| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 627,228 | T→G | 48.8% | S162A (TCG→GCG) | acoR_1 → | Acetoin catabolism regulatory protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 627,228 | 0 | T | G | 48.8% | ‑4.3 / 3.5 | 20 | S162A (TCG→GCG) | acoR_1 | Acetoin catabolism regulatory protein |
| Reads supporting (aligned to +/- strand): ref base T (5/5); new base G (5/5); total (10/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCTGTGGCTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCGGGCTGACCTGCTCGGCCAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTATCGCGGCAAAGCCAGTTCCACACCATGGCGTTGGTCAACCTGTCGGCGAAGATGATCGA > ACB122/627094‑627361 | ggCTGTGGCTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGGCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCGGGCTGACCTGCTc < 3:76509/136‑1 (MQ=255) cTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCCCACCGGGCGGCCCTGCGCGGCCCGcc > 3:168697/1‑136 (MQ=255) ggCGCTGACGGGAGCGCGGCGCGCTAGGGCACAATTGGCAGCGCCACATGGCGGGTCGAGCGCCCGGCGCTGACAATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCGGGCTGACCTGCGCGGCCAGCCggg < 4:168697/136‑1 (MQ=255) cgcTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCCCCCCCGGCGGGCCGGGGCGgcccgccccggg > 7:31452/1‑132 (MQ=255) tgtggggAGCGCGGCGCGGGTGGGCACCAAGGGCTTCGGCCCTTGTCTGGGCGAGCGCGCGGGGCTGACCATCCTGCAGACCGCGCACTGCCGGGGCCGGCACACCGGGCGGACCTGCGCGGCCAGCCCGGTGTTc < 8:193397/131‑1 (MQ=255) tGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCCCACCCGGCGGGCCTGCTGGGGCCGCCCGGGGttg > 6:51266/1‑135 (MQ=255) gcgcgAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTGACACCGGGCGGGCCGGCGCGGCCCGCCCGGGGGTGGGCCCGCCTGGCggc > 7:91861/1‑134 (MQ=255) gcgcgAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCCCACCCGGCGGACCAGCGCGGGCCGCCCCGTGGTGGCCCCGCCGGGCgcc > 2:136203/1‑134 (MQ=255) gAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGACCGAGCACTTCCGTGGCCGTCACACCGGGCGGCCCGGCTCCGCCCGCCCGGGGGTGGGCCCGCCTGGCGTCcggc > 2:82194/1‑136 (MQ=255) aCCAATGGCATCGGCACTTGTCTGGTCGAGCGCCAGGCGCTGACAATCCATCACAACGACCACTGCCGTGGCCGTCACACCGGGCTGACGTGCTCGGCCAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCgg < 3:110963/136‑1 (MQ=255) aTCGGCACATGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCCCACCCCGCGGCACTGCTCGGCCCGCCCCGGGTTGGCCCCCCCGGGCGGCCCGCTGGGGGGGGGCGACg > 1:68645/1‑136 (MQ=255) ggCACATGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCCGGCGGGCCTGCTCGGCCCGCCCGGGGTTCGACCCGCATGGCGCCCTGCTGGCGGTGCGCGCCgtgt > 4:62413/1‑136 (MQ=255) cacaTGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTGACACCCGGGGGGCCGGCGCGGGCAGCCCCGGGTTTGGCCCGCCTGGCGACCGGCTGGCGGGGCGCGCCGGGTcc > 4:145942/1‑136 (MQ=255) gAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTGACACCCGGCGGCCCTGGTAGGCCCGCCCGGGGGTGGGCCCGGCTGGCGGCCGGCGGGCGGTGCTCGCCGGGTGCTCTGCGCGCCCCg > 8:202797/1‑136 (MQ=255) gcgcTGACCATCCATCACAACGACCACTTCGGGGGCCGTCACACCGGGCTGACCTGCTCGGCCAGCCGGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTATcgc < 2:68645/136‑1 (MQ=255) taCCCTCCAGCACACCGCGCCCTCCCGTGGCGTTCACACGGGGCGTACTTGCGCGGCCTGCCCGGTGGTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTATCGCGGCaa < 8:90544/135‑1 (MQ=255) gACAATCCATCAGAACGAGCACTGCCGTGGCCGTCACACCGGGCTGACCTGCGCGGCCAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTATCGCGGCaa < 8:198161/136‑1 (MQ=255) aCCTTCAATCAGAACGCGCCCTGCCGGGGGCGTCACACCGGGCGGCCCTGCGGGCCCCGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTATCGCGGCaa < 6:201434/135‑1 (MQ=255) aCCATCCATCCGACCGAGCACTTCCGTGGCCGTCACACCGGGCTGACCTGCTCGGCCAGCCGGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTATCGCGGCaa < 1:28623/135‑1 (MQ=255) cTCGGCCAGACCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTATCGCGGCAAAGCCAGTTCCACACCATGGCGTTGGTCAACCTGTCGGCGAAGATGATCGa < 8:184933/135‑1 (MQ=255) | GGCTGTGGCTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCGGGCTGACCTGCTCGGCCAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTATCGCGGCAAAGCCAGTTCCACACCATGGCGTTGGTCAACCTGTCGGCGAAGATGATCGA > ACB122/627094‑627361 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |