Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,875,8780AC40.0% 80.9 / 14.8 70T298P (ACC→CCC) ttgI_2Toluene efflux pump outer membrane protein TtgI
Reads supporting (aligned to +/- strand):  ref base A (10/32);  new base C (27/1);  total (37/33)
Fisher's exact test for biased strand distribution p-value = 5.19e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CCTGACGCGCTCAGCGTAGACCTGTCGCCCAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCACCGCCAACGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAG  >  ACB122/3875743‑3876008
                                                                                                                                       |                                                                                                                                  
ccTGACGCGCTCAGCGTAGACCTGTCGCCCAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCAc                                                                                                                                   <  6:247673/137‑1 (MQ=255)
ccTGACGCGCTCAGCGTAGACCTGGCGCCCACGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGCCCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCAc                                                                                                                                   <  2:233265/137‑1 (MQ=255)
                 aGACCTGTCGCCCCTGGCCCTGCGGGCCATCGCCAAGGCGCGGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCGGAGCGCCAGCTGGCGGCGGCCACCGCCAACGTTGGCGTgg                                                                                                                  <  4:88439/137‑1 (MQ=255)
                   aCCTGTCGCCCAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGCCCACCCCCAACCATGGCGTGGcc                                                                                                                >  3:168394/1‑137 (MQ=255)
                     cTGTCGCCCAAGGCCCTGCCGGCCATCCCCACGGCGCTGCCGGGCGGTGCCCCGGGGGCGCGGCTCCGCCGCCGCCCGCACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCACCGCCAACGTTGGCGTGGCCAc                                                                                                              <  2:275723/137‑1 (MQ=255)
                      tgacGCCCAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCCGGCGCCCCCGCCAACCATGGCCTTGACAcc                                                                                                             >  1:211653/4‑137 (MQ=255)
                      tGTCGCCCAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCCGCGGCCCCCGCCAACGTTGGCGTGGCcccc                                                                                                             >  7:382808/1‑137 (MQ=255)
                         cGCCCAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCACCGCCAACGTTGGCGTGGCCAccgcc                                                                                                          <  6:207051/137‑1 (MQ=255)
                           cccAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCCGCGCCGAGCGCCAGCAGGCGGCGGCCCCCCCCCACCATGGCGGGGCCCcccccgc                                                                                                        >  2:264007/1‑136 (MQ=255)
                           cccAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGGCCCCGCCCACCGTGGCGTGGCCCcccccgc                                                                                                        >  2:158731/1‑136 (MQ=255)
                           cccAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCCCCGCCCACGTTGGCGTGGCCCccgccgc                                                                                                        >  4:179737/1‑136 (MQ=255)
                           cccAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCCCCGCCCACGTTGGCGTGGCCAccgccgc                                                                                                        >  3:332268/1‑136 (MQ=255)
                           cccAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCCCCGCCCACCTTGGCGTGGCCCccgccgc                                                                                                        >  3:382446/1‑136 (MQ=255)
                           cccAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCCCCGCCCACCTTGGCGTGGCCAccgccgc                                                                                                        >  3:365686/1‑136 (MQ=255)
                           cccAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCCCCGCCAACGTTGGCGTGGCCAccgccgc                                                                                                        >  8:198482/1‑136 (MQ=255)
                           cccAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCCCCGCCAACGTTGGCGTGGCCAccgccgc                                                                                                        >  1:141922/1‑136 (MQ=255)
                           cccAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCCCCCCCCACGGTGGCGTGGGCCccgcccc                                                                                                        >  1:202388/1‑135 (MQ=255)
                           cccAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCACCGCCCACGTTGGCGTGGCCCccgccgc                                                                                                        >  8:176460/1‑136 (MQ=255)
                           cccAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCACCGCCCACGTTGGCGTGGCCCccgccgc                                                                                                        >  2:273666/1‑136 (MQ=255)
                           cccAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCACCGCCCACGTTGGCGTGGCCAccgccgc                                                                                                        >  8:134006/1‑136 (MQ=255)
                           cccAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCACCGCCAACGTTGGCGTGGCCAccgccgc                                                                                                        >  6:89317/1‑136 (MQ=255)
                           cccAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCACCGCCAACCTTGGCGTGGCCAccgcccc                                                                                                        >  2:4321/1‑135 (MQ=255)
                           cccAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCACCCCCAACGTTGGCGTGGCCAccgccgc                                                                                                        >  3:151540/1‑136 (MQ=255)
                           cccAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCCGCCCCCGCCCACCTTGGCGTGGCCAccccccc                                                                                                        >  1:138951/1‑135 (MQ=255)
                                ggCCGTGCCGGCCATCGCCACGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCACCGCCAACGTTGGCGTGGCCACCGCCGACCTGt                                                                                                   <  7:430356/137‑1 (MQ=255)
                                  cccTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCCCCGCCCACGTTGGCGTGGCCACCCCCGCCCTGtta                                                                                                 >  8:441426/1‑136 (MQ=255)
                                       ccGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCCCCGCCCACGTTGGCGTGGCCACCGCCGACCAGTTCCcgcc                                                                                            >  3:215714/1‑136 (MQ=255)
                                        cGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGGCCCCGCCCACGTTGGCGTGGGCCCCGCCGACCGGGTCCcgccc                                                                                           >  1:147345/1‑135 (MQ=255)
                                        cGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCCCCGCCCACGATGGCGTGGCCCCCGCCGCCCGGGTCCcccgc                                                                                           >  7:372319/1‑137 (MQ=255)
                                        cGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCCCCGCCAACGTTGGCGTGGCCACCGCCGCCCTGTTCCcgcgc                                                                                           >  7:392422/1‑137 (MQ=255)
                                         ggCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCCCCGCCCACGTTGGCGTGGCCCCCCCCGCCCGGTTCCcccgcc                                                                                          >  2:205941/1‑136 (MQ=255)
                                           ccATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCACCGCCAACGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTc                                                                                        <  3:215329/137‑1 (MQ=255)
                                              gcGCCACGGCGCTGCGGGGCGGTGACCCGGGTGAGCGGCGGCGCCGCCGCCCGGACATCCGCCGCGCCGAGCGCCAGCTGGCGGCGGCCACCGCCAACGTGGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGc                                                                                     <  3:229465/136‑1 (MQ=255)
                                               cGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCCCCGCCAACGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGcc                                                                                    >  6:28466/1‑137 (MQ=255)
                                               cGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCACCGCCAACGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGcc                                                                                    <  7:441426/137‑1 (MQ=255)
                                                         cTGCCGGTCGGTGACCGGGGTGTGCTGCTGCGCCGCCGCCCGGACATCCGCCGCGCCGAGCGCCTGGTGGCGGCGGCCACCGCAAAGGTTGGCGTGGCCACCGCCGACCTGTACCCGCGCGTCAGCCTCAGTGGCtt                                                                          <  3:165656/137‑1 (MQ=255)
                                                          ggCCGGGCGGTGACCCGGGTGAGCGGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCACCGCCAACGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTc                                                                         <  8:444436/136‑1 (MQ=255)
                                                             cggtcggtGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCCCCGCCAACGTTGGCGTGGCCACCGCCGCCCTGTTACCGCGCGTCAGCCGCAGTGGCTTCCTc                                                                      >  6:96195/1‑137 (MQ=255)
                                                             cggtcggtGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCCCCGCCAACGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTc                                                                      >  6:209381/1‑137 (MQ=255)
                                                             cggtcggtGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCCCCGCCAACGTTGGCGTGGCCACCCCCGCCCAGTTCCCGCGCGTCAGCCTCACTGGCGTccac                                                                      >  4:393752/1‑135 (MQ=255)
                                                             cggtcggtGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCCCCCCCCCCCTTGGCGTGGCCCCCGCCGCCCTGTTCCCGCGCGTCACCCTCCGTGGCTTCCTc                                                                      >  4:221802/1‑137 (MQ=255)
                                                             cggtcggtGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCACCGCCAACGTTGGCGTGGCCACCGCCGACCGGGTCCCGCGCGTCAGCCTCAGTGGCTTCCTc                                                                      >  7:237270/1‑137 (MQ=255)
                                                              gggcggtGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCACCGCCAACGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCg                                                                     <  7:173684/134‑1 (MQ=255)
                                                                       cccGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCCCCGCCAACGTTGGCGTGGCCACCCCCGCCCTGTTCCCGCCCGTCAGCCTCAGTGGCTTCCTCGGCATTAccc                                                            >  8:398232/1‑136 (MQ=255)
                                                                           ggggAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCACCGCCAACGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACcgccgc                                                        <  6:369721/134‑1 (MQ=255)
                                                                             tGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCACCGCCAACGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGccgccc                                                      <  6:305670/137‑1 (MQ=255)
                                                                                 ctgctgCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCACCGCCAACGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCgg                                                  >  7:29926/1‑137 (MQ=255)
                                                                                  tgctgcGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCACCGCCAACGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGc                                                 <  7:191154/137‑1 (MQ=255)
                                                                                    ctgcGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCCCCGCCCACGTTGGCGTGGCCACCGCCGCCCGGTTCCCGCGCGTCCGCCGCAGTGGCGTCCTCGGCTTTACCGCCGCCCGCGGCTc                                               >  1:231329/1‑137 (MQ=255)
                                                                                     tgggccgccgccCGGTCATCGGCAGGGCCGAGGGCCTGCTGGCGGCGTACACCGCCATCGTTGGGGTTGCCAGCGCCTACCTGTTCGCGCGGGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCg                                              <  5:419841/134‑1 (MQ=255)
                                                                                                 gggACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCACCGCCAACGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGctct                                  <  4:382446/136‑1 (MQ=255)
                                                                                                         cGCAGCGCCGAGCGCATGGGGGCGGCGGCCACCGCCAACGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCggcggc                          <  3:170371/137‑1 (MQ=255)
                                                                                                               gcCGAGCGCCAGCTGGCGGCGGCCACCGCCAACGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGc                    <  6:323105/137‑1 (MQ=255)
                                                                                                               gcCGAGCGCCAGCTGGCGGCGGCCACCGCCAACGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGc                    <  7:198482/137‑1 (MQ=255)
                                                                                                               gcCGAGCGCCAGCTGGCGGCGGCCACCGCCAACGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGc                    <  2:202388/137‑1 (MQ=255)
                                                                                                               gcCGAGCGCCAGCTGGCGGCGGCCACCGCCAACGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGc                    <  3:393752/137‑1 (MQ=255)
                                                                                                                 cGAGCGCCAGCTGGCGGCGGCCCCCGCCAACGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCGCGCAGATCGGCGCTTCGGCGGCCCACGccg                  >  4:254282/1‑136 (MQ=255)
                                                                                                                 cGAGCGCCAGCTGGCGGCGGCCACAGCCAACCTTGGCGTGGCCACCGCCGCCCTGTTACCGCGCGTCAGCCTCAGTGGGGTACTAGGCTTTACCGCCGCCCGCGGCTCGGAGATCGGCTCTTCGGCGGCCacagccg                  >  5:187231/1‑131 (MQ=255)
                                                                                                                   aGCGCCAGCTGGCGGCGGCCACCGCCAACGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTgg                <  6:336175/137‑1 (MQ=255)
                                                                                                                    gcgcCAGCTGGCGGCGGCCACCGCCAACGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTggg               <  1:5682/137‑1 (MQ=255)
                                                                                                                          gcgggcggcggcCACCGCCAACGTTGGCGGGGCCACCGCCGACCTGTTCCCGCGCGGCAGCCTCAGTGGCATCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTgg         <  1:193912/134‑1 (MQ=255)
                                                                                                                          gCTGGCGGCGGCCACCGCCTACGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTgg         <  7:151200/137‑1 (MQ=255)
                                                                                                                          gCTGGCGGCGGCCACCGCCAACGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTgg         <  5:127081/137‑1 (MQ=255)
                                                                                                                          gCTGGCGGCGGCCACCGCCAACGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTgg         <  5:401845/137‑1 (MQ=255)
                                                                                                                          gCTGGCGGCGGCCACCGCCAACGTGGGCGGGGCCACCGCCGACCTGTTCCCTCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCTCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTgg         <  7:310176/137‑1 (MQ=255)
                                                                                                                           cTGGCGGCGGCCCCCGCCAACGTTGGCGTGGCCCCCGCCGCCCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCGCGGCTTTTCCGCCGCCCGCGGCGCGCCGCTCGGCGCTTCGGCGGCCCACGCCGGGGCCCTggg        >  6:434867/1‑137 (MQ=255)
                                                                                                                           cTGGCGGCGGCCACCGCCAACGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTggg        <  4:373076/137‑1 (MQ=255)
                                                                                                                           cTGGCGGCGGCCACCGCCAACGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTggg        <  8:58827/137‑1 (MQ=255)
                                                                                                                              gcggcggcCCCCGCCAACGTGGGCGGGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGccc     <  1:158731/137‑1 (MQ=255)
                                                                                                                                 gcggcCACCGCCAACGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAg  <  8:135154/137‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                  
CCTGACGCGCTCAGCGTAGACCTGTCGCCCAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGCCACCGCCAACGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAG  >  ACB122/3875743‑3876008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑