| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 1,497,924 | C→T | 13.3% | G191G (GGC→GGT) | petA → | Ubiquinol‑cytochrome c reductase iron‑sulfursubunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 1,497,924 | 0 | C | T | 13.3% | 60.6 / 5.1 | 31 | G191G (GGC→GGT) | petA | Ubiquinol‑cytochrome c reductase iron‑sulfursubunit |
| Reads supporting (aligned to +/- strand): ref base C (12/14); new base T (2/2); total (15/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.59e-01 | |||||||||||
GCCCGTGCCACGGCTCTCACTATGACCTGGCTGGTCGTGTCTACAAGTCCCAGCCGGCGCCTCTCAACCTGCCCGTGCCACCGCACTCTTATGAGTCGGACGAGATCATCGTCATTGGCGTCGACCAGGAGAACGCATGATGAGCAAGTTCATGGACTGGATTGATGCTCGCTTCCCCGCCACCAAGATGTGGGAAGACCACCTCAGCAAGTATTACGCACCCAAGAACTTCAACTTCCTGTACTTCTT > ACB122/1497806‑1498054 | gCCCGTGCCACGGCTCTCACTATGACCTGGCTGGTCGTGTCTACAAGTCCCAGCCGGCGCCTCTCAACCTGCCCGTGCCACCGCACTCTTATGAGTCGGACGAGATCATCGTCATTGGCGTCGACCAGGAGAACg > 1:5705/1‑135 (MQ=255) cccTTGCAACGGCTCTCACTATGACCTGGCTGGTCGTGTCTACAATTCCGAGCCGGCGCCTCTCAACCTGCCCGTGCCACCGCAATCTTATGAGTCGGACGAGATCATCGTCATTGGCGTCGACCATGAGAACGc < 1:102905/135‑1 (MQ=255) cccGTGCCACGGCTCTCACTATGACCTGGCTGGTCGTGTCTACAAGTCCCAGCCGGCGCCTCTCAACCTGCCCGTGCCACCGCACTCTTATGAGTCGGACGAGATCATCGTCATTGGCGTCGACCAGGAGAACGc < 5:203037/135‑1 (MQ=255) ccACGGCTCTCACTATGACCTGGCTGGTCGTGTCTACAAGTCCCAGCCGGCGCCTCTCAACCTGCCCGTGCCACCGCACTCTTATGAGTCGGCCGAGTTCATCGTGATTGGCGGGGACCAGGAGACCGCatgat > 5:119944/1‑134 (MQ=255) ggctggTCGTGTCTACAAGTCCCAGCCGGCGCCTCTCAACCTGCCCGTGCCACCGCACTCTTATGAGTCGGACGAGATCATCGTCATTGGCGTCGACCAGGAGAACGCATGATGAGCAAGTTCATGGACTGGAtt > 6:104864/1‑135 (MQ=255) tggTCGTGTCTACAAGTCCCAGCCGGCGCCTCTCAACCTGCCCGTGCCACCGCACTCTTATGAGTCGGACGAGATCATCGTCATTGGTGTCGACCAGGAGAACGCATGATGAGCAAGTTCATGGCCTGGATTGAtt > 6:107607/1‑135 (MQ=255) tggTCGTGTCTACAAGTCCCAGCCGGCGCCTCTCAACCTGCCAGTGCCACCGCACTCTTATGAGTCGGACGAGATCATAGTCATTGGTGTGGCCCTGGACAACGCCAGATGAGCACGTTCATGGACTGGATTGATg > 6:110089/1‑136 (MQ=255) tggTCGTGTCTACAAGTCCCAGCCGGCGCCTCTCAACCAGCCCGTGCCACCGCACTCTTATGAGTCGGACGAGATCATCGTCATTGGCGTCGACCAGGAGAACGCATGATGAGCAAGTTCATGGACTGGATTGCTg > 3:57249/1‑136 (MQ=255) tCTACAAGTCCCAGCCGGCGCCTCTCAACCTGCCCGTGCCACCGCACTCTTATGAGTCGGACGAGATCATCGTCATTGGCGTCGACCAGGAGAACGCATGATGAGCAAGTTCATGGACTGGATTGATGCTCGCtt < 2:5705/135‑1 (MQ=255) aCAAGTCCCAGCCGGCGCCTCTCAACCTGCCCGTGCCACCGCACTCTTATGAGTCGGACGAGATCATCGTCATTGGCGTCGCCCAGGAGAACGCATGATGAGCAAGTTCATGGACTGGATTGATGCACGCTTcccc > 2:111966/1‑136 (MQ=255) ctctCAACCTGCCCGTGCCACCGCACTCTTATGAGTCGGACGAGATCAGCGTCATTGGCGTCGACCAGGAGAACGCATGATGAGCAAGTTCATGGACTGGATTGATGCTCGCTTCCCCGCCACCAAGATGTGGGaa < 2:33470/136‑1 (MQ=255) aaCCTGCCCGTGCCACCGCACTCTTATGAGTCGGACGAGATCATCGTCTTTGGCGTCGACCAGGAGAACGCATGATGAGCAAGTTCATGGACTGGATTGATGCTCGCTTCCCCGCCACCAAGATGTGGGAAGac < 4:58900/134‑1 (MQ=255) aaCCTGCCCGTGCCACCGCACTCTTATGAGTCGGACGAGATCATCGTCATTGGCGTCGACCAGGAGAACGCATGATGAGCAAGTTCATGGACTGGATTGATGCTCGCTTCCCCGCCACCAAGATGTGGGAAGacca > 5:92678/1‑136 (MQ=255) cccGTGCCACCGCACTCTTATGAGTCGGACGAGATCATCGTCATTGGCGTCGACCAGGAGAACGCATGATGAGCAAGTTCATGGACTGGATTGATGCTCGCTTCCCCGCCACCAAGATGTGGGAAGACCACCTc < 1:154153/134‑1 (MQ=255) cccGTGCCACCGCACTCTTATGAGTCGGACGAGATCATCGTCATTGGCGTCGACCAGGAGAACGCATGATGAGCAAGTTCATGGACTGGATTGATGCTCGCTTCCCCGCCACCAAGATGTGGGAAGACCACCTCAg < 4:118567/136‑1 (MQ=255) cGTGCCACCGCACTCTTATGAGTCGGACGAGATCATCGTCATTGGCGTCGACCAGGAGAACGCATGATGAGCAAGTTCATGGACTGGATTGATGCTCGCTTCCCCGCCACCAAGATGTGGGAAGACCACCTCAGc > 5:97700/1‑135 (MQ=255) tGCCACCGCACTCTTATGAGTCGGACGAGATCATCGTCATTGGAGTCGACCAGGAGAACGCATGATGAGCAAGTTCATGGACTGGATTCATGCTCGCTTCCCCGCCACCAAGATGTGGGAAGACCACCTCAGCAAg > 1:102547/1‑136 (MQ=255) ccACCGCACTCTTATGCGTCGGACGAGATCATCTTCATTGGCGTCGACCAGGAGAACGCATGATGAGCAAGTTCATGGACTGGATTGATGCTCGCTTCCCCGCCACCAAGATGTGGGAAGACCACCTCAGCAAGTa < 6:5500/136‑1 (MQ=255) cACCGCACTCTTATGAGTCGGACGAGATCATCGTCATTGGCGTCGACCAGGAGAACGCATGATGAGCAAGTTCATGGACTGGATTGATGCTCGCTTCCCCGCCACCAAGATGTGGGAAGACCACCTCAGCAAGTAt > 3:9537/1‑136 (MQ=255) aCCGCACTCTTATGAGTCGGACGAGATCATCGTCATTGGCGTCGACCAGGAGAACGCATGATGAGCAAGTTCATGGACTGGATTGATGCTCGCTTCCCCGCCACCAAGATGTGGGAAGACCACCTCAGCAAGTAtt < 1:25244/136‑1 (MQ=255) aCCGCACTCTTATGAGTCGGACGAGATCATCGTCATTGGCGTCGACCAGGAGAACGCATGATGAGCAAGTTCATGGACTGGATTGATGCTCGCTTCCCCGCCACCAAGATGTGGGAAGACCACCTCAGCAAGTAtt < 6:119944/136‑1 (MQ=255) ccGCTCTCTTATGAGTCGGACGAGATCATCGTCATTGGCGTCGACCAGGAGAACGCATGATGAGCAAGTTCATGGACTGGATTGATGCTCGCTTCCCCGCCACCAAGATGTGGGAAGACCACCTCAg < 1:131740/127‑1 (MQ=255) ccGCACTCTTATGAGTCGGACGAGATCATCGTCATTGGCGTCGACCAGGAGAACGCATGATGAGCAAGTTCATGGACTGGATTGATGCTCGCTTCCCCGCCACCAAGATGTGGGAAGACCACCTCAg > 2:131740/1‑127 (MQ=255) ggACGAGATCATCGTCATTGGCGTCGACCAGGAGAACGCATGATGAGCAAGTGCATGGACTGGATTGATGCTCGCTTCCCCGCCACCAAGATGTGGGAAGACCACCTCAGCAAGTATTACGCACCCAAGaacttca < 4:160253/136‑1 (MQ=255) ggACGAGATCATCGTCATTGGCGGCGAACAGGAGAACGCATGATGAGCAAGTTCATGGACTGGATTGATGCTCGCTTCCCCGCCACCAAGGTGTGGGAAGACCACCTCAGCAAGTATTACGCACCCTAGaacttca < 2:152964/136‑1 (MQ=255) gagaTCATCGTCATTGGCGTCGACCAGGAGAACGCATGATGAGCAAGTTCATGGACTGGATTGATGCTCGCTTCCCCGCCACCAAGATGTGGGAAGACCACCTCAGCAAGTATTACGCACCCAAGaacttcaactt > 1:9465/1‑136 (MQ=255) gagaTCATCGTCATTGGCGTCGACCAGGAGAACGCATGATGAGCAAGTTCATGGACTGGATTGATGCTCGCTTCCCCGCCACCAAGATGTGGGAAGACCACCTCAGCAAGTATTACGCACCCAAGaact > 7:73057/1‑129 (MQ=255) gagaTCATCGTCATTGGCGTCGACCAGGAGAACGCATGATGAGCAAGTTCATGGACTGGATTGATGCTCGCTTCCCCGCCACCAAGATGTGGGAAGACCACCTCAGCAAGTATTACGCACCCAAGaact < 8:73057/129‑1 (MQ=255) cATTGGCGTCGACCAGGAGAACGCATGATGAGCAAGTTCATGGACTGGATTGATGCTCGCTTCCCCGCCACCAAGATGTGGGAAGACCACCTCAGCAAGTATTACGCACCCAAGAACTTCAACTTCCTGTActtct > 2:20016/1‑136 (MQ=255) aTTGGTGTCGACCAGGATAACGCATGATGAGCAAGTTCATGGACTGGATTGATGCTCGCTTCCCCGCCACCAAGATGTGGGAAGACCACCTCAGCAAGTATTACGCACCCAAGAACTTCAACTTCCTGTActtctt < 5:110089/136‑1 (MQ=255) aTTGGTGACGACCATGAGAACGCATGATGACCAAGTTCATGGACTGGATTGATGCTCGCTTCCCCGCCACCAAGATGTGGGAAGACCACCTCAGCAAGTATTACGCACCCAAGAACTTCAACTTCCTGTActtctt < 5:107607/136‑1 (MQ=255) | GCCCGTGCCACGGCTCTCACTATGACCTGGCTGGTCGTGTCTACAAGTCCCAGCCGGCGCCTCTCAACCTGCCCGTGCCACCGCACTCTTATGAGTCGGACGAGATCATCGTCATTGGCGTCGACCAGGAGAACGCATGATGAGCAAGTTCATGGACTGGATTGATGCTCGCTTCCCCGCCACCAAGATGTGGGAAGACCACCTCAGCAAGTATTACGCACCCAAGAACTTCAACTTCCTGTACTTCTT > ACB122/1497806‑1498054 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |