Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 120,975 Δ1 bp 100% intergenic (+272/+100) polA → / ← engB DNA polymerase I/putative GTP‑binding protein EngB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB122120,9750T.100.0% 169.1 / NA 37intergenic (+272/+100)polA/engBDNA polymerase I/putative GTP‑binding protein EngB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (25/12);  total (25/12)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AATGGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGTTCCATC  >  ACB122/120854‑121105
                                                                                                                         |                                                                                                                                  
aaTGGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCgg                                                                                                                      >  2:49141/1‑135 (MQ=255)
   ggCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCCTACGAAGTCCGGGG‑TTTGTTTTGCCCGGAAtt                                                                                                                  >  7:18295/1‑136 (MQ=255)
                      tGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTtct                                                                                               >  5:142123/1‑136 (MQ=255)
                        cTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTcg                                                                                             <  1:70142/136‑1 (MQ=255)
                              tgttgtGTTAAGTGTTTGCAGATATCTGGACCCCGCCGTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt                                                                                        <  5:102936/135‑1 (MQ=255)
                              tgttgtGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt                                                                                        <  5:44101/135‑1 (MQ=255)
                                ttgtGTTAAGTGTTGGCAGATAGCTGGACCCCGCCCTAGCGGGCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGc                                                                                      <  7:75527/135‑1 (MQ=255)
                                      tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCCTACGAAGTCCAGCG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGCAAGCGATGTc                                                                                >  2:61599/1‑135 (MQ=255)
                                      tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCCAACGAAGTCCGGCG‑GTTTTTTTGCCCGGAATTTTCCGGGAGCGGGTTTTCTCGCGAATGCGATGtat                                                                               >  8:152129/1‑134 (MQ=255)
                                      tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTTACGGGAGCGGGTTTTCTCGCGAATGCGATGTc                                                                                >  5:196275/1‑135 (MQ=255)
                                      tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGTGGGTTTTCTCGCGAATGCGATGTc                                                                                >  6:10842/1‑135 (MQ=255)
                                      tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATg                                                                                  >  2:157006/1‑133 (MQ=255)
                                      tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTc                                                                                >  2:55986/1‑135 (MQ=255)
                                      tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTc                                                                                >  6:32179/1‑135 (MQ=255)
                                      tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTc                                                                                >  4:102051/1‑135 (MQ=255)
                                      tAAGTGTTGGCAGATATCTGGACACCGCCCTAGCGGGCCGGACATGGACCACGCACTTCCCCCTCCCCATACGCAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTATCCAGAATGCGATGTc                                                                                >  7:184672/1‑135 (MQ=255)
                                        aGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGaa                                                                             >  1:52423/1‑136 (MQ=255)
                                        aGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGaa                                                                             >  3:163463/1‑136 (MQ=255)
                                                cAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTtct                                                                                               <  1:73633/110‑1 (MQ=255)
                                                cAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACAccgc                                                                     >  5:178855/1‑136 (MQ=255)
                                                cAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTATCTCGCCAATGCGATGTCGAAAACAccgc                                                                     >  5:63510/1‑136 (MQ=255)
                                                cAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCAGGGAGCGGGTTTtct                                                                                               >  2:73633/1‑110 (MQ=255)
                                                  gATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGCGCGGGTTTTCTCGCGAATGGGATGTCGAATACAccgccc                                                                   >  6:123766/1‑135 (MQ=255)
                                                      tCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCAt                                                                <  2:52423/135‑1 (MQ=255)
                                                          gcccccGCCCTAGCCGGCCGTACTTGGACCCCGAACTTCCCCCACCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCGCGCGATTGCGATGTCTAATACACCGCCGCATTcgcg                                                           <  1:110004/134‑1 (MQ=255)
                                                                  ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCCTTCGCGGGGAAAcc                                                   >  5:164831/1‑136 (MQ=255)
                                                                  ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAccc                                                   >  8:85670/1‑136 (MQ=255)
                                                                  ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAAcc                                                   >  2:114242/1‑136 (MQ=255)
                                                                   cTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAcccc                                                  >  7:50133/1‑136 (MQ=255)
                                                                   cTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGGTTTCTCGCGGATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAAccc                                                  >  5:149103/1‑136 (MQ=255)
                                                                      gCGGTCCGTACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCTAATACACCGCCGCATTCGCGGGTAAACCCg                                                 <  3:144906/134‑1 (MQ=255)
                                                                      gCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCgg                                                <  6:142123/135‑1 (MQ=255)
                                                                       cGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCAt                                                                <  3:82178/118‑1 (MQ=255)
                                                                       cGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCAt                                                                >  4:82178/1‑118 (MQ=255)
                                                                        ggTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACTAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGt                                              >  4:93720/1‑135 (MQ=255)
                                                                            cGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGTGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGgc                                         <  5:10842/136‑1 (MQ=255)
                                                                                       cccGAACTTCCCCCTCCCCATACGAAGTCCGGGGttttttttg                                                                                                                            <  7:174956/43‑2 (MQ=255)
                                                                                       cccGAACTTCCCCCTCCCCATACGAAGTCCGGGGttttttttg                                                                                                                            >  8:174956/1‑42 (MQ=255)
                                                                                                      ccccATACGAAGTCCGGGG‑TTTTTTTTGCCCGTAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATCCACCGCCGCATTCGCGGGTAAACCCGGGGCAGGCGCAAACCACTAAACGACCGGCTTGTc               <  8:8006/136‑1 (MQ=39)
                                                                                                                    ccggggTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGTtccatc  >  7:36360/3‑136 (MQ=255)
                                                                                                                     cggggTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCCAACCACTAAACGACCGGCTTGTCTTCCAGTtccatc  >  3:177400/2‑135 (MQ=255)
                                                                                                                         |                                                                                                                                  
AATGGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGTTCCATC  >  ACB122/120854‑121105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑