Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	4,667,099	0	G	T	25.4%	95.4 / 10.3	63	L161L (CTC→CTA)	chrR	Quinone reductase
Reads supporting (aligned to +/- strand):  ref base G (24/23);  new base T (16/0);  total (40/23)
Fisher's exact test for biased strand distribution p-value = 2.04e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGCGACTGGCGCACCGCATGGTTGGCGCCAAAGCCACCAATCGCCCCCG  >  ACB122/4666971‑4667232                                                                                                                                 |                                                                                                                                      ggggaaaaaCCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTACACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGcc                                                                                                                               <  6:230742/131‑1 (MQ=255)           ccGATCCTACACAGGGGCGTTTCACTCCAGGAATAGTGGTTCAGACCGCCCTGTTCATCTTCACCCATGACGCAAACCTGTCGACATACGCCTTCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGTATCCTCg                                                                                                                     <  2:324195/137‑1 (MQ=255)                  tACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGc                                                                                                              >  5:218605/1‑137 (MQ=255)                     acaGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTcg                                                                                                           <  8:350633/137‑1 (MQ=255)                     acaGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTcg                                                                                                           <  1:46787/137‑1 (MQ=255)                     acaGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTcg                                                                                                           <  6:357081/137‑1 (MQ=255)                          ggCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAACCTCGCCGCa                                                                                                      >  2:256144/1‑137 (MQ=255)                            cGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCACCTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTTCCCGAATCCTCGCACAAGCTCGCAGCAcc                                                                                                    >  8:121633/1‑137 (MQ=255)                            cGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTTCCCGAATCCTCGACCAAGCTCGCCGcccc                                                                                                    >  2:219011/1‑137 (MQ=255)                            cGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCAcc                                                                                                    >  4:317820/1‑137 (MQ=255)                            cGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCAcc                                                                                                    >  4:280922/1‑137 (MQ=255)                            cGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCAcc                                                                                                    >  4:354164/1‑137 (MQ=255)                            cGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCAcc                                                                                                    <  3:205861/137‑1 (MQ=255)                            cGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCAcc                                                                                                    <  8:411024/137‑1 (MQ=255)                            cGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCGTGTGATTTAGCTTGCCCGAATCCTCGCACAAGCTCGCCGcccc                                                                                                    >  5:330401/1‑137 (MQ=255)                            cGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGGCTTGTGATTGAGCTTGCCCGAATGCGAGAACAATCTCGCCGcccc                                                                                                    >  4:350678/1‑137 (MQ=255)                            cGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGAAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTAGCCCCAcc                                                                                                    >  8:223649/1‑137 (MQ=255)                              ttGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGATCTTGACCGAATACTCGAACAAGATCTCCGCACCGc                                                                                                  >  6:79459/1‑137 (MQ=255)                              ttGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGACATTGACCGAAACCACGAAAAACCACGCCCCACCGc                                                                                                  >  1:415115/1‑137 (MQ=255)                               tGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTTAGCTTGGCCGAATCCTCGAACAAGCTCGCCGCACCGcc                                                                                                 >  5:379276/1‑137 (MQ=255)                                gCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTTAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGccc                                                                                                >  4:387741/1‑136 (MQ=255)                                 cacaGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTGGTGTCATTGAGCTTGCACGAATACTCGAACAACCAAGCCGCACCGCCaa                                                                                               >  2:180970/1‑137 (MQ=255)                                 cacaGCAGGATTAGGGGTGCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCaa                                                                                               <  1:279058/137‑1 (MQ=255)                                      cagGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTaa                                                                                          <  6:246464/137‑1 (MQ=255)                                         gATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTTAGCTTGCCCGAATACTCTAACAATCTAGCCGCACCGCCAAGGTGAGcc                                                                                       >  1:405387/1‑137 (MQ=255)                                         gATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGcc                                                                                       >  2:269056/1‑137 (MQ=255)                                         gATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGcc                                                                                       >  2:141591/1‑137 (MQ=255)                                         gATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCAATGCTCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGAAAGcc                                                                                       >  8:207630/1‑137 (MQ=255)                                           ttAGGGGTTCAGACCGGCCTGTTCAACTTCACCCATGACGAAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGATCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTc                                                                                     <  5:443953/137‑1 (MQ=255)                                              ggggTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCggg                                                                                  <  1:256144/137‑1 (MQ=255)                                                 gTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTAATTTAGCTTGGCCGAATACTCGAAAAATCAAGCCGCACCGCCAATGGAAGCCTCGGGcct                                                                               >  3:423206/1‑135 (MQ=255)                                                   tCAGACCGCCCTGTTCAACTTCACCCATGATGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTTAGCTTGCCCGAATACTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCt                                                                             >  8:434746/1‑137 (MQ=255)                                                      gACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCGTTTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAACCCGCGGGCATAtgca                                                                          >  3:234941/1‑137 (MQ=255)                                                      gACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGGCTTGTGATTTAGCTTTGCCGCAAACGCGCACAAACTCGCCGCCCCCCCAAGGTTAGCCGCGGGCCTCtgcg                                                                          >  1:212445/1‑136 (MQ=255)                                                       aCCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTGTTGTCATTTAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCCCCGCCAATGTAAGCCTCGGGCATCtgcaa                                                                         >  2:181239/1‑136 (MQ=255)                                                       aCCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCtgcat                                                                         >  8:368524/1‑137 (MQ=255)                                                              tGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGc                                                                  >  8:424543/1‑137 (MQ=255)                                                              tGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATACTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGc                                                                  >  5:449935/1‑137 (MQ=255)                                                                   aaCTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTTAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTc                                                             >  4:117662/1‑137 (MQ=255)                                                                   aaCTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATACTCGAACAAGCACGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTc                                                             >  3:418373/1‑137 (MQ=255)                                                                        cACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTTAGCTTGCCCGAATACTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGaa                                                        >  3:294274/1‑137 (MQ=255)                                                                        cACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCa                                                                                >  5:314261/1‑113 (MQ=255)                                                                        cACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCa                                                                                <  6:314261/113‑1 (MQ=255)                                                                             aTGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTTAGCTTTCCCGAATCCTAGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACAcc                                                   >  5:118424/1‑137 (MQ=255)                                                                             aTGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTTAGCTTGCCCGAATCCTCGCACCACCGAGCCGCACCCCCAAGGTAAGCCTCGGGCAACAGCAAGCAGGGCATGTCGAGAAACAcc                                                   >  6:19927/1‑137 (MQ=255)                                                                             aTGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTTAGCTTGCCCGAATCCTAGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACAcc                                                   >  6:274494/1‑137 (MQ=255)                                                                             aTGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACAcc                                                   <  1:117039/137‑1 (MQ=255)                                                                             aTGACGCAAACCTGTCGACAAACGCCTGCAAGAACGCTCGCGTGTTGTCATTTATCTTGCCAGAAACCTAGAACCATCTCCCCCCAACGACCAGGTAAGCAGAGCGCATCTGCAAGCACGCCCTGTCCAGAAacaaa                                                   >  4:228153/1‑135 (MQ=255)                                                                                aCGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTTAGCTTTCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGc                                                >  4:164665/1‑137 (MQ=255)                                                                                  gCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCCAGGTAAGCCACGGGCATCTGCATGCAGGGCATGTCGAGCAACACCAGCgc                                              >  2:128483/1‑136 (MQ=255)                                                                                     aaCCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGCGACTg                                           <  2:78538/137‑1 (MQ=255)                                                                                     aaCCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGCGACTg                                           <  5:415990/137‑1 (MQ=255)                                                                                          gTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGCGACTGGCGCa                                      <  1:219011/137‑1 (MQ=255)                                                                                            cGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGCGACTGGCGCAcc                                    <  6:330401/137‑1 (MQ=255)                                                                                            cGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGCGACTGGCGCAcc                                    <  7:368524/137‑1 (MQ=255)                                                                                              aCAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTTAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGCTCCTGGCCCCCCGc                                  >  7:435036/1‑137 (MQ=255)                                                                                                                    gcgTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGCGACTGGCGCACCGCATGGTTGGCGCCAAAGccacca            >  7:136340/1‑137 (MQ=255)                                                                                                                     cgtggtGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCCCCGCCAAGGTAAGCCTCGGGCATATGCATGCAGGGCATGTCGAGAAACACCCCCGACTGGCGCACCGCATGGTTTGCGCCAAAGCCACCaa           >  2:440589/6‑137 (MQ=255)                                                                                                                         ttGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGCGACTGGCGCACCGCATGGTTGGCGCCAAAGCCACCAATCGc       <  3:164665/137‑1 (MQ=255)                                                                                                                         ttGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGCGACTGGCGCACCGCATGGTTGGCGCCAAAGCCACCAATCGc       <  7:207630/137‑1 (MQ=255)                                                                                                                         ttGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGCGACTGGCGCACCGCATGGTTGGCGCCAAAGCCACCAATCGc       <  3:354164/137‑1 (MQ=255)                                                                                                                              aTTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGCGACTGGCGCACCGCATGGTTGGCGCCAAAGCCACCAATCGCCCCCg  <  3:317820/137‑1 (MQ=255)                                                                                                                              aTTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGCGACTGGCGCACCGCATGGTTGGCGCCAAAGCCACCAATCGCCCCCg  <  3:350678/137‑1 (MQ=255)                                                                                                                                 |                                                                                                                                      GCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGCGACTGGCGCACCGCATGGTTGGCGCCAAAGCCACCAATCGCCCCCG  >  ACB122/4666971‑4667232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑