Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 5,622,704 Δ1 bp 7.4% coding (212/1629 nt) putP ← Sodium/proline symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,622,7040A. 7.4% 177.2 / 7.4 54coding (212/1629 nt)putPSodium/proline symporter
Reads supporting (aligned to +/- strand):  ref base A (22/28);  new base . (2/2);  total (24/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCACCGGGCAGGCCCATCAGCAGCCAGCCACTCATGTCGGAGGCACCGGCAGACAGCGCGGTAACCACGCTACCCAGGCTGCGACCACCCAGAATGTAATCGGAAAGGTTGTTGGTAGCGCGATAGGCGGCGAAGCCGATCAGCACCATTGCTGCGATGTAGATCACGAAGGTGATCGTTAGTGGATTGCCCATGCGTGTGCCCTGGCGTTTGTTTTTATCATGCGCAACCACACCGTTGGTGGTTGCACCCAGGCGGCGAATG  >  ACB122/5622571‑5622835
                                                                                                                                     |                                                                                                                                   
ggCACCGGGCAGGCCCATCAGCAGCCAGCCACTCATGTCGGAGGCACCGGCAGACAGCGCGGTAACCACGCTACCCAGGCTGCGACCACCCAGAATGTAATCGGAAAGGTTGTTGGTAGCGCGATAGGCGGCGAAg                                                                                                                                   <  3:52740/136‑1 (MQ=255)
      gggCAGGCCCATCAGCAGCCAGCCACTCATGTCGGAGGCACCGGCAGACAGCGCGGTAACCACGCTACCCAGGCTGCGACCACCCAGAATGTAATCGGAAAGGTTGTTGGTAGCGCGATAGGCGGCGAAGCCGAt                                                                                                                              >  1:173021/1‑135 (MQ=255)
        gCAGGCCCATCAGCAGCCAGCCACTCATGTCGGAGGCACCGGCAGACAGCGCGGTAACCACGCTACCCAGGCTGCGACCACCCAGAATGTAATCGGAAAGGTTGTTGGTAGCGCGATAGGCGGCGAAGCCGATCAg                                                                                                                           >  4:194012/1‑136 (MQ=255)
        gCAGGCCCATCAGCAGCCAGCCACTCATGTCGGAGGCACCGGCAGACAGCGCGGTAACCACGCTACCCAGGCTGCGACCACCCAGAATGTAATCGGAAAGGTTGTTGGTAGCGCGATAGGCGGCGAAGCCGATCAg                                                                                                                           <  2:44498/136‑1 (MQ=255)
             cccATCAGCAGCCAGCCACTCATGTCGGAGGCACCGGCAGACAGCGCGGTAACCACGCTACCCAGGCTGCGACCACCCAGAATGTAATCGGAAAGGTTGTTGGTAGCGCGATAGGCGGCGAAGCCGATCAGCACCa                                                                                                                      <  4:107362/136‑1 (MQ=255)
               cATCAGCAGCCAGCCACTCATGTCGGAGGCACCGGCAGACAGCGCGGTAACCACGCTACCCAGGCTGCGACCACCCAGAATGTAATCGGAAAGGTTGTTGGTAGCGCGATAGGCGGCGAAGCCGATCAGCACCAtt                                                                                                                    >  5:140879/1‑136 (MQ=255)
                     cagccagcCACTCATGTCGGAGGCACCGGCAGACAGCGCGGTAACCACGCTACCCAGGCTGCGACCACCCAGAATGTAATCGGAAAGGTTGTTGGTAGCGCGATAGGCGGCGAAGCCGATCAGCACCATtgctgc                                                                                                               >  4:25122/1‑135 (MQ=255)
                           gccaCTCATGTCGGAGGCACCGGCAGACAGCGCTGTAACCACGCTACCCAGGCTGCGACCACCCAGAATGTAATCGGAAAGGTTGTTGGTAGCGCGATAGGCGGCGAAGCCGATCAGCACCa                                                                                                                      <  6:194959/122‑1 (MQ=255)
                           gccaCTCATGTCGGAGGCACCGGCAGACAGCGCGGTAACGACGCTACCCAGGCTGCGACCACCCAGAATGTAATCGGAAAGGTTGTTGGTAGCGCTATAGGCGGCGAAGCCGATCAGCACCATTGCTGCGAt                                                                                                            <  1:24735/132‑1 (MQ=255)
                           gccaCTCATGTCGGAGGCACCGGCAGACAGCGCGGTAACCACGCTACCCAGGCTGCGACCACCCAGAATGTAATCGGAAAGGTTGTTGGTAGCGCGATAGGCGGCGAAGCCGATCAGCACCa                                                                                                                      >  5:194959/1‑122 (MQ=255)
                           gccaCTCATGTCGGAGGCACCGGCAGACAGCGCGGTAACCACGCTACCCAGGCTGCGACCACCCAGAATGTAATCGGAAAGGTTGTTGGTAGCGCGATAGGCGGCGAAGCCGATCAGCACCATTGCTGCGAt                                                                                                            >  2:24735/1‑132 (MQ=255)
                           gccaCTCATGTCGGAGGCACCGGCAGACAGCGCGGTAACCACGCTACCCAGGCTGCGACCACCCAGAATGTAATCGGAAAGGTTGTTGGTAGCGCGATAGGCGGCGAAGCCGATCAGCACCATTGCTGCGATg                                                                                                           >  5:199940/1‑133 (MQ=255)
                             caCTCATGTCGGAGGCACCGGCAGACAGCGCGGTAACCACGCTACCCAGGCTGCGACCACCCAGAATGTAATCGGAAAGGTTGTTGGTAGCGCGATAGGCGGCGAAGCCGATCAGCACCATTGCTGCGATg                                                                                                           >  1:131659/1‑131 (MQ=255)
                                      cGGAGGCACCGGCAGACAGCGCGGTAACCACGCTACCCAGGCTGCGACCACCCAGAATGTAATCGGAAAGGTTGTTGGTAGCGCGATAGGCGGCGAAGCCGATCAGCACCATTGCTGCGATGTAGATCACGAAGGt                                                                                             <  2:173021/136‑1 (MQ=255)
                                       ggAGGCACCGGCAGACAGCGCGGTAACCACGCTACCCAGGCTGCGACCACCCAGAATGTAATCGGAAAGGTTGTTGGTAGCGCGATAGGCGGCGAAGCCGATCAGCACCATTGCTGCGATGTAGATCACGAAGGt                                                                                             <  4:93236/135‑1 (MQ=255)
                                       ggAGGCACCGGCAGACAGCGCGGTAACCACGCTACCCAGGCTGCGACCACCCAGAATGTAATCGGAAAGGTTGTTGGTAGCGCGATAGGCGGCGAAGCCGATCAGCACCATTGCTGCGATGTAGATCACGAAGGt                                                                                             >  1:89986/1‑135 (MQ=255)
                                         agccACCCGCAGACACCGCGGTAACCACGCTACCCAGGCTGCGACCACCCAGAATGTAATCGGAAAGGTAGATGGTAGCGCGATAGGCGGCGAAGCCGATCAGCACCATAGCTGCGATGTAGATCACGAACGTGAt                                                                                          >  8:140035/4‑136 (MQ=255)
                                             aCCGGCAGACAGCGCGGTAACCACGCTACCCAGGCTGCGACCACCCAGAATGTAATCGGAAAGGTTGTTGGTAGCGCGATAGGCGGCGAAGCCGATCAGCACCATTGCTGCGATGTAGATCACGAAGGTGATCGt                                                                                       >  1:97500/1‑135 (MQ=255)
                                                ggCAGACAGCGCGGTAACCACGCTACCCAGGCTGCGACCACCCAGAATGTAATCGGAAAGGTTGTTGGTAGCGCGATAGGCGGCGAAGCCGATCAGCACCATTGCTGCGATGTAGATCACGAAGGTGATCGTTAGt                                                                                   <  2:97500/136‑1 (MQ=255)
                                                ggCAGACAGCGCGGTAACCACGCTACCCAGGCTGCGACCACCCAGAATGTAATCGGAAAGGTTGTAGGTAGCGCGATAGGCGGCGAAGCCGATCAGCACCATTGCTGCGATGTAGATCACGAAGGTGATCGTTAGt                                                                                   <  8:175994/136‑1 (MQ=255)
                                                ggCAGACAGCGCGGGAACCACGCTACCCAGGCTGCGACCACCCAGAATGTAATCGGAAATGTTGTTGGTAGCGCGATAGGCGGCGAAGCCGATCATCACCATTGCTGCGATGTAGATCACGAAGGTGATCGTTAGt                                                                                   <  4:115299/136‑1 (MQ=255)
                                                     aCAGCGCGGTAACCACGCTACCCAGGCTGCGACCACCCAGAATGTAATCGGAAAGGTTGTTGGTAGCGCGATAGGCGGCGAAGCCGATCAGCACCATTGCTGCGATGTAGATCACGAAGGTGATCGTTAGTGGAtt                                                                              <  6:199940/136‑1 (MQ=255)
                                                      cAGCGCGGTAACCACGCTACCCAGGCTGCGACCACCCAGAATGTAATCGGAAAGGTTGTTGGTAGCGCGATAGGCGGCGAAGCCGATCAGCACCATTGCTGCGATGTAGATCACGAAGGTGATCGTTAGTGGATTg                                                                             <  5:170939/136‑1 (MQ=255)
                                                          gcgGTAACCACGCTACCCAGGCTGCGACCACCCAGAATGTAATCGGAAAGGTTGTTGGTAGCGCGATAGGCGGCGAAGCCGATCAGCACCATTGCTGCGATGTAGATCACGAAGGTGATCGTTAGTGGATTGCCCa                                                                         <  4:65703/136‑1 (MQ=255)
                                                            ggTAACCACGCTACCCAGGCTGCGACCACCCAGAATGTAATCGGAAAGGTTGTTGGTAGCGCGATAGGCGGCGAAGCCGATCAGCACCATTGCTGCGATGTAGATCACGAAGGTGATCGTTAGTGGATTGCCCATg                                                                       <  8:89234/136‑1 (MQ=255)
                                                            ggTAACCACGCTACCCAGGCTGCGACCACCCAGAATGTAATCGGAAAGGTTGTTGGTAGCGCGATAGGCGGCGAAGCCGATCAGCACCATTGCTGCGATGTAGATCACGAAGGTGATCGTTAGTGGATTGCCCATg                                                                       >  8:77140/1‑136 (MQ=255)
                                                                 ccACGCTACCCAGGCTGCGACCACCCAGAATGTAATCGGAAAGGTTGTTGGTAGCGCGATAGGCGGCGAAGCCGATCAGCACCATTGCTGCGATGTAGATCACGAAGGTGATCGTTAGTGGATTGCCCATGCgtgt                                                                  <  7:194530/136‑1 (MQ=255)
                                                                    cGCTACCCAGGCTGCGACCACCCAGAATGTAATCGGAAAGGTTGTTGGTAGCGCGATAGGCGGCGAAGCCGATCAGCACCATTGCTGCGATGTAGATCACGAAGGTGATCGTTAGTGGATTGCCCATGCGTGTGcc                                                               >  7:98554/1‑136 (MQ=255)
                                                                             ggCTGCGACCACCCAGAATGTAATCGGAAAGGTTGTTGGTAGCGCGATAGGCGGCGAAGCCGATCAGCACCATTGCTGCGATGTAGATCACGAAGGTGATCGTTAGTGGATTGCCCATGCGTGTGCCCTGGCgttt                                                      >  8:32958/1‑136 (MQ=255)
                                                                                    accaccCAGAATGTATTCGGAAAGGTTGTTGGTAGCTCGATAGGCGGCGAAGACGATCAGCACCATTGCTGCGATGTAGATCACGAAGGAGATCGTTAGTGGATTGCCCATGCGTGTGCCCTGTCGTTTTTGTTTa                                               >  2:160592/1‑136 (MQ=255)
                                                                                      caccCAGAATGTAATCGGAAAGGTTGTTGGTAGCGCGATAGGCGGCGAAGCCGATCAGCACCATTGCTGCGATGTAGATCACGAAGGTGATCGTTAGTGGATTGCCCATGCGTGTGCCCTGGCGTTTGTTTTTATc                                             <  3:194012/136‑1 (MQ=255)
                                                                                      caccCAGAATGTAATCGGAAAGGTTGTTGGTAGCGCGATAGGCGGCGAAGCCGATCAGCACCATTGCTGCGATGTAGATCACGAAGGTGATCGTTAGTGGATTGCCCATGCGTGTGCCCTGGCGTTTGTTTTTATc                                             <  6:140879/136‑1 (MQ=255)
                                                                                      caccCAGAATGTAATCGGAAAGGTTGTTGGTAGCGAGATAGGCGGCG‑AGCCGATCAGCACCATTGCTGCGATGTAGCTCACGAAGGTGATCGTTAGTGGATTGCCCATGCGTGTGCCCTGGCGGGGGtttttttga                                            >  5:14707/1‑132 (MQ=255)
                                                                                      caccCAGAATGTAATCGGAAAGGTTGTTGGTAGCGAGATAGGCGGCG‑AGCCGATCAGCACCATTGCTGCGATGTAGATCACGAAGGTGATCGTTAGTGGATTGCCCATGCGTGTGCCCTGGCGTGTGGTTTTATCa                                            >  5:14708/1‑136 (MQ=255)
                                                                                       accCAGAATGTAATCGGAAAGGGTGTTGGAATCGCGATAGGCGGCGAAGCCGATCAGCACCATTGCTGCGATGTAGATCACGAAGGTGATCGTTAGGGGATTGCCCATGCGTGAGCCCTGGCGTTTGTTTTTATCa                                            >  7:22352/1‑136 (MQ=255)
                                                                                        cccAGAATGTAATCGGAAAGGTTGTTGGTAGCGCGATAGGCGGCGAAGCCGATCAGCACCATTGCTGCGATGTAGATCACGAAGGTGATCGTTAGTGGATTGCCCATGCGTGTGCCCTGGCGTTTGTTTTTATCAt                                           <  7:88589/136‑1 (MQ=255)
                                                                                           aGAATGTAATCGGAAAGGTTGTTGGTAGCGCGATAGGCGGCGAAGCCGATCAGCACCATTGCTGCGATGTAGATCTCGAAGGTGATCGTTAGTGGATTGCCCATGCGTGTGCCCTGGCGTTTGTTTTTATCATgcg                                        >  5:88457/1‑136 (MQ=255)
                                                                                              aTGTAATCGGAAAGGGTGTTGGTAGCGCGATTGGCGGCGAAGCCGATCAGCACCATTGCTGCGATGTAGATCACGAAGGTGATCGTTAGTGGATTGCCCATGCGTGTGCCCTGGCGTTTGTTTTTATCATGCGCaa                                     <  6:201237/136‑1 (MQ=255)
                                                                                               tGTAATCGGAAAGGTTGTTGGTAGCGCGATAGGCGGCGAAGCCGATCAGCACCAtt                                                                                                                    >  7:135575/1‑56 (MQ=255)
                                                                                               tGTAATCGGAAAGGTTGTTGGTAGCGCGATAGGCGGCGAAGCCGATCAGCACCAtt                                                                                                                    <  8:135575/56‑1 (MQ=255)
                                                                                                 tAATCGGAAAGGTTGTTGGTAGCGCGATAGGCGGCGAAGCCGATCAGCACCATTGCTGCGATGTAGATCACGAAGGTGATCGTTAGTGGATTGCCCATGCGTGTGCCCTGGCGTTTGTTTTTATCATGCGCAAcc                                   <  2:131659/135‑1 (MQ=255)
                                                                                                 tAATCGGAAAGGTTGTTGGTAGCGCGATAGGCGGCGAAGCCGATCAGCACCATTGCTGCGATGTAGATCACGAAGGTGATCGTTAGTGGATTGCCCATGCGTGTGCCCTGGCGTTTGTTTTTATCATGCGCAAcc                                   <  1:116476/135‑1 (MQ=255)
                                                                                                       gAAAGGTTGTTGGTAGCGCGATAGGCGGCGAAGCCGATCAGCACCATTGCTGCGATGTAGATCACGAAGGTGATCGTTAGTGGATTGCCCATGCGTGTGCCCTGGCGTTTGTTTTTATCATGCGCAACCACACCGt                            <  2:8510/136‑1 (MQ=255)
                                                                                                          aGGTTGTTGGTAGCGCGATAGGCGGCGAAGCCGATCAGCACCATTGCTGCGATGTAGATCACGAAGGTGATCGTTAGTGGATTGCCCATGCGTGTGCCCTGGCGTTTGTTTTTATCATGCGCAACCACACCGTtgg                         <  7:175995/136‑1 (MQ=255)
                                                                                                           tgttgttGGTAGCGCGATAGGCGGCGAAGCCGATCAGCACCATTGCTGAGATGTAGATCACGAATGTGATCGTTAGTTGATTGCCCATGCGTGTGACCTGTCGATTGTTTTTATCATGCGCAACCACACCGTtggt                        <  1:157040/135‑1 (MQ=255)
                                                                                                                 tgGTAGCGCGATAGGCGGCGAAGCCGATCAGCACCATtgct                                                                                                                 <  5:128662/41‑1 (MQ=255)
                                                                                                                 tgGTAGCGCGATAGGCGGCGAAGCCGATCAGCACCATtgct                                                                                                                 >  6:128662/1‑41 (MQ=255)
                                                                                                                        gcgATAGGCGGCGAAGCCGATCAGCACCATTGCTGCGATGTATATCACGAAGGTGATCGTTAGTGGATTGCCCATGCGTGTGCCCTGGCGTTTGTTTTTATCATGCGCAACCACACCGTTGGTGGTTGCCCCCAgg           >  8:202537/1‑136 (MQ=255)
                                                                                                                        gcgATAGGCGGCGAAGCCGATCAGCACCATTGCTGCGATGTAGATCACGAAGGTGATCGTTAGt                                                                                   <  7:203502/64‑1 (MQ=255)
                                                                                                                        gcgATAGGCGGCGAAGCCGATCAGCACCATTGCTGCGATGTAGATCACGAAGGTGATCGTTAGt                                                                                   >  8:203502/1‑64 (MQ=255)
                                                                                                                        gagATAGGCGGCG‑TGCCGATCAGCACCATTGGTGCGATGTAGATCACGAAGGTGATCGTTAGTGGATTGCCCATGCGGGTGCCCTGGCGTTTGTTTTTATCATGCTCAACCACTCCGTTGGTGGTTGCACCCAggc          <  6:14707/134‑1 (MQ=255)
                                                                                                                        gagATAGGCGGCG‑AGCCGATCAGCACCATTGCTGCGATGTAGATCACGAAGGTGATCGTTAGTGGATTGCCCATGCGTGTGCCCTGGCGTTTGTTTTTATCATGCGCAACCACACCGTTGGTGGTTGCACCCAggc          <  6:14708/134‑1 (MQ=255)
                                                                                                                              ggcggcGAAGCCGATCAGCACCATTGCAGCGATGTAGATCACGAAGGTGTTCGATAGTGGATTGCCCATGCGTGTGCCCTGGCGTTTGTTTTTATCATGCGCAACCACACCGTTGGTGGTTGCACCCAGGCGGCGa     <  7:140035/136‑1 (MQ=255)
                                                                                                                                 ggcgAAGCCGATCAGCACCATTGCTGCGATGTAGATCACGAAGGTGATCGTTAGTGGATTGCCCATGCGTGTGCCCTGGCGTTTGTTTTTTTCAGTCGCCACCCCCCCGGTGGTGGGTGGCCCCCGgcggcgcgag  >  6:109476/1‑132 (MQ=255)
                                                                                                                                     |                                                                                                                                   
GGCACCGGGCAGGCCCATCAGCAGCCAGCCACTCATGTCGGAGGCACCGGCAGACAGCGCGGTAACCACGCTACCCAGGCTGCGACCACCCAGAATGTAATCGGAAAGGTTGTTGGTAGCGCGATAGGCGGCGAAGCCGATCAGCACCATTGCTGCGATGTAGATCACGAAGGTGATCGTTAGTGGATTGCCCATGCGTGTGCCCTGGCGTTTGTTTTTATCATGCGCAACCACACCGTTGGTGGTTGCACCCAGGCGGCGAATG  >  ACB122/5622571‑5622835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑