Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,384,4070CG29.5% 77.6 / 12.9 44intergenic (+528/‑330)CJ019_05064/CJ019_05067putative protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (5/26);  new base G (13/0);  total (18/26)
Fisher's exact test for biased strand distribution p-value = 1.65e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTG  >  ACB122/5384275‑5384542
                                                                                                                                    |                                                                                                                                       
gCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGAGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGtttt                                                                                                                                     >  2:25533/1‑137 (MQ=255)
     gcGGGCGGCTGCGTATTAAATCAGTAGCCACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTtgt                                                                                                                                <  2:117974/136‑1 (MQ=255)
          cggcTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTTGGCTg                                                                                                                           >  6:98649/1‑137 (MQ=255)
                              aGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTc                                                                                                       <  8:321828/137‑1 (MQ=255)
                                acacGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTGTGGCTGGAATTTGGGCGCGGCGGGTCgg                                                                                                     >  7:444739/1‑136 (MQ=255)
                                acacGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGGTTTTTGTGGCTGGAATTTTGGGGCGGCGGGGCgc                                                                                                     >  5:274828/1‑136 (MQ=255)
                                        aaGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTTTGCTGGTATTTTGGCGCTGCGGGGCGAAAAATAgg                                                                                             >  3:94891/1‑137 (MQ=255)
                                        aaGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTGTTTTTGTTCCTGGAATTTGGGCGCGGCGGGTGGaaaaaaagg                                                                                             >  8:18174/1‑133 (MQ=255)
                                         aGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGa                                                                                            <  8:233310/137‑1 (MQ=255)
                                                cacagcGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAACAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAAcc                                                                                     <  3:389506/132‑1 (MQ=255)
                                                agtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAAcc                                                                                     <  4:343158/137‑1 (MQ=255)
                                                 gtaCCGACATAGGAGTACTGGCGGACGGGTGGTCAAATCCCCCCGGCTCCACCAAATCAAAAGAATAAGACGCCCTACCGCGTCTTTTTTTTATCCAGGAATTTTTGCCCTGCGGGTCGCAAAAAAGGAATTAAacc                                                                                    >  6:312135/1‑137 (MQ=255)
                                                   aCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCt                                                                                  <  7:244237/137‑1 (MQ=255)
                                                        cAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCGTGTTTTTTGGCTGGAATTTTGGCGCGGCGGGGCGAAAAATAGGATTTTAACCCattatt                                                                             >  5:117442/1‑137 (MQ=255)
                                                            ggAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAg                                                                         <  6:70077/137‑1 (MQ=255)
                                                              aGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTTGGCGGGAATTTTGGGGGTGCGGGTCGGAAAATCGGTTTTTACCCCGTTGTTGTTggg                                                                       >  5:99433/1‑137 (MQ=255)
                                                              aGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAggg                                                                       <  2:117217/137‑1 (MQ=255)
                                                              aGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAggg                                                                       <  6:397956/137‑1 (MQ=255)
                                                                tACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCg                                                                     <  4:398495/137‑1 (MQ=255)
                                                                   tGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGttt                                                                  <  3:226330/137‑1 (MQ=255)
                                                                    ggCGGACGGGGTTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTc                                                                 <  5:285627/137‑1 (MQ=255)
                                                                    ggCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTc                                                                 <  3:273347/137‑1 (MQ=255)
                                                                             gggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTTGGGTGGTATTTTGGGGCTGCGGGGGGAAAAATAGGATTTTAACCCTTTGTTGTTTGGGGGTTGCCAAAGATa                                                        >  2:63494/1‑137 (MQ=255)
                                                                             gggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTGTTGCGGGGATTTGGGGGGGGGGGGGGGGAAAATCGGGTTTTAACCCGTTGTTGTTGGGCGGTTGCCAAAGATa                                                        >  1:249616/1‑137 (MQ=255)
                                                                             gggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTGTGGCTGGAATTTGGGGGGGGCGGGTCGAAAAAACGGATTTAACCCCTTTCTTGTTTGGCGTTTGCCCAAGATa                                                        >  6:325268/1‑137 (MQ=255)
                                                                             gggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGGCGGGAATTTGGGGGGGGCGGGGCGAAAAAACTGATTTATACCCCTTCATTTAGGGCGTTTGCCAAAAATa                                                        >  6:219368/1‑137 (MQ=255)
                                                                               gTTCAAAACCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATAct                                                      <  2:53333/137‑1 (MQ=255)
                                                                                   aaaTCCCCCCGGCTCCACCAAATCCAAAGACGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATActgcct                                                  <  1:186452/137‑1 (MQ=255)
                                                                                           ccGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCGAGGAATTTTGGCGCAGGGGGGCGAAAAATAGGATTTAAAGCCTTTATTGTTGGGGGTTTCCCAAAGCAAATGCCAGCGccggg                                          >  4:215887/1‑134 (MQ=255)
                                                                                                     caAATCCAAAGAAGAAGACGCCCTCGGGCGTGTTTTTTTTTTGGGGGTATTTTGGGGCGGCGGGTGGCAAAATCGGATTTTAAACCATTCTTTTATGGGGGTTGGCAAATATAATGCCTGCACCAGTTTTGCggttg                                >  4:32107/1‑133 (MQ=255)
                                                                                                          ccAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTTTCGTGGAATTTTGGGGGGGCGGGGCGAAAAATAGGCGTTAACCCCTTTGTTGGAGGGCGGTTGCCAAAGCTACTTCCTGCGCCGGTTTTGCGTGGGGGtgg                           >  6:384803/1‑135 (MQ=255)
                                                                                                               gaagaagaCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt                      <  4:94891/137‑1 (MQ=255)
                                                                                                               gaagaagaCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt                      <  3:385265/137‑1 (MQ=255)
                                                                                                               gaagaagaCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt                      <  2:417178/137‑1 (MQ=255)
                                                                                                               gaagaagaCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt                      <  8:373319/137‑1 (MQ=255)
                                                                                                               caagaagaCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCTCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt                      <  2:176814/136‑1 (MQ=255)
                                                                                                               caagaagaCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt                      <  7:77378/136‑1 (MQ=255)
                                                                                                                aagaagaCGCCCTCGGGCGTGGTTTTTTGTTGCGGGTATTTGGGGGCGGCGGGGGGGAAAATAGGCTTTAACCCCGTTCTTGTTGGGGGGTTGCCAAAGATACTTCCGGGGGCGTTTTTTCGTTGCGGGGAtttttt                     >  8:278681/1‑137 (MQ=255)
                                                                                                                aagaagaCGCCCTCGGGCGTGGTTTTTTGTGGCGGGTATTTTGGCGCGGCGGGGGGAAAAAAAGGATTTTACCCCCGTGGTGTATGGCGTTTACCAAAGATAATGCCTGCGGCTGGTTTACGGTGCGGTCAtttttt                     >  8:83566/1‑137 (MQ=255)
                                                                                                                     gaCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAggg                <  3:76499/137‑1 (MQ=255)
                                                                                                                         cccTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGtttt            <  2:16749/137‑1 (MQ=255)
                                                                                                                           cTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAg          <  1:226319/137‑1 (MQ=255)
                                                                                                                              gggCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGtct       <  8:193198/137‑1 (MQ=255)
                                                                                                                                   tCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTg  <  4:193267/137‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                       
GCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTG  >  ACB122/5384275‑5384542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: