740537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑