Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 2,385,345 T→G 35.6% L273V (TTG→GTG)  CJ019_02252 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1222,385,3450TG35.6% 17.2 / 4.1 24L273V (TTG→GTG) CJ019_02252hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (9/6);  new base G (6/3);  total (15/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGTGGGCTATGTGATGGCGTTGCTGAGCATGCGCGAGCTGGTCAGCGACGGTCGACCGGTAGCGGTTGATGACAACCTGGTCGTGCGCATCACGGACCCGTCCGGGTTACAAGGCCCCGTCGTGTTGTTCGACTCGCAAAATCCGGTCGCGCCCTTGG‑CCCTGGCCAGCAATCAGTTGTTGCGCCTGGCCGATCACCATTTCCAGCTGAGCATTCAGCCAAGCCAGGCGTTCGCGCAGG  >  ACB122/2385220‑2385459
                                                                                                                             |                                                                                                                   
cAGTGGGCTATGTGATGGCGTTGCGGAGCATGCGCGAGCTGGTCAGCGACGGTCGACCGGTAGCGGTTGATGACAACCTGGGCGTGCGCATCACGGACCCGTCCGGGTTACAAGGCCCCGTCGTGTTGTTCGACTc                                                                                                           <  8:158358/136‑1 (MQ=255)
        tATGTGATGGCGTTGCTGAGCATGCGCGAGCTGGTCAGCGACGGTCGACCGGTAGCGGTTGATGACAACCTGGTCGTGCGCATCACGGACCCGTCCGGGTTACAAGGCCCCGTCGTGTTGTTCGACTCGCAAAATc                                                                                                   >  8:29652/1‑136 (MQ=255)
        tATGTGATGGCGTTGCTGAGCATGCGCGAGCTGGTCAGCGACGGTCGACCGGTAGCGGTTGATGACAACCTGGTCGTGCGCATCACGGACCCGTCCGGGTTACAAGGCCCCGTCGGGGTGGTGGGCTCGCAAAAta                                                                                                   >  3:43322/1‑135 (MQ=255)
            tgATGGCGTTGCTGAGCATGCGCGAGCTGGTCAGCGACGGTCGACCGGTAGCGGTTGATGACAACCTGGTCGTGCGCATCACGGACCCGTCCGGGTTACAAGGCCCCGTCGTGTTGTTCGACTCGCAAAATCCgg                                                                                                <  1:35685/135‑1 (MQ=255)
            tgATGGCGTTGCTGAGCAGGCGCGAGCTGGTCAGCGACGTTCGACCGGTAGAGGTTGATGACAACCTGGTCGTGCGCATCACGGACCCGTCCGGGTTACAAGGCCCCGTCGTGTTGTTCGACTCGCAAAATCCgg                                                                                                <  4:196157/135‑1 (MQ=255)
             gATGGCGTTGCTGAGCATGCGCGAGCTGGTCAGCGACGGTCGACCGGTAGCGGTTGATGACAACCTGGTCGTGCGCATCACGGACCCGTCCGGGTTACAAGGCCCCGTCGTGTTGTTGGGCTCGGAAAATCCGGTc                                                                                              >  3:82508/1‑136 (MQ=255)
             gATGGCGTTGCTGAGCATGCGCGAGCTGGTCAGCGACGGTCGACCGGTAGCGGTTGATGACAACCTGGTCGTGCGCATCACGGACCCGTCCGGGTTACAAGGCCCCGGCGTGGTGTTGGGCTCGCAAAAAACGGTc                                                                                              >  5:69446/1‑136 (MQ=255)
                  cGTTGCTGAGCATGCGCGAGCTGGTCAGCGACGGTCGACCGGTAGCGGTTGATGACAACCTGGTCGTGCGCATCACGGACCCGTCCGGGTTACCAGGCCCCGGCGTGGTGTGCCCCTGGCAAAAACCCGTCGCGcc                                                                                         >  7:55155/1‑136 (MQ=255)
                  cGTTGCTGAGCATGCGCGAGCTGGTCAGCGACGGTCGACCGGTAGCGGTTGATGACAACCTGGTCGTGCGCATCACGGACCCGTCCGGGTTACAAGGCCCCGTCGTGTTGTTCGCCGCGAAAAAGACCGTCGCGcc                                                                                         >  8:146971/1‑136 (MQ=255)
                  cGTTGCTGAGCATGCGCGAGCTGGTCAGCGACGGTCGACCGGTAGCGGTTGATGACAACCTGGTCGTGCGCATCACGGACCCGTCCGGGTTACAAGGCCCCGTCGTGGTGTTGGGCGCGCAAAATACCGTCGCGcc                                                                                         >  3:194395/1‑136 (MQ=255)
                   gTTGCTGAGCATGCGCGAGCTGGTCAGCGACGGTCGACCGGTAGCGGTTGATGACAACCTGGTCGTGCGCATCACGGACCCGTCCGGGTTACAAGGCCCCGTCGTGTTGTTCGACTCGCAAAATCCGGTCGCGccc                                                                                        >  7:116855/1‑136 (MQ=255)
                   gTTGCTGAGCATGCGCGAGCTGGTCAGCGACGCTCGACCGGTAGCGGTTGATGACAACCTGGTCGAGCGCCTCACGGACCCGTCCGGGGTACAAGGCCCCGGCGTGTTGATCGCCGCGCCAAATCCGGTCGCGccc                                                                                        >  6:61196/1‑136 (MQ=255)
                    ttGCTGAGCATGCGCGAGCTGGTCAGCGACGGTCGACCGGTAGCGGTTGATGACAACCTGGTCGTGCGCCTCACGGACCCGTCCGGGGTACCAGGCCCCGGCGGGGTGTGTGGCGAGCCAAATCCGGGCGCccccc                                                                                       >  5:73515/1‑135 (MQ=255)
                       cTGAGCATGCGCGAGCTGGTCAGCGACGGTCGACCGGTAGCGGTTGATGACAACCTGGTCGTGCGCATCACGGACCCGTCCGGGTTACAAGGCCCCGTCGTGTTGTTCGCCTCGCAAAATCCGGTCGCGCCCTTgg                                                                                    >  4:66121/1‑136 (MQ=255)
                                  cgAGCTGGTCAGCGACGGTCGACCGGTAGCGGTTGATGACAACCTGGTCGTGCGCATCACGGACCCGTCCGGGGTACAAGGCCCCGGCGTGGTGTTCGACACGCAAAAACCGGGAGCGCCCCTGG‑GCCCGGCCAGc                                                                        >  2:1674/1‑136 (MQ=255)
                                   gcgggggTCCGCGCCGGGCGACCGGTAGCGGTTGATGACAACCTGGTCGTGCGCATCACGGACCCGTCCGGGTTACAAGGCCCCGTCGTGGTGTTCGACTCGCAAAATCCGGTCGCGCCCTTGG‑CCCTGGCCAGCa                                                                       <  5:120096/131‑1 (MQ=255)
                                   gAGCTGGTCAGCGACGGTCGACCGGTAGCGGTTGATGACAACCTGGTCGTGCGCATCACGGACCCGTCCGGGTTACAAGGCCCCGTCGTGTTGTTCGACTCGCAAAATCCGGTCGCGCCCTTGG‑CCCTGGCCAGCa                                                                       <  8:116855/136‑1 (MQ=255)
                                       tGGTCAGCGACGGTCGACCGGTAGCGGTTGATGACAACCTGGTCGTGCGCATCACGGACCCGTCCGGGTTACAAGGCCCCGTCGTGTTGTTCGCCTCGCAAAATCCGGTCGCGCCCTTTG‑CCCGGGCCCGCAAt                                                                     >  8:8610/1‑134 (MQ=255)
                                        ggTCAGCGACGGTCGACCGGTAGCGGTTGATGACAACCTGGTCGTGCGCATCACGGACCCGTCCGGGTTACAAGGCCCCGTCGTGTTGTTCGACTCGCAAAATCCGGTCGCGCCCTTGG‑CCCTGGCCAGCAATCAg                                                                  <  6:46425/136‑1 (MQ=255)
                                                           gTAGCGGTTGATGACAACCTGGTCGTGCGCATCACGGACCCGGCCGGGTTACAAGGCCCCGTCGTGGTGTTCGACTCGCAAAATCCGGTCGCGCCCTTGG‑ACCTGGCCAGCAATCAGTTGTTGCGCCTGGCCGATc                                               <  8:119471/136‑1 (MQ=255)
                                                                 gTTGATGACAACCTGGTCGTGCGCATCACGGACCCGTCCGGGTTACAAGGCCCCGTCGTGTTGTTGGACTCGCCAAATCCGGTCGCGGCCGTGGCCCCGGGCCGGCAA‑CAGTTGTTGCGCCGGGCCGCTGACCaat                                         >  4:90799/1‑134 (MQ=255)
                                                                                                 cccGTCCGGGTTACAAGGCCCCGTCGTGTTGTTCGACTCGCAAAATCCGGTCGCGCCCTTGG‑CCCTGGCCAGCAATCAGTTGTTGCGCCTGGCCGATCACCATTTCCAGCTGAGCATTCAGCCAAGCCAGGCGTTc         <  8:72612/136‑1 (MQ=255)
                                                                                                 cccGTCCGGGTTACAAGGCCCCGTCGTGGTGTTCGACTCGCAAAATCCGGTCGCGCCCTTGG‑CCCTGGCCAGCAATCAGTTGTTGCGCCTGGCCGATCACCATTTCCAGCTGAGCATTCAGCCAAGCCAGGCGTTc         <  6:108440/136‑1 (MQ=255)
                                                                                                        gggTTACAAGGCCCCGTCGTGTTGTTCGACTCGCCAAAACCGGGCGCGGCCGTTG‑CCCGGGGCCGGCCTCAGTTGTTTGGGCAGGGCGCGCACCCATTGAAGCGGAGGATTCAGCCAACCCCGGCGTTAgcgcccg  >  8:76781/1‑133 (MQ=255)
                                                                                                                             |                                                                                                                   
CAGTGGGCTATGTGATGGCGTTGCTGAGCATGCGCGAGCTGGTCAGCGACGGTCGACCGGTAGCGGTTGATGACAACCTGGTCGTGCGCATCACGGACCCGTCCGGGTTACAAGGCCCCGTCGTGTTGTTCGACTCGCAAAATCCGGTCGCGCCCTTGG‑CCCTGGCCAGCAATCAGTTGTTGCGCCTGGCCGATCACCATTTCCAGCTGAGCATTCAGCCAAGCCAGGCGTTCGCGCAGG  >  ACB122/2385220‑2385459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑