Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1222,970,0360GC31.2% 63.9 / 15.5 48intergenic (+99/+50)CJ019_02800/exuThypothetical protein/Hexuronate transporter
Reads supporting (aligned to +/- strand):  ref base G (22/11);  new base C (0/15);  total (22/26)
Fisher's exact test for biased strand distribution p-value = 7.22e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGCTGGAGAAAGCCCAGGCGGTATTGGCCGCGGTCTGATCGAGCGAACGTGGCGCATGGCACCGGTTACGCCACGTTCGCGAGTGAACCCACACCGAACGCTGCATTGTCGTTGTCCGCTTCTCAAGAAACTCCAAGCCAAAAAAA‑GGGCCCGCAA‑GGGCCCCGTAGAGGTAAGTGGCTAGCGCTCTCAGGCGCTCTCGACCTTGCGCGGTGCCATGAAATACATCCACACCAACGCCAGGAAGTACATGGCCGGGATCATGGTAAACA  >  ACB122/2969900‑2970168
                                                                                                                                        |                                                                                                                                      
cgcTGGAGAAAGCCCAGGCGGTATTGGCCGCGGTCTGATCGAGCGAACGTGGCGCATGGCACCGGTTACGCCACGTTCGCGAGTGAACCCACACCGAACGCTGCATTGTCGTTGTCCGCTTCTCAAGAAACTCCAAg                                                                                                                                        >  4:307850/1‑137 (MQ=255)
   tGGAGAAAGCCCAGGCGGTATTGGCCGCGGTCTGATCGAGCGAACGTGGCGCATGGCACCGGTTACGCCACGTTCGCGAGTGAACCCACACCGAACGCTGCATTGTCGTTGTCCGCTTCTCAAGAAACTCCAAGCCa                                                                                                                                     >  2:266562/1‑137 (MQ=255)
      agaAAGCCCAGGCGGTATTGGCCGCGGTCTGATCGAGCGAACGTGGCGCATGGCACCGGTTACGCCACGTTCGCGAGTGAACCCACACCGAACGCTGCATTGTCGTTGTCCGCTTCTCAAGAAACTCCAAGCCaaaa                                                                                                                                  >  2:187828/1‑137 (MQ=255)
        aaaGCCCAGGCGGTATTGGCCGCGTTCTGATCGAGCGAACGTGGTGCATGGCACCGGTTACGCCACGTTCGCTAGTGAACCCACACCGAACGCTGCATTGTCGTTGTCCGCTTCTCAAGAAACTCCAAGCCaaaaaa                                                                                                                                <  3:287453/137‑1 (MQ=255)
         aaGCCCAGGCGGTATTGGCCGCGGTCTGATCGAGCGAACGTGGCGCATGGCACCGGTTACGCCACGTTCGCGAGTGAACCCACACCGAACGCTGCATTGTCGTTGTCCGCTTCTCAAGAAACTCCAAGCCaaaaaaa                                                                                                                               <  5:421546/137‑1 (MQ=255)
             ccAGGCGGTATTGGCCGCGGTCTGATCGAGCGAACGTGGCGCATGGCACCGGTTACGCCACGTTCGCGAGTGAACCCACCCCGAACGCTGCATTGTCGTTGTCCGCTTCTCAAGAAACTCCAAGCCAAAAAAA‑GGGc                                                                                                                          >  2:424871/1‑137 (MQ=255)
             ccAGGCGGTATTGGCCGCGGTCTGATCGAGCGAACGTGGCGCATGGCACCGGTTACGCCACGTTCGCGAGTGAACCCACACCGAACGCTGCATTGTCGTTGTCCGCTTCTCAAGAAACTCCAAGCCAAAAAAA‑GGGc                                                                                                                          >  7:142940/1‑137 (MQ=255)
                  cGGTATTGGCCGCGGTCTGATCGAGCGAACGTGGCGCATGGCACCGGTTACGCCACGTTCGCGAGTGAACCCACACCGAACGCTGCATTGTCGTTGTCCGCTTCTCAAGAAACTCCAAGCCAAAAAAA‑GGGCCCGCa                                                                                                                     >  7:112417/1‑137 (MQ=255)
                  cGGTATTGGCCGCGGTCTGATCGAGCGAACGTGGCGCATGGCACCGGTTACGCCACGTTCGCGAGTGAACCCACACCGAACGCTGCATTGTCGTTGTCCGCTTCTCAAGAAACTCCAAGCCAAAAAAA‑GGGCCCGCa                                                                                                                     >  5:430646/1‑137 (MQ=255)
                  cGGTATTGGCCGCGGTCTGATCGAGCGAACGTGGCGCATGGCACCGGTTACGCCACGTTCGCGAGTGAACCCACACCGAACGCTGCATTGTCGTTGTCCGCTTCTCAAGAAACTCCAAGCCAAAAAAA‑GGGCCCGCa                                                                                                                     >  5:104272/1‑137 (MQ=255)
                              cgGTCTGATCGAGCGAACGTGGCGCATGGCACCGGTTACGCCACGTTCGCGAGTGAACCCACACCGAACGCTGCATTGTCGTTGTCCGCTTCTCAAGAAACTCCAAGCCAAAAAAA‑GGGCCCGCAA‑GGGCCCCGTag                                                                                                        >  5:146944/1‑137 (MQ=255)
                                  cTGATCGAGCGAACGTGGCGCATGGCACCGGTTACGCCACGTTCGCGAGTGAACCCACACCGAACGCTGCATTGTCGTTGTCCGCTTCTCAAGAAACTCCCAGCCAAAAAAA‑GGGCCCGCAA‑GGGCCCCGTAGAGGt                                                                                                    >  3:104522/1‑137 (MQ=255)
                                  cTGATCGAGCGAACGTGGCGCATGGCACCGGTTACGCCACGTTCGCGAGTGAACCCACACCGAACGCTGCATTGTCGTTGTCCGCTTCTCAAGAAACTCCAAGCCAAAAAAA‑GGGCCCGCAA‑GGGCCCCGTAGAGGt                                                                                                    >  1:296483/1‑137 (MQ=255)
                                  cTGATCGAGCGAACGTGGCGCATGGCACCGGTTACGCCACGTTCGCGAGTGAACCCACACCGAACGCTGCATTGTCGTTGTCCGCTTCTCAAGAAACTCCAAGCCAAAAAAA‑GGGCCCGCAA‑GGGCCCCGTAGAGGt                                                                                                    >  7:441313/1‑137 (MQ=255)
                                  cTGATCGAGCGAACGTGGCGCATGGCACCGGTTACGCCACGTTCGCGAGTGAACCCACACCGAACGCTGCATTGTCGTTGTCCGCTTCTCAAGAAACTCCAAGCCAAAAAAA‑GGGCCCGCAA‑GGGCCCCGTAGAGGt                                                                                                    >  5:219177/1‑137 (MQ=255)
                                  cTGATCGAGCGAACGTGGCGCATGGCACCGGTTACGCCACGTTCGCGAGTGAACCCACACCGAACGCGGCATTGTCGTTGTCCGCTACTCAAGAAACTACAAGCAAAAAAAA‑AGGCCCGCAA‑GGGCCCCCTAGAGGt                                                                                                    >  8:385601/1‑137 (MQ=255)
                                  cTGATCGAGCGAACGTGGCGCATGGCACCGGTTACGCCACGTTCGCGAGTCAACCCACACCGAACGCTGCATTGTCGTTGTCCGCTTCTCAACAAACTCCAAGCCAAAAAAA‑GGGCCCGCAA‑GGGCCCCGTAGAGGt                                                                                                    >  8:365411/1‑137 (MQ=255)
                                        gAGCGACCGTGGCGCCGGGCACGGGTTCCGCCACGTTGGCTTGTGACCCCACACCGACCGCTCCATTGTCGTTGCCCGCTTCTCACCAAACTCCAACCAAAAAAAC‑GGCCCCGCAC‑GGGCCCCGTAGAGGTAAGTgg                                                                                              <  6:397785/137‑1 (MQ=255)
                                         accGAACGTGGCGCATGCACCCGTTTACCCCACTTTCGCTTTTTACCCCAAACCCAACGCTGAATTTTCTTTGTCCGATTCTAAAGAAACTCAAACCCAAAAAAA‑GGGCCCGCAA‑GGGCCCCGTAGAGGTAAGTGGc                                                                                             <  6:187638/135‑1 (MQ=255)
                                              aCGTGGCGCATGGCACCGGTTACGCCACGTTCGCGAGTGAACCCACACCGAACGCTGCATTGTCGTTGTCCGCTTCTCAAGAAACTCCAAGCCAAAAAAA‑GGGCCCGCAA‑GGGCCCCGTAGAGGTAAGTGGCTAgcg                                                                                        >  7:288600/1‑137 (MQ=255)
                                                   gcccagggCACGGGTTCCGCCACGTGCGCGTGTTACCCCACACCGACCGCGGCTTTGGCGTTGTCCGCTTCTAAACAACCCCCACCCCAAAAAAC‑GGGCCCGCAC‑GGGCCCCGTAGAGGTAAGTGGCTAGCGCtctc                                                                                   <  4:293384/131‑1 (MQ=255)
                                                         ggCACCGGTTACGCCACGTTCGCGAGTGAACCCACACCGAACGCTGCATTGTCGTTGTCCGCTTCTCAAGAAACTCCAAGCCAAAAAAA‑GGGCCCGCAA‑GGGCCCCGTAGAGGTAAGTGGCTAGCGCTCTCAGgcgc                                                                             <  8:441313/137‑1 (MQ=255)
                                                         ggCACCGGGTACGCCACGTTCGCGAGTGAACCCACACCGAACGCTGCATTGGCGTTGTCCGCTTCTCAACAAACTCCAAGCCAAAAAAA‑GGGCCCGCAA‑GGGCCCCGTAGAGGTAAGTGGCTAGCGCTCTCAGgcgc                                                                             <  7:333328/137‑1 (MQ=255)
                                                                   accccACGTTCGCGAGTGAACCCACCCCGACCGGTGCTTTGTCGTTGTCCGCTTCTCAAGAAACCCCACCCAAAAAAAC‑GGGCCCGCAC‑GGGCCCCGTAGAGGTAAGTGGCTAGCGCTCTCAGGCGCTCTCGACCtt                                                                   <  6:219177/134‑1 (MQ=255)
                                                                   aCGCCACGTTCGCTAGTGAACCCACACCGAACGCTGCATTGTCGTTGTCCGCTTCTAAAAAAACACCAAGCCAAAAAAA‑GGGCCCGCAA‑GGGCCCCGTAGAGGTAAGTGGCTAGCGCTCTCAGGCGCTCTCGACCtt                                                                   <  4:122010/137‑1 (MQ=255)
                                                                   aCGCCACGTTCGCGAGTGAACCCACACCGAACGCTGCATTGTCGTTGTCCGCTTCTCAAGAAACTCCAAGCCAAAAAAA‑GGGCCCGCAA‑GGGCCCCGTAGAGGTAAGTGGCTAGCGCTCTCAGGCGCTCTCGACCtt                                                                   >  6:247430/1‑137 (MQ=255)
                                                                    ccccACGTGCGCGAGTGAACCCACCCCCACCGCTGCTTTGGCGTTGTCCGCTCCTCAACAAACACCACCCAAAAAAAA‑GGCCCCGCAC‑GGGCCCCGTAGAGGTAAGTGGCTAGCGCTCTCAGGCGCTCTCGACCTTg                                                                  <  7:193224/135‑1 (MQ=255)
                                                                      ccACGTTCGCGAGTGAACCCACACCGAACGCTGCATTGTCGTTGTCCGCTTCTCAAGAAACTCCAAGCCAAAAAAA‑GGGCCCGCAA‑GGGCCCCGTAGAGGTAAGTGGCTAGCGCTCTCAGGCGCTCTCGACCTTgcg                                                                >  2:50663/1‑137 (MQ=255)
                                                                        aCGTTCGCGAGTGAACCCACACCGAACGCTGCATTGTCGTTGTCCGCTTCTCAAGAAACTCCAAGCCAAAAAAA‑GGGCCCGCAA‑GGGCCCCGTAGAGGTAAGTGGCTAGCGCTCTCAGGCGCTCTCGACCTTGcgcg                                                              >  8:351756/1‑137 (MQ=255)
                                                                           ttCGCGAGTGAACCCCCACCTAACGCTGCATTGTCTTTGTCCGCTTCTCAACAACCTCCAACCCAAAAAAC‑GGCCCCGCAC‑GGGCCCCGTAGAGGTAAGTGGCTAGCGCTCTCAGGCGCTCTCGACCTTGCGCGGTg                                                           <  8:216097/137‑1 (MQ=255)
                                                                            gcgcgAGGTAACCCACACCGAACGGTGCATTGTCGTTGTCCGCTTCTAAAGAAACTCCAACCCAAAAAAC‑GGGCCCGCAA‑GGGCCCCGTAGAGGTAAGTGGCTAGCGCTCTCAGGCGCTCTCGACCTTGCGCGGTGc                                                          <  4:286603/136‑1 (MQ=255)
                                                                              gcgAGTCACCCCACACCTAACGCTGCTTTGGCGTGGCCCGCTTCTCAAGAAAATCAAACCAAAAAAAC‑GGGCCCCCAC‑GGGCCCCGTAGAGGTAAGTGGCTAGCGCTCTCAGGCGCTCTCGACCTTGCGCGGTGCCa                                                        <  3:307850/137‑1 (MQ=255)
                                                                                      aCCCACACCCAACGCTGCATTGGCTTTGGCCGCTGCTCAACAAACACCACCCAAAAAAAC‑GGCCCCGCAC‑GGGCCCCGTAGAGGTAAGTGGCTAGCGCTCTCAGGCGCTCTCGACCTTGCGCGGTGCCATGAAATAc                                                <  1:266562/137‑1 (MQ=255)
                                                                                            cccGAACGCTGAATTGTCGTTGTCCGCTTCTCACCAAACACCAACCCAAAAAAA‑GGGCCCGCAA‑GGGCCCCGTAGAGGTAAGTGGCTAGCGCTCTCAGGCGCTCTCGACCTTGCGCGGTGCCATGAAATACATCcac                                          <  8:400045/136‑1 (MQ=255)
                                                                                                  cGCTTCATTGTCGTTGTCCGATTCTCAATAAACTCCAAGCCAAAAAAA‑GGGCCCGCAA‑GGGCCCCGTAGAGGTAAGTGGCTAGCGCTCTCAGGCGCTCTCGACCTTGCGCGGTGCCATGAAATACATCCACACCAAc                                    <  8:44925/137‑1 (MQ=255)
                                                                                                     tgaaTTGTGTTTGGCCGCTTCTAAACAAAC‑ACAACCCAAAAAAACGGGCCCGCAC‑GGGCCCCGTAGAGGTAAGTGGCTAGCGCTCTCAGGCGCTCTCGACCTTGCGCGGTGCCATGAAATACATCCACACCAACGcc                                 <  2:382500/134‑1 (MQ=255)
                                                                                                     ggctttGGCGTTGTCCGCTTCTCAATAAACACCAACCCAAAAAAA‑GGGCCCGCAA‑GGGCCCCGTAGAGGTAAGTGGCTAGCGCTCTCAGGCGCTCTCGACCTTGCGCGGTGCCATGAAATACATCCACACCAACGcc                                 <  7:216052/133‑1 (MQ=255)
                                                                                                     ggattggTCGGTGGCCGCTTCAAAATAACCTCC‑ACCCAAAAAAC‑GGGCCCGCAAGGGGCCCCGTAGAGGTAAGTGGCTAGCGCTCTCAGGCGCTCTCGACCTTGCGCGGTGCCATGAAATACATCCACACCAACGcc                                 <  1:319429/131‑1 (MQ=255)
                                                                                                         ttGTCGTTGTCCGCTTCTCAAGAAACTCCAAGCCAAAAAAA‑GGGCCCGCAA‑GGGCCCCGTAGAGGTAAGTGGCTAGCGCTCTCAGGCGCTCTCGACCTTGCGCGGTGCCATGAAATACATCCACACCAACGCCAGGa                             >  8:50543/1‑137 (MQ=255)
                                                                                                                    cGCTTCTCAAGAAAATCCAAGCCAAAAAAA‑GGGCCCGCAA‑GGGCCCCGTAGAGGTAAGTGGCTAGCGCTATCAGGCGCTCTCGCCCTTGCGCGGTGCCATGAAATAAATCCACAACAACGCCAGGAAGTACATTGcc                  >  4:199853/1‑137 (MQ=255)
                                                                                                                     gCTTCTAAACAAACTCCACGCCAAAAACA‑GGGCCCGCAA‑GGGCCCCGTAGAGGTAAGTGGCTAGCGCTCTCAGGCGCTCTCGACCTTGCGCGGTGCCATGAAATACATCCACACCAACGCCAGGAAGTACATGGCCg                 <  6:236538/137‑1 (MQ=255)
                                                                                                                     gCTTATCATAAAACACCAACACAAAATAC‑GGGCCCGCAC‑GGGCCCCGTAGAGGTAAGTGGCTAGCGCTCTCAGGCGCTCTCGACCTTGCGCGGTGCCATGAAATACATCCACACCAACGCCAGGAAGTACATGGCCg                 <  3:319616/137‑1 (MQ=255)
                                                                                                                       ttCTCAAGAAACTCCAAGCCAAAAAAA‑GGGCCCGCAA‑GGGCCCCGTAGAGGTAAGTGGCTAGCGCTCTCAGGCGCTCTCGACCTTGCGCGGTGCCATGAAATACATCCACACCAACGCCAGGAAGTACATGGCCggg               >  8:367635/1‑137 (MQ=255)
                                                                                                                       ttCTCAAGAAACTCCAAGCCAAAAAAA‑GGGCCCGCAA‑GGGCCCCGTAGAGGTAAGTGGCTAGCGCTCTCAGGCGCTCTCGACCTTGCGCGGTGCCATGAAATACATCCACACCAACGCCAGGAAGTACATGGCCggg               >  8:426180/1‑137 (MQ=255)
                                                                                                                          tcAAGAAACTCCAAGCCAAAAAAA‑GGGCCCGCAA‑GGGCCCCGTAGAGGTAAGTGGCTAGCGCTCTCAGGCGCTCTCGACCTTGCGCGGTGCCATGAAATACATCCACACCAACGCCAGGAAGTACATGGCCGGGATc            <  6:430646/137‑1 (MQ=255)
                                                                                                                          acAAGAAACTCAAACCCAAAAAAC‑GGGCCCGCAC‑GGGCCCCGTAGAGGTAAGTGGCTAGCGCTCTCAGGCGCTCTCGACCTTGCGCGGTGCCATGAAATACATCCACACCAACGCCAGGAAGTACATGGCCGGGATc            <  8:112417/136‑1 (MQ=255)
                                                                                                                                  cTCCAAGCCAAAAAAA‑GGGCCCGCAA‑GGGCCCCGTAGAGGTAAGTGGCTAGCGCTCTCAGGCGCTCTCGACCTTGCGCGGTGCCATGAAATACATCCACACCAACGCCAGGAAGTACATGGCCGGGATCATGGTaaa    <  2:240809/137‑1 (MQ=255)
                                                                                                                                    ccAAGCCAAAAAAC‑GGGCCCGCAA‑GGGCCCCGTAGAGGTAAGTGGCTAGCGCTCTCAGGCGCTCTCGACCTTGCGCGGTGCCATGAAATACATCCACACCAACGCCAGGAAGTACATGGCCGGGATCATGGTAAACa  <  4:271106/137‑1 (MQ=255)
                                                                                                                                    ccAAGCCAAAAAAA‑GGGCCCGCAA‑GGGCCCCGTAGAGGTAAGTGGCTAGCGCTCTCAGGCGCTCTCGACCTTGCGCGGTGCCATGAAATACATCCACACCAACGCCAGGAAGTACATGGCCGGGATCATGGTAAACa  <  2:296483/137‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                      
CGCTGGAGAAAGCCCAGGCGGTATTGGCCGCGGTCTGATCGAGCGAACGTGGCGCATGGCACCGGTTACGCCACGTTCGCGAGTGAACCCACACCGAACGCTGCATTGTCGTTGTCCGCTTCTCAAGAAACTCCAAGCCAAAAAAA‑GGGCCCGCAA‑GGGCCCCGTAGAGGTAAGTGGCTAGCGCTCTCAGGCGCTCTCGACCTTGCGCGGTGCCATGAAATACATCCACACCAACGCCAGGAAGTACATGGCCGGGATCATGGTAAACA  >  ACB122/2969900‑2970168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑