Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | ACB122 | 5,384,407 | 0 | C | G | 29.5% | 77.6 / 12.9 | 44 | intergenic (+528/‑330) | CJ019_05064/CJ019_05067 | putative protein/hypothetical protein |
Reads supporting (aligned to +/- strand): ref base C (5/26); new base G (13/0); total (18/26) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.65e-07 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Rejected as consensus: Frequency below/above cutoff threshold. |
GCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTG > ACB122/5384275‑5384542 | gCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGAGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGtttt > 2:25533/1‑137 (MQ=255) gcGGGCGGCTGCGTATTAAATCAGTAGCCACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTtgt < 2:117974/136‑1 (MQ=255) cggcTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTTGGCTg > 6:98649/1‑137 (MQ=255) aGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTc < 8:321828/137‑1 (MQ=255) acacGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTGTGGCTGGAATTTGGGCGCGGCGGGTCgg > 7:444739/1‑136 (MQ=255) acacGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGGTTTTTGTGGCTGGAATTTTGGGGCGGCGGGGCgc > 5:274828/1‑136 (MQ=255) aaGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTTTGCTGGTATTTTGGCGCTGCGGGGCGAAAAATAgg > 3:94891/1‑137 (MQ=255) aaGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTGTTTTTGTTCCTGGAATTTGGGCGCGGCGGGTGGaaaaaaagg > 8:18174/1‑133 (MQ=255) aGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGa < 8:233310/137‑1 (MQ=255) cacagcGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAACAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAAcc < 3:389506/132‑1 (MQ=255) agtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAAcc < 4:343158/137‑1 (MQ=255) gtaCCGACATAGGAGTACTGGCGGACGGGTGGTCAAATCCCCCCGGCTCCACCAAATCAAAAGAATAAGACGCCCTACCGCGTCTTTTTTTTATCCAGGAATTTTTGCCCTGCGGGTCGCAAAAAAGGAATTAAacc > 6:312135/1‑137 (MQ=255) aCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCt < 7:244237/137‑1 (MQ=255) cAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCGTGTTTTTTGGCTGGAATTTTGGCGCGGCGGGGCGAAAAATAGGATTTTAACCCattatt > 5:117442/1‑137 (MQ=255) ggAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAg < 6:70077/137‑1 (MQ=255) aGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTTGGCGGGAATTTTGGGGGTGCGGGTCGGAAAATCGGTTTTTACCCCGTTGTTGTTggg > 5:99433/1‑137 (MQ=255) aGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAggg < 2:117217/137‑1 (MQ=255) aGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAggg < 6:397956/137‑1 (MQ=255) tACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCg < 4:398495/137‑1 (MQ=255) tGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGttt < 3:226330/137‑1 (MQ=255) ggCGGACGGGGTTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTc < 5:285627/137‑1 (MQ=255) ggCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTc < 3:273347/137‑1 (MQ=255) gggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTTGGGTGGTATTTTGGGGCTGCGGGGGGAAAAATAGGATTTTAACCCTTTGTTGTTTGGGGGTTGCCAAAGATa > 2:63494/1‑137 (MQ=255) gggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTGTTGCGGGGATTTGGGGGGGGGGGGGGGGAAAATCGGGTTTTAACCCGTTGTTGTTGGGCGGTTGCCAAAGATa > 1:249616/1‑137 (MQ=255) gggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTGTGGCTGGAATTTGGGGGGGGCGGGTCGAAAAAACGGATTTAACCCCTTTCTTGTTTGGCGTTTGCCCAAGATa > 6:325268/1‑137 (MQ=255) gggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGGCGGGAATTTGGGGGGGGCGGGGCGAAAAAACTGATTTATACCCCTTCATTTAGGGCGTTTGCCAAAAATa > 6:219368/1‑137 (MQ=255) gTTCAAAACCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATAct < 2:53333/137‑1 (MQ=255) aaaTCCCCCCGGCTCCACCAAATCCAAAGACGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATActgcct < 1:186452/137‑1 (MQ=255) ccGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCGAGGAATTTTGGCGCAGGGGGGCGAAAAATAGGATTTAAAGCCTTTATTGTTGGGGGTTTCCCAAAGCAAATGCCAGCGccggg > 4:215887/1‑134 (MQ=255) caAATCCAAAGAAGAAGACGCCCTCGGGCGTGTTTTTTTTTTGGGGGTATTTTGGGGCGGCGGGTGGCAAAATCGGATTTTAAACCATTCTTTTATGGGGGTTGGCAAATATAATGCCTGCACCAGTTTTGCggttg > 4:32107/1‑133 (MQ=255) ccAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTTTCGTGGAATTTTGGGGGGGCGGGGCGAAAAATAGGCGTTAACCCCTTTGTTGGAGGGCGGTTGCCAAAGCTACTTCCTGCGCCGGTTTTGCGTGGGGGtgg > 6:384803/1‑135 (MQ=255) gaagaagaCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt < 4:94891/137‑1 (MQ=255) gaagaagaCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt < 3:385265/137‑1 (MQ=255) gaagaagaCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt < 2:417178/137‑1 (MQ=255) gaagaagaCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt < 8:373319/137‑1 (MQ=255) caagaagaCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCTCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt < 2:176814/136‑1 (MQ=255) caagaagaCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt < 7:77378/136‑1 (MQ=255) aagaagaCGCCCTCGGGCGTGGTTTTTTGTTGCGGGTATTTGGGGGCGGCGGGGGGGAAAATAGGCTTTAACCCCGTTCTTGTTGGGGGGTTGCCAAAGATACTTCCGGGGGCGTTTTTTCGTTGCGGGGAtttttt > 8:278681/1‑137 (MQ=255) aagaagaCGCCCTCGGGCGTGGTTTTTTGTGGCGGGTATTTTGGCGCGGCGGGGGGAAAAAAAGGATTTTACCCCCGTGGTGTATGGCGTTTACCAAAGATAATGCCTGCGGCTGGTTTACGGTGCGGTCAtttttt > 8:83566/1‑137 (MQ=255) gaCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAggg < 3:76499/137‑1 (MQ=255) cccTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGtttt < 2:16749/137‑1 (MQ=255) cTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAg < 1:226319/137‑1 (MQ=255) gggCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGtct < 8:193198/137‑1 (MQ=255) tCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTg < 4:193267/137‑1 (MQ=255) | GCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTG > ACB122/5384275‑5384542 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |