| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 2,384,716 | G→T | 11.9% | S63I (AGC→ATC) | CJ019_02252 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 2,384,716 | 0 | G | T | 11.9% | 94.7 / 5.6 | 40 | S63I (AGC→ATC) | CJ019_02252 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (15/20); new base T (2/3); total (17/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.21e-01 | |||||||||||
CGTCCGCCTGGGCGATGGCGCTGATTGCACTGGTGGCGGGCGGCTTGTTGACCGCTGCCTTGGCATTTGCCGCGCACACCTTCTACAAACAGCAGCTGCGCCAGCGCTTCGAGCTGCTGGCCAGCGAGCGCGCCAGCCGTATTGCCGAGCGCTTTGACGAACAGCAGCAGCGCTTGGACGGGCTGCGGCGGTTCTTCAGTTTTTCCAGCGAGATCACCCCGCGCGAATTCGACGGCTATGCCCGACCACTGCTGCAACGCACCTT > ACB122/2384581‑2384845 | cGTCCGCCTGGGCGATGGCGCTGATTGCACTGGTGGCGGGCGGCTTGTTGACCGCTGCCTTGGCATTTGCCGCGCACACCTTCTACAAACAGCAGCTGCGCCAGCGCTTCGAGCTGCTGGCCAGCGAGCGCGCCAg > 4:174456/1‑136 (MQ=255) gTCCGCCTGGGCGATGGCGCTGATTGCACTGGTGGCGGGCGGCTTGTTGACCGCTGCCTTGGCATTTGCCGCGCACACCTTCTACAAACAGCAGCTGCGCCAGCGCTTCGAGCTGCTGGCCAGCGAGCGCGCCAGc > 3:140796/1‑136 (MQ=255) cTGGGCGATGGCGCTGATTGCACTGGTGGCGGGCGGCTTGTTGACCGCTGCCTTGGCATTTGCCGCGCACACCTTCTACAAACAGCAGCTGCGCCAGCGCTTCGAGCTGCTGGCCAGCGAGCGCGCCAGCCGTAtt > 2:117292/1‑136 (MQ=255) cTGGGCGATGGCGCTGATTGCACTGGTGGCGGGCGGCTTGTTGACCGCGGCCTTGGCATTTGCCGCGCACACCTTCTACAAACAGCAGCTGCGCCAGCGCTTCGAGCTGCTGGCCAGCGAGCGCGCCAGCCGTAtt < 2:4923/136‑1 (MQ=255) cGATGGCGCTGATTGCACTGGTGGCGGGCGGCTTGTTGACCGCTGCCTTGGCATTTGCCGCGCACACCTTCTACAAACAGCAGCTGCGCCNNCNCTTCGAGCTNNTGGCCAGCGAGCGCGCCAGCCGTATTGcc > 5:178253/1‑134 (MQ=255) cgcTGATTGCACTGGTGGCGGGCGGCTTGTTGACCGCTGCCTTGGCATTTGCCGCGCCCCCCTTCTACAAACAGCAGCTGCGCCAGCGCTTGGAGCTGCTGGCCAGCGAGCGCGCCAGCCGTATTGCCGAGCGCtt > 7:85677/1‑136 (MQ=255) cTGATTGCACTGGTGGCGGGCGGCTTGTTGACCGCTGCCTTGGCATTTGCCGCGCACACCTTCTACAAACAGCAGCTGCGCCAGCGCTTCGAGCTGCTGGCCAGCGAGCGCGCCAGCCGTATTGCCGAGCGCTTTg < 8:34477/136‑1 (MQ=255) cTGATTGCACTGGTGGCGGGCGGCTTGTTGACCGCTGCCTTGGCATTTGCCGCGCACACCTTCTACAAACAGCAGCTGCGCCAGCGCTTCGAGCTGCTGGCCAGCGAGCGCGCCAGCCGTATTGCCGAGCGCTTTg < 3:166874/136‑1 (MQ=255) ggcTTGTTGACCGCTGCCTTGGCATTTGCCGCGCACACCTTCTACAAACAGCAGCTGCGCCAGCGCTTCGAGCTGCTGGCCAGCGAGCGCGCCAGCCGTATTGCCGAGCGCTTTGACGAACAGCAGCAGCGCTTgg > 7:165409/1‑136 (MQ=255) tgttgACCGCTGCCTTGGCATTTGCCGCGCACACCTTCTACAAACAGCAGCTGCGCCAGCGCTTCGAGCTGCTGGCCAGCGAGCGCGCCATCCGTATTGc < 8:15007/100‑1 (MQ=255) tgttgACCGCTGCCTTGGCATTTGCCGCGCACACCTTCTACAAACAGCAGCTGCGCCAGCGCTTCGAGCTGCTGGCCAGCGAGCGCGCCATCCGTATTGc > 1:78575/1‑100 (MQ=255) tgttgACCGCTGCCTTGGCATTTGCCGCGCACACCTTCTACAAACAGCAGCTGCGCCAGCGCTTCGAGCTGCTGGCCAGCGAGCGCGCCATCCGTATTGc > 7:15007/1‑100 (MQ=255) tgttgACCGCTGCCTTGGCATTTGCCGCGCACACCTTCTACAAACAGCAGCTGCGCCAGCGCTTCGAGCTGCTGGCCAGCGAGCGCGCCATCCGTATTGc < 2:78575/100‑1 (MQ=255) tgttgACCGCTGCCTTGGCATTTGCCGCGCACACCTTCTACAAACAGCAGCTGCGCCAGCGCTTCGAGCTGCTGGCCAGCGAGCGCGCCAGCCGTATTGCCGAGCGCTTTGACGAACAGCAGCAGCGCTTGGACgg > 6:199017/1‑136 (MQ=255) cccgccgCCTTGGCATTTGCCGCGCACTACTTCTACAAACCGCAGCTGCGCCAGCGCTGCGAGCTGCGGGCCAGCGAGCGCGCCAGCCGTAGTGCCGAGCGCTTTGACGAACAGCAGCAGCGCTTGGACGGGCTgc < 3:130126/135‑1 (MQ=255) cGCTGCCTTGGCATTTGCCGCGCACACCTTCTACAAACAGCAGCTGCGCCAGCGCTTCGAGCTGCTGGCCAGCGAGCGCGCCAGCCGTATTGCCGAGCGCTTTGACGAACAGCAGCAGCGCTTGGACGGGCTgcgg > 1:22842/1‑136 (MQ=255) aTTTGCCGCGCACACCTTCTACAAACAGCAGCTGCGCCAGCGCTTCGAGCTGCTGGCCAGCGAGCGCGCCAGCCGTATTGCCGAGCGCTTTGACGAACAGCAGCAGCGCTTGGACGGGCTGCGGCGGTTCTTCAGt < 8:165409/136‑1 (MQ=255) ccGCGCACACCTTCTACAAACAGCAGCTGCGCCAGCGCTGCGAGCTGCTGGCCAGCGAGCGCGCCAGCCGTATTGCCGAGCGCTTTGACGAACAGCAGCAGCGCTTGGACGGGCTGCGGCGGTTCTTCAGTTTTTc < 5:111801/136‑1 (MQ=255) cgcgCACACCTTCTACAAACAGCAGCTGCGCCAGCGCTTCGAGCTGCTGGCCAGCGAGCGCGCCAGCCGTATTGCCGAGCGCTTTGACGAAcagca < 1:23461/96‑1 (MQ=255) cgcgCACACCTTCTACAAACAGCAGCTGCGCCAGCGCTTCGAGCTGCTGGCCAGCGAGCGCGCCAGCCGTATTGCCGAGCGCTTTGACGAAcagca > 2:23461/1‑96 (MQ=255) acacCTTCTACAAACAGCAGCTGCGCCAGCGCTTCGAGCTGCTGGCCAGCGAGCGCGCCAGCCGTATTGCCGAGCGCtt > 3:49906/1‑79 (MQ=255) acacCTTCTACAAACAGCAGCTGCGCCAGCGCTTCGAGCTGCTGGCCAGCGAGCGCGCCAGCCGTATTGCCGAGCGCtt < 4:49906/79‑1 (MQ=255) aaaCAGCAGCTGCGCCAGCGCTTCGAGCTGCTGGCCAGCGAGCGCGCCAGCCGTATTGCCGAGCGCTTTGACGAACAGCAGCAGCGCTTGGACGGGCTGCGGCGGTTCTTCAGTTTTTCCAGCGAGATCACCCcgc < 6:161574/136‑1 (MQ=255) cagcTGCGCCAGCGCTTCGAGCTGCTGGCCAGCGAGCGCGCCAGCCGTATTGCCGAGCGCTTTGACGAACAGCAGCAGCGCTTGGACGGGCTGCGGCGGTTCTTCAGTTTTTCCAGCGAGATCACCCCGCGCGAAt > 8:77823/1‑136 (MQ=255) cagcTGCGCCAGCGCTTCGAGCTGCTGGCCAGCGAGCGCGCCAGCCGTATTGCCGAGCGCTTTGACGAACAGCAGCAGCGCTTGGACGGGCTGCGGCGGTTCTTCAGTTTTTCCAGCGAGATCACCCCGCGCGAAt > 2:178390/1‑136 (MQ=255) cagcTGCGCCAGCGCTTCGAGCTGCTGGCCAGCGAGCGCGCCAGCCGTATTGCCGAGCGCTTTGACGAACAGCAGCAGCGCTTGGACGGGCTGCGGCGGTTCTTCAGTTTTTCCAGCGAGATCACCCCGCGCGAAt < 1:117292/136‑1 (MQ=255) cTGCGCCAGCGCTTCGAGCTGCTGGCCAGCGAGCGCGCCAGCCGTATTGCCGAGCGCTTTGACGAACAGCAGCAGCGCTTGGACGGGCTGCGGCGGTTCTTCAGTTTTTCCAGCGAGATCACCCCGCGCGAATTCg > 3:123531/1‑136 (MQ=255) cTGCGCCAGCGCTTCGAGCTGCTGGCCAGCGAGCGCGACAGCCGTATTGCCGAGCGCTTTGACGAACAGCAGCAGCGCTTGGACGGGCTGCGGCGGTTCTTCAGTTTTTCCAGCGAGATCACCCCGCGCGAATTc > 6:102607/1‑135 (MQ=255) aGCGCTTCGAGCTGCTGGCCAGCGAGCGCGCCAGCCGTATTGCCGAGCGCTTTGACGAACAGCAGCAGCGCTTGGACGGGCTGCGGCGGTTCTTCAGTTTTTCCAGCGAGATCACCCCGCGCGAATTCGACGGCTa < 2:65205/136‑1 (MQ=255) gcgcTTCGAGCTGCTGGCCAGCGAGCGCGCCAGCCGTATTGCCGAGCGCTTTGACGAACAGCAGCAGCGCTTGGACGGGCTGCGGCGGTTCTTCAGTTTTTCCAGCGAGATCACCCCGCGCGAATTCGACGGCTAt > 8:118284/1‑136 (MQ=255) gtCGCGCTGCTGGCCAGCGAGCTCGCCATCCGTAATGCCGAGCGCTTTGACGAACAGCAGCAGCGATTGGACGGGCTGCGGCGGTTCTTCAGTTTTTCCAGCGAGATCACCCCGCGCGAATTCGACGGCTATGacc < 2:14997/135‑1 (MQ=255) cGAGCTGCTGGCCAGCGAGCGCGCCAGCCGTATTGCCGAGCGCTTTGACGAACAGCAGCAGCGCTTGGACGGGCTGCGGCGGTTCTTCAGTTTTTCCAGCGAGATCACCCCGCGCGAATTCGACGGCTATGccc < 1:65446/134‑1 (MQ=255) cGAGCTGCTGGCCAGCGAGCGCGACAGCCGTATTGCCGAGCGCTTTGACGAACAGCAGCAGCGCTTGGACGGGCTGCGGCGGTTCTTCAGTTTTTCCAGCGAGATCACCCCGCGCGAATTCGACGGCTATGccc < 5:102607/134‑1 (MQ=255) gAGCTGCTGGCCAGCGAGCGCGCCAGCCGTATTGCCGAGCGCTTTGACGAACAGCAGCAGCGCTTGGACGGGCTGCGGCGGTTCTTCAGTTTTTCCAGCGAGATCACCCCGCGCGAATTCGACGGCTATGCCCGAc < 8:36236/136‑1 (MQ=255) gctgctGGCCAGCGAGCGCGCCAGCCGTATTGCCGAGCGCTTTGACGAACAGCAGCAGCGCTTGGACGGGCTGCGGCGGTTCTTCAGTTTTTc > 6:105034/1‑93 (MQ=255) gctgctGGCCAGCGAGCGCGCCAGCCGTATTGCCGAGCGCTTTGACGAACAGCAGCAGCGCTTGGACGGGCTGCGGCGGTTCTTCAGTTTTTc < 5:105034/93‑1 (MQ=255) gCCAGCGAGCGCGCCAGCCGTATTGCCGAGCGCTTTGACGACCAGCAGCAGCGCTTGGACGGGCTGCGGCGGTTCTTCAGTTTTTCCAGCGAGATCACCCCGCGCGAATTCGACGGCTATGCCCGACCActgct < 7:176657/134‑1 (MQ=255) ccAGCGAGCGCGCCAGCCGTATTGCCGAGCGCTTTGACGAACAGCAGCAGCGCTTGGACGGGCTGCGGCGGTTCTTCAGTTTTTCCAGCGAGATCACCCCGCGCGAATTCGACGGCTATGCCCGACCACTGCTGCa < 5:199017/136‑1 (MQ=255) gagcgCGCTAGCCGGATTGCCGAGCGCTTTGACGAACAGCAGCAGCGCTTGGACGGGCTGCGGCGGATCTTCAGTTTTTCCTGCGAGATCACCCCGCGCGAATTGGACGGCTATGCCCGACGACTGCTGCAACGCa < 2:199786/136‑1 (MQ=255) gagcgCGCCAGCCGTATTGCCGAGCGCTTTGACGAACAGCAGCAGCGCTTGGACGGGCTGCGGCGGTTCTTCAGTTTTTCCAGCCTGATCACCCCGCGCGAATTCGACGGCTATGCCCGACCACTGCTGCAACTCa < 4:140796/136‑1 (MQ=255) gcggCAGCCGTATTGGCGAGCTCGTTTACGCACATCAGCATGGCTTGGACGGGGTGCGGCGGTTGTTCATTTTTTGCAGCGAGACCACCCCGCGCGAATTCGACGGCTATGCCCGACCACTGCTGCAACGCACCtt < 3:174456/136‑1 (MQ=255) | CGTCCGCCTGGGCGATGGCGCTGATTGCACTGGTGGCGGGCGGCTTGTTGACCGCTGCCTTGGCATTTGCCGCGCACACCTTCTACAAACAGCAGCTGCGCCAGCGCTTCGAGCTGCTGGCCAGCGAGCGCGCCAGCCGTATTGCCGAGCGCTTTGACGAACAGCAGCAGCGCTTGGACGGGCTGCGGCGGTTCTTCAGTTTTTCCAGCGAGATCACCCCGCGCGAATTCGACGGCTATGCCCGACCACTGCTGCAACGCACCTT > ACB122/2384581‑2384845 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |