Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,806,734 C→A 100% S11R (AGC→AGA)  yeiG → S‑formylglutathione hydrolase YeiG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,806,7340CA100.0% 111.1 / NA 35S11R (AGC→AGA) yeiGS‑formylglutathione hydrolase YeiG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (16/19);  total (16/19)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACGATGGGGCTGGAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTG  >  ACB122/1806613‑1806848
                                                                                                                         |                                                                                                                  
acGATGGGGCTGGAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGATTCGGCGGCTGGca                                                                                                      >  2:154469/1‑136 (MQ=255)
        gCTGGAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGATTCGGCGGCTGGc                                                                                                       >  4:93863/1‑127 (MQ=255)
        gCTGGAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGATTCGGCGGCTGGc                                                                                                       <  3:93863/127‑1 (MQ=255)
             aaGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGTATAACATCTCCTGCCAGAAGAGATTCGGCGGCTGGCACAAGCGTTACCgg                                                                                         >  5:26630/1‑136 (MQ=255)
             aaGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGATTCGGCGGCTGGCACAAGCGTTACCgg                                                                                         <  7:168875/136‑1 (MQ=255)
                     aacaaGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGATTCGGCGGCTGGCACAAGCGTTACCGGCATCAtt                                                                                  <  2:73622/135‑1 (MQ=255)
                     aacaaGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGATTCGGCGGCTGGCACAAGCGTTACCGGCATCATTc                                                                                 >  2:74012/1‑136 (MQ=255)
                         aGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGATTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAg                                                                             <  6:159089/136‑1 (MQ=255)
                             cTTCAACCTGATGCATGAAGGCAAGAGCATCCCTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGATTCGGCGGCTCGCACAAGCGATACCGTCATCATTCCAAGGTGc                                                                         >  7:145958/1‑136 (MQ=255)
                               gcGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGATTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTg                                                                       <  7:89499/135‑1 (MQ=255)
                                cGACCTTATGCATGAAGGCAAGAGCATCCGTAGAGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGATTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCt                                                                        <  5:152835/134‑1 (MQ=255)
                                  aCCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGATGAGATTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGt                                                                    >  8:148766/1‑136 (MQ=255)
                                   ccTGATGCATGAAGGCAAGAGCATCCGTAGAGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGATTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGCTGCGGGGtt                                                                   >  1:103446/1‑136 (MQ=255)
                                         gCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGATTCGGCGGCTGGCACAAGCGTTACCGGcatcaat                                                                                  <  5:119041/115‑3 (MQ=255)
                                         gCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGATTCGGCGGCTGGCACAAGCGTTACCGGcatcaat                                                                                  >  6:119041/1‑113 (MQ=255)
                                            tGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGATTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATgg                                                          <  5:160817/136‑1 (MQ=255)
                                              aaGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGATTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGgt                                                         <  1:74012/135‑1 (MQ=255)
                                              aaGGCAAGAGCATCCGTAGCGTGAGCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGATTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGgt                                                         <  4:30572/135‑1 (MQ=255)
                                                 gCAAGAGCATCCGTAGCGTGATCCTCTTCTGAGGTCTGTCATGATCCTGGATAACATCTCCTGCCAGAAGAGATTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTc                                                     <  6:26630/136‑1 (MQ=255)
                                                            cGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGATTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAg                                                                             <  8:1276/101‑1 (MQ=255)
                                                            cGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGATTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAg                                                                             >  7:1276/1‑101 (MQ=255)
                                                                gCGTGATCCACTTCGGCGGTCTGTCCTGAGCCTGGATAACATCTCCTGCCAGAAGAGATTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTgccg                                      <  7:66643/136‑1 (MQ=255)
                                                                       ccACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGATTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAgg                               >  1:116189/1‑136 (MQ=255)
                                                                        cACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGATTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGc                              >  8:2677/1‑136 (MQ=255)
                                                                                        cATGAGCCTGGATAACATCTCCTGCCAGAAGAGATTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTAGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAg              >  5:91883/1‑136 (MQ=255)
                                                                                         aTGAGCCTGGATAACATCTCCTGCCAGAAGAGATGCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGc             <  8:120391/136‑1 (MQ=255)
                                                                                               caggATAACATCTCCTCCCAGAAGAGATTCGGCGGCTTGCACAAGCGTTACAGCCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGCGCGAAAATCTGCCgg       >  8:156563/3‑136 (MQ=255)
                                                                                               cTGGATAACATCTCCTGCCAGAAGAGATTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCgg       <  5:103841/136‑1 (MQ=255)
                                                                                               cTGGATAACATCTCCTGCCAGAAGAGATTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCgg       <  4:174315/136‑1 (MQ=255)
                                                                                               cTGGATAACATCTCCTGCCAGAAGAGATTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCgg       <  2:116189/136‑1 (MQ=255)
                                                                                                   aTAACATCTCCTGCCACAAGAGATTCGGCGGCTGGCACAAGCGGTACCGGCATCATTCCAAGGGGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCt   <  1:21603/136‑1 (MQ=255)
                                                                                                    tAACATCTCCTGCCAGAAGAGATTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTg  >  5:68203/1‑136 (MQ=255)
                                                                                                    tAACATCTCCTGCCAGAAGAGATTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCCGTGCTg  >  3:27487/1‑136 (MQ=255)
                                                                                                                     agagATTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAg                                <  2:108634/89‑1 (MQ=255)
                                                                                                                     agagATTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAg                                >  1:108634/1‑89 (MQ=255)
                                                                                                                         |                                                                                                                  
ACGATGGGGCTGGAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTG  >  ACB122/1806613‑1806848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑