| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 5,989,859 | 0 | C | G | 54.5% | 3.1 / 12.0 | 11 | intergenic (‑313/+28) | nfdA/CJ019_05610 | N‑substituted formamide deformylase/Arylesterase |
| Reads supporting (aligned to +/- strand): ref base C (5/0); new base G (0/6); total (5/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.16e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTGCGGCGCCCCGGCATACACCTTCAGCTCGGCGCCACGAAT > ACB122/5989786‑5989991 | ccccTGGGCAAGTTTTTTGCTCCCTGGGCGGGCGGCTGCGCGGGAAAACCCGCTCCTACAACGTGTGTTGGCGGGGGTTAACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAg < 2:278554/136‑1 (MQ=255) cgtGGTCACGCTTTTGGCGGCCGGGGCCGGCCTGTGCGCGGTAAAACCCGCTCAAACAACGTATGTAGGAGGGGTTTAACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAgct < 8:122244/135‑1 (MQ=255) ccacgCTTTTCGCCGCCGGGGCCGGACGGTGCGCGGGAACCCCCCCACCAACACCGTCTGATGGAGGGTGTTAACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCtgctgc < 7:183426/133‑1 (MQ=255) ttttttGCGCCCGGGCCCGGCCCCTGCGCGGTAACCCCCCCTCCAACACCTTCTGTCGCTGGGGTTTAACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATg < 5:247790/136‑1 (MQ=255) gggcgtGGGCGGGCCGTTGCGCGGCTAAACCCGACCAAACATCGTGTTTCGGCGGGGTTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACt < 7:150434/132‑1 (MQ=255) accGTGTGTCGGTGGGGTTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTGCGGCGCCCCGGCATACACCTTCAGCTCgg < 3:100618/135‑1 (MQ=255) gTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTGCGGCGCCCCGGCATACACCTTCAGCTCGGCGCCACg > 2:171886/1‑137 (MQ=255) aGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTGCGGCGCCCCGGCATACACCTTCAGCTCGGCGCCACGaa > 7:13646/1‑137 (MQ=255) aGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTGCGGCGCCCCGGCATACACCTTCAGCTCGGCGCCACGaa > 8:222924/1‑137 (MQ=255) aGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACAGTGCGGCGCCCCGGCATACACCTTCAGCTCGGCGCCACGaa > 2:219208/1‑137 (MQ=255) ggAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTGCGGCGCCCCGGCATACACCTTCAGCTCGGCGCCACGAAt > 2:49547/1‑137 (MQ=255) | GCCCTGGTCAAGCTTTTTGCTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAACGTCTGTAGGAGCGGGTTTACCCGCGAATGTTTCCCACAATTACCGCTGAAGGAACGCCAGCAAGTCCTCATTCAGCTGCTGCGCATGAGTCACTGCAAAACCGTGCGGCGCCCCGGCATACACCTTCAGCTCGGCGCCACGAAT > ACB122/5989786‑5989991 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |