| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 96,480 | T→G | 47.3% | intergenic (+121/‑72) | cynT → / → CJ019_00104 | Carbonic anhydrase 1/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 96,480 | 0 | T | G | 47.3% | 7.9 / 3.6 | 29 | intergenic (+121/‑72) | cynT/CJ019_00104 | Carbonic anhydrase 1/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (4/10); new base G (9/5); total (14/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCCGCTACTAAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACATTACACAGTTGAAAGCGGCCCTGCCGCGTGAGTTGCTGGCGTCGGTG > ACB122/96348‑96605 | ggCCGCTACTAAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTgcg < 3:129037/136‑1 (MQ=255) ctactaAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCtt < 8:108140/136‑1 (MQ=255) ctactaAGCGACCCGGCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCtt < 2:5727/136‑1 (MQ=255) tactaAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCGTCGGCTGGCTTGAATTCCCGGACTGCCCTGCCGGCGTGCTCGCGGGCGGCCCCCGCCGgcgggcggg > 7:165878/1‑133 (MQ=255) tactaAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCGTCGGCTGCCGTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCGGCGCGCGTg > 2:70946/1‑136 (MQ=255) gTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCGGCGCGCGTGTCAGGGGCCAACg > 5:72668/1‑136 (MQ=255) ccgccgCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCGTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCGGCGCGCGGGGCAGGGGGAAACggg > 2:155870/1‑134 (MQ=255) cTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGGGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGACAGGGGCAAACGTGCCCACg < 4:77334/136‑1 (MQ=255) aGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCGTCGGCTGCCTTGAATTACCTGACTGCCCGGCCGGCGTGCTCGCTGGCGGCCCGCCCCGGCGGCCTTGTCAGGGGGAAACCTGCCCCCGGc > 8:53591/1‑136 (MQ=255) gCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGc > 4:41309/1‑136 (MQ=255) ggCGCGGCCTTCGGGTGCGTTGAATTCCCTGACTGCCCTGCGGGCGTGCTCGGTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGAAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAac < 7:14279/136‑1 (MQ=255) cgcgGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACg > 7:48752/1‑96 (MQ=255) cgcgGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCGGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGCATGGCCGTGCGACGAGCGTAAGGAGCAaccc > 8:136772/1‑134 (MQ=255) cgcgGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCGGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAacac < 2:43134/136‑1 (MQ=255) cgcgGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCCCGCCGGCGCGCTTGTCAGGGGCCAACGTGCCCACGGCCCGGGGTATGGCCGTGCGACAAGAGTAAGGAGAAacaa > 6:95575/1‑135 (MQ=255) cgcgGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCGGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACg < 8:48752/96‑1 (MQ=255) cgcgGCCTTAGGGTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCGGCGCGGTTGTGAGGGGGAAACGTGCCCACGGCCAGGGGCATGGCCGTGCGCCGAGAGAAAGGAGAAgcac > 6:190933/1‑136 (MQ=255) ggCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCGCGCTGGCGGCCCGCGCCTGCGCGGTTGTCAGGGGCAAACGTCCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGt < 1:70946/136‑1 (MQ=255) ggCTGCGTTGAATCCCCTCCGTGCCCGGCGGGGGGGCGCGCGGGCGGCCCGCGCGGGCGCGAGGGTCAGGGGCAACCGGGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACAtt < 2:202271/136‑1 (MQ=255) ggCTGCCTTTAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAACCGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACAtt < 7:83314/136‑1 (MQ=255) ggCTGCCTTGAATTCCCTGAGGGCCCTGCCGGGGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGAAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACAtt < 2:67144/136‑1 (MQ=255) ggCTGCCTTGAATGCCCTGACTGCCCTGCCGGCGTGCTCGCGGGCGGCCCGGGCCGGCGCGCTTGTCCGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACAtt < 1:109746/136‑1 (MQ=255) tGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACg > 7:137043/1‑78 (MQ=255) tGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACg < 8:137043/78‑1 (MQ=255) gACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTTCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACATTACACAGTTGAAAGCGGcc > 7:23305/1‑136 (MQ=255) gtgccGGCGTGGGCGGGGGGGGCCCGGCCCGGCGGGCTGGCCCGGCGAAACCGGCCCCACGGCCAGGGGACTGGCCGTGCGACTAGAGAAAGGAGAAACCCGGTGAACATTACACAGTTGAAAGCGGCCCTGCcgc < 6:3043/135‑1 (MQ=255) tgccGGCGTGCTCGCTGGCGGCCCGCGCCAGCGCGCTTGTCAGGGGCAACCGTGCCCACGGCCCGGGGAATGGCCGTGCGACAAGAGTAAGGAGACACACGGTGAAAATTACACAGGTGAAAGCAGCCCGGCcccg > 8:43874/1‑136 (MQ=255) ccGGCGTGCTCGCTGGCGGCCCGCGCCGGGGCGGGGGTGCGGGGCAAAACGGCCCCCGGCCCCGGGCATGGCCGGGGGGCGAGAGGAACGCGCAACACGGGGCAAATTACACAGTTGAAAGCCGGCCAGCcgcggg > 4:130185/1‑134 (MQ=255) ggcGCGCGCCGGCGGGCTTGTCAGTGGCAAAGGTGCCCACGGCCAGGGTAATGGCGGTGCGACTAGTGAAAGGAGAAACAGGGTGAACATTACACAGTTGAAAGCGGCCCTGCCGCGTGAGTTGCTGGCGTCggtg < 7:120771/136‑1 (MQ=255) | GGCCGCTACTAAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACATTACACAGTTGAAAGCGGCCCTGCCGCGTGAGTTGCTGGCGTCGGTG > ACB122/96348‑96605 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |