Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 2,006,436 G→A 8.5% P175S (CCT→TCT)  CJ019_01905 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1222,006,4360GA 8.5% 144.7 / 2.8 47P175S (CCT→TCT) CJ019_01905hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (26/17);  new base A (2/2);  total (28/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCTTGGGGTTTGGCACACTTACACGCAAGCCGCCATGAGTGGTGCAAAGGCCGGCAGCGCTGAGTAAGGGGTGGGGTATGCTGGCGACGGCGTCAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGGAGCCTGCAGGTTGCCAAGGTCAATGGCCCATCCCTGGATCTCTCTGTGTGTCTCCAGGTACCGCACCACACCTATCCTACGAGGGGCGTCGGCCTGCGCCGCGAGCAGCTTGATCACTTGGGCTAATTCTT  >  ACB122/2006303‑2006567
                                                                                                                                     |                                                                                                                                   
gcCTTGGGGTTTGGCACACTTACACGCAAGCCGCCATGAGTGGTGCAAAGGCCGGCAGCGCTGAGTAAGGGGTGGGGTATGCTGGCGACGGCGTCAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGGAg                                                                                                                                   >  6:191891/1‑136 (MQ=255)
 ccTTGGGGTTTGGCACACTTACACGCAAGCCGCCATGAGTGGTGCAAAGGCCGGCAGCGCTGAGTAAGGGGTGGGGTATGCTGGCGACGGCGTCAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGGAGc                                                                                                                                  >  8:81936/1‑136 (MQ=255)
     ggggTTTGGCACACTTACACGCAAGCCGCCATGAGTGGTGCAAAGGCCGGCAGCGCTGAGTAAGGGGTGGGGTATGCTGGCGACGGCGTCAACGTTCACGCTGTCAGAATCGATCTTCAAGGACGCAGGAGCCTGc                                                                                                                              <  1:189373/136‑1 (MQ=255)
              cacaCTTACACGCAAGCCGCCATGAGTGGTGCAAAGGCCGGCAGCGCTGAGTAAGGGGTGGGGTATGCTGGCGACGGCGTCAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGGAGCCTGCAGGTTGcc                                                                                                                      >  2:35308/1‑135 (MQ=255)
               acacTTACACGCAAGCCGCCATGAGTGGTGCAAAGGCCGGCAGCGCTGAGTAAGGGGTGGGGTATGCTGGCGACGGCGTCAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGGAGCCTGCAGGTTGcc                                                                                                                      >  2:58362/1‑134 (MQ=255)
                      cacGCAAGCCGCCATGAGTGGTGCAAAGGCCGGCAGCGCTGAGTAAGGGGTGGGGTATGCTGGCGACGGCGTCAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGGAGCCTGCAGGTTGCCAAGGTCAAt                                                                                                             >  5:163863/1‑136 (MQ=255)
                       acGCAAGCCGCCATGAGTGGTGCAAAGGCCGGCAGCGCTGAGTAAGGGGTGGGGTATGCTGGCGACGGCGTCAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGGAGCCTGCAGGTTGCCAAGGTCAATg                                                                                                            <  4:22153/136‑1 (MQ=255)
                       acGCAAGCCGCCATGAGTGGTGCAAAGGCCGGCAGCGCTGAGTAAGGGGTGGGGTATGCTGGCGACGGCGTCAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGGAGCCTGCAGGTTGCCAAGGTCAATg                                                                                                            <  4:19580/136‑1 (MQ=255)
                              ccgccATGAGTGGTGCAAAGGCCGGCAGCGCTGAGTAAGGGGTGGGGTATGCTGGCGACGGCGTCAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGGAGCCTGCAGGTTGCCAAGGTCAATGGCCCATc                                                                                                     >  5:50460/1‑136 (MQ=255)
                                gccATGAGTGGTGCAAAGGCCGGCAGCGCTGAGTAAGGGGTGGGGTATGCTGGCGACGGCGTCCACGTTCACGCTGTCAGCCTCGATCTTCAAGGCCGCAGGAGCCTGCATGTTGCCAAGGTCAATGTCCCATccc                                                                                                   >  4:105745/1‑136 (MQ=255)
                                        tggtgCAAAGGCCGGCAGCGCTGAGTAAGGGGTGGGGTATGCTGGCGACGGCGTCAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGGAGCCTGCAGGTTGCCAAGGTCAATGGCCCATCCCTGGAtctc                                                                                           >  8:107064/1‑136 (MQ=255)
                                          gtgCAAAGGCCGGCAGCGCTGAGTAAGGGGTGGGGTATGCTGGCGACGGCGTCAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGGAGCCTGCAGGTTGCCAAGGTCAATGGCCCATCCCTGGAtctctc                                                                                         <  5:191891/136‑1 (MQ=255)
                                           tgCAAAGGCCGGCAGCGCTGAGTAAGGGGTGGGGTATGCTGGCGACGGCGTCAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGGAGCCTGCAGGTTGCCAAGGTCAATGGCCCATCCCTGGATctctct                                                                                        <  7:107064/136‑1 (MQ=255)
                                            gCAAAGGCCGGCAGCGCTGAGTAAGGGGTGGGGTATGCTGGCGACGGCGTCAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGGAGCCTGCAGGTTGCCAAGGTCAATGGCCCATCCCTGGATCTCTCtg                                                                                       >  1:142429/1‑136 (MQ=255)
                                              aaaGGCCGGCAGCGCTGAGTAAGGGGTGGGGTATGCTGGCGACGGCGTCAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGGCGCCTGCAGGTTGCCAAGGTCAATGGCCCATCCCTGGATCTCTCtgtg                                                                                     >  4:142090/1‑136 (MQ=255)
                                              aaaGGCCGGCAGCGCTGAGTAAGGGGTGGGGTATGCTGGCGACGGCGTCAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGGAGCCt                                                                                                                                >  4:160423/1‑93 (MQ=255)
                                              aaaGGCCGGCAGCGCTGAGTAAGGGGTGGGGTATGCTGGCGACGGCGTCAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGGAGCCt                                                                                                                                <  3:160423/93‑1 (MQ=255)
                                                aGGCCGGCAGCGCTGAGTAAGGTGTGGGGTATGCTGGCGACGGCGTCAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGGAGCCTGCAGGTTGCCAAGGTCAATGGCCCATCCCTGGATCTCTCtgtgtg                                                                                   >  1:117418/1‑136 (MQ=255)
                                                     ggCAGCGCTGAGTAAGGGGTGGGGTATGCTGGCGACGGCGTCAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGGAGCCTGCAGGTTGCCAAGGTCAATGGCCCATCCCTGGATCTCTCTGTGTGtctc                                                                               >  7:119209/1‑135 (MQ=255)
                                                       cAGCGCTGAGTAAGGGGTGGGGTATGCTGGCGACGGCGTCAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGGAGCCTGCAGGTTGCCAAGGTCAATGGCCCATCCCTGGATCTCTCTGTGTGTCTCCAg                                                                            <  7:81936/136‑1 (MQ=255)
                                                        aGCGCTGAGTAAGGGGTGGGGTATGCTGGCGACGGCGTCAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGGAGCCTGCAGGTTGCCAAGGTCAATGGCCCATCCCTGGATCTCTCTGTGTGTCTCCAgg                                                                           >  7:156176/1‑136 (MQ=255)
                                                        aGCGCTGAGTAAGGGGTGGGGTATGCTGGCGACGGCGTCAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGGAGCCTGCAGGTTGCCAAGGTCAATGGCCCATCACTGGATCTCTCTGTGTGTCTCCAgg                                                                           >  3:177135/1‑136 (MQ=255)
                                                               aGTAAGGGGTGGGGTATGCTGGCGACGGCGTCAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGGAGCCTGCAGGTTGCCAAGGTCAATGGCCCATCCCTGGATCTCTCTGTGTGTCTCCAGGTACCg                                                                      <  6:163863/134‑1 (MQ=255)
                                                               aGTAAGGGGTGGGGTATGCTGGCGACGGCGTCAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGGAGCCTGCAGGTTGCCAAGGTCAATGGCCCATCCCTGGATCTCTCTGTGTGTCTCCAGGTACCGca                                                                    >  1:5604/1‑136 (MQ=255)
                                                               aGTAAGGGGGGGTGTATGCTGGCGACGGAGTCAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGGAGCCTGCAGGTTGCCAAGGTCAATGGCCCATCCCTGGATCTCTCTGTGTGTCTCCAGGTACCg                                                                      <  3:105745/134‑1 (MQ=255)
                                                                  aaGGGGTGGGGTATGCTGGCGACGGCGTCAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGGAGCCTGCAGGTTGCCAAGGTCAATGGCCCATCCCTGGATCTCTCTGTGTGTCTCCAGGTACCGcacca                                                                 >  5:177965/1‑136 (MQ=255)
                                                                   aGGGGTGGGGTATGCTGGCGACGGCGTCAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGGAGCCTGCAGGTTGCCAAGGTCAATGGCCCATCCCTGGATCTCTCTGTGTGTCTCCAGGTACCGcacca                                                                 <  8:156176/135‑1 (MQ=255)
                                                                     gggtggggtATGCTGGCGACGGCGTCAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGGAGCCTGCAGGTTGCCAAGGTCAATGGCCCATCCCTGGATCTCTCTGTGTGTCTCCAGGTACCGCACCacac                                                              <  6:50460/136‑1 (MQ=255)
                                                                       gtggggtATGCTGGCGACGGCGTCAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGGAGCCTGCAGGTTGCCAAGGTCAATGGCCCATCCCTGGATCTCTCTGTGTGTCTCCAGGTACCGCACCACAcc                                                             >  1:44060/1‑135 (MQ=255)
                                                                            gtATGCTGGCGACGGCGTCAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGGAGCCTGCAGGTTGCCAAGGTCAATGGCCCATCCCTGGATCTCTCTGTGTGTCTCCAGGTACCGCCCCACACCTATcc                                                        >  8:129960/1‑135 (MQ=255)
                                                                            gtATGCTGGCGACGGCGTCAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGGAGCCTGCAGGTTGCCAAGGTCAATGGCCCATCCCTGGATCTCTCTGTGTGTCTCCAGGTACCGCACCACACCTATcc                                                        >  7:21027/1‑135 (MQ=255)
                                                                            gtATGCTGGCGACGGCGTCAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGAAGCCTGCAGGTTGCCAAGGTCAATgg                                                                                                           <  8:197428/84‑1 (MQ=255)
                                                                            gtATGCTGGCGACGGCGTCAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGAAGCCTGCAGGTTGCCAAGGTCAATgg                                                                                                           <  8:194319/84‑1 (MQ=255)
                                                                            gtATGCTGGCGACGGCGTCAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGAAGCCTGCAGGTTGCCAAGGTCAATgg                                                                                                           >  7:194319/1‑84 (MQ=255)
                                                                            gtATGCTGGCGACGGCGTCAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGAAGCCTGCAGGTTGCCAAGGTCAATgg                                                                                                           >  7:197428/1‑84 (MQ=255)
                                                                              aTGCTGGCGACGGCGTCAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGGAGCCTGCAGGTTGCCAAGGTCAATGGCCCATCCCTGGATCTCTCTGTGTGTCTCCAGGTACCGCACCACACCTATCCTAc                                                     >  8:99392/1‑136 (MQ=255)
                                                                                  tGGCGACGGCGTAACCGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGGAGCCTGCAGGTTGCCAAGGTCAATGGCCCATCCCTGGATCTCTCTGTGTGTCTCCAGGTACCGCACCACACCTAACCTACGAgg                                                 >  8:145120/1‑136 (MQ=255)
                                                                                   ggCGACGGCGTCAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGGAGCCTGCAGGTTGCCAAGGTCAATGGCCCCTCCCTGGCTCTCTCTGTGTGTCTCCAGGTCCCGCACCACCCCGATCCTCCGAggg                                                >  8:49514/1‑136 (MQ=255)
                                                                                    gCGACGGCGTCAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGGAGCCTGCAGGTTGCCAAGGTCAATGGCCCATCCCTGGATCTCTCTGTGTGTCTCCAGGTACCGCACCACACCTATCCTACGAggg                                                <  7:23546/135‑1 (MQ=255)
                                                                                    gCGACGGCGTCAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGGAGCCTGCAGGTTGCCAAGGTCAATGGCCCATCCCTGGATCTCGCTGTGTGTCTCCAGGTACCGCACCACACCTATCCTACGAggg                                                <  2:44060/135‑1 (MQ=255)
                                                                                          gCGTCAACGTTCACGCTGTCAGCCTCGATCATCAAGGACGCAGGAGCCTGCAGGTTGCCAAGGTCAATGGCCCATCCCTGGATCTCTCTGTGTGTCTCCAGGTACCGCACCACACCTATCCTACGAGGGGCGTCgg                                         <  1:72671/136‑1 (MQ=255)
                                                                                            gTCAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGGAGCCTGCAGGTTGCCAAGGTCAATGGCCCATCCCTGGATCTCTCTGTGTGTCTCCAGGTACCGCACCACACCTATCCTACGAGGGGCGTCGGcc                                       >  4:171109/1‑136 (MQ=255)
                                                                                              cAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGGAGCCTGCAGGTTGCCAAGGTCAATGGCCCATCCCTGGATCTCTCTGTGTGTCTCCAGGTACCg                                                                      <  6:79517/103‑1 (MQ=255)
                                                                                              cAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGGAGCCTGCAGGTTGCCAAGGTCAATGGCCCATCCCTGGATCTCTCTGTGTGTCTCCAGGTACCg                                                                      >  5:79517/1‑103 (MQ=255)
                                                                                                            tCAGCCTCGATCTTCAAGGACGCAGGAGCCTGCAGGTTGCCAAGGTCAATGGCCCATCCCTGGATCTCTCTGTGTGTCTCCAGGTACCGCACCACACCTATCCTACGAGGGGCGTCGGCCTGCGCCGCGAGCAGCt                       <  2:142429/136‑1 (MQ=255)
                                                                                                               gCCTCGATCTTCAAGGACGCAGGAGCCTGCAGGTTGCCAAGGTCAATGGCCCATCCCTGGATCTCTCTGTGTGTCTCCAGTTACCGCACCACACCTATCCTACGACGGGCGTCGGCCTGCGCCGCGAGCAGCTTGa                    >  4:180345/1‑136 (MQ=255)
                                                                                                                                 gCAGGAGCCTGCAGGTTGCCAAGGTCAATGGCCCATCCCTGGATCTCTCTGTGTGTCTCCAGGTACCGCACCACACCTATCCTACGAGGGGCGTCGGCCTGCGCCGCGAGCAGCTTGATCACTTGGGCTAATTCtt  <  4:75392/136‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                   
GCCTTGGGGTTTGGCACACTTACACGCAAGCCGCCATGAGTGGTGCAAAGGCCGGCAGCGCTGAGTAAGGGGTGGGGTATGCTGGCGACGGCGTCAACGTTCACGCTGTCAGCCTCGATCTTCAAGGACGCAGGAGCCTGCAGGTTGCCAAGGTCAATGGCCCATCCCTGGATCTCTCTGTGTGTCTCCAGGTACCGCACCACACCTATCCTACGAGGGGCGTCGGCCTGCGCCGCGAGCAGCTTGATCACTTGGGCTAATTCTT  >  ACB122/2006303‑2006567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑