| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 1,375,132 | C→G | 9.3% | A277G (GCC→GGC) | dinB → | DNA polymerase IV |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 1,375,132 | 0 | C | G | 9.3% | 130.1 / 7.0 | 43 | A277G (GCC→GGC) | dinB | DNA polymerase IV |
| Reads supporting (aligned to +/- strand): ref base C (20/19); new base G (2/2); total (22/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.37e-01 | |||||||||||
GTGCGGTGCATAACGACAGCCGACGCCAGTCGGTGAGTGTGGAAAACACCTACGACACCGACCTGCCAGACCTGGCCAGTTGCCTGGCGCGGCTGCCAGAGTTGCTGGATAGCCTGAACGAACGCATCGCCCGAATGGACAGCAGTTACCGGCCAGACAAACCGTTCGTCAAGGTCAAGTTCCATGACTTCAGCCAGACCACCATGGAGCAGGCCGGGGCGGGCCGGGACCTGGAGAGTTATCGGCAATTGCTGGG > ACB122/1375003‑1375258 | gTGCGGTGCATAACGACAGCCGACGCCAGTCGGTGAGTGTGGAAAACACCTACGACACCGACCTGCCAGACCTGGCCAGTTGCCTGGCGCGGCTGCCAGAGTTGCTGGATAGCCTGAACGAACGCATCGCCCGAAt < 7:172061/136‑1 (MQ=255) ggCGGTGCATAACGACAGCCGACGCCAGTCGGTGAGTGTGGAAAACACCTACGACACCGACCTGCCAGACCTGGCCAGTTGCCTGGCGCGGCTGCCAGAGTTGCTGGATAGCCTGAACGAACGCATCGCCCGAATg < 6:90718/135‑1 (MQ=255) ccGACGCCAGTCGGTGAGTGTGGAAAACACCTACGACACCGACCTGCCAGACCTGGCCAGTTGCCTGGCGCGGCTGCCAGAGTTGCTGGATAGCCTGAACGAACGCATCGCCCGAATGGACAGCAGTTACCGGc > 4:153099/1‑134 (MQ=255) ccGACGCCAGTCGGTGAGTGTGGAAAACACCTACGACACCGACCTGCCAGACCTGGCCAGTTGCCTGGCGCGGCTGCCAGAGTTGCTGGATAGCCTGAACGAACGCATCGCCCGAATGGACAGCAGTTACCGGc > 2:144870/1‑134 (MQ=255) ccGACGCCAGTCGGTGAGTGTGGAAAACACCTACGACACCGACCTGCCAGACCTGGCCAGTTGCCTGGCGCGGCTGCCAGAGTTGCTGGATAGCCTGAACGAACGCATCGCCCGAATGGACAGCAGTTACCGGc > 8:168717/1‑134 (MQ=255) ccGACGCCAGTCGGTGAGTGTGGAAAACACCTACGACACCGACCTGCCAGACCTGGCCAGTTGCCTGGCGCGGCTGCCAGAGTTGCTGGATAGCCTGAACGAACGCATCGCCCGAATGGACAGCAGTTACCGGc > 5:182549/1‑134 (MQ=255) ccGACGCCAGTCGGTGAGTGTGGAAAACACCTACGACACCGACCTGCCAGACCTGGCCAGTTGCCTGGCGCGGCTGCCAGAGATGCTGGATAGCCTGAACGAACGCCTCGCCCGAATGGACAGCAGGTACCGGc > 5:166824/1‑134 (MQ=255) cGACGCCAGTCGGTGAGTGTGGAAAACACCTACGACACCGACCTGCCAGACCTGGCCAGTTGCCTGGCGCGGCTGCCAGAGTTGCTGGATAGCCTGAACGAACGCATCGCCCGAATGGACAGCAGTTACCGGCCAg < 1:45210/136‑1 (MQ=255) gACGCCAGTCGGTGAGTGTGGAAAACACCTACGACACCGACCTGCCAGCCCTGGCCAGTTGCCTGGCGCGGCTGCCAGAGTTGCTGGATAGCCTGAACGAACGCATCGCCCGAATGGACAGCAGTTACCGGCCAGa > 1:105557/1‑136 (MQ=255) gACGCCAGTCGGTGAGTGTGGAAAACACCTACGACACCGACCTGCCAGACCTGGCCAGTTGCCTGGCGCGGCTGCCAGAGTTGCTGGATAGCCTGAACGAACGCATCGGCCGAATGGACAGCAGTTACCGGCCAGa > 4:38851/1‑136 (MQ=255) gACGCCAGTCGGTGAGTGTGGAAAACACCTACGACACCGACCTGCCAGACCTGGCCAGTTGCCTGGCGCGGCTGCCAGAGTTGCTGGATAGCCTGAACGAACGCATCGGCCGAATGGACAGCAGTTACCGGCCAGa > 4:36005/1‑136 (MQ=255) gACGCCAGTCGGTGAGTGTGGAAAACACCTACGACACCGACCTGCCAGACCTGGCCAGTTGCCTGGCGCGGCTGCCAGAGTTGCTGGATAGCCTGAACGAACGCATCGCCCGAATGGACAGCAGTTACCGGCCAGa > 6:79293/1‑136 (MQ=255) gTGAGTGTGGAAAACACCTACGACACCGACCTGCCAGACCTGGCCAGTTGCCTGGCGCGGCTGCCAGAGTTGCTGGATAGCCTGAACGAACGCATCGCCCGAATGGACAGCAGTTACCGGCCAGACAAACCGTTCg < 1:147440/136‑1 (MQ=255) tgGAAAACACCTACGACACCGACCTGCCAGACCTGGCCAGTTGCCTGGCGCGGCTGCCAGAGTTGCTGGATAGCCTGAACGAACGCATCGGCCGAATGGACAGCAGTTACCGGCCAGACAAACCGTTCgtcaaggt < 3:38851/136‑1 (MQ=255) tgGAAAACACCTACGACACCGACCTGCCAGACCTGGCCAGTTGCCTGGCGCGGCTGCCAGAGTTGCTGGATAGCCTGAACGAACGCATCGGCCGAATGGACAGCAGTTACCGGCCAGACAAACCGTTCgtcaaggt < 3:36005/136‑1 (MQ=255) aaaaCACCTACGACACCGACCTGCCAGACCTGGCCAGTTGCCTGGCGCGGCTGCCAGAGTTGCTGGATAGCCTGAACGAACGCATCGCCCGAATGGACAGCAGTTACCGGCCAGACAAACCGTTCgtcaaggtc > 2:193953/1‑134 (MQ=255) tACGACACCGACCTGCCAGACCTGGCCAGTTGCCTGGCGCGGCTGCCAGAGTTGCTGGATAGCCTGAACGAACGCATCGCCCGAATGGACAGCAGTTACCGGCCAGACAAACCGTTCGTCAAGGTCAAGTTCCAt > 8:39795/1‑135 (MQ=255) cacCGACCTGCCAGACCTGGCCAGTTGCCTGGCGCGGCTGCCAGAGTTGCTGGATAGCCTGAACGAACGCATCGCCCGAATGGACAGCAGTTACCGGCCAGACAAACCGTTCGTCAAGGTCAAGTTCCATGACtt > 2:15211/1‑135 (MQ=255) aCCTGCCAGACCTGGCCAGTTGCCTGGCGCGGCTGCCAGAGTTGCTGGATAGCCTGAACGAACGCATCGCCCGAATGGACAGCAGTTACCGGCCAGACAAACCGTTCGTCAAGGTCAAGTTCCATGACTTCAGCCa > 4:151291/1‑136 (MQ=255) aCCTGCCAGACCTGGCCAGTTGCCTGGCGCGGCTGCCAGAGTTGCTGGATAGCCTGAACGAACGCATCGCCCGAATGGACAGCAGTTACCGGCCAGACAAACCGTTCGTCAAGGTCAAGTTCCATGACTTCAGCCa < 7:170492/136‑1 (MQ=255) aCCTGCCAGACCTGGCCAGTTGCCTGGCGCGGCTGCCAGAGTTGCTGGATAGCCTGAACGAACGCATCGCCCGAATGGACAGCAGTTACCGGCCAGACAAACCGTTCGTCAAGGTCAAGTTCCATGACTTCAGCCa < 5:35844/136‑1 (MQ=255) aCCTGCCAGACCTGGCCAGTTGCCTGGCGCGGCTGCCAGAGTTGCTGGATAGCCTGAACGAACGCATCGCCCGAATGGACAGCAGTTACCGGCCAGACAAACCGTTCGTCAAGGTCAAGTTCCATGACTTCAGCCa < 1:91902/136‑1 (MQ=255) ccAGACCTGGCCAGTTGCCTGGCGCGGCTGCCAGAGTTGCTGGATAGCCTGAACGAACGCATCGCCCGAATGGACAGCAGTTACCGGCCAGACAAACCGTTCGTCAAGGTCAAGTTCCATGACTTCAGCCAGacca < 1:15211/136‑1 (MQ=255) ccAGACCTGGCCAGTTGCCTGGCGCGGCTGCCAGAGTTGCTGGATAGCCTGAACGAACGCATCGCCCGAATGGACAGCAGTTACCGGCCAGACAAACCGTTCGTCAAGGTCAAGTTCCATGACTTCAGCCAGacca < 7:7548/136‑1 (MQ=255) ccAGACCTGGCCAGTTGCCTGGCGCGGCTGCCAGAGTTGCTGGATAGCCTGAACGAACGCATCGCCCGAATGGACAGCAGTTACCGGCCAGACAAACCGTTCGTCAAGGTCAAGTTCCATGACTTCAGCCAGacca < 7:105636/136‑1 (MQ=255) ccTGGCCAGTTGCCTGGCGCGGCTGCCAGAGTTGCTGGATAGCCTGCACGCACGCATCGCCCGAATGGACAGCAGTTACCGGCCAGACAAACCGGTCGTCAAGGTCAAGTTCCATGACTTCAGCCCGACCACCATg < 1:123074/136‑1 (MQ=255) cAGTTGCCTGGCGCGGCTGCCAGAGTTGCTGGATAGCCTGAACGAACGCATCGCCCGAATGGACAGCAGTTACCGGCCAGACAAACCGTTCGTCAAGGTCAAGTTCCATGACTTCAGCCAGACCACCATGGAGCAg > 8:148216/1‑136 (MQ=255) gCCTGGCGCGGCTGCCAGAGTTGCGGGAGAGCCGGAACGAACGCATCGCCCGAAGGGACAGCTGTAACCGGCCAGACAAACCGCTCGTCAATGTCACGTTCCATGACTTCAGCCAGACCACCATGGAGCAGGCCg < 1:181928/135‑1 (MQ=255) gCCGGGCGCGGCTGCCAGAGTGGCTGGATAGCCTGAACGAACGCATCGCCCGAATGGACAGCAGTTACCGGCCAGACAAACCGTTCGTCAAGGTCAAGTTCCATGACTTCAGCCAGACCACCATGGAGCAGGCCg < 5:36250/135‑1 (MQ=255) cgcgGCTGCCAGAGTTGCTGGATAGCCTGAACGAACGCATCGCCCGAATGGACAGCAGTTACCGGCCAGACAAACCGTTCGTCAAGGTCAAGTTCCATGACTTCAGCCAGACCACCATGGAGCAGGCCGgggcggg > 3:167814/1‑136 (MQ=255) gcgGCTGCCAGAGTTGCGGGATAGCCTGCACGAACGCATCGCCCGAATGGACAGCAGTTACCGGCCAGACAAACCGTTCGTCAAGGTCAAGTTCCATGACTTCAGCCAGACCACCATGGAGCAGGCCGgggcgggc < 1:95123/136‑1 (MQ=255) ggCTGCCAGAGTTGCTGGATAGCCTGAACGAACGCATCGCCCGAATGGACAGCAGTTa > 1:20115/1‑58 (MQ=255) ggCTGCCAGAGTTGCTGGATAGCCTGAACGAACGCATCGCCCGAATGGACAGCAGTTa > 5:118964/1‑58 (MQ=255) ggCTGCCAGAGTTGCTGGATAGCCTGAACGAACGCATCGCCCGAATGGACAGCAGTTa < 2:20115/58‑1 (MQ=255) ggCTGCCAGAGTTGCTGGATAGCCTGAACGAACGCATCGCCCGAATGGACAGCAGTTa < 6:118964/58‑1 (MQ=255) tGGATAGCCTGAACGAACGCATCGCCCGAATGGACAGCAGTTACCGGCCAGACAAACCGTTCGTCAAGGTCAAGTTCCATGACTTCAGCCAGACCACCATGGAGCAGGCCGGGGCGGGCCGGGACCTGGAGAGTTa > 8:34392/1‑136 (MQ=255) cTGAACGAACGCATCGCCCGAATGGACAGCAGTTACCGGCCAGACAAACCGTTCGTCAAGGTCAAGTTCCATGACTTCAGCCAGACCACCATGGAGCAGGCCGGGGCGGGCCGGGACCTGGAGAGTTATCGGCAAt < 5:79293/136‑1 (MQ=255) cTGAACGAACGCATCGCCCGAATGGACAGCAGTTACCGGCCAGACAAACCGTTCGTCAAGGGCAAGTTCCATGACTTCAGCCAGACCACCATGGAGCAGGCCGGGGCGGGCCGGGACCTGGAGAGTTATCGGCAAt < 3:155579/136‑1 (MQ=255) gaacgaacGCATCGCCCGAATGGACAGCAGTTACCGGCCAGACAAACCGTTCGTCAAGGACAAGTTCCATGACTTCAGCCAGACCACCATGGAGCAGGCCGGGGCGGGCCGGGACCTGGAGAGTTATCGGCAATTg > 1:60691/1‑136 (MQ=255) aacgCATCGCCCGAATGGACAGCAGTTACCGGCCAGACAAACCGTTCGTCAAGGTCAAGTTCCATGACTTCAGCCAGACCACCATGGAGCAGGCCGGGGCGGGCCGGGACCTGGAGAGTTATCGGCAATTGCTggg > 4:163959/1‑136 (MQ=255) aacgCATCGCCCGAATGGACAGCAGTTACCGGCCAGACAAACCGTTCGTCAAGGTCAAGTTCCATGACTTCAGCCAGACCACCATGGAGCAGGCCGGGGCGGGCCGGGACCTGGAGAGTTATCGGCAATTGCTggg > 8:150973/1‑136 (MQ=255) aTCGCCCGAATGGACAGCAGTTACCGGCCAGACAAACCGTTCGTCAAGGTCAAGTTCCATGACTTCAg < 7:114801/68‑1 (MQ=255) aTCGCCCGAATGGACAGCAGTTACCGGCCAGACAAACCGTTCGTCAAGGTCAAGTTCCATGACTTCAg > 8:114801/1‑68 (MQ=255) | GTGCGGTGCATAACGACAGCCGACGCCAGTCGGTGAGTGTGGAAAACACCTACGACACCGACCTGCCAGACCTGGCCAGTTGCCTGGCGCGGCTGCCAGAGTTGCTGGATAGCCTGAACGAACGCATCGCCCGAATGGACAGCAGTTACCGGCCAGACAAACCGTTCGTCAAGGTCAAGTTCCATGACTTCAGCCAGACCACCATGGAGCAGGCCGGGGCGGGCCGGGACCTGGAGAGTTATCGGCAATTGCTGGG > ACB122/1375003‑1375258 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |