Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,033,280 G→T 9.9% V436V (GTG→GTT)  lgoT_1 → putative L‑galactonate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,033,2800GT 9.9% 93.5 / 2.2 38V436V (GTG→GTT) lgoT_1putative L‑galactonate transporter
Reads supporting (aligned to +/- strand):  ref base G (12/22);  new base T (2/2);  total (14/24)
Fisher's exact test for biased strand distribution p-value = 6.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

CAGAACTTCGGCGGCTACCTGGCAGGCTCTGTGGCGCCCATCGTGACCGGGTTCAGCATTCAGCAGACCGGGTCCTTCGCTTCGGCATTCCTGATCAGTGCCGCCGTGGCCGCGGTTGCTGCGATGGCTTATGTGTTGCTGTTGAAGCAGCCGGTTTCCACTGATCGCTGAGCATGCACCTGCAGGCGGATTTCATCAGGCAGTTAAATAACAATTTGTGATGATTTGCATCATGACAATATATTTATCCTTATCAATGTCAGGCCCTAG  >  ACB122/3033146‑3033415
                                                                                                                                      |                                                                                                                                       
cAGAACTTCGGCGGCTACCTGGCAGGCTCTGTGGCGCCCATCGTGACCGGGTTCAGCATTCAGCAGACCGGGTCCTTCGCTTCTGAATTCCTGATCAGTGCCTCCGTGGCCGCGGTTGCTGCGATGGCTTATgtgt                                                                                                                                        >  7:98727/1‑136 (MQ=255)
cAGAACTTCGGCGGCTACCTGGCAGGCTCTGTGGCGCCCATCGTGACCGGGTTCAGCATTCAGCAGACCGGGTCCTTCGCTTCGGCATTCCTGATCAGTGCCGCCGTGGCCGCGGTTGCTGCGATGGCTTATgtgt                                                                                                                                        >  8:65240/1‑136 (MQ=255)
cAGAACTTCGGCGGCTACCTGGCAGGCTCTGTGGCGCCCATCGTGACCGGGTTCAGCATTCAGCAGACCGGGTCCTTCGCTTCGGCATTCCTGATCAGTGCCGCCGTGGCCGCGGTTGCTGCGATGGCTTATgtgt                                                                                                                                        >  8:29985/1‑136 (MQ=255)
           cggcTACCTGGCAGGCTCTGTGGCGCCCATCGTGACCGGGTTCAGCATTCAGCAGACCGGGTCCTTCGCTTCGGCATTCCTGATCAGTGCCGCCGTGGCCGCGGTTGCTGCGATGGCTTATGTGTTGCTGTTGAag                                                                                                                             >  3:89891/1‑136 (MQ=255)
           cggcTACCTGGCAGGCTCTGTGGCGCCCATCGTGACCGGGTTCAGCATTCAGCAGACCGGGTCCTTCGCTTCGGCATTCCTGATCAGTGCCGCCGTGGCCGCGGTTGCTGCGATGGCTTATGTGTTGCTGTTGAag                                                                                                                             >  7:71162/1‑136 (MQ=255)
           cggcTACCTGGCAGGCTCTGTGGCGCCCATCGTGACCGGGTTCAGCATTCAGCAGACCGGGTCCTTCGCTTCGGCATTCCTGATCAGTGCCGCCGTGGCCGCGGGTGCTGCGATGGCTTATGTGTTGCTGTTGAag                                                                                                                             >  7:97966/1‑136 (MQ=255)
           cggcTACCTGGCAGGCTCTGTGGCGCCCATCGTGACCGGGTTCAGCATTCAGCAGACCGGCTCCTTCGCTTCGGCATTCCTGATCAGTGCCGCCGTGGCCGCGGTTGCTGCGATGGATTATGTGTTGCTGTTGAag                                                                                                                             >  2:164586/1‑136 (MQ=255)
           cggcTACCTGGCAGGCTCTGTGGCGCCCATCGTGACCGGGTTCAGCATACAGCAGACCGGGTCCTACGCTTATGAATTCCTGATCAGTGCCGCCGTGGCCGCGGGTGCTGCCATGGCTGATGTGTTGCTGTTGAag                                                                                                                             >  6:189687/1‑136 (MQ=255)
                      cAGGCTCTGTGGCGCCCATCGTGACCGGGTTCAGCATTCAGCAGACCGGGTCCTTCGCTTCGGCATTCCTGATCAGTGCCGCCGTGGCCGCGGTTGCTGCGATGGCTTATGTGTTGCTGTTGAAGCAGCCGGTTTc                                                                                                                  <  1:91139/136‑1 (MQ=255)
                              gtgGCGCCCATCGTGACCGGGTTCAGCATTCAGCAGACCGGGTCCTTCGCTTCGGCATTCCTGATCAGTGCCGCCGTGGCCGCGGTTGCTGCGATGGCTTATGTGTTGCTGTTGAAGCAGCCGGTTTCGACTGATc                                                                                                          >  4:72753/1‑136 (MQ=255)
                                         cGTGACCGGGTTCAGCATTCAGCAGACCGGGTCCTTCGCTTCGGCATTCCTGATCAGTGCCGCCGTGGCCGCGGTTGCTGCGATGGCTTATGTGTTGCTGTTGAAGCAGCCGGTTTCCACTGATCGCTGAGCATg                                                                                                <  5:65816/135‑1 (MQ=255)
                                                   ttcagcattcagcaGACCGGGTCCTTCGCTTCGGCATTCCTGATCAGTGCCGCCGTGGCCGCGGTTGCTACGATGGCTTATGTGTTGCTGTTGAAGCAGCCGGTTTCCACTGATCGCTGAGCATGCACCTGCAGGc                                                                                     <  7:45806/136‑1 (MQ=255)
                                                              gcagACCGGGTCCTTCGCTTCGGCATTCCTGATCAGTGCCGCCGTGGCCGCGGTTGCTGCGATGGCTTATGTGTTGCTGTTGAAGCAGCCGGTTTCCACTGATCGCTGAGCATGCACCTGCAGGCGGATTtcatca                                                                          <  2:54606/136‑1 (MQ=255)
                                                                agACCGGGTCCTTCGCTTCGGCATTCCTGATCAGTGCCGCCGTGGCCGCGGTTGCTGCGATGGCTTATGTGTTGCTGTTGAAGCAGCCGGTTTCCACTGATCGCTGAGCATGCACCTGCAGGCGGATTTCATCAgg                                                                        <  1:52706/136‑1 (MQ=255)
                                                                     gggTCCTTCGCTTCGGCATTCCTGATCAGTGCCGCCGTGGCCGCGGTTGCTGCGATGGCTTATGTGTTGCTGTTGAAGCAGCCGGTTTCCACTGATCGCTGAGCATGCACCTGCAGGCGGATTTCATCAGGCAGt                                                                    <  6:148121/135‑1 (MQ=255)
                                                                     gggTCCTTCGCTTCGGCATTCCTGATCAGTGCCGCCGTGGCCGCGGTTGCTGCGATGGCTTATGTGTTGCTGTTGAAGCAGCCGGTTTCCACTGATCGCTGAGCATGCACCTGCAGGCGGATTTCATCAGGCAGt                                                                    >  3:176281/1‑135 (MQ=255)
                                                                     gggTCCTTCGCTTCGGCATTCCTGATCAGTGCCGCCGTGGCCGCGGTTGCTGCGATGGCTTATGTGTTGCTGTTGAAGCAGCCGGTTTCCACTGATCGCTGAGCATGCACCTGCAGGCGGATTTCATCAGGCAGt                                                                    >  5:148121/1‑135 (MQ=255)
                                                                          cttcgcttcgGCATTCCTGATCAGTGCCGCCGTGGCCGCGGTTGCTGCGATGGCTTATGTGTTGCTGTTGAAGCAGCCGGTTTCCACTGATCGCTGAGCATGCACCTGCAGGCGGATTTCATCAGGCAGTTAAATa                                                              <  8:97966/136‑1 (MQ=255)
                                                                                  cgGCATTCCTGATCAGTGCCGCCGTGGCCGCGGTTGCTGCGATGGCTTATGTGTTGCTGTTGAAGCAGCCGGTTTCCACTGATCGCTGAGCATGCACCTGCAGGCGGATTTCATCAGGCAGTTAAATAACAAttt                                                       <  3:72753/135‑1 (MQ=255)
                                                                                  cgGCATTCCTGATCAGTGCCGCCGTGGCCGCGGTTGCTGCGATGGCTTATGTGTTGCTGTTGAAGCAGCCGGTTTCCACTGATCGCTGAGCATGCACCTGCAGGCGGATTTCATCAGGCAGTTAAATAACAAttt                                                       <  1:164586/135‑1 (MQ=255)
                                                                                  cgGCATTCCTGATCAGTGCCGCCGTGGCCGCGGTTGCTGCGATGGCTTATGTGTTGCTGTTGAAGCAGCCGGTTTCCACTGATCGCTGAGCATGCACCTGCAGGCGGATTTCATCAGGCAGTTAAATAACAAttt                                                       <  3:159485/135‑1 (MQ=255)
                                                                                       ttCCTGATCAGTGCCGCCGTGGCCGCGGTTGCTGCGATGGCTTATGTGTTGCTGTTGAAGCAGCCGGTTTCCACTGATCGCTGAGCATGCACCTGCAGGCGGATTTCATCAGGCAGTTAAATAACAATTTGtgatg                                                 <  6:20869/136‑1 (MQ=255)
                                                                                             aTCAGTGCCGCCGTGGCCGCGGTTGCTGCGATGGCTTATGTGTTGCTGTTGAAGCAGCCGGTTTCCACTGATCGCTGAGCATGCACCTGCAGGCGGATTTCATCAGGCAGTTAAATAACAATTTGTGATGATTTg                                            <  2:144507/135‑1 (MQ=255)
                                                                                              tCAGTGCCGCCGTGGCCGCGGTTGCTGCGATGGCTTATGTGTTGCTGTTGAAGCAGCCGGTTTCCACTGATCGCTGAGCATGCACCTGCAGGCGGATTTCATCAGGCAGTTAAATAACAATTTGTGATGATTTGca                                          <  1:7849/136‑1 (MQ=255)
                                                                                                aGTGCCGCCGTGGCCGCGGTTGCTGCGATGGCTTATGTGTTGCTGTTGAAGCAGCCGGTTTCCACTGATCGCTGAGCATGCACCTGCAGGCGGATTTCATCAGGCAGTTAAATAACAATTTGTGATGATTTGcatc                                        <  7:65240/136‑1 (MQ=255)
                                                                                                aGTGCCGCCGTGGCCGCGGTTGCTGCGATGGCTTATGTGTTGCTGTTGAAGCAGCCGGTTTCCACTGATCGCTGAGCATGCACCTGCAGGCGGATTTCATCAGGCAGTTAAATAACAATTTGTGATGATTTGcat                                         <  1:184463/135‑1 (MQ=255)
                                                                                                    ccgccgTGGCCGCGGTTGCTGCGATGGCTTATGTGTTGCTGTTGAAGCAGCCGGTTTCCACTGATCGCTGAGCATGCACCTGCAGGCGGATTTCATCAGGCAGTTAAATAACAATTTGTGATGATTTGCATCATGa                                    <  3:170964/136‑1 (MQ=255)
                                                                                                    ccgccgTGGCCGCGGTAGCTTCTATGGCTTATGTGTTGCTGTTGAAGCAGCCGGGTTCCACTGATCGCTGAGCATGCACCTGCAGTCGGATTTCATCAGGCAGTTAAAAAACAATTTGTGATGATTTGCATCATGa                                    <  4:115099/136‑1 (MQ=255)
                                                                                                     cgccgTGGCCGCGGTTGCTGCGATGGCTTATGTTTTGCTGTTGAAGCAGCCGGTTTCCACTGATCGCTGAGCATGCACCTGCAGGCGGATTTCATCAGGCAGTTAAATAACAATTTGTGATGATTTGCATCATGa                                    >  8:195580/1‑135 (MQ=255)
                                                                                                     cgccgTGGCCGCGGTTGCTGCGATGGCTTATGTTTTGCTGTTGAAGCAGCCGGTTTCCACTGATCGCTGAGCATGCACCTGCAGGCGGATTTCATCAGGCAGTTAAATAACAATTTGTGATGATTTGCATCATGa                                    >  8:11878/1‑135 (MQ=255)
                                                                                                     cgccgTGGCCGCGGTTGCTGCGATGGCTTATGTGTTGCTGTTGAAGCAGCCGGTTTCCACTGATCGCTGAGCATGCACCTGCAGGCGGATTTCATCAGGCAGTTAAATAACAATTTGTGATGATTTGCATCATGa                                    >  1:138583/1‑135 (MQ=255)
                                                                                                     cgccgTGGCCGCGGTTGCTGCGATGGCTTATGTGTTGCTGTTGAAGCAGCCGGTTTCCACTGATCGCTGAGCATGCACCTGCAGGCGGATTTCATCAGGCAGTTAAATAACAATTTGTGATGATTTGCATCATGa                                    >  1:150661/1‑135 (MQ=255)
                                                                                                          tGGCCGCGGTTGCTGCGATGGCTTATGTGTTGCTGGTGAAGCAGCCGTGGGCCACTGAGCGCTTAGCAGGCACCGGCACGCGGATTTCATCAGGCAGGTAAATATCAATTTGTGATTATTTGCATCATGACaaga                               >  4:56649/1‑133 (MQ=255)
                                                                                                            gCCGCGGTTGCTGCGATGGCTTATGTGTTGCTGTTGAAGCAGCCGGTTTCCACTGATCGCTGAGCATGCACCTGCAGGCGGATTTCATCAGGCAGTTAAATAACAATTTGTGATGATTTGCATCATGACAatatat                            <  2:28539/136‑1 (MQ=255)
                                                                                                              cgcgGTTGCTGCGATGGCTTATGTGTTGCTGTTGAAGCAGCCGGTTTCCACTGATCGCTGAGCATGCACCTGCAGGCGGATTTCATCAGGCAGTTAAATAACAATTTGTGATGATTTGCATCATGACAATATAttt                          <  8:183428/136‑1 (MQ=255)
                                                                                                               gcgGTTGCTGCGATGGCTTATGTGTTGCTGTTGAAGCAGCCGGTTTCCACTGATCGCTGAGCATGCACCTGCAGGCGGATTTCATCAGGCAGTTAAATAACAAttt                                                       <  3:180664/106‑1 (MQ=255)
                                                                                                               gcgGTTGCTGCGATGGCTTATGTGTTGCTGTTGAAGCAGCCGGTTTCCACTGATCGCTGAGCATGCACCTGCAGGCGGATTTCATCAGGCAGTTAAATAACAAttt                                                       >  4:180664/1‑106 (MQ=255)
                                                                                                                   ttGCTGCGATGGCTTATGTGTTGCTGTTGAAGCAGCCGGTTTCCACTGATCGCTGAGCATGCACCTGCAGGCGGATTTCATCAGGCAGTTAAATAACAATTTGTGATGATTTGCATCATGACAATATATTTATCCt                     <  5:204109/136‑1 (MQ=255)
                                                                                                                          gATGGCTTATGTGTTGCTGTTGAAGCAGCCGGTTTCCACTGATCGCTGAGCATGCACCTGCAGGCGGATTTCATCAGGCAGTTAAATAACAATTTGTGATGATTTGCATCATGACAATATATTTATCCTTATCAAt              <  4:56763/136‑1 (MQ=255)
                                                                                                                                  aTGTTTTGCTGTTGAAGCAGCCGGTTTCCACTGATCGCTGAGCATGCACCTGCAGGCGGATTTCATCAGGCAGTTAAATAACAATTTGTGATGATTTGCATCATGACAATATATTTATCCTTATCAATGTCAGGc       <  7:195580/135‑1 (MQ=255)
                                                                                                                                  aTGTTTTGCTGTTGAAGCAGCCGGTTTCCACTGATCGCTGAGCATGCACCTGCAGGCGGATTTCATCAGGCAGTTAAATAACAATTTGTGATGATTTGCATCATGACAATATATTTATCCTTATCAATGTCAGGc       <  7:11878/135‑1 (MQ=255)
                                                                                                                                      gtTGCTGTTGAAGCAGCCGGTTTCCACTGATCGCTGAGCATGCACCTGCAGGCGGATTTCATCAGGCAGTTAAATAACAATTTGTGATGATTTGCATCATGACAATATATTTATCCTTATCAATGTCAGGCCCTAg  >  6:168175/1‑136 (MQ=255)
                                                                                                                                      |                                                                                                                                       
CAGAACTTCGGCGGCTACCTGGCAGGCTCTGTGGCGCCCATCGTGACCGGGTTCAGCATTCAGCAGACCGGGTCCTTCGCTTCGGCATTCCTGATCAGTGCCGCCGTGGCCGCGGTTGCTGCGATGGCTTATGTGTTGCTGTTGAAGCAGCCGGTTTCCACTGATCGCTGAGCATGCACCTGCAGGCGGATTTCATCAGGCAGTTAAATAACAATTTGTGATGATTTGCATCATGACAATATATTTATCCTTATCAATGTCAGGCCCTAG  >  ACB122/3033146‑3033415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑