| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 1,920,126 | C→T | 11.1% | intergenic (‑205/‑123) | CJ019_01835 ← / → phnR | hypothetical protein/Putative transcriptional regulator of 2‑aminoethylphosphonate degradation operons |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 1,920,126 | 0 | C | T | 11.1% | 99.6 / 3.5 | 36 | intergenic (‑205/‑123) | CJ019_01835/phnR | hypothetical protein/Putative transcriptional regulator of 2‑aminoethylphosphonate degradation operons |
| Reads supporting (aligned to +/- strand): ref base C (13/19); new base T (2/2); total (15/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTCTAGGTCAGCAAACCTTGAGCCAAGGTAGGCCGCTTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCTGCATAGGCTTGCACTATTCTCAACCTGCCCTGTGATGGGGCTGGACTAGTCCAGATAGGTAACCTTTCG > ACB122/1920000‑1920248 | gTCTAGGTCAGCAAACCTTGAGCCAAGGTAGGCCGCTTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGACCCGGTTCTGCTGGCTTGGCTCGTTTCTg > 8:53707/1‑136 (MQ=255) ggTCAGCAAACCTTGTGCCAAGGTTGGCCGCTTGCGTGACTGTTGGCTGTAGGGAGGGGGCCGGCAAGGGCCCGGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGt < 2:98921/136‑1 (MQ=255) ggTCAGCAAACCTTGAGCCAAGGTAGGCCGCTTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGt < 6:98534/136‑1 (MQ=255) gAGCCAAGGTAGGCCGCTTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCt > 7:88588/1‑136 (MQ=255) aaGGTAGGCCGCTTGCGTGACAGTTTGATGGAGGGCGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTc < 4:127650/136‑1 (MQ=255) ggTTGGCCGCTTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTcac < 7:52377/136‑1 (MQ=255) tAGGCCGCTTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAg > 3:44162/1‑136 (MQ=255) aGGCCGCTTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCCAGCTTTGTCACAgg > 5:125775/1‑136 (MQ=255) ggCCGCTTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAgg < 4:44162/135‑1 (MQ=255) gCTTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGtt < 5:97730/136‑1 (MQ=255) gCTTGCGTGAAAGTTTGATGTAGGGAGGGGGCCGNCANGNGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGtt < 5:100931/136‑1 (MQ=255) ttGCTTGACTGTTTGATTTTGGGAGTGGGCCGGCAAGGGCCCTGAAGCTACATCGTAGACGAGGTAATCGCGCCGGTTCTGCTGGCTTTGCTCGTTTATGTTAGTGACTGGCCAAAGCTTTGTCACAGGATGtt < 8:183298/134‑1 (MQ=255) gCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGTGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTtcat > 6:85652/1‑135 (MQ=255) gCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGTGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTtcat > 6:82260/1‑135 (MQ=255) cGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTtcatc < 5:83910/135‑1 (MQ=255) cGTGACAGTTTGATGTAGGGAGGGCGCCGGCAAGGGCACTGAAGCTACAGCGTAGACCAGGTAATCGCCACGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCGCAAGCTTTGTCTCAGGATGTtcatca < 1:57325/136‑1 (MQ=255) cAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCt < 4:74751/136‑1 (MQ=255) cAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCt < 7:64293/136‑1 (MQ=255) tAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGTGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCTGCATAGGCtt < 5:82260/136‑1 (MQ=255) tAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGTGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCTGCATAGGCtt < 5:85652/136‑1 (MQ=255) tAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCTGCATAGGCtt > 6:101235/1‑136 (MQ=255) tAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCTGCATAGGCtt < 6:179470/136‑1 (MQ=255) tAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCTGCATAGGCtt < 3:48148/136‑1 (MQ=255) gggggCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCTGCATAGGCTTGCACTa > 3:141608/1‑136 (MQ=255) gCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCTGCATAGGCTTGCACTATtct > 3:157092/1‑136 (MQ=255) ccGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCTGCATAGGCtt > 5:110009/1‑125 (MQ=255) ccGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCTGCATAGGCtt < 6:110009/125‑1 (MQ=255) cAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCTGCATAGGCTTGCACTATTCTCAAcc > 4:82774/1‑136 (MQ=255) aaGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCTGCATAGGCTTGCACTATTCTCAACCt > 3:191843/1‑136 (MQ=255) ccTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCTGCATAGGCTTGCACTATTCTCAACCTGCCCtg < 8:88588/136‑1 (MQ=255) cTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCTGCATAGGCTTGCACTATTCTCAACCTGCCCtgt < 5:194531/136‑1 (MQ=255) cAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCTGCATAGGCTTGCACTATTCTCAACCTGCCCTGTGATGGGGCt < 3:82774/136‑1 (MQ=255) aCCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCTGCATAGGCTTGCACTATTCTCAACCTGCCCTGTGATGGGGCTGGACTAGt > 3:197439/1‑136 (MQ=255) aGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGGTCAACAGCCCTGCAGAGTCTTGCACTATTCTCAACCTGCCCTGTGATGGGGCTGGACTAGTc > 8:149284/1‑134 (MQ=255) aTCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCTGCATAGGCTTGCACTATTCTCAACCTGCCCTGTGATGGGGCTGGACTAGTCCAGATAg > 5:87742/1‑136 (MQ=255) ctgctgGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCTGCATAGGCTTGCACTATTCTCAACCTGCCCTGTGATGGGGCTGGACTAGTCCAGATAGGTAACCTTTCg < 4:157092/135‑1 (MQ=255) | GTCTAGGTCAGCAAACCTTGAGCCAAGGTAGGCCGCTTGCGTGACAGTTTGATGTAGGGAGGGGGCCGGCAAGGGCCCTGAAGCTACAGCGTAGACCAGGTAATCGCCCCGGTTCTGCTGGCTTGGCGCGTTTCTGGCAGTGCCTGGCCCAAGCTTTGTCACAGGATGTTCATCAGCCCTGCATAGGCTTGCACTATTCTCAACCTGCCCTGTGATGGGGCTGGACTAGTCCAGATAGGTAACCTTTCG > ACB122/1920000‑1920248 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |