Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,299,176 A→G 8.7% I65T (ATC→ACC)  livH_2 ← High‑affinity branched‑chain amino acidtransport system permease protein LivH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,299,1760AG 8.7% 134.2 / 4.4 46I65T (ATC→ACC) livH_2High‑affinity branched‑chain amino acidtransport system permease protein LivH
Reads supporting (aligned to +/- strand):  ref base A (19/23);  new base G (2/2);  total (21/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01

AATCAGCGGGATCAGACGGTTGCTGTTGCGCAGGGGGCGGTAGGCAACCCGTTCGATACTGTAGCCATAGGCACTGGTGACGAAGATCGTCGCGACGAAGGCGACGGTCATCAGGATCGGCAGCGAATGGATACCCATCATGGCCAGGCCCGCCAGGGCGATGAAGGCCACGTAGGAACCGATCATGTACACCTCGCCGTGGGCGAAGTTGATCATGCCAATGATGCCGTACACCATCGTGTAGCCGATGGCGATCAAGG  >  ACB122/1299046‑1299305
                                                                                                                                  |                                                                                                                                 
aaTCAGCGGGATCAGACGGTTGCTGTTGCGCAGGGGGCGGTAGGCAACCCGTTCGATACTGTAGCCATAGGCACTGGTGACGAAGATCGTCGCGACGAAGGCGACGGTCATCAGGATCGGCAGCGAATGGATAccc                                                                                                                              <  3:161440/136‑1 (MQ=255)
  tCAGCGGGATCAGACGGTTGCTGTTGCGCAGGGGGCGGTAGGCAACCCGTTCGATACTGTAGCCATAGGCACTGGTGACGAAGATCGTCGCGACGAAGGCGACGGTCATCAGGATCGGCAGCGAATGGATACCcat                                                                                                                            >  2:89132/1‑136 (MQ=255)
         gATCAGACGGTTGCTGTTGCGCAGGGGGCGGTAGGCAACCCGTTCGATACTGTAGCCATAGGCACTGGTGACGAAGATCGTCGCGACGAAGGCGACGGTCATCAGGATCGGCAGCGAATGGATACCCATCATGGcc                                                                                                                     <  4:25118/136‑1 (MQ=255)
         gATCAGACGGTTGCTGTTGCGCAGGGGGCGGTAGGCAACCCGTTCGATACTGTAGCCATAGGCACTGGTGACGAAGATCGTCGCGACGAAGGCGACGGTCATCAGGATCGGCAGCGAATGGATACCCATCATGGcc                                                                                                                     <  8:107682/136‑1 (MQ=255)
                  ggtGCTGTTGCGCAGGGGGCGGTAGGCAACCCGGTCGATACTGTAGCCATAGGCACTGGTGACGAAGATCGTCGCGACGAAGGCGACGGTCATCAGGATCGGCAGCGAATGGATACCCATCATGGCCAGGCCCGcc                                                                                                            <  4:40316/134‑1 (MQ=255)
                      cTGTTGCGCAGGGGGCGGTAGGCAACCCGTTCGATACTGTAGCCATAGGCACTGGTGACGAAGATCGTCGCGACGAAGGCGACGGTCATCAGGATCGGCAGCGAATGGATACCCATCATGGCCAGGCCCGCCAggg                                                                                                        >  3:60711/1‑136 (MQ=255)
                            cgcAGGGGGCGGTAGGCAACCCGTTCGATACTGTAGCCATAGGCACTGGTGACGAAGATCGTCGCGACGAAGGCGACGGTCATCAGGATCGGCAGCGAATGGATACCCATCATGGCCAGGCCCGCCAGGGCGATg                                                                                                   >  4:65123/1‑135 (MQ=255)
                             gcAGGGGGCGGTAGGCAACCCGTTCGATACTGTAGCCATAGGCACTGGTGACGAAGATCGTCGCGACGAAGGCGACGGTCATCAGGATCGGCAGCGAATGGATACCCATCATGGCCAGGCCCGCCAGGGCGATGaa                                                                                                 <  4:188786/136‑1 (MQ=255)
                                      ggTAGGCAACCCGTTCGATACTGTAGCCATAGGCACTGGTGACGAAGATCGTCGCGACGAAGGCGACGGTCATCAGGATCTGCAGCGAATGGATACCCATCATGGCCAGGCCCGCCAGGGCGATGAAGGCCACg                                                                                          >  7:25492/1‑134 (MQ=255)
                                        tAGGCAACCCGTTCGATACTGTAGCCATAGGCACTGGTGACGAAGATCGTCGCGACGAAGGCGACGGTCATCAGGATCGGCAGCGAATGGATACCCATCATGGCCAGGCCCGCCAGGGCGATGAAGGCCACGTAgg                                                                                      <  8:24385/136‑1 (MQ=255)
                                        tAGGCAACCCGTTCGATACTGTAGCCATAGGCACTGGTGACGAAGATCGTCGCGACGAAGGCGACGGTCATCAGGATCGGCAGCGAATGGATACCCATCATGGCCAGGCCCGCCAGGGCGATGAAGGCCACGTAgg                                                                                      <  4:98761/136‑1 (MQ=255)
                                        tAGGAAACCCTTTCGATACTGTATCCATAGCCACTGGTGACGAATATCGGCGAGACGACGGCGACGGTCATCAGGATCGGCAGCGAATGGATACCCATCATGGCCAGGCCCGCCAGGGCGATGAAGGCCACGTAgg                                                                                      <  3:124877/136‑1 (MQ=255)
                                          ggCAACCCGTTCGATACTGTAGCCATAGGCACTGGGGACGAAGATCGTCGCGACGAAGGCGACGGTCATCAGGATCGGCAGCGAATGGATACCCATCATGGCCAGGCCCGCCAGGGCGATGAAGGCCACGTAGGaa                                                                                    <  6:67536/136‑1 (MQ=255)
                                                ctgTTCGATACTGTAGCCATAGGCACTGGTGACGAAGATCGTCGCGACGAAGGCGACGGTCATCAGGATCGGCAGCGAATGGATACCCATCATGGCCAGGCCCGCCAGGGCGATGAAGGCCACGTAgg                                                                                      >  6:147434/3‑128 (MQ=255)
                                                ctgTTCGATACTGTAGCCATAGGCACTGGTGACGAAGATCGTCGCGACGAAGGCGACGGTCATCAGGATCGGCAGCGAATGGATACCCATCATGGCCAGGCCCGCCAGGGCGATGAAGGCCACGTAgg                                                                                      <  5:147434/126‑1 (MQ=255)
                                                ccGTTCGATACTGTAGCCATAGGCACTGGTGACGAAGATCGTCGCGACGAAGGCGACGGTCATCAGGATCGGCAGCGAATGGATACCCATCATGGCCAGGCCCGCCAGGGCGATGAAGGCCACGTAGGAACCGAt                                                                               >  6:193410/1‑135 (MQ=255)
                                                  gTTCGATACTGTAGCCATAGGCACTGGTGACGAAGATCGTCGCGACGAAGGCGACGGTCATCAGGATCGGCAGCGAATGGATACCCATCATGGCCAGGCCCGCCAGGGCGATGAAGGCCACGTAGGAACCGATCAt                                                                            >  4:154202/1‑136 (MQ=255)
                                                       aTACTGTAGCCATAGGCACTGGTGACGAAGATCGTCGCGACGAAGGCGACGGTCATCAGGATCGGCAGCGAATGGATACCCATCATGGCCAGGCCCGCCAGGGCGATGAAGGCCACGTAGGAACCGATCATGTaca                                                                       >  4:30399/1‑136 (MQ=255)
                                                          cTGTAGCCATAGGCACTGGTGACGAAGATCGTCGCGACGAAGGCGACGGTCATCAGGATCGGCAGCGAATGGGTACCCATCATGGCCAGGCCCGCCAGGGCGATGAAGGCCACGTAGGAACCGATCATGTACACCt                                                                    >  1:200175/1‑136 (MQ=255)
                                                          cTGTAGCCATAGGCACTGGTGACGAAGATCGTCGCGACGAAGGCGACGGTCATCAGGATCGGCAGCGAATGGGTACCCATCATGGCCAGGCCCGCCAGGGCGATGAAGGCCACGTAGGAACCGATCATGTACACCt                                                                    >  1:197661/1‑136 (MQ=255)
                                                               gCCATAGGCACTGGTGACGAAGATCGTCGCGACGAAGGCGACGGTCATCAGGATCGGCAGCGAATGGATACCCATCATGGCCAGGCCCGCCAGGGCGATGAAGGCCACGTAGGAACCGATCATGTACACCTCGCCg                                                               >  1:27285/1‑136 (MQ=255)
                                                                     ggCACTGGTGACGAAGATCGTCGCGACGAAGGCGACGGTCATCAGGATCGGCAGCGAATGGATACCCATCATGGCCAGGCCCGCCAGGGCGATGAAGGCCACGTAGGAACCGATCATGTACACCTCGCCGTGGGc                                                          >  2:55791/1‑135 (MQ=255)
                                                                        aCTGGTGACGAAGATCGTCGCGACGAAGGCGACGGTCATCAGGATCGGCAGCGAATGGATACCCATCATGGCCAGGCCCGCCAGGGCGATGAAGGCCACGTAGGAACCGATCATGTACACCTCGCCGTGGGCGAAg                                                      >  5:101746/1‑136 (MQ=255)
                                                                        aCTGGTGACGAAGATCGTCGCGACGAAGGCGACGGTCATCAGGATCGGCAGCGAATGGATACCCATCATGGCCAGGCCCGCCAGGGCGATGAAGGCCACGTAGGAACCGATCATGTACACCTCGCCGTGGGCGAAg                                                      >  4:199592/1‑136 (MQ=255)
                                                                           ggTGACGAAGATCGTCGCGACGAAGGCGACGGTCATCAGGATCGGCAGCGAATGGATACCCATCATGGCCAGGCCCGCCAGGGCGATGAAGGCCACGTAGGAACCGATCATGTACACCTCGCCGTGGGCGAAGtt                                                    >  4:159335/1‑135 (MQ=255)
                                                                             tcaCGAACATCGTCGCGACGAAGGCGACGGACATCAGGATCGGCAGCGAATGCAAACCCATCAAGCCCAGCCCCGCCAGGCCGATCAAGGCCACGTACGAAACGATCATGTACACCTCGCCCTGGGCGAAg                                                      >  8:132074/3‑131 (MQ=255)
                                                                             tGACGAAGATCGTCGCGACGAAGGCGACGGTCATCAGGATCGGCAGCGAATGGATACCCATCATGGCCAGGCCCGCCAGGGCGATGAAGGCCACGTAGGAACCGATCATGTACACCTCGCCGTGGGCGAAGTTGa                                                  >  1:56697/1‑135 (MQ=255)
                                                                             tGACGAAGATCGTCGCGACGAAGGCGACGGTCATCAGGATCGGCAGCGAATGGATACCCATCATGGCCAGGCCCGCCAGGGCGATGAAGGCCACGTAGGAACCGATCATGTACACCTCGCCGTGGGCGAAGTTGAt                                                 >  6:12468/1‑136 (MQ=255)
                                                                             tGACGAAGATCGTCGCGACGAAGGCGACGGTCATCAGGATCGGCAGCGAATGGATACCCATCATGGCCAGGCCCGCCAGGGCGATGAAGGCCACGTAGGAACCGATCATGTACACCTCGCCGTGGGCGAAGTTGAt                                                 >  1:111115/1‑136 (MQ=255)
                                                                             tGACGAAGATCGTCGCGACGAAGGCGACGGTCATCAGGATCGGCAGCGAATGGATACCCATCATGGCCAGGCCCGCCAGGGCGATGAAGGCCACGTAGGAACCGATCATGTACACCTCGCCGTGGGCGAAGTTGAt                                                 >  4:203648/1‑136 (MQ=255)
                                                                                    gagcgtcgCGACGAAGGCGACGGTCATCAGGATCTGCAGAGAATGGATACCAATCATGGCCAGGCCCGCCAGGGAGATGAATTCCACGTAGGAACCGATCATGTACACCTCGCCGTGGGCGAAGTTGATCATGCCa                                          <  7:20960/133‑1 (MQ=38)
                                                                                    gATCGTCGCGACGAAGGCGACGGTCATCTGGATCGGGAGCGAATGGATTCCCATCATGGCCAGGCCCGCCAGGGCGATGAAGGACACGTAGGAACCGATCATGTACACCTCGCCGTGGGCGAAGTTGATCATGCCa                                          <  1:107363/136‑1 (MQ=255)
                                                                                    gATCGGCGCGACGAAGGCGACTGTCATCAGGATCGGCAGCGAATGGATACGCATCATGGCCAGGCCCGCCAGGGCGATGAAGGCCACGTAGGAACCGATCATGTACACCTCGCCGTGGGCGAAGTTGATCATGCCa                                          <  8:4189/136‑1 (MQ=255)
                                                                                    gATCGGCGCGACGAAGGCGACGGTCATCAGGATCGGCAGCGAATGGATACCCATCATGGCCAGGCCCGCCAGGGCGATGAAGGCCACGTAGGAACCGATCATGTACACCTCGCCGTGGGCGAAGTTGATCATGCCa                                          <  4:108611/136‑1 (MQ=255)
                                                                                       cgtcgCGACGAAGGCGACGGTCATCAGGATCGGCAGCGAATGGATACCCATCATGGCCAGGCCCGCCAGGGCGATGAAGGCCACGTAGGAACCGATCATGTACACCTCGCCGTGGGCGAAGTTGATCATGCCAatg                                       <  1:89132/136‑1 (MQ=255)
                                                                                        gtcgCGACGAAGGCGACGGTCATCAGGATCGGCAGCGAATGGATACCCATCATGGCCAGGCCCGCCAGGGCGATGAAGGCCACGTAGGAACCGATCATGTACACCTCGCCGTGGGCGAAGTTGATCATGCCAatg                                       <  6:101746/135‑1 (MQ=255)
                                                                                          cgcgACGAAGGCGACGGTCATCAGGATCGGCAGCGAATGGATACCCATCATGGCCAGGCCCGCCAGGGCGATGAAGGCCACGTAGGAACCGATCATGTACACCTCGCCGTGGGCGAAGTTGATCATGCCAatgatg                                    <  7:12424/136‑1 (MQ=255)
                                                                                            cgacgaAGGCGACGGTCATCAGGATCGGCAGCGAATGGATACCCATCATGGCCAGGCCCGCCAGGGCGATGAAGGCCACGTAGGAACCGATCATGTACACCTCGCCGTGGGCGAAGTTGATCATGCCAATGATGcc                                  <  3:199592/136‑1 (MQ=255)
                                                                                                 aaGGCGACGGTCATCAGGATCGGCAGCGAATGGATACCCATCATGGCCAGGCCCGCCAGGGCGATGAAGGCCACGTAGGAACCGATCATGTACACCTCGCCGTGGGCGAAGTTGATCATGCCAATGATGCCGTaca                             <  2:56697/136‑1 (MQ=255)
                                                                                                      gACGGTCATCAGGATCGGCAGCGAATGGGTACCCATCATGGCCAGGCCCGCCAGGGCGATGAAGGCCACGTAGGAACCGATCATGTACACCTCGCCGTGGGCGAAGTTGATCATGCCAATGATGCCGTACACCATc                        <  2:200175/136‑1 (MQ=255)
                                                                                                      gACGGTCATCAGGATCGGCAGCGAATGGGTACCCATCATGGCCAGGCCCGCCAGGGCGATGAAGGCCACGTAGGAACCGATCATGTACACCTCGCCGTGGGCGAAGTTGATCATGCCAATGATGCCGTACACCATc                        <  2:197661/136‑1 (MQ=255)
                                                                                                                 ggATCGGCAGCGAATGGATACCCATCATGGCCAGGCCCGCCAGGGCGATGAAGGCCACGTAGGAACCGATCATGTACACCTCGCCGTGGGCGAAGTTGATCATGCCAATGATGCCGTACACCATCGTGTAGCCGAt             >  2:199305/1‑136 (MQ=255)
                                                                                                                    tCGGCAGCGAATGGATACCCATCATGGCCAGGCCCGCCAGGGCGATGAAGGCCACGTAGGAACCGATCATGTACACCTCGCCGTGGGCGAAGTTGATCATGCCAATGATGCCGTACACCATCGTGTAGCCGATGGc          <  3:30399/136‑1 (MQ=255)
                                                                                                                     cGGCAGCGAATGGATACCCACCCTGGCCAGGCCCGCCAGGGCGAGGAAGGCCACGTAGGAACCGATCATGTACACCTCGCCGTGGGCGAAGTTGATCATGCCAATGATGCCGTACACCATCGTGTAGCCGATGGCg         <  1:28606/136‑1 (MQ=255)
                                                                                                                      ggCAGCGAATGGATACCCATCATGGCCAGGCCCGCCAGGGCGATGAAGGCCACGTAGGAACCGATCATGTACACCTCGCCGTGGGCGAAGTTGATCATGCCAATGATGCCGTACACCATCGTGTAGCCGATGGc          <  1:128153/134‑1 (MQ=255)
                                                                                                                            gAATGGATACCCATCATGGCCAGGCCCGCCAGGGCGATGAAGGCCACGTAGGAACCGATCATGTACACCTCGCCGTGGGCGAAGTTGATCATGCCAATGATGCCGTACACCATCGTGTAGCCGATGGCGATCAAgg  <  4:60711/136‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                 
AATCAGCGGGATCAGACGGTTGCTGTTGCGCAGGGGGCGGTAGGCAACCCGTTCGATACTGTAGCCATAGGCACTGGTGACGAAGATCGTCGCGACGAAGGCGACGGTCATCAGGATCGGCAGCGAATGGATACCCATCATGGCCAGGCCCGCCAGGGCGATGAAGGCCACGTAGGAACCGATCATGTACACCTCGCCGTGGGCGAAGTTGATCATGCCAATGATGCCGTACACCATCGTGTAGCCGATGGCGATCAAGG  >  ACB122/1299046‑1299305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑